ALKBH8 [The Human Chemical-Genetic Interaction Map Project]

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======= ALKBH8 =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: ALKBH8
  * **<color #00a2e8>Official Name</color>**: alkB homolog 8, tRNA methyltransferase
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=91801|91801]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/Q96BT7|Q96BT7]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=ALKBH8&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20ALKBH8|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/613306|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**: N/A
  * **<color #00a2e8>UniProt Summary</color>**:  Catalyzes the methylation of 5-carboxymethyl uridine to 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in tRNA via its methyltransferase domain (PubMed:20123966, PubMed:20308323). Catalyzes the last step in the formation of 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in target tRNA (PubMed:20123966, PubMed:20308323). Has a preference for tRNA(Arg) and tRNA(Glu), and does not bind tRNA(Lys)(PubMed:20308323). Binds tRNA and catalyzes the iron and alpha-ketoglutarate dependent hydroxylation of 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in tRNA via its dioxygenase domain, giving rise to 5-(S)-methoxycarbonylhydroxymethyluridine; has a preference for tRNA(Gly) (PubMed:21285950). Required for normal survival after DNA damage (PubMed:20308323). May inhibit apoptosis and promote cell survival and angiogenesis (PubMed:19293182). {ECO:0000269|PubMed:19293182, ECO:0000269|PubMed:20123966, ECO:0000269|PubMed:20308323, ECO:0000269|PubMed:21285950}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|Methyltransf 11|
|DUF1891|
|2OG-FeII Oxy|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|tRNA (uracil) methyltransferase activity|
|tRNA wobble uridine modification|
|2-oxoglutarate-dependent dioxygenase activity|
|tRNA wobble base modification|
|tRNA methylation|
|tRNA binding|
|RNA methylation|
|tRNA modification|
|tRNA processing|
|iron ion binding|
|RNA modification|
|tRNA metabolic process|
|macromolecule methylation|
|nuclear body|
|methylation|
|ncRNA processing|
|ncRNA metabolic process|
|cellular response to DNA damage stimulus|
|zinc ion binding|
|RNA processing|
|oxidation-reduction process|
|RNA metabolic process|
|cellular response to stress|
|gene expression|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp1|5-Fluorouracil 2μM R00 exp1]]|-2.39|
|[[:results:exp453|B02 10μM R08 exp453]]|-1.96|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:c:ctu2|CTU2]]|0.495|
|[[:human genes:u:urm1|URM1]]|0.479|
|[[:human genes:c:ctu1|CTU1]]|0.467|
|[[:human genes:k:kti12|KTI12]]|0.462|
|[[:human genes:n:nr2c2ap|NR2C2AP]]|0.461|
|[[:human genes:e:elp3|ELP3]]|0.446|
|[[:human genes:m:mocs3|MOCS3]]|0.438|
|[[:human genes:e:elp6|ELP6]]|0.43|
|[[:human genes:c:c7orf26|C7orf26]]|0.41|
|[[:human genes:d:dph3|DPH3]]|0.401|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 0/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|0/28|
|blood|0/28|
|bone|0/26|
|breast|0/33|
|central nervous system|0/56|
|cervix|0/4|
|colorectal|0/17|
|esophagus|0/13|
|fibroblast|0/1|
|gastric|0/16|
|kidney|0/21|
|liver|0/20|
|lung|0/75|
|lymphocyte|0/16|
|ovary|0/26|
|pancreas|0/24|
|peripheral nervous system|0/16|
|plasma cell|0/15|
|prostate|0/1|
|skin|0/24|
|soft tissue|0/9|
|thyroid|0/2|
|upper aerodigestive|0/22|
|urinary tract|0/29|
|uterus|0/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 17650
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 4.73 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='ALKBH8 Expression in NALM6 Cells: 4.73'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>