APC [The Human Chemical-Genetic Interaction Map Project]

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======= APC =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: APC
  * **<color #00a2e8>Official Name</color>**: APC regulator of WNT signaling pathway
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=324|324]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/P25054|P25054]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=APC&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20APC|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/611731|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**:  This gene encodes a tumor suppressor protein that acts as an antagonist of the Wnt signaling pathway. It is also involved in other processes including cell migration and adhesion, transcriptional activation, and apoptosis. Defects in this gene cause familial adenomatous polyposis (FAP), an autosomal dominant pre-malignant disease that usually progresses to malignancy. Disease-associated mutations tend to be clustered in a small region designated the mutation cluster region (MCR) and result in a truncated protein product. [provided by RefSeq, Jul 2008]. 
  * **<color #00a2e8>UniProt Summary</color>**:  Tumor suppressor. Promotes rapid degradation of CTNNB1 and participates in Wnt signaling as a negative regulator. APC activity is correlated with its phosphorylation state. Activates the GEF activity of SPATA13 and ARHGEF4. Plays a role in hepatocyte growth factor (HGF)-induced cell migration. Required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells. Acts as a mediator of ERBB2-dependent stabilization of microtubules at the cell cortex. It is required for the localization of MACF1 to the cell membrane and this localization of MACF1 is critical for its function in microtubule stabilization. {ECO:0000269|PubMed:10947987, ECO:0000269|PubMed:17599059, ECO:0000269|PubMed:19151759, ECO:0000269|PubMed:19893577, ECO:0000269|PubMed:20937854}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|EB1 binding|
|APC basic|
|APC 15aa|
|APC crr|
|Suppressor APC|
|Arm|
|SAMP|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|beta-catenin destruction complex assembly|
|positive regulation of protein localization to centrosome|
|regulation of protein localization to centrosome|
|protein kinase regulator activity|
|beta-catenin destruction complex|
|Wnt signalosome|
|regulation of attachment of spindle microtubules to kinetochore|
|gamma-catenin binding|
|positive regulation of pseudopodium assembly|
|regulation of pseudopodium assembly|
|microtubule plus-end binding|
|beta-catenin destruction complex disassembly|
|dynein complex binding|
|mitotic spindle assembly checkpoint|
|spindle checkpoint|
|mitotic spindle checkpoint|
|spindle assembly checkpoint|
|negative regulation of mitotic metaphase/anaphase transition|
|negative regulation of metaphase/anaphase transition of cell cycle|
|negative regulation of microtubule depolymerization|
|negative regulation of mitotic sister chromatid separation|
|negative regulation of chromosome separation|
|negative regulation of cyclin-dependent protein serine/threonine kinase activity|
|regulation of microtubule depolymerization|
|catenin complex|
|negative regulation of cyclin-dependent protein kinase activity|
|negative regulation of mitotic sister chromatid segregation|
|negative regulation of sister chromatid segregation|
|negative regulation of chromosome segregation|
|bicellular tight junction assembly|
|tight junction assembly|
|negative regulation of microtubule polymerization or depolymerization|
|tight junction organization|
|negative regulation of mitotic nuclear division|
|apical junction assembly|
|negative regulation of nuclear division|
|regulation of mitotic metaphase/anaphase transition|
|cytoplasmic microtubule|
|regulation of metaphase/anaphase transition of cell cycle|
|lateral plasma membrane|
|regulation of mitotic sister chromatid separation|
|negative regulation of protein depolymerization|
|regulation of chromosome separation|
|mitotic cytokinesis|
|regulation of mitotic sister chromatid segregation|
|negative regulation of protein complex disassembly|
|regulation of microtubule polymerization or depolymerization|
|regulation of sister chromatid segregation|
|regulation of protein depolymerization|
|beta-catenin binding|
|kinetochore|
|cell fate specification|
|insulin receptor signaling pathway|
|canonical Wnt signaling pathway|
|ruffle membrane|
|cytoskeleton-dependent cytokinesis|
|positive regulation of cold-induced thermogenesis|
|regulation of cyclin-dependent protein serine/threonine kinase activity|
|cytokinesis|
|positive regulation of plasma membrane bounded cell projection assembly|
|regulation of chromosome segregation|
|regulation of cyclin-dependent protein kinase activity|
|cell-cell adherens junction|
|cell-cell junction assembly|
|regulation of protein complex disassembly|
|bicellular tight junction|
|negative regulation of supramolecular fiber organization|
|negative regulation of protein serine/threonine kinase activity|
|negative regulation of chromosome organization|
|cell-cell junction organization|
|negative regulation of cytoskeleton organization|
|regulation of cold-induced thermogenesis|
|cell cycle arrest|
|mitotic cell cycle checkpoint|
|cellular response to insulin stimulus|
|cell junction assembly|
|regulation of mitotic nuclear division|
|negative regulation of canonical Wnt signaling pathway|
|lamellipodium|
|regulation of microtubule cytoskeleton organization|
|regulation of nuclear division|
|cell cycle checkpoint|
|regulation of plasma membrane bounded cell projection assembly|
|regulation of cell projection assembly|
|negative regulation of Wnt signaling pathway|
|negative regulation of mitotic cell cycle phase transition|
|cell junction organization|
|positive regulation of protein catabolic process|
|regulation of microtubule-based process|
|negative regulation of protein kinase activity|
|response to insulin|
|negative regulation of cell cycle phase transition|
|microtubule binding|
|protein-containing complex disassembly|
|negative regulation of kinase activity|
|cell fate commitment|
|cellular response to peptide hormone stimulus|
|negative regulation of transferase activity|
|regulation of canonical Wnt signaling pathway|
|protein deubiquitination|
|ubiquitin protein ligase binding|
|protein modification by small protein removal|
|negative regulation of mitotic cell cycle|
|cadherin binding|
|microtubule|
|positive regulation of cellular protein localization|
|negative regulation of cell cycle process|
|cellular response to peptide|
|protein homooligomerization|
|regulation of chromosome organization|
|regulation of supramolecular fiber organization|
|cell-cell signaling by wnt|
|Wnt signaling pathway|
|regulation of Wnt signaling pathway|
|negative regulation of organelle organization|
|positive regulation of cell projection organization|
|regulation of protein catabolic process|
|response to peptide hormone|
|cellular component disassembly|
|negative regulation of protein phosphorylation|
|regulation of mitotic cell cycle phase transition|
|cell surface receptor signaling pathway involved in cell-cell signaling|
|positive regulation of catabolic process|
|pattern specification process|
|negative regulation of phosphorylation|
|regulation of cell cycle phase transition|
|protein kinase binding|
|response to peptide|
|centrosome|
|cell division|
|positive regulation of cell migration|
|transmembrane receptor protein tyrosine kinase signaling pathway|
|regulation of protein serine/threonine kinase activity|
|protein complex oligomerization|
|positive regulation of cellular component biogenesis|
|positive regulation of cell motility|
|positive regulation of cellular component movement|
|regulation of cellular protein localization|
|regulation of cytoskeleton organization|
|positive regulation of locomotion|
|negative regulation of phosphate metabolic process|
|negative regulation of phosphorus metabolic process|
|negative regulation of cell cycle|
|negative regulation of protein modification process|
|mitotic cell cycle process|
|cellular response to organonitrogen compound|
|cellular response to hormone stimulus|
|regulation of mitotic cell cycle|
|positive regulation of apoptotic process|
|positive regulation of programmed cell death|
|cellular response to nitrogen compound|
|negative regulation of cell population proliferation|
|mitotic cell cycle|
|regulation of plasma membrane bounded cell projection organization|
|positive regulation of cell death|
|perinuclear region of cytoplasm|
|negative regulation of cellular component organization|
|regulation of cell projection organization|
|enzyme linked receptor protein signaling pathway|
|regulation of cell cycle process|
|cellular response to DNA damage stimulus|
|negative regulation of catalytic activity|
|regulation of protein kinase activity|
|regulation of cell migration|
|regulation of kinase activity|
|response to hormone|
|regulation of cell motility|
|regulation of cellular localization|
|cell adhesion|
|biological adhesion|
|regulation of cellular component biogenesis|
|cell migration|
|regulation of transferase activity|
|regulation of locomotion|
|protein modification by small protein conjugation or removal|
|regulation of cellular component movement|
|regulation of catabolic process|
|cell cycle process|
|response to organonitrogen compound|
|regulation of protein localization|
|negative regulation of cellular protein metabolic process|
|cellular response to oxygen-containing compound|
|identical protein binding|
|cell motility|
|localization of cell|
|response to nitrogen compound|
|negative regulation of protein metabolic process|
|cell-cell signaling|
|negative regulation of molecular function|
|regulation of cell cycle|
|positive regulation of cellular component organization|
|cellular response to endogenous stimulus|
|negative regulation of signal transduction|
|proteolysis|
|regulation of organelle organization|
|locomotion|
|cell cycle|
|negative regulation of cell communication|
|negative regulation of signaling|
|regulation of protein phosphorylation|
|response to endogenous stimulus|
|regulation of apoptotic process|
|movement of cell or subcellular component|
|response to oxygen-containing compound|
|protein-containing complex assembly|
|regulation of programmed cell death|
|regulation of phosphorylation|
|regulation of cell population proliferation|
|negative regulation of response to stimulus|
|regulation of cell death|
|cellular response to stress|
|positive regulation of protein metabolic process|
|positive regulation of multicellular organismal process|
|regulation of phosphate metabolic process|
|regulation of phosphorus metabolic process|
|regulation of cell differentiation|
|regulation of protein modification process|
|protein-containing complex subunit organization|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='default' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp290|LLY-283 2.6μM R06 exp290]]|-2|
|[[:results:exp497|Lead acetate 2000μM R08 exp497]]|1.82|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
No correlation found to any other genes in chemogenomics.
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 19/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|0/28|
|blood|0/28|
|bone|0/26|
|breast|2/33|
|central nervous system|2/56|
|cervix|0/4|
|colorectal|2/17|
|esophagus|0/13|
|fibroblast|0/1|
|gastric|0/16|
|kidney|0/21|
|liver|1/20|
|lung|1/75|
|lymphocyte|0/16|
|ovary|2/26|
|pancreas|1/24|
|peripheral nervous system|1/16|
|plasma cell|1/15|
|prostate|1/1|
|skin|0/24|
|soft tissue|0/9|
|thyroid|0/2|
|upper aerodigestive|0/22|
|urinary tract|0/29|
|uterus|0/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 3869
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 5.74 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='APC Expression in NALM6 Cells: 5.74'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>