DLAT [The Human Chemical-Genetic Interaction Map Project]

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======= DLAT =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: DLAT
  * **<color #00a2e8>Official Name</color>**: dihydrolipoamide S-acetyltransferase
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=1737|1737]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/P10515|P10515]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=DLAT&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20DLAT|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/608770|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**:  This gene encodes component E2 of the multi-enzyme pyruvate dehydrogenase complex (PDC). PDC resides in the inner mitochondrial membrane and catalyzes the conversion of pyruvate to acetyl coenzyme A. The protein product of this gene, dihydrolipoamide acetyltransferase, accepts acetyl groups formed by the oxidative decarboxylation of pyruvate and transfers them to coenzyme A. Dihydrolipoamide acetyltransferase is the antigen for antimitochondrial antibodies. These autoantibodies are present in nearly 95% of patients with the autoimmune liver disease primary biliary cirrhosis (PBC). In PBC, activated T lymphocytes attack and destroy epithelial cells in the bile duct where this protein is abnormally distributed and overexpressed. PBC enventually leads to cirrhosis and liver failure. Mutations in this gene are also a cause of pyruvate dehydrogenase E2 deficiency which causes primary lactic acidosis in infancy and early childhood.[provided by RefSeq, Oct 2009]. 
  * **<color #00a2e8>UniProt Summary</color>**:  The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|2-oxoacid dh|
|E3 binding|
|Biotin lipoyl|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|dihydrolipoyllysine-residue acetyltransferase activity|
|mitochondrial pyruvate dehydrogenase complex|
|pyruvate dehydrogenase (NAD+) activity|
|pyruvate dehydrogenase complex|
|acetyl-CoA biosynthetic process from pyruvate|
|sleep|
|acetyl-CoA biosynthetic process|
|acetyl-CoA metabolic process|
|tricarboxylic acid cycle|
|thioester biosynthetic process|
|acyl-CoA biosynthetic process|
|ribonucleoside bisphosphate biosynthetic process|
|nucleoside bisphosphate biosynthetic process|
|purine nucleoside bisphosphate biosynthetic process|
|pyruvate metabolic process|
|aerobic respiration|
|thioester metabolic process|
|acyl-CoA metabolic process|
|glucose metabolic process|
|nucleoside bisphosphate metabolic process|
|purine nucleoside bisphosphate metabolic process|
|ribonucleoside bisphosphate metabolic process|
|coenzyme biosynthetic process|
|purine ribonucleotide biosynthetic process|
|hexose metabolic process|
|purine nucleotide biosynthetic process|
|ribonucleotide biosynthetic process|
|cellular respiration|
|ribose phosphate biosynthetic process|
|purine-containing compound biosynthetic process|
|sulfur compound biosynthetic process|
|monosaccharide metabolic process|
|cofactor biosynthetic process|
|energy derivation by oxidation of organic compounds|
|nucleotide biosynthetic process|
|nucleoside phosphate biosynthetic process|
|coenzyme metabolic process|
|purine ribonucleotide metabolic process|
|ribonucleotide metabolic process|
|purine nucleotide metabolic process|
|ribose phosphate metabolic process|
|sulfur compound metabolic process|
|purine-containing compound metabolic process|
|mitochondrial matrix|
|generation of precursor metabolites and energy|
|cofactor metabolic process|
|nucleotide metabolic process|
|nucleoside phosphate metabolic process|
|carbohydrate metabolic process|
|amide biosynthetic process|
|monocarboxylic acid metabolic process|
|organophosphate biosynthetic process|
|nucleobase-containing small molecule metabolic process|
|carbohydrate derivative biosynthetic process|
|cellular amide metabolic process|
|organophosphate metabolic process|
|carboxylic acid metabolic process|
|oxidation-reduction process|
|oxoacid metabolic process|
|organic acid metabolic process|
|carbohydrate derivative metabolic process|
|identical protein binding|
|nucleobase-containing compound biosynthetic process|
|heterocycle biosynthetic process|
|aromatic compound biosynthetic process|
|mitochondrion|
|organic cyclic compound biosynthetic process|
|organonitrogen compound biosynthetic process|
|cellular nitrogen compound biosynthetic process|
|small molecule metabolic process|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp226|Cerivastatin 0.15μM R05 exp226]]|-2.27|
|[[:results:exp358|FK-506 5μM R07 exp358]]|1.71|
|[[:results:exp183|IU1-C 25μM R04 exp183]]|1.72|
|[[:results:exp526|Sulforaphane 9μM R08 exp526 no dilution day6]]|1.73|
|[[:results:exp209|Deguelin 0.15μM R05 exp209]]|1.83|
|[[:results:exp525|Sulforaphane 9μM R08 exp525]]|1.9|
|[[:results:exp344|Chlorpromazine 10μM R07 exp344]]|1.92|
|[[:results:exp531|THZ1 0.06μM R08 exp531]]|1.92|
|[[:results:exp256|HMS-I1 10μM R06 exp256]]|1.94|
|[[:results:exp458|Bisphenol S 100μM R08 exp458]]|2.03|
|[[:results:exp275|Citral 75μM R06 exp275]]|2.08|
|[[:results:exp413|THZ531 0.11 to 0.175μM on day4 R07 exp413]]|2.19|
|[[:results:exp69|Deguelin 0.05μM R02 exp69]]|2.22|
|[[:results:exp431|Rotenone 0.07μM R08 exp431]]|2.22|
|[[:results:exp102|Nifuroxazide 5μM R03 exp102]]|5.15|
|[[:results:exp274|Citral 50μM R06 exp274]]|5.21|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:p:pdha1|PDHA1]]|0.614|
|[[:human genes:p:pdhb|PDHB]]|0.577|
|[[:human genes:m:mpc1|MPC1]]|0.513|
|[[:human genes:p:pdhx|PDHX]]|0.488|
|[[:human genes:c:cs|CS]]|0.466|
|[[:human genes:h:hgc6.3|HGC6.3]]|0.455|
|[[:human genes:g:gpt2|GPT2]]|0.4|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 0/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|0/28|
|blood|0/28|
|bone|0/26|
|breast|0/33|
|central nervous system|0/56|
|cervix|0/4|
|colorectal|0/17|
|esophagus|0/13|
|fibroblast|0/1|
|gastric|0/16|
|kidney|0/21|
|liver|0/20|
|lung|0/75|
|lymphocyte|0/16|
|ovary|0/26|
|pancreas|0/24|
|peripheral nervous system|0/16|
|plasma cell|0/15|
|prostate|0/1|
|skin|0/24|
|soft tissue|0/9|
|thyroid|0/2|
|upper aerodigestive|0/22|
|urinary tract|0/29|
|uterus|0/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 11760
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 6.62 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='DLAT Expression in NALM6 Cells: 6.62'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>