DPH1 [The Human Chemical-Genetic Interaction Map Project]

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======= DPH1 =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: DPH1
  * **<color #00a2e8>Official Name</color>**: diphthamide biosynthesis 1
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=1801|1801]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/Q9BZG8|Q9BZG8]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=DPH1&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20DPH1|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/603527|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**:  The protein encoded by this gene is an enzyme involved in the biosynthesis of diphthamide, a modified histidine found only in elongation factor-2 (EEF2). Diphthamide residues in EEF2 are targeted for ADP-ribosylation by diphtheria toxin and Pseudomonas exotoxin A. Defects in this gene have been associated with both ovarian cancer and autosomal recessive intellectual disability with short stature, craniofacial, and ectodermal anomalies. [provided by RefSeq, Oct 2016]. 
  * **<color #00a2e8>UniProt Summary</color>**:  Required for the first step in the synthesis of diphthamide, a post-translational modification of histidine which occurs in translation elongation factor 2 (EEF2). When overexpressed, suppresses colony formation ability and growth rate of ovarian cancer cells. Acts also as a tumor suppressor in lung and breast cancers (By similarity). Plays a role in embryonic growth, organogenesis and postnatal survival (By similarity). {ECO:0000250|UniProtKB:Q5NCQ5, ECO:0000269|PubMed:10519411}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|Diphthamide syn|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|peptidyl-diphthamide metabolic process|
|peptidyl-diphthamide biosynthetic process from peptidyl-histidine|
|regulation of cytoplasmic translational elongation|
|peptidyl-histidine modification|
|regulation of translational elongation|
|regulation of cytoplasmic translation|
|transferase activity|
|regulation of translation|
|regulation of cellular amide metabolic process|
|posttranscriptional regulation of gene expression|
|cell junction|
|cell population proliferation|
|peptidyl-amino acid modification|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp215|Colchicine 0.009μM R05 exp215]]|-3.82|
|[[:results:exp97|BI-6727 0.0125μM R03 exp97]]|-3.29|
|[[:results:exp228|Demecolcine 0.03μM R05 exp228]]|-3.25|
|[[:results:exp190|Vincristine 0.0005μM R04 exp190]]|-3.19|
|[[:results:exp125|GSK461364A 0.005μM R03 exp125]]|-2.92|
|[[:results:exp351|Dexamethasone 0.006μM R07 exp351]]|-2.59|
|[[:results:exp352|Dexamethasone 0.006 to 0.015μM on day4 R07 exp352]]|-2.54|
|[[:results:exp35|TRAIL 5ng/ml R00 exp35]]|-2.54|
|[[:results:exp95|BI-2536 0.0042μM R03 exp95]]|-2.49|
|[[:results:exp226|Cerivastatin 0.15μM R05 exp226]]|-2.47|
|[[:results:exp179|Combretastatin A4 0.002 to 0.003μM day4 R04 exp179]]|-2.4|
|[[:results:exp243|S-trityl-L-cysteine 0.5μM R05 exp243]]|-2.4|
|[[:results:exp60|Vinblastine 0.002μM R01 exp60]]|-2.34|
|[[:results:exp146|Quinacrine 2.5μM R03 exp146]]|-2.25|
|[[:results:exp346|CoCl2 18μM R07 exp346]]|-2.19|
|[[:results:exp460|BML-284 0.09μM R08 exp460]]|-2.16|
|[[:results:exp499|LY2090314 0.003μM R08 exp499]]|-2.16|
|[[:results:exp176|Apcin 50 to 100μM on day4 R04 exp176]]|-2.13|
|[[:results:exp38|Wortmannin 5μM R00 exp38]]|-2.1|
|[[:results:exp320|ABT-702 5μM plus CoCl2 18μM R07 exp320]]|-2.09|
|[[:results:exp514|Phorbol-12-myristate-13-acetate 0.57μM R08 exp514]]|-2.04|
|[[:results:exp28|Pimelic-diphenylamide-106 5μM R00 exp28]]|-1.95|
|[[:results:exp147|Resveratrol 16μM R03 exp147]]|-1.92|
|[[:results:exp16|DABN 2μM R00 exp16]]|-1.84|
|[[:results:exp27|Pimelic-diphenylamide-106 0.5μM R00 exp27]]|-1.82|
|[[:results:exp321|ABT-702 5μM plus Deferoxamine 11μM R07 exp321]]|-1.8|
|[[:results:exp36|TRAIL 50ng/ml R00 exp36]]|-1.8|
|[[:results:exp177|Apcin 25μM plus proTAME 2μM R04 exp177]]|-1.78|
|[[:results:exp139|Nicotinamide Riboside 100μM R03 exp139]]|-1.77|
|[[:results:exp392|PT-1 25μM R07 exp392]]|-1.76|
|[[:results:exp180|Dynasore 10μM R04 exp180]]|-1.74|
|[[:results:exp19|Etoposide 1μM R00 exp19]]|-1.73|
|[[:results:exp230|Epigallocatechin gallate 20μM R05 exp230]]|-1.72|
|[[:results:exp107|UMK57 0.6μM R03 exp107]]|-1.72|
|[[:results:exp340|BN82002 4μM R07 exp340]]|-1.71|
|[[:results:exp133|MKC9989 10μM R03 exp133]]|-1.71|
|[[:results:exp209|Deguelin 0.15μM R05 exp209]]|1.72|
|[[:results:exp25|Oligomycin-A 2μM R00 exp25]]|1.72|
|[[:results:exp502|Milciclib 2μM R08 exp502]]|1.73|
|[[:results:exp478|Doxorubicin 0.02μM R08 exp478]]|1.77|
|[[:results:exp294|Nutlin-3A 1.6μM R06 exp294]]|1.8|
|[[:results:exp358|FK-506 5μM R07 exp358]]|1.81|
|[[:results:exp365|I-BRD9 4μM R07 exp365]]|1.81|
|[[:results:exp399|Salubrinal 20μM R07 exp399]]|1.85|
|[[:results:exp448|Ammonium tetrathiomolybdate 10μM R08 exp448]]|1.88|
|[[:results:exp472|CI-1040 9.5μM R08 exp472]]|1.94|
|[[:results:exp183|IU1-C 25μM R04 exp183]]|1.96|
|[[:results:exp208|Vinblastine 0.015μM R05 exp208]]|1.96|
|[[:results:exp134|MS023 2μM R03 exp134]]|1.97|
|[[:results:exp306|Rapamycin 2μM R07 exp306]]|1.97|
|[[:results:exp488|Hippuristanol 0.12μM R08 exp488]]|2.08|
|[[:results:exp116|AICAR 240μM R03 exp116]]|2.1|
|[[:results:exp497|Lead acetate 2000μM R08 exp497]]|2.13|
|[[:results:exp468|CB-5083 0.4μM R08 exp468]]|2.15|
|[[:results:exp443|SNS-032 15μM R08 exp443]]|2.16|
|[[:results:exp440|Aphidicolin 0.4μM R08 exp440]]|2.19|
|[[:results:exp441|GSK-J4 1.5μM R08 exp441]]|2.25|
|[[:results:exp451|Atovaquone 15μM R08 exp451]]|2.26|
|[[:results:exp214|2-Deoxy-D-glucose 800μM R05 exp214]]|2.31|
|[[:results:exp519|RS-1 10μM R08 exp519]]|2.33|
|[[:results:exp342|Calcium Ionophore 0.4μM R07 exp342]]|2.38|
|[[:results:exp520|Rucaparib 6.5μM R08 exp520]]|2.43|
|[[:results:exp439|QNZ 0.01μM R08 exp439]]|2.47|
|[[:results:exp269|Bisphenol A 100μM R06 exp269]]|2.48|
|[[:results:exp359|FK-506 30μM R07 exp359]]|2.68|
|[[:results:exp18|Doxycycline 10μM R00 exp18]]|2.69|
|[[:results:exp492|iCRT14 30μM R08 exp492]]|2.84|
|[[:results:exp334|All-trans-Retinoic-Acid 40μM R07 exp334]]|2.85|
|[[:results:exp505|ML-792 0.2μM R08 exp505]]|2.92|
|[[:results:exp274|Citral 50μM R06 exp274]]|3.79|
|[[:results:exp85|UM0129480 7μM R02 exp85]]|4.13|
|[[:results:exp363|GSK-J4 1-1.25μM to day4 R07 exp363]]|4.58|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:d:dph5|DPH5]]|0.759|
|[[:human genes:d:dnajc24|DNAJC24]]|0.749|
|[[:human genes:d:dph2|DPH2]]|0.726|
|[[:human genes:d:dph3|DPH3]]|0.615|
|[[:human genes:d:dph6|DPH6]]|0.602|
|[[:human genes:u:urm1|URM1]]|0.489|
|[[:human genes:e:elp6|ELP6]]|0.487|
|[[:human genes:m:mocs3|MOCS3]]|0.483|
|[[:human genes:c:ctu1|CTU1]]|0.482|
|[[:human genes:e:elp2|ELP2]]|0.468|
|[[:human genes:c:cd2bp2|CD2BP2]]|0.452|
|[[:human genes:k:kti12|KTI12]]|0.45|
|[[:human genes:m:mapkap1|MAPKAP1]]|0.449|
|[[:human genes:e:elp3|ELP3]]|0.442|
|[[:human genes:m:mettl14|METTL14]]|0.439|
|[[:human genes:c:cxorf56|CXorf56]]|0.438|
|[[:human genes:c:ctu2|CTU2]]|0.437|
|[[:human genes:p:ppp6c|PPP6C]]|0.432|
|[[:human genes:c:cd81|CD81]]|0.424|
|[[:human genes:p:pik3cd|PIK3CD]]|0.423|
|[[:human genes:a:aar2|AAR2]]|0.42|
|[[:human genes:m:msto1|MSTO1]]|0.417|
|[[:human genes:w:wdr83|WDR83]]|0.415|
|[[:human genes:p:ptar1|PTAR1]]|0.415|
|[[:human genes:c:cbll1|CBLL1]]|0.402|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 3/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|0/28|
|blood|0/28|
|bone|0/26|
|breast|0/33|
|central nervous system|0/56|
|cervix|0/4|
|colorectal|1/17|
|esophagus|0/13|
|fibroblast|0/1|
|gastric|0/16|
|kidney|0/21|
|liver|0/20|
|lung|1/75|
|lymphocyte|0/16|
|ovary|1/26|
|pancreas|0/24|
|peripheral nervous system|0/16|
|plasma cell|0/15|
|prostate|0/1|
|skin|0/24|
|soft tissue|0/9|
|thyroid|0/2|
|upper aerodigestive|0/22|
|urinary tract|0/29|
|uterus|0/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 8245
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 5.43 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='DPH1 Expression in NALM6 Cells: 5.43'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>