EIF4A3 [The Human Chemical-Genetic Interaction Map Project]

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======= EIF4A3 =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: EIF4A3
  * **<color #00a2e8>Official Name</color>**: eukaryotic translation initiation factor 4A3
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=9775|9775]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/P38919|P38919]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=EIF4A3&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20EIF4A3|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/608546|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**:  This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene is a nuclear matrix protein. Its amino acid sequence is highly similar to the amino acid sequences of the translation initiation factors eIF4AI and eIF4AII, two other members of the DEAD box protein family. [provided by RefSeq, Jul 2008]. 
  * **<color #00a2e8>UniProt Summary</color>**:  ATP-dependent RNA helicase. Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). Its RNA-dependent ATPase and RNA-helicase activities are induced by CASC3, but abolished in presence of the MAGOH-RBM8A heterodimer, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. The inhibition of ATPase activity by the MAGOH-RBM8A heterodimer increases the RNA-binding affinity of the EJC. Involved in translational enhancement of spliced mRNAs after formation of the 80S ribosome complex. Binds spliced mRNA in sequence-independent manner, 20-24 nucleotides upstream of mRNA exon-exon junctions. Shows higher affinity for single-stranded RNA in an ATP-bound core EJC complex than after the ATP is hydrolyzed. Involved in the splicing modulation of BCL2L1/Bcl-X (and probably other apoptotic genes); specifically inhibits formation of proapoptotic isoforms such as Bcl-X(S); the function is different from the established EJC assembly. Involved in craniofacial development. {ECO:0000269|PubMed:15034551, ECO:0000269|PubMed:16170325, ECO:0000269|PubMed:16209946, ECO:0000269|PubMed:17375189, ECO:0000269|PubMed:19409878, ECO:0000269|PubMed:22203037, ECO:0000269|PubMed:24360810}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|DEAD|
|Helicase C|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|negative regulation of RNA binding|
|negative regulation of mRNA binding|
|negative regulation of selenocysteine incorporation|
|response to selenite ion|
|cellular response to selenite ion|
|regulation of selenocysteine insertion sequence binding|
|regulation of selenocysteine incorporation|
|negative regulation of selenocysteine insertion sequence binding|
|selenocysteine insertion sequence binding|
|regulation of translation at postsynapse|
|regulation of translation at postsynapse, modulating synaptic transmission|
|negative regulation of translational elongation|
|regulation of translation at synapse, modulating synaptic transmission|
|regulation of translation at synapse|
|regulation of mRNA binding|
|negative regulation of excitatory postsynaptic potential|
|cellular response to brain-derived neurotrophic factor stimulus|
|regulation of RNA binding|
|U2-type catalytic step 1 spliceosome|
|postsynaptic modulation of chemical synaptic transmission|
|postsynaptic cytosol|
|translation regulator activity|
|regulation of translational elongation|
|exon-exon junction complex|
|RNA stem-loop binding|
|poly(A) binding|
|negative regulation of nervous system process|
|exploration behavior|
|ribonucleoprotein complex binding|
|modulation of excitatory postsynaptic potential|
|embryonic cranial skeleton morphogenesis|
|cellular response to nerve growth factor stimulus|
|response to nerve growth factor|
|negative regulation of synaptic transmission|
|RNA helicase activity|
|cranial skeletal system development|
|associative learning|
|mRNA 3-end processing|
|catalytic step 2 spliceosome|
|embryonic skeletal system morphogenesis|
|mRNA-containing ribonucleoprotein complex export from nucleus|
|mRNA export from nucleus|
|nuclear-transcribed mRNA catabolic process, nonsense-mediated decay|
|ribonucleoprotein complex export from nucleus|
|ribonucleoprotein complex localization|
|positive regulation of translation|
|embryonic skeletal system development|
|RNA export from nucleus|
|negative regulation of translation|
|RNA 3-end processing|
|regulation of nervous system process|
|learning|
|negative regulation of cellular amide metabolic process|
|protein export from nucleus|
|positive regulation of cellular amide metabolic process|
|mRNA transport|
|nuclear export|
|mRNA binding|
|negative regulation of binding|
|nucleic acid transport|
|RNA transport|
|establishment of RNA localization|
|nuclear-transcribed mRNA catabolic process|
|rRNA processing|
|mRNA catabolic process|
|RNA localization|
|cellular response to inorganic substance|
|rRNA metabolic process|
|skeletal system morphogenesis|
|nucleobase-containing compound transport|
|RNA catabolic process|
|protein-containing complex localization|
|learning or memory|
|nucleocytoplasmic transport|
|nuclear transport|
|embryonic organ morphogenesis|
|mRNA splicing, via spliceosome|
|RNA splicing, via transesterification reactions with bulged adenosine as nucleophile|
|cognition|
|ribosome biogenesis|
|RNA splicing, via transesterification reactions|
|glutamatergic synapse|
|regulation of translation|
|neuronal cell body|
|nucleobase-containing compound catabolic process|
|regulation of binding|
|ncRNA processing|
|RNA splicing|
|nuclear speck|
|regulation of cellular amide metabolic process|
|dendrite|
|regulation of membrane potential|
|embryonic organ development|
|heterocycle catabolic process|
|cellular nitrogen compound catabolic process|
|modulation of chemical synaptic transmission|
|aromatic compound catabolic process|
|regulation of trans-synaptic signaling|
|ribonucleoprotein complex biogenesis|
|ncRNA metabolic process|
|mRNA processing|
|organic cyclic compound catabolic process|
|skeletal system development|
|cellular response to growth factor stimulus|
|posttranscriptional regulation of gene expression|
|response to growth factor|
|response to inorganic substance|
|embryonic morphogenesis|
|behavior|
|regulation of system process|
|chordate embryonic development|
|embryo development ending in birth or egg hatching|
|mRNA metabolic process|
|nucleolus|
|RNA processing|
|cellular macromolecule catabolic process|
|response to organic cyclic compound|
|animal organ morphogenesis|
|embryo development|
|intracellular protein transport|
|negative regulation of cellular protein metabolic process|
|macromolecule catabolic process|
|negative regulation of protein metabolic process|
|negative regulation of molecular function|
|negative regulation of multicellular organismal process|
|cellular response to endogenous stimulus|
|negative regulation of signal transduction|
|negative regulation of cell communication|
|negative regulation of signaling|
|nervous system process|
|negative regulation of cellular macromolecule biosynthetic process|
|RNA binding|
|negative regulation of macromolecule biosynthetic process|
|response to endogenous stimulus|
|ATP binding|
|protein transport|
|negative regulation of cellular biosynthetic process|
|intracellular transport|
|peptide transport|
|negative regulation of biosynthetic process|
|amide transport|
|cellular protein localization|
|cellular macromolecule localization|
|positive regulation of cellular protein metabolic process|
|establishment of protein localization|
|negative regulation of response to stimulus|
|RNA metabolic process|
|positive regulation of protein metabolic process|
|negative regulation of gene expression|
|organic substance catabolic process|
|cellular catabolic process|
|establishment of localization in cell|
|nitrogen compound transport|
|positive regulation of macromolecule biosynthetic process|
|system process|
|positive regulation of cellular biosynthetic process|
|positive regulation of gene expression|
|membrane|
|gene expression|
|positive regulation of biosynthetic process|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp14|Cycloheximide 0.02μM R00 exp14]]|1.72|
|[[:results:exp47|Lapatinib 5μM R01 exp47]]|1.73|
|[[:results:exp218|A-395 10μM R05 exp218]]|1.74|
|[[:results:exp400|Senexin-A 25μM R07 exp400]]|1.76|
|[[:results:exp426|FBS-Wisent 0.1 R07 exp426]]|1.82|
|[[:results:exp226|Cerivastatin 0.15μM R05 exp226]]|1.84|
|[[:results:exp420|Tunicamycin 0.04 to 0.125μM  on day4 R07 exp420]]|1.92|
|[[:results:exp270|Campthothecin 0.001μM R06 exp270]]|2.15|
|[[:results:exp392|PT-1 25μM R07 exp392]]|2.19|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:s:supt5h|SUPT5H]]|0.533|
|[[:human genes:p:psmb4|PSMB4]]|0.524|
|[[:human genes:p:prim1|PRIM1]]|0.514|
|[[:human genes:i:iscu|ISCU]]|0.512|
|[[:human genes:r:rrp7a|RRP7A]]|0.506|
|[[:human genes:v:vasn|VASN]]|0.497|
|[[:human genes:c:ccna2|CCNA2]]|0.496|
|[[:human genes:s:snrpa1|SNRPA1]]|0.493|
|[[:human genes:c:c14orf93|C14orf93]]|0.492|
|[[:human genes:t:tceb3c|TCEB3C]]|0.492|
|[[:human genes:a:aamp|AAMP]]|0.486|
|[[:human genes:n:npipb5|NPIPB5]]|0.483|
|[[:human genes:c:ckap5|CKAP5]]|0.482|
|[[:human genes:t:tceb3cl2|TCEB3CL2]]|0.478|
|[[:human genes:l:lrr1|LRR1]]|0.476|
|[[:human genes:o:orc1|ORC1]]|0.473|
|[[:human genes:f:fam231c|FAM231C]]|0.467|
|[[:human genes:f:fam231a|FAM231A]]|0.467|
|[[:human genes:n:nup160|NUP160]]|0.467|
|[[:human genes:c:cdca3|CDCA3]]|0.467|
|[[:human genes:c:c1qtnf9b|C1QTNF9B]]|0.466|
|[[:human genes:b:brd2|BRD2]]|0.465|
|[[:human genes:c:cep192|CEP192]]|0.464|
|[[:human genes:c:cdc6|CDC6]]|0.463|
|[[:human genes:e:egr2|EGR2]]|0.458|
|[[:human genes:t:tceb3cl|TCEB3CL]]|0.458|
|[[:human genes:p:psma6|PSMA6]]|0.455|
|[[:human genes:p:psmb2|PSMB2]]|0.451|
|[[:human genes:p:paf1|PAF1]]|0.448|
|[[:human genes:d:dtl|DTL]]|0.447|
|[[:human genes:c:chaf1a|CHAF1A]]|0.446|
|[[:human genes:a:atp6v1c1|ATP6V1C1]]|0.446|
|[[:human genes:m:mt1e|MT1E]]|0.446|
|[[:human genes:t:tfip11|TFIP11]]|0.445|
|[[:human genes:s:sf3a3|SF3A3]]|0.443|
|[[:human genes:e:exosc6|EXOSC6]]|0.441|
|[[:human genes:c:copg1|COPG1]]|0.44|
|[[:human genes:s:slc6a17|SLC6A17]]|0.439|
|[[:human genes:n:nupl1|NUPL1]]|0.437|
|[[:human genes:g:golga8o|GOLGA8O]]|0.436|
|[[:human genes:p:pan2|PAN2]]|0.436|
|[[:human genes:t:tbc1d3h|TBC1D3H]]|0.436|
|[[:human genes:p:psmd3|PSMD3]]|0.435|
|[[:human genes:c:cbwd3|CBWD3]]|0.435|
|[[:human genes:p:plk4|PLK4]]|0.433|
|[[:human genes:c:cwc22|CWC22]]|0.432|
|[[:human genes:a:arcn1|ARCN1]]|0.432|
|[[:human genes:p:pds5b|PDS5B]]|0.432|
|[[:human genes:n:ndc1|NDC1]]|0.431|
|[[:human genes:p:psma7|PSMA7]]|0.43|
|[[:human genes:n:npipb15|NPIPB15]]|0.43|
|[[:human genes:s:sec13|SEC13]]|0.427|
|[[:human genes:n:nup54|NUP54]]|0.426|
|[[:human genes:p:pola1|POLA1]]|0.425|
|[[:human genes:t:tp53tg3d|TP53TG3D]]|0.424|
|[[:human genes:p:psmb3|PSMB3]]|0.423|
|[[:human genes:g:golga6l1|GOLGA6L1]]|0.422|
|[[:human genes:c:cdc45|CDC45]]|0.422|
|[[:human genes:d:dido1|DIDO1]]|0.422|
|[[:human genes:t:tbc1d3g|TBC1D3G]]|0.421|
|[[:human genes:p:polr2j3|POLR2J3]]|0.421|
|[[:human genes:o:orc6|ORC6]]|0.421|
|[[:human genes:s:snrnp70|SNRNP70]]|0.42|
|[[:human genes:a:adra1d|ADRA1D]]|0.42|
|[[:human genes:c:cgb2|CGB2]]|0.42|
|[[:human genes:c:cln6|CLN6]]|0.42|
|[[:human genes:i:ints5|INTS5]]|0.419|
|[[:human genes:i:ik|IK]]|0.419|
|[[:human genes:v:vps28|VPS28]]|0.419|
|[[:human genes:p:psmd6|PSMD6]]|0.418|
|[[:human genes:d:dock6|DOCK6]]|0.416|
|[[:human genes:t:tbc1d3c|TBC1D3C]]|0.415|
|[[:human genes:t:tinf2|TINF2]]|0.415|
|[[:human genes:t:tsg101|TSG101]]|0.415|
|[[:human genes:t:tp53tg3b|TP53TG3B]]|0.413|
|[[:human genes:r:rpl35|RPL35]]|0.413|
|[[:human genes:t:tp53tg3|TP53TG3]]|0.412|
|[[:human genes:r:rps9|RPS9]]|0.412|
|[[:human genes:t:tp53tg3c|TP53TG3C]]|0.412|
|[[:human genes:d:ddtl|DDTL]]|0.411|
|[[:human genes:p:psmd2|PSMD2]]|0.411|
|[[:human genes:p:psmc2|PSMC2]]|0.411|
|[[:human genes:r:rps19|RPS19]]|0.409|
|[[:human genes:r:rps3a|RPS3A]]|0.409|
|[[:human genes:p:psmd14|PSMD14]]|0.408|
|[[:human genes:c:cdc73|CDC73]]|0.408|
|[[:human genes:r:rps2|RPS2]]|0.407|
|[[:human genes:s:supt6h|SUPT6H]]|0.406|
|[[:human genes:p:pan3|PAN3]]|0.406|
|[[:human genes:h:hist2h2bf|HIST2H2BF]]|0.405|
|[[:human genes:r:rps5|RPS5]]|0.405|
|[[:human genes:r:rbm39|RBM39]]|0.405|
|[[:human genes:s:sart1|SART1]]|0.405|
|[[:human genes:g:golga6l3|GOLGA6L3]]|0.405|
|[[:human genes:r:rps19bp1|RPS19BP1]]|0.404|
|[[:human genes:t:ticrr|TICRR]]|0.404|
|[[:human genes:r:rsl1d1|RSL1D1]]|0.402|
|[[:human genes:r:rffl|RFFL]]|0.402|
|[[:human genes:w:wdr87|WDR87]]|0.402|
|[[:human genes:s:smg7|SMG7]]|0.402|
|[[:human genes:c:cdh24|CDH24]]|0.402|
|[[:human genes:r:rnf113a|RNF113A]]|0.402|
|[[:human genes:i:isy1|ISY1]]|0.401|
|[[:human genes:a:atp6ap1|ATP6AP1]]|0.401|
|[[:human genes:t:tbc1d3f|TBC1D3F]]|0.4|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 682/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|1/1|
|909776.0|1/1|
|bile duct|28/28|
|blood|28/28|
|bone|25/26|
|breast|31/33|
|central nervous system|51/56|
|cervix|4/4|
|colorectal|14/17|
|esophagus|12/13|
|fibroblast|1/1|
|gastric|16/16|
|kidney|18/21|
|liver|18/20|
|lung|66/75|
|lymphocyte|15/16|
|ovary|25/26|
|pancreas|24/24|
|peripheral nervous system|14/16|
|plasma cell|15/15|
|prostate|1/1|
|skin|22/24|
|soft tissue|9/9|
|thyroid|2/2|
|upper aerodigestive|16/22|
|urinary tract|24/29|
|uterus|4/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 120
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 8.07 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='EIF4A3 Expression in NALM6 Cells: 8.07'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>