EPO [The Human Chemical-Genetic Interaction Map Project]

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======= EPO =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: EPO
  * **<color #00a2e8>Official Name</color>**: erythropoietin
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=2056|2056]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/P01588|P01588]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=EPO&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20EPO|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/133170|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**:  This gene encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The encoded protein is mainly synthesized in the kidney, secreted into the blood plasma, and binds to the erythropoietin receptor to promote red blood cell production, or erythropoiesis, in the bone marrow. Expression of this gene is upregulated under hypoxic conditions, in turn leading to increased erythropoiesis and enhanced oxygen-carrying capacity of the blood. Expression of this gene has also been observed in brain and in the eye, and elevated expression levels have been observed in diabetic retinopathy and ocular hypertension. Recombinant forms of the encoded protein exhibit neuroprotective activity against a variety of potential brain injuries, as well as antiapoptotic functions in several tissue types, and have been used in the treatment of anemia and to enhance the efficacy of cancer therapies. [provided by RefSeq, Aug 2017]. 
  * **<color #00a2e8>UniProt Summary</color>**:  Hormone involved in the regulation of erythrocyte proliferation and differentiation and the maintenance of a physiological level of circulating erythrocyte mass. Binds to EPOR leading to EPOR dimerization and JAK2 activation thereby activating specific downstream effectors, including STAT1 and STAT3. {ECO:0000269|PubMed:28283061}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|EPO TPO|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|negative regulation of erythrocyte apoptotic process|
|regulation of erythrocyte apoptotic process|
|erythropoietin receptor binding|
|erythropoietin-mediated signaling pathway|
|hemoglobin biosynthetic process|
|negative regulation of intrinsic apoptotic signaling pathway in response to osmotic stress|
|cellular response to erythropoietin|
|regulation of intrinsic apoptotic signaling pathway in response to osmotic stress|
|response to erythropoietin|
|hemoglobin metabolic process|
|regulation of cellular response to osmotic stress|
|cellular hyperosmotic response|
|regulation of response to osmotic stress|
|erythrocyte maturation|
|negative regulation of myeloid cell apoptotic process|
|negative regulation of calcium ion transport into cytosol|
|response to vitamin A|
|response to hyperoxia|
|positive regulation of activated T cell proliferation|
|response to salt stress|
|hyperosmotic response|
|protein kinase activator activity|
|response to increased oxygen levels|
|regulation of myeloid cell apoptotic process|
|erythrocyte development|
|cellular response to osmotic stress|
|regulation of activated T cell proliferation|
|embryo implantation|
|response to testosterone|
|response to dexamethasone|
|acute-phase response|
|response to electrical stimulus|
|negative regulation of cation channel activity|
|response to axon injury|
|myeloid cell development|
|negative regulation of calcium ion transport|
|positive regulation of Ras protein signal transduction|
|positive regulation of tyrosine phosphorylation of STAT protein|
|acute inflammatory response|
|negative regulation of ion transmembrane transporter activity|
|positive regulation of small GTPase mediated signal transduction|
|cell body|
|response to estrogen|
|response to osmotic stress|
|regulation of transcription from RNA polymerase II promoter in response to hypoxia|
|negative regulation of transporter activity|
|regulation of tyrosine phosphorylation of STAT protein|
|erythrocyte differentiation|
|negative regulation of cation transmembrane transport|
|positive regulation of receptor signaling pathway via JAK-STAT|
|positive regulation of phosphatidylinositol 3-kinase signaling|
|response to vitamin|
|positive regulation of receptor signaling pathway via STAT|
|erythrocyte homeostasis|
|response to antineoplastic agent|
|positive regulation of T cell proliferation|
|negative regulation of intrinsic apoptotic signaling pathway|
|negative regulation of ion transmembrane transport|
|hormone activity|
|regulation of calcium ion transport into cytosol|
|myeloid cell homeostasis|
|negative regulation of transmembrane transport|
|regulation of phosphatidylinositol 3-kinase signaling|
|regulation of transcription from RNA polymerase II promoter in response to stress|
|regulation of DNA-templated transcription in response to stress|
|positive regulation of lymphocyte proliferation|
|regulation of receptor signaling pathway via JAK-STAT|
|positive regulation of mononuclear cell proliferation|
|regulation of receptor signaling pathway via STAT|
|anatomical structure maturation|
|positive regulation of leukocyte proliferation|
|response to glucocorticoid|
|negative regulation of ion transport|
|regulation of T cell proliferation|
|cell maturation|
|regulation of intrinsic apoptotic signaling pathway|
|response to corticosteroid|
|regulation of cation channel activity|
|cytokine activity|
|peptidyl-serine phosphorylation|
|female pregnancy|
|cellular response to hypoxia|
|positive regulation of peptidyl-tyrosine phosphorylation|
|response to ketone|
|cellular response to decreased oxygen levels|
|peptidyl-serine modification|
|response to interleukin-1|
|homeostasis of number of cells|
|positive regulation of T cell activation|
|negative regulation of neuron death|
|regulation of lymphocyte proliferation|
|regulation of mononuclear cell proliferation|
|multi-multicellular organism process|
|positive regulation of ERK1 and ERK2 cascade|
|cellular response to oxygen levels|
|response to nutrient|
|positive regulation of leukocyte cell-cell adhesion|
|myeloid cell differentiation|
|regulation of leukocyte proliferation|
|negative regulation of apoptotic signaling pathway|
|developmental maturation|
|regulation of Ras protein signal transduction|
|regulation of calcium ion transport|
|regulation of peptidyl-tyrosine phosphorylation|
|positive regulation of cell-cell adhesion|
|regulation of ion transmembrane transporter activity|
|regulation of transmembrane transporter activity|
|regulation of transporter activity|
|aging|
|positive regulation of neuron projection development|
|regulation of ERK1 and ERK2 cascade|
|regulation of leukocyte cell-cell adhesion|
|response to xenobiotic stimulus|
|regulation of neuron death|
|cellular response to environmental stimulus|
|cellular response to abiotic stimulus|
|response to lipopolysaccharide|
|regulation of T cell activation|
|response to molecule of bacterial origin|
|response to steroid hormone|
|activation of protein kinase activity|
|regulation of cation transmembrane transport|
|response to hypoxia|
|regulation of small GTPase mediated signal transduction|
|response to decreased oxygen levels|
|positive regulation of lymphocyte activation|
|positive regulation of neuron differentiation|
|response to oxygen levels|
|positive regulation of cell projection organization|
|regulation of metal ion transport|
|blood circulation|
|circulatory system process|
|regulation of apoptotic signaling pathway|
|regulation of cell-cell adhesion|
|positive regulation of cell adhesion|
|positive regulation of leukocyte activation|
|positive regulation of cell activation|
|positive regulation of neurogenesis|
|regulation of ion transmembrane transport|
|negative regulation of transport|
|regulation of neuron projection development|
|response to nutrient levels|
|inflammatory response|
|negative regulation of intracellular signal transduction|
|regulation of lymphocyte activation|
|response to extracellular stimulus|
|positive regulation of protein kinase activity|
|positive regulation of nervous system development|
|positive regulation of cell development|
|positive regulation of MAPK cascade|
|cell population proliferation|
|hemopoiesis|
|regulation of transmembrane transport|
|response to wounding|
|positive regulation of kinase activity|
|regulation of leukocyte activation|
|hematopoietic or lymphoid organ development|
|cell surface|
|regulation of cell activation|
|immune system development|
|positive regulation of transferase activity|
|regulation of neuron differentiation|
|cytokine-mediated signaling pathway|
|regulation of cell adhesion|
|response to bacterium|
|regulation of plasma membrane bounded cell projection organization|
|regulation of ion transport|
|regulation of cell projection organization|
|regulation of cellular response to stress|
|regulation of MAPK cascade|
|regulation of protein kinase activity|
|regulation of neurogenesis|
|response to lipid|
|negative regulation of transcription by RNA polymerase II|
|regulation of kinase activity|
|peptidyl-amino acid modification|
|negative regulation of apoptotic process|
|negative regulation of programmed cell death|
|response to hormone|
|positive regulation of cell population proliferation|
|regulation of cellular localization|
|response to organic cyclic compound|
|apoptotic process|
|regulation of nervous system development|
|regulation of cell development|
|positive regulation of cell differentiation|
|protein phosphorylation|
|regulation of transferase activity|
|multi-organism reproductive process|
|negative regulation of cell death|
|cellular response to cytokine stimulus|
|positive regulation of protein phosphorylation|
|positive regulation of intracellular signal transduction|
|response to drug|
|programmed cell death|
|positive regulation of phosphorylation|
|cell death|
|response to cytokine|
|positive regulation of phosphorus metabolic process|
|positive regulation of phosphate metabolic process|
|negative regulation of molecular function|
|positive regulation of immune system process|
|response to abiotic stimulus|
|negative regulation of transcription, DNA-templated|
|positive regulation of cellular component organization|
|positive regulation of protein modification process|
|negative regulation of nucleic acid-templated transcription|
|negative regulation of RNA biosynthetic process|
|negative regulation of signal transduction|
|phosphorylation|
|response to other organism|
|response to external biotic stimulus|
|response to biotic stimulus|
|negative regulation of RNA metabolic process|
|defense response|
|negative regulation of cell communication|
|negative regulation of signaling|
|positive regulation of developmental process|
|negative regulation of cellular macromolecule biosynthetic process|
|organonitrogen compound biosynthetic process|
|reproductive process|
|reproduction|
|positive regulation of catalytic activity|
|negative regulation of nucleobase-containing compound metabolic process|
|regulation of protein phosphorylation|
|negative regulation of macromolecule biosynthetic process|
|response to endogenous stimulus|
|regulation of response to stress|
|negative regulation of cellular biosynthetic process|
|generation of neurons|
|positive regulation of transcription, DNA-templated|
|regulation of apoptotic process|
|negative regulation of biosynthetic process|
|response to oxygen-containing compound|
|regulation of programmed cell death|
|regulation of phosphorylation|
|extracellular space|
|positive regulation of cellular protein metabolic process|
|regulation of cell population proliferation|
|negative regulation of response to stimulus|
|neurogenesis|
|positive regulation of nucleic acid-templated transcription|
|positive regulation of RNA biosynthetic process|
|homeostatic process|
|cell development|
|regulation of immune system process|
|positive regulation of signal transduction|
|regulation of cell death|
|cellular response to stress|
|positive regulation of protein metabolic process|
|cellular macromolecule biosynthetic process|
|negative regulation of gene expression|
|positive regulation of RNA metabolic process|
|positive regulation of multicellular organismal process|
|macromolecule biosynthetic process|
|positive regulation of molecular function|
|regulation of phosphate metabolic process|
|regulation of phosphorus metabolic process|
|regulation of cell differentiation|
|positive regulation of cell communication|
|positive regulation of signaling|
|regulation of intracellular signal transduction|
|regulation of protein modification process|
|regulation of transport|
|positive regulation of nucleobase-containing compound metabolic process|
|positive regulation of macromolecule biosynthetic process|
|extracellular region|
|system process|
|positive regulation of cellular biosynthetic process|
|positive regulation of gene expression|
|positive regulation of biosynthetic process|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='default' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp82|Torin1 0.08μM R02 exp82]]|-1.75|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
No correlation found to any other genes in chemogenomics.
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 0/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|0/28|
|blood|0/28|
|bone|0/26|
|breast|0/33|
|central nervous system|0/56|
|cervix|0/4|
|colorectal|0/17|
|esophagus|0/13|
|fibroblast|0/1|
|gastric|0/16|
|kidney|0/21|
|liver|0/20|
|lung|0/75|
|lymphocyte|0/16|
|ovary|0/26|
|pancreas|0/24|
|peripheral nervous system|0/16|
|plasma cell|0/15|
|prostate|0/1|
|skin|0/24|
|soft tissue|0/9|
|thyroid|0/2|
|upper aerodigestive|0/22|
|urinary tract|0/29|
|uterus|0/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 8997
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: -3.02 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='EPO Expression in NALM6 Cells: -3.02'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>