ERCC3 [The Human Chemical-Genetic Interaction Map Project]

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======= ERCC3 =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: ERCC3
  * **<color #00a2e8>Official Name</color>**: ERCC excision repair 3, TFIIH core complex helicase subunit
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=2071|2071]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/P19447|P19447]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=ERCC3&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20ERCC3|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/133510|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**:  This gene encodes an ATP-dependent DNA helicase that functions in nucleotide excision repair. The encoded protein is a subunit of basal transcription factor 2 (TFIIH) and, therefore, also functions in class II transcription. Mutations in this gene are associated with Xeroderma pigmentosum B, Cockayne's syndrome, and trichothiodystrophy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]. COMPLETENESS: complete on the 3' end. 
  * **<color #00a2e8>UniProt Summary</color>**:  ATP-dependent 3'-5' DNA helicase, component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. The ATPase activity of XPB/ERCC3, but not its helicase activity, is required for DNA opening. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. The ATP- dependent helicase activity of XPB/ERCC3 is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription. {ECO:0000269|PubMed:10024882, ECO:0000269|PubMed:8157004}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|Helicase C|
|ResIII|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|nucleotide-excision repair factor 3 complex|
|transcriptional preinitiation complex|
|transcription factor TFIIH core complex|
|DNA topological change|
|transcription factor TFIIH holo complex|
|UV protection|
|RNA polymerase II CTD heptapeptide repeat kinase activity|
|3-5 DNA helicase activity|
|nucleotide-excision repair, preincision complex stabilization|
|nucleotide-excision repair, DNA incision, 3-to lesion|
|nucleotide-excision repair, DNA duplex unwinding|
|global genome nucleotide-excision repair|
|nucleotide-excision repair, preincision complex assembly|
|transcription elongation from RNA polymerase I promoter|
|termination of RNA polymerase I transcription|
|7-methylguanosine mRNA capping|
|hair cell differentiation|
|helicase activity|
|7-methylguanosine RNA capping|
|RNA capping|
|transcription initiation from RNA polymerase I promoter|
|nucleotide-excision repair, DNA incision, 5-to lesion|
|transcription factor TFIID complex|
|transcription by RNA polymerase I|
|nucleotide-excision repair, DNA incision|
|DNA-dependent ATPase activity|
|damaged DNA binding|
|DNA helicase activity|
|DNA-templated transcription, termination|
|transcription-coupled nucleotide-excision repair|
|transcription elongation from RNA polymerase II promoter|
|DNA-templated transcription, elongation|
|protein N-terminus binding|
|nucleotide-excision repair|
|DNA duplex unwinding|
|DNA geometric change|
|response to UV|
|transcription initiation from RNA polymerase II promoter|
|protein C-terminus binding|
|protein-DNA complex assembly|
|DNA-templated transcription, initiation|
|ATPase activity|
|protein kinase activity|
|protein-DNA complex subunit organization|
|positive regulation of protein complex assembly|
|DNA conformation change|
|nucleic acid phosphodiester bond hydrolysis|
|response to light stimulus|
|epidermal cell differentiation|
|transcription factor binding|
|response to hypoxia|
|response to decreased oxygen levels|
|response to oxygen levels|
|response to oxidative stress|
|regulation of mitotic cell cycle phase transition|
|epidermis development|
|embryonic organ development|
|response to radiation|
|regulation of cell cycle phase transition|
|regulation of protein complex assembly|
|mRNA processing|
|transcription by RNA polymerase II|
|DNA repair|
|positive regulation of cellular component biogenesis|
|regulation of mitotic cell cycle|
|transcription, DNA-templated|
|nucleic acid-templated transcription|
|positive regulation of apoptotic process|
|positive regulation of programmed cell death|
|RNA biosynthetic process|
|epithelial cell differentiation|
|mRNA metabolic process|
|positive regulation of cell death|
|viral process|
|DNA metabolic process|
|regulation of cell cycle process|
|cellular response to DNA damage stimulus|
|symbiotic process|
|interspecies interaction between organisms|
|cellular protein-containing complex assembly|
|RNA processing|
|apoptotic process|
|regulation of cellular component biogenesis|
|protein phosphorylation|
|embryo development|
|neuron differentiation|
|programmed cell death|
|chromosome organization|
|cell death|
|nucleobase-containing compound biosynthetic process|
|epithelium development|
|response to abiotic stimulus|
|heterocycle biosynthetic process|
|aromatic compound biosynthetic process|
|regulation of cell cycle|
|positive regulation of cellular component organization|
|positive regulation of transcription by RNA polymerase II|
|phosphorylation|
|organic cyclic compound biosynthetic process|
|DNA binding|
|ATP binding|
|generation of neurons|
|regulation of apoptotic process|
|positive regulation of transcription, DNA-templated|
|protein-containing complex assembly|
|regulation of programmed cell death|
|neurogenesis|
|cellular nitrogen compound biosynthetic process|
|positive regulation of nucleic acid-templated transcription|
|positive regulation of RNA biosynthetic process|
|RNA metabolic process|
|regulation of cell death|
|cellular response to stress|
|cellular macromolecule biosynthetic process|
|positive regulation of RNA metabolic process|
|tissue development|
|macromolecule biosynthetic process|
|protein-containing complex subunit organization|
|positive regulation of nucleobase-containing compound metabolic process|
|positive regulation of macromolecule biosynthetic process|
|positive regulation of cellular biosynthetic process|
|positive regulation of gene expression|
|gene expression|
|positive regulation of biosynthetic process|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='default' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp3|Actinomycin-D 0.001μM R00 exp3]]|-2.29|
|[[:results:exp508|NN-Dimethylsphingosine 2.5μM R08 exp508]]|-1.98|
|[[:results:exp299|Talazoparib 0.006μM R06 exp299]]|-1.97|
|[[:results:exp270|Campthothecin 0.001μM R06 exp270]]|-1.93|
|[[:results:exp38|Wortmannin 5μM R00 exp38]]|-1.86|
|[[:results:exp274|Citral 50μM R06 exp274]]|-1.78|
|[[:results:exp459|Bleomycin 5μM R08 exp459]]|-1.76|
|[[:results:exp28|Pimelic-diphenylamide-106 5μM R00 exp28]]|-1.74|
|[[:results:exp77|Prochlorperazine 5.2μM R02 exp77]]|-1.73|
|[[:results:exp533|TNF-alpha 44ng/ml R08 exp533]]|1.9|
|[[:results:exp122|Golgicide-A 4μM R03 exp122]]|1.92|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
No correlation found to any other genes in chemogenomics.
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 13/726
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|1/28|
|blood|0/28|
|bone|2/25|
|breast|0/33|
|central nervous system|0/56|
|cervix|1/4|
|colorectal|0/17|
|esophagus|0/13|
|fibroblast|0/1|
|gastric|0/15|
|kidney|0/21|
|liver|0/20|
|lung|2/75|
|lymphocyte|0/14|
|ovary|0/26|
|pancreas|0/24|
|peripheral nervous system|0/16|
|plasma cell|2/15|
|prostate|0/1|
|skin|0/24|
|soft tissue|1/7|
|thyroid|0/2|
|upper aerodigestive|0/22|
|urinary tract|1/29|
|uterus|0/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 246
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 6.42 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='ERCC3 Expression in NALM6 Cells: 6.42'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>