GNAI3 [The Human Chemical-Genetic Interaction Map Project]

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======= GNAI3 =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: GNAI3
  * **<color #00a2e8>Official Name</color>**: G protein subunit alpha i3
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=2773|2773]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/P08754|P08754]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=GNAI3&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20GNAI3|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/139370|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**:  Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling pathways. G proteins are composed of 3 units: alpha, beta and gamma. This gene encodes an alpha subunit and belongs to the G-alpha family. Mutation in this gene, resulting in a gly40-to-arg substitution, is associated with auriculocondylar syndrome, and shown to affect downstream targets in the G protein-coupled endothelin receptor pathway. [provided by RefSeq, Jun 2012]. 
  * **<color #00a2e8>UniProt Summary</color>**:  Heterotrimeric guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs) in numerous signaling cascades. The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state. Signaling by an activated GPCR promotes GDP release and GTP binding. The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal. Both GDP release and GTP hydrolysis are modulated by numerous regulatory proteins (PubMed:8774883, PubMed:18434541, PubMed:19478087). Signaling is mediated via effector proteins, such as adenylate cyclase. Inhibits adenylate cyclase activity, leading to decreased intracellular cAMP levels (PubMed:19478087). Stimulates the activity of receptor-regulated K(+) channels (PubMed:2535845). The active GTP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. May play a role in cell division (PubMed:17635935). {ECO:0000269|PubMed:17635935, ECO:0000269|PubMed:18434541, ECO:0000269|PubMed:2535845, ECO:0000269|PubMed:8774883}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|Arf|
|G-alpha|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|zymogen granule|
|G protein-coupled serotonin receptor binding|
|positive regulation of NAD(P)H oxidase activity|
|regulation of NAD(P)H oxidase activity|
|GTPase activating protein binding|
|negative regulation of adenylate cyclase activity|
|positive regulation of superoxide anion generation|
|G-protein beta/gamma-subunit complex binding|
|negative regulation of cyclase activity|
|negative regulation of lyase activity|
|regulation of superoxide anion generation|
|GTP metabolic process|
|regulation of adenylate cyclase activity|
|heterotrimeric G-protein complex|
|positive regulation of vascular smooth muscle cell proliferation|
|regulation of superoxide metabolic process|
|dopamine receptor signaling pathway|
|guanosine-containing compound metabolic process|
|regulation of cyclase activity|
|regulation of lyase activity|
|regulation of vascular smooth muscle cell proliferation|
|positive regulation of oxidoreductase activity|
|purine ribonucleoside triphosphate metabolic process|
|purine ribonucleoside metabolic process|
|purine nucleoside metabolic process|
|ribonucleoside triphosphate metabolic process|
|G protein-coupled receptor binding|
|positive regulation of macroautophagy|
|purine nucleoside triphosphate metabolic process|
|vesicle fusion|
|GDP binding|
|ribonucleoside metabolic process|
|organelle membrane fusion|
|adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway|
|positive regulation of smooth muscle cell proliferation|
|cellular response to dopamine|
|response to dopamine|
|signal transduction involved in cellular response to ammonium ion|
|nucleoside triphosphate metabolic process|
|regulation of oxidoreductase activity|
|positive regulation of reactive oxygen species metabolic process|
|organelle fusion|
|cellular response to catecholamine stimulus|
|cellular response to monoamine stimulus|
|response to monoamine|
|response to catecholamine|
|nucleoside metabolic process|
|positive regulation of autophagy|
|cellular response to ammonium ion|
|membrane fusion|
|regulation of smooth muscle cell proliferation|
|glycosyl compound metabolic process|
|midbody|
|regulation of macroautophagy|
|regulation of reactive oxygen species metabolic process|
|response to ammonium ion|
|adenylate cyclase-modulating G protein-coupled receptor signaling pathway|
|membrane raft|
|negative regulation of apoptotic signaling pathway|
|protein folding|
|protein domain specific binding|
|G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger|
|lysosomal membrane|
|vesicle organization|
|purine ribonucleotide metabolic process|
|ribonucleotide metabolic process|
|GTPase activity|
|purine nucleotide metabolic process|
|regulation of autophagy|
|ribose phosphate metabolic process|
|positive regulation of cellular catabolic process|
|purine-containing compound metabolic process|
|GTP binding|
|regulation of apoptotic signaling pathway|
|cellular response to drug|
|positive regulation of catabolic process|
|nucleotide metabolic process|
|nucleoside phosphate metabolic process|
|centrosome|
|cell division|
|nucleobase-containing small molecule metabolic process|
|cellular response to organic cyclic compound|
|cellular response to organonitrogen compound|
|Golgi membrane|
|cellular response to nitrogen compound|
|brain development|
|head development|
|negative regulation of catalytic activity|
|regulation of cellular catabolic process|
|membrane organization|
|nucleolus|
|organophosphate metabolic process|
|negative regulation of apoptotic process|
|negative regulation of programmed cell death|
|positive regulation of cell population proliferation|
|response to organic cyclic compound|
|endoplasmic reticulum membrane|
|central nervous system development|
|negative regulation of cell death|
|regulation of catabolic process|
|response to organonitrogen compound|
|response to drug|
|carbohydrate derivative metabolic process|
|cellular response to oxygen-containing compound|
|response to nitrogen compound|
|negative regulation of molecular function|
|cellular response to endogenous stimulus|
|negative regulation of signal transduction|
|G protein-coupled receptor signaling pathway|
|cell cycle|
|negative regulation of cell communication|
|negative regulation of signaling|
|positive regulation of catalytic activity|
|response to endogenous stimulus|
|regulation of apoptotic process|
|response to oxygen-containing compound|
|regulation of programmed cell death|
|regulation of cell population proliferation|
|negative regulation of response to stimulus|
|regulation of cell death|
|small molecule metabolic process|
|positive regulation of molecular function|
|vesicle-mediated transport|
|membrane|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp50|Nicotinamide 2000μM R01 exp50]]|-1.82|
|[[:results:exp10|CCCP 0.1μM R00 exp10]]|1.71|
|[[:results:exp225|Celastrol 0.12μM R05 exp225]]|1.74|
|[[:results:exp16|DABN 2μM R00 exp16]]|1.87|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:r:rrm1|RRM1]]|0.459|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 0/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|0/28|
|blood|0/28|
|bone|0/26|
|breast|0/33|
|central nervous system|0/56|
|cervix|0/4|
|colorectal|0/17|
|esophagus|0/13|
|fibroblast|0/1|
|gastric|0/16|
|kidney|0/21|
|liver|0/20|
|lung|0/75|
|lymphocyte|0/16|
|ovary|0/26|
|pancreas|0/24|
|peripheral nervous system|0/16|
|plasma cell|0/15|
|prostate|0/1|
|skin|0/24|
|soft tissue|0/9|
|thyroid|0/2|
|upper aerodigestive|0/22|
|urinary tract|0/29|
|uterus|0/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 9951
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 6.96 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='GNAI3 Expression in NALM6 Cells: 6.96'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>