GOT1 [The Human Chemical-Genetic Interaction Map Project]

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======= GOT1 =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: GOT1
  * **<color #00a2e8>Official Name</color>**: glutamic-oxaloacetic transaminase 1
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=2805|2805]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/P17174|P17174]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=GOT1&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20GOT1|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/138180|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**:  Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively.  GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. [provided by RefSeq, Jul 2008]. 
  * **<color #00a2e8>UniProt Summary</color>**:  Biosynthesis of L-glutamate from L-aspartate or L- cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H(2)S via the action of 3- mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain. {ECO:0000269|PubMed:16039064}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|Aminotran 1 2|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|L-cysteine:2-oxoglutarate aminotransferase activity|
|glutamate catabolic process to aspartate|
|response to transition metal nanoparticle|
|phosphatidylserine decarboxylase activity|
|glutamate catabolic process to 2-oxoglutarate|
|aspartate biosynthetic process|
|fumarate metabolic process|
|L-aspartate:2-oxoglutarate aminotransferase activity|
|aspartate catabolic process|
|glycerol biosynthetic process|
|negative regulation of mitochondrial depolarization|
|alditol biosynthetic process|
|negative regulation of membrane depolarization|
|transdifferentiation|
|aspartate metabolic process|
|glutamate catabolic process|
|carboxylic acid binding|
|oxaloacetate metabolic process|
|negative regulation of collagen biosynthetic process|
|negative regulation of collagen metabolic process|
|negative regulation of cytosolic calcium ion concentration|
|dicarboxylic acid biosynthetic process|
|fatty acid homeostasis|
|2-oxoglutarate metabolic process|
|dicarboxylic acid catabolic process|
|glycerol metabolic process|
|regulation of mitochondrial depolarization|
|aspartate family amino acid catabolic process|
|aspartate family amino acid biosynthetic process|
|alditol metabolic process|
|glutamine family amino acid catabolic process|
|response to immobilization stress|
|positive regulation of cellular response to transforming growth factor beta stimulus|
|positive regulation of transforming growth factor beta receptor signaling pathway|
|glutamate metabolic process|
|regulation of collagen biosynthetic process|
|regulation of collagen metabolic process|
|regulation of membrane depolarization|
|gluconeogenesis|
|hexose biosynthetic process|
|axon terminus|
|aspartate family amino acid metabolic process|
|monosaccharide biosynthetic process|
|pyridoxal phosphate binding|
|anion homeostasis|
|cellular carbohydrate biosynthetic process|
|polyol biosynthetic process|
|response to cadmium ion|
|alpha-amino acid biosynthetic process|
|regulation of mitochondrial membrane potential|
|glutamine family amino acid metabolic process|
|cellular response to mechanical stimulus|
|cellular amino acid biosynthetic process|
|dicarboxylic acid metabolic process|
|positive regulation of transmembrane receptor protein serine/threonine kinase signaling pathway|
|alpha-amino acid catabolic process|
|polyol metabolic process|
|alcohol biosynthetic process|
|regulation of transforming growth factor beta receptor signaling pathway|
|glucose metabolic process|
|regulation of cellular response to transforming growth factor beta stimulus|
|cellular amino acid catabolic process|
|Notch signaling pathway|
|carbohydrate biosynthetic process|
|lipid homeostasis|
|response to glucocorticoid|
|hexose metabolic process|
|response to corticosteroid|
|cellular response to insulin stimulus|
|cellular carbohydrate metabolic process|
|response to carbohydrate|
|organic hydroxy compound biosynthetic process|
|monosaccharide metabolic process|
|alpha-amino acid metabolic process|
|response to mechanical stimulus|
|response to insulin|
|regulation of transmembrane receptor protein serine/threonine kinase signaling pathway|
|lysosome|
|cellular response to peptide hormone stimulus|
|organic acid catabolic process|
|carboxylic acid catabolic process|
|regulation of cellular response to growth factor stimulus|
|carboxylic acid biosynthetic process|
|organic acid biosynthetic process|
|cellular amino acid metabolic process|
|cellular response to abiotic stimulus|
|cellular response to environmental stimulus|
|alcohol metabolic process|
|cellular response to peptide|
|regulation of cytosolic calcium ion concentration|
|response to steroid hormone|
|cellular response to external stimulus|
|response to metal ion|
|response to peptide hormone|
|regulation of membrane potential|
|cellular calcium ion homeostasis|
|small molecule catabolic process|
|cofactor metabolic process|
|calcium ion homeostasis|
|organic hydroxy compound metabolic process|
|cellular divalent inorganic cation homeostasis|
|response to peptide|
|carbohydrate metabolic process|
|divalent inorganic cation homeostasis|
|response to inorganic substance|
|cellular metal ion homeostasis|
|small molecule biosynthetic process|
|cellular response to organonitrogen compound|
|cellular response to hormone stimulus|
|metal ion homeostasis|
|cellular cation homeostasis|
|cellular ion homeostasis|
|cellular response to nitrogen compound|
|cation homeostasis|
|inorganic ion homeostasis|
|cellular chemical homeostasis|
|ion homeostasis|
|response to lipid|
|cellular homeostasis|
|response to hormone|
|carboxylic acid metabolic process|
|response to organic cyclic compound|
|response to organonitrogen compound|
|oxoacid metabolic process|
|organic acid metabolic process|
|cellular response to oxygen-containing compound|
|organonitrogen compound catabolic process|
|response to nitrogen compound|
|chemical homeostasis|
|response to abiotic stimulus|
|cellular response to endogenous stimulus|
|organonitrogen compound biosynthetic process|
|response to endogenous stimulus|
|negative regulation of biosynthetic process|
|response to oxygen-containing compound|
|homeostatic process|
|positive regulation of signal transduction|
|small molecule metabolic process|
|organic substance catabolic process|
|cellular catabolic process|
|positive regulation of cell communication|
|positive regulation of signaling|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp380|NMS-873 0.07μM R07 exp380]]|-5.76|
|[[:results:exp241|QNZ 0.01μM R05 exp241]]|-5.26|
|[[:results:exp212|Phenformin 20μM R05 exp212]]|-4.75|
|[[:results:exp274|Citral 50μM R06 exp274]]|-4.61|
|[[:results:exp143|Phenformin 20μM R03 exp143]]|-4.58|
|[[:results:exp431|Rotenone 0.07μM R08 exp431]]|-4.52|
|[[:results:exp13|Chloramphenicol 20μM R00 exp13]]|-4.37|
|[[:results:exp439|QNZ 0.01μM R08 exp439]]|-4.36|
|[[:results:exp492|iCRT14 30μM R08 exp492]]|-3.77|
|[[:results:exp400|Senexin-A 25μM R07 exp400]]|-3.7|
|[[:results:exp48|Mubritinib 0.2μM R01 exp48]]|-3.54|
|[[:results:exp209|Deguelin 0.15μM R05 exp209]]|-3.44|
|[[:results:exp248|UM0131023 0.05μM R05 exp248]]|-3.41|
|[[:results:exp342|Calcium Ionophore 0.4μM R07 exp342]]|-3.34|
|[[:results:exp65|Mubritinib 0.2μM R02 exp65]]|-3.34|
|[[:results:exp534|Trientine 500μM R08 exp534]]|-3.33|
|[[:results:exp465|Cannabidiol 13μM R08 exp465]]|-2.99|
|[[:results:exp447|Amiloride 100μM R08 exp447]]|-2.83|
|[[:results:exp519|RS-1 10μM R08 exp519]]|-2.68|
|[[:results:exp189|Temozolomide 200μM R04 exp189]]|-2.56|
|[[:results:exp85|UM0129480 7μM R02 exp85]]|-2.21|
|[[:results:exp487|Hinokiflavone 12μM R08 exp487]]|-2.02|
|[[:results:exp466|Cannabidiol 20μM R08 exp466]]|-1.95|
|[[:results:exp532|TIC10 10μM R08 exp532]]|-1.95|
|[[:results:exp531|THZ1 0.06μM R08 exp531]]|-1.94|
|[[:results:exp445|∆-9-Tetrahydrocannabinol 30μM R08 exp445]]|-1.92|
|[[:results:exp344|Chlorpromazine 10μM R07 exp344]]|-1.83|
|[[:results:exp160|Ribavirin 10 to 15μM on day4 R04 exp160]]|-1.77|
|[[:results:exp350|Deferoxamine 11μM R07 exp350]]|-1.73|
|[[:results:exp54|Taxol 0.002μM R01 exp54]]|1.73|
|[[:results:exp165|RO-3306 3 to 4μM on day4 R04 exp165]]|1.76|
|[[:results:exp11|CCCP 1μM R00 exp11]]|1.78|
|[[:results:exp229|Dimethyloxaloylglycine 100μM R05 exp229]]|1.8|
|[[:results:exp425|Beta-Estradiol 0.55μM R07 exp425]]|1.83|
|[[:results:exp216|Erlotinib 10μM R05 exp216]]|1.97|
|[[:results:exp133|MKC9989 10μM R03 exp133]]|2.27|
|[[:results:exp51|Nifuroxazide 1μM R01 exp51]]|2.28|
|[[:results:exp233|EPZ-5676 30μM R05 exp233]]|2.45|
|[[:results:exp234|Ethanol 0.01 R05 exp234]]|2.79|
|[[:results:exp210|LB-100 2μM R05 exp210]]|3.41|
|[[:results:exp102|Nifuroxazide 5μM R03 exp102]]|3.5|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:h:hk1|HK1]]|0.501|
|[[:human genes:m:mlf2|MLF2]]|0.462|
|[[:human genes:p:pfkp|PFKP]]|0.439|
|[[:human genes:b:bsg|BSG]]|0.411|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 0/726
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|0/28|
|blood|0/28|
|bone|0/25|
|breast|0/33|
|central nervous system|0/56|
|cervix|0/4|
|colorectal|0/17|
|esophagus|0/13|
|fibroblast|0/1|
|gastric|0/15|
|kidney|0/21|
|liver|0/20|
|lung|0/75|
|lymphocyte|0/14|
|ovary|0/26|
|pancreas|0/24|
|peripheral nervous system|0/16|
|plasma cell|0/15|
|prostate|0/1|
|skin|0/24|
|soft tissue|0/7|
|thyroid|0/2|
|upper aerodigestive|0/22|
|urinary tract|0/29|
|uterus|0/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 1698
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 4.15 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='GOT1 Expression in NALM6 Cells: 4.15'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>