HUS1 [The Human Chemical-Genetic Interaction Map Project]

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======= HUS1 =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: HUS1
  * **<color #00a2e8>Official Name</color>**: HUS1 checkpoint clamp component
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=3364|3364]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/O60921|O60921]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=HUS1&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20HUS1|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/603760|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**: N/A
  * **<color #00a2e8>UniProt Summary</color>**:  Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase. {ECO:0000269|PubMed:21659603}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|Hus1|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|meiotic DNA integrity checkpoint|
|checkpoint clamp complex|
|meiotic cell cycle checkpoint|
|mitotic DNA replication checkpoint|
|intra-S DNA damage checkpoint|
|DNA replication checkpoint|
|mitotic G2/M transition checkpoint|
|negative regulation of DNA replication|
|site of double-strand break|
|cellular response to ionizing radiation|
|negative regulation of G2/M transition of mitotic cell cycle|
|mitotic DNA damage checkpoint|
|double-strand break repair via homologous recombination|
|telomere maintenance|
|recombinational repair|
|negative regulation of cell cycle G2/M phase transition|
|telomere organization|
|mitotic DNA integrity checkpoint|
|regulation of DNA replication|
|nucleotide-excision repair|
|DNA damage checkpoint|
|response to UV|
|DNA integrity checkpoint|
|response to ionizing radiation|
|mitotic cell cycle checkpoint|
|meiotic cell cycle process|
|cellular response to radiation|
|double-strand break repair|
|regulation of signal transduction by p53 class mediator|
|cell cycle checkpoint|
|regulation of G2/M transition of mitotic cell cycle|
|regulation of cell cycle G2/M phase transition|
|negative regulation of mitotic cell cycle phase transition|
|DNA replication|
|DNA recombination|
|meiotic cell cycle|
|negative regulation of cell cycle phase transition|
|response to light stimulus|
|negative regulation of mitotic cell cycle|
|cellular response to environmental stimulus|
|cellular response to abiotic stimulus|
|negative regulation of cell cycle process|
|anatomical structure homeostasis|
|regulation of mitotic cell cycle phase transition|
|response to radiation|
|regulation of cell cycle phase transition|
|DNA repair|
|negative regulation of cell cycle|
|mitotic cell cycle process|
|regulation of mitotic cell cycle|
|embryo development ending in birth or egg hatching|
|mitotic cell cycle|
|DNA metabolic process|
|regulation of cell cycle process|
|cellular response to DNA damage stimulus|
|nucleolus|
|protein phosphorylation|
|embryo development|
|cell cycle process|
|chromosome organization|
|response to abiotic stimulus|
|regulation of cell cycle|
|phosphorylation|
|cell cycle|
|negative regulation of cellular macromolecule biosynthetic process|
|reproductive process|
|reproduction|
|regulation of protein phosphorylation|
|negative regulation of macromolecule biosynthetic process|
|negative regulation of cellular biosynthetic process|
|negative regulation of biosynthetic process|
|regulation of phosphorylation|
|homeostatic process|
|cellular response to stress|
|cellular macromolecule biosynthetic process|
|macromolecule biosynthetic process|
|regulation of phosphate metabolic process|
|regulation of phosphorus metabolic process|
|regulation of intracellular signal transduction|
|regulation of protein modification process|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='default' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp391|Pomalidomide 20μM R07 exp391]]|-1.96|
|[[:results:exp334|All-trans-Retinoic-Acid 40μM R07 exp334]]|-1.95|
|[[:results:exp78|Pterostilbene 16μM R02 exp78]]|-1.9|
|[[:results:exp512|Olaparib 4μM R08 exp512]]|-1.89|
|[[:results:exp354|Diepoxybutane 3μM R07 exp354]]|-1.87|
|[[:results:exp345|Cidofovir 10μM R07 exp345]]|-1.86|
|[[:results:exp282|Fluvastatin 2.2μM R06 exp282]]|-1.79|
|[[:results:exp335|Aminopterin 0.005μM R07 exp335]]|-1.73|
|[[:results:exp329|Hydroxyurea 100μM R07 exp329]]|-1.72|
|[[:results:exp431|Rotenone 0.07μM R08 exp431]]|-1.7|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
No correlation found to any other genes in chemogenomics.
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 44/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|0/28|
|blood|7/28|
|bone|2/26|
|breast|3/33|
|central nervous system|2/56|
|cervix|0/4|
|colorectal|0/17|
|esophagus|1/13|
|fibroblast|0/1|
|gastric|1/16|
|kidney|2/21|
|liver|2/20|
|lung|5/75|
|lymphocyte|1/16|
|ovary|1/26|
|pancreas|1/24|
|peripheral nervous system|1/16|
|plasma cell|1/15|
|prostate|0/1|
|skin|0/24|
|soft tissue|0/9|
|thyroid|0/2|
|upper aerodigestive|1/22|
|urinary tract|2/29|
|uterus|0/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 833
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 4.83 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='HUS1 Expression in NALM6 Cells: 4.83'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>