IRF3 [The Human Chemical-Genetic Interaction Map Project]

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======= IRF3 =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: IRF3
  * **<color #00a2e8>Official Name</color>**: interferon regulatory factor 3
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=3661|3661]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/Q14653|Q14653]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=IRF3&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20IRF3|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/603734|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**:  This gene encodes a member of the interferon regulatory transcription factor (IRF) family. The encoded protein is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011]. 
  * **<color #00a2e8>UniProt Summary</color>**:  Key transcriptional regulator of type I interferon (IFN)-dependent immune responses which plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction. Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, is phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|IRF|
|IRF-3|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|macrophage apoptotic process|
|type I interferon biosynthetic process|
|MDA-5 signaling pathway|
|myeloid cell apoptotic process|
|inflammatory cell apoptotic process|
|cytoplasmic pattern recognition receptor signaling pathway in response to virus|
|type I interferon production|
|positive regulation of type I interferon-mediated signaling pathway|
|cytokine biosynthetic process|
|cytokine metabolic process|
|cellular response to exogenous dsRNA|
|multi-organism cellular process|
|cellular response to dsRNA|
|positive regulation of interferon-alpha production|
|leukocyte apoptotic process|
|programmed necrotic cell death|
|regulation of interferon-alpha production|
|TRIF-dependent toll-like receptor signaling pathway|
|necrotic cell death|
|positive regulation of interferon-beta production|
|MyD88-independent toll-like receptor signaling pathway|
|cellular response to virus|
|lipopolysaccharide-mediated signaling pathway|
|regulation of type I interferon-mediated signaling pathway|
|cytoplasmic pattern recognition receptor signaling pathway|
|response to exogenous dsRNA|
|negative regulation of type I interferon production|
|regulation of interferon-beta production|
|positive regulation of cytokine-mediated signaling pathway|
|response to dsRNA|
|positive regulation of response to cytokine stimulus|
|cellular response to type I interferon|
|type I interferon signaling pathway|
|response to type I interferon|
|interferon-gamma-mediated signaling pathway|
|positive regulation of type I interferon production|
|transcription coregulator activity|
|toll-like receptor signaling pathway|
|regulation of type I interferon production|
|pattern recognition receptor signaling pathway|
|positive regulation of cytokine secretion|
|cytokine production|
|cellular response to interferon-gamma|
|regulation of cytokine-mediated signaling pathway|
|intracellular receptor signaling pathway|
|regulation of response to cytokine stimulus|
|response to interferon-gamma|
|cellular response to lipopolysaccharide|
|positive regulation of I-kappaB kinase/NF-kappaB signaling|
|cellular response to molecule of bacterial origin|
|defense response to virus|
|regulation of cytokine secretion|
|cellular response to biotic stimulus|
|innate immune response-activating signal transduction|
|regulation of I-kappaB kinase/NF-kappaB signaling|
|protein domain specific binding|
|DNA-binding transcription repressor activity, RNA polymerase II-specific|
|activation of innate immune response|
|positive regulation of protein secretion|
|negative regulation of cytokine production|
|response to virus|
|positive regulation of peptide secretion|
|response to lipopolysaccharide|
|response to molecule of bacterial origin|
|regulation of inflammatory response|
|positive regulation of innate immune response|
|positive regulation of response to biotic stimulus|
|positive regulation of secretion by cell|
|sequence-specific DNA binding|
|positive regulation of protein transport|
|positive regulation of secretion|
|DNA-binding transcription activator activity, RNA polymerase II-specific|
|positive regulation of cytokine production|
|regulation of innate immune response|
|positive regulation of establishment of protein localization|
|regulation of protein secretion|
|positive regulation of defense response|
|transcription by RNA polymerase II|
|regulation of peptide secretion|
|RNA polymerase II proximal promoter sequence-specific DNA binding|
|positive regulation of multi-organism process|
|cellular response to lipid|
|regulation of response to biotic stimulus|
|cellular response to organic cyclic compound|
|immune response-activating signal transduction|
|immune response-regulating signaling pathway|
|positive regulation of response to external stimulus|
|activation of immune response|
|transcription, DNA-templated|
|nucleic acid-templated transcription|
|RNA biosynthetic process|
|cellular response to nitrogen compound|
|cytokine-mediated signaling pathway|
|response to bacterium|
|regulation of cytokine production|
|viral process|
|regulation of protein transport|
|regulation of peptide transport|
|regulation of establishment of protein localization|
|regulation of secretion by cell|
|regulation of defense response|
|innate immune response|
|regulation of multi-organism process|
|cellular response to DNA damage stimulus|
|symbiotic process|
|regulation of secretion|
|interspecies interaction between organisms|
|response to lipid|
|negative regulation of transcription by RNA polymerase II|
|positive regulation of immune response|
|protein homodimerization activity|
|response to organic cyclic compound|
|apoptotic process|
|defense response to other organism|
|positive regulation of transport|
|cellular response to cytokine stimulus|
|positive regulation of intracellular signal transduction|
|regulation of protein localization|
|programmed cell death|
|cellular response to oxygen-containing compound|
|identical protein binding|
|response to nitrogen compound|
|immune effector process|
|cell death|
|regulation of response to external stimulus|
|nucleobase-containing compound biosynthetic process|
|response to cytokine|
|regulation of immune response|
|positive regulation of immune system process|
|heterocycle biosynthetic process|
|aromatic compound biosynthetic process|
|negative regulation of transcription, DNA-templated|
|negative regulation of multicellular organismal process|
|positive regulation of transcription by RNA polymerase II|
|negative regulation of nucleic acid-templated transcription|
|negative regulation of RNA biosynthetic process|
|response to other organism|
|organic cyclic compound biosynthetic process|
|response to external biotic stimulus|
|response to biotic stimulus|
|negative regulation of RNA metabolic process|
|defense response|
|negative regulation of cellular macromolecule biosynthetic process|
|negative regulation of nucleobase-containing compound metabolic process|
|DNA binding|
|negative regulation of macromolecule biosynthetic process|
|regulation of response to stress|
|negative regulation of cellular biosynthetic process|
|positive regulation of transcription, DNA-templated|
|negative regulation of biosynthetic process|
|response to oxygen-containing compound|
|DNA-binding transcription factor activity, RNA polymerase II-specific|
|cellular nitrogen compound biosynthetic process|
|positive regulation of nucleic acid-templated transcription|
|positive regulation of RNA biosynthetic process|
|positive regulation of signal transduction|
|regulation of immune system process|
|RNA metabolic process|
|intracellular signal transduction|
|cellular response to stress|
|cellular macromolecule biosynthetic process|
|negative regulation of gene expression|
|positive regulation of RNA metabolic process|
|positive regulation of multicellular organismal process|
|macromolecule biosynthetic process|
|positive regulation of cell communication|
|positive regulation of signaling|
|regulation of intracellular signal transduction|
|regulation of transport|
|immune response|
|positive regulation of nucleobase-containing compound metabolic process|
|positive regulation of macromolecule biosynthetic process|
|positive regulation of cellular biosynthetic process|
|positive regulation of gene expression|
|gene expression|
|positive regulation of biosynthetic process|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='default' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp350|Deferoxamine 11μM R07 exp350]]|2.12|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
No correlation found to any other genes in chemogenomics.
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 0/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|0/28|
|blood|0/28|
|bone|0/26|
|breast|0/33|
|central nervous system|0/56|
|cervix|0/4|
|colorectal|0/17|
|esophagus|0/13|
|fibroblast|0/1|
|gastric|0/16|
|kidney|0/21|
|liver|0/20|
|lung|0/75|
|lymphocyte|0/16|
|ovary|0/26|
|pancreas|0/24|
|peripheral nervous system|0/16|
|plasma cell|0/15|
|prostate|0/1|
|skin|0/24|
|soft tissue|0/9|
|thyroid|0/2|
|upper aerodigestive|0/22|
|urinary tract|0/29|
|uterus|0/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 7280
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 6.02 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='IRF3 Expression in NALM6 Cells: 6.02'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>