MANF [The Human Chemical-Genetic Interaction Map Project]

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======= MANF =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: MANF
  * **<color #00a2e8>Official Name</color>**: mesencephalic astrocyte derived neurotrophic factor
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=7873|7873]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/P55145|P55145]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=MANF&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20MANF|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/601916|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**:  The protein encoded by this gene is localized in the endoplasmic reticulum (ER) and golgi, and is also secreted. Reducing expression of this gene increases susceptibility to ER stress-induced death and results in cell proliferation. Activity of this protein is important in promoting the survival of dopaminergic neurons. The presence of polymorphisms in the N-terminal arginine-rich region, including a specific mutation that changes an ATG start codon to AGG, have been reported in a variety of solid tumors; however, these polymorphisms were later shown to exist in normal tissues and are thus no longer thought to be tumor-related. [provided by RefSeq, Apr 2014]. 
  * **<color #00a2e8>UniProt Summary</color>**:  Selectively promotes the survival of dopaminergic neurons of the ventral mid-brain. Modulates GABAergic transmission to the dopaminergic neurons of the substantia nigra. Enhances spontaneous, as well as evoked, GABAergic inhibitory postsynaptic currents in dopaminergic neurons (By similarity). Inhibits cell proliferation and endoplasmic reticulum (ER) stress-induced cell death. {ECO:0000250, ECO:0000269|PubMed:12794311, ECO:0000269|PubMed:18561914}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|Armet|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|sulfatide binding|
|sarcoplasmic reticulum lumen|
|dopaminergic neuron differentiation|
|regulation of response to endoplasmic reticulum stress|
|platelet degranulation|
|response to unfolded protein|
|growth factor activity|
|response to topologically incorrect protein|
|endoplasmic reticulum lumen|
|neuron projection development|
|regulated exocytosis|
|regulation of cellular response to stress|
|exocytosis|
|neuron development|
|secretion by cell|
|endoplasmic reticulum|
|neuron differentiation|
|export from cell|
|plasma membrane bounded cell projection organization|
|secretion|
|cell projection organization|
|RNA binding|
|regulation of response to stress|
|generation of neurons|
|extracellular space|
|neurogenesis|
|cell development|
|extracellular region|
|vesicle-mediated transport|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp420|Tunicamycin 0.04 to 0.125μM  on day4 R07 exp420]]|-2.88|
|[[:results:exp122|Golgicide-A 4μM R03 exp122]]|-2.84|
|[[:results:exp155|UNC1999 2μM R03 exp155]]|-2.29|
|[[:results:exp407|Thapsigargin 0.005μM R07 exp407]]|-2.14|
|[[:results:exp498|Lead acetate 2000μM R08 exp498 no dilution day6]]|-1.87|
|[[:results:exp347|Cyclosporin-A 0.8μM R07 exp347]]|-1.84|
|[[:results:exp307|Rapamycin 2μM plus Cyclosporin-A 3μM R07 exp307]]|-1.84|
|[[:results:exp227|Cryptotanshinone 12μM R05 exp227]]|-1.8|
|[[:results:exp419|Tunicamycin 0.04 to 0.075μM  on day4 R07 exp419]]|-1.78|
|[[:results:exp269|Bisphenol A 100μM R06 exp269]]|-1.75|
|[[:results:exp334|All-trans-Retinoic-Acid 40μM R07 exp334]]|-1.72|
|[[:results:exp399|Salubrinal 20μM R07 exp399]]|-1.71|
|[[:results:exp489|Hippuristanol 0.12μM R08 exp489 no dilution day6]]|1.78|
|[[:results:exp79|Q15 2.7μM R02 exp79]]|2.11|
|[[:results:exp515|PU-H71 1μM R08 exp515]]|2.14|
|[[:results:exp2|5-Fluorouracil 20μM R00 exp2]]|2.28|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:d:dnajc3|DNAJC3]]|0.73|
|[[:human genes:u:ube2j1|UBE2J1]]|0.619|
|[[:human genes:d:derl2|DERL2]]|0.602|
|[[:human genes:u:ube2g2|UBE2G2]]|0.554|
|[[:human genes:a:alg5|ALG5]]|0.55|
|[[:human genes:s:sec63|SEC63]]|0.541|
|[[:human genes:a:alg9|ALG9]]|0.538|
|[[:human genes:d:derl1|DERL1]]|0.528|
|[[:human genes:t:tmed10|TMED10]]|0.524|
|[[:human genes:s:sec62|SEC62]]|0.513|
|[[:human genes:d:dnajb11|DNAJB11]]|0.506|
|[[:human genes:s:slc33a1|SLC33A1]]|0.506|
|[[:human genes:i:irf2bp2|IRF2BP2]]|0.497|
|[[:human genes:a:aup1|AUP1]]|0.484|
|[[:human genes:u:ufm1|UFM1]]|0.47|
|[[:human genes:s:sec61b|SEC61B]]|0.46|
|[[:human genes:a:alg6|ALG6]]|0.46|
|[[:human genes:h:herpud1|HERPUD1]]|0.456|
|[[:human genes:v:vmp1|VMP1]]|0.453|
|[[:human genes:h:hars|HARS]]|0.45|
|[[:human genes:c:c1orf27|C1orf27]]|0.449|
|[[:human genes:x:xpo6|XPO6]]|0.448|
|[[:human genes:e:eny2|ENY2]]|0.442|
|[[:human genes:d:dpm1|DPM1]]|0.438|
|[[:human genes:s:slc35b1|SLC35B1]]|0.433|
|[[:human genes:a:alg8|ALG8]]|0.429|
|[[:human genes:u:usp22|USP22]]|0.428|
|[[:human genes:u:ufc1|UFC1]]|0.424|
|[[:human genes:d:ddx5|DDX5]]|0.422|
|[[:human genes:d:dpm3|DPM3]]|0.421|
|[[:human genes:s:smg9|SMG9]]|0.419|
|[[:human genes:u:uba5|UBA5]]|0.417|
|[[:human genes:m:mpdu1|MPDU1]]|0.416|
|[[:human genes:a:alg12|ALG12]]|0.416|
|[[:human genes:o:opa1|OPA1]]|0.412|
|[[:human genes:d:dpagt1|DPAGT1]]|0.411|
|[[:human genes:t:tmem41b|TMEM41B]]|0.403|
|[[:human genes:s:stt3a|STT3A]]|0.402|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 22/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|0/28|
|blood|2/28|
|bone|2/26|
|breast|0/33|
|central nervous system|0/56|
|cervix|0/4|
|colorectal|1/17|
|esophagus|0/13|
|fibroblast|0/1|
|gastric|0/16|
|kidney|0/21|
|liver|0/20|
|lung|2/75|
|lymphocyte|0/16|
|ovary|0/26|
|pancreas|0/24|
|peripheral nervous system|1/16|
|plasma cell|8/15|
|prostate|0/1|
|skin|1/24|
|soft tissue|0/9|
|thyroid|1/2|
|upper aerodigestive|1/22|
|urinary tract|0/29|
|uterus|0/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 1779
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 6.3 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='MANF Expression in NALM6 Cells: 6.3'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>