MCM2 [The Human Chemical-Genetic Interaction Map Project]

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======= MCM2 =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: MCM2
  * **<color #00a2e8>Official Name</color>**: minichromosome maintenance complex component 2
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=4171|4171]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/P49736|P49736]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=MCM2&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20MCM2|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/116945|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**:  The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq, Oct 2012]. 
  * **<color #00a2e8>UniProt Summary</color>**:  Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division. Plays a role in terminally differentiated hair cells development of the cochlea and induces cells apoptosis. {ECO:0000269|PubMed:26196677, ECO:0000269|PubMed:8175912}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|MCM2 N|
|AAA 5|
|Mg chelatase|
|MCM|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|negative regulation of DNA helicase activity|
|negative regulation of DNA duplex unwinding|
|mitotic DNA replication initiation|
|cell cycle DNA replication initiation|
|regulation of DNA helicase activity|
|nuclear cell cycle DNA replication initiation|
|negative regulation of helicase activity|
|regulation of DNA duplex unwinding|
|pre-replicative complex assembly involved in cell cycle DNA replication|
|pre-replicative complex assembly|
|pre-replicative complex assembly involved in nuclear cell cycle DNA replication|
|nuclear origin of replication recognition complex|
|mitotic DNA replication|
|double-strand break repair via break-induced replication|
|MCM complex|
|regulation of helicase activity|
|DNA unwinding involved in DNA replication|
|single-stranded DNA helicase activity|
|3-5 DNA helicase activity|
|negative regulation of ATPase activity|
|DNA replication origin binding|
|cellular response to interleukin-4|
|response to interleukin-4|
|DNA replication initiation|
|nuclear DNA replication|
|cell cycle DNA replication|
|cochlea development|
|regulation of ATPase activity|
|double-strand break repair via homologous recombination|
|recombinational repair|
|single-stranded DNA binding|
|nuclear chromosome, telomeric region|
|DNA duplex unwinding|
|chromatin|
|nucleosome assembly|
|G1/S transition of mitotic cell cycle|
|DNA geometric change|
|cell cycle G1/S phase transition|
|DNA-dependent DNA replication|
|histone binding|
|chromatin assembly|
|negative regulation of chromosome organization|
|microtubule cytoskeleton|
|chromatin assembly or disassembly|
|nucleosome organization|
|DNA packaging|
|double-strand break repair|
|inner ear development|
|protein-DNA complex assembly|
|DNA replication|
|ear development|
|DNA recombination|
|protein-DNA complex subunit organization|
|mitotic cell cycle phase transition|
|cell cycle phase transition|
|DNA conformation change|
|enzyme binding|
|regulation of chromosome organization|
|negative regulation of organelle organization|
|negative regulation of hydrolase activity|
|DNA repair|
|sensory organ development|
|mitotic cell cycle process|
|mitotic cell cycle|
|chromatin organization|
|negative regulation of cellular component organization|
|DNA metabolic process|
|cellular response to DNA damage stimulus|
|negative regulation of catalytic activity|
|cellular protein-containing complex assembly|
|nucleolus|
|apoptotic process|
|cell cycle process|
|cellular response to cytokine stimulus|
|programmed cell death|
|chromosome organization|
|cell death|
|response to cytokine|
|negative regulation of molecular function|
|regulation of hydrolase activity|
|regulation of organelle organization|
|cell cycle|
|DNA binding|
|ATP binding|
|protein-containing complex assembly|
|cellular response to stress|
|cellular macromolecule biosynthetic process|
|macromolecule biosynthetic process|
|protein-containing complex subunit organization|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='default' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp505|ML-792 0.2μM R08 exp505]]|-2.14|
|[[:results:exp520|Rucaparib 6.5μM R08 exp520]]|-2.07|
|[[:results:exp294|Nutlin-3A 1.6μM R06 exp294]]|-2.06|
|[[:results:exp531|THZ1 0.06μM R08 exp531]]|-1.9|
|[[:results:exp478|Doxorubicin 0.02μM R08 exp478]]|-1.79|
|[[:results:exp416|Tubacin 1.6μM R07 exp416]]|-1.73|
|[[:results:exp97|BI-6727 0.0125μM R03 exp97]]|-1.71|
|[[:results:exp537|WNT3A 44ng/ml R08 exp537]]|1.79|
|[[:results:exp243|S-trityl-L-cysteine 0.5μM R05 exp243]]|1.82|
|[[:results:exp438|NN-Diethyl-meta-toluamide 500μM R08 exp438]]|1.86|
|[[:results:exp187|proTAME 5μM R04 exp187]]|1.92|
|[[:results:exp528|TGF-beta1 44ng/ml R08 exp528]]|2.48|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
No correlation found to any other genes in chemogenomics.
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 717/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|1/1|
|909776.0|1/1|
|bile duct|27/28|
|blood|28/28|
|bone|26/26|
|breast|33/33|
|central nervous system|52/56|
|cervix|3/4|
|colorectal|17/17|
|esophagus|12/13|
|fibroblast|1/1|
|gastric|16/16|
|kidney|20/21|
|liver|19/20|
|lung|73/75|
|lymphocyte|16/16|
|ovary|26/26|
|pancreas|24/24|
|peripheral nervous system|15/16|
|plasma cell|15/15|
|prostate|1/1|
|skin|24/24|
|soft tissue|9/9|
|thyroid|2/2|
|upper aerodigestive|21/22|
|urinary tract|27/29|
|uterus|5/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 299
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 8.43 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='MCM2 Expression in NALM6 Cells: 8.43'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>