MUS81 [The Human Chemical-Genetic Interaction Map Project]

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======= MUS81 =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: MUS81
  * **<color #00a2e8>Official Name</color>**: MUS81 structure-specific endonuclease subunit
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=80198|80198]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/Q96NY9|Q96NY9]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=MUS81&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20MUS81|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/606591|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**:  This gene encodes a structure-specific endonuclease which belongs to the XPF/MUS81 endonuclease family and plays a critical role in the resolution of recombination intermediates during DNA repair after inter-strand cross-links, replication fork collapse, and DNA double-strand breaks. The encoded protein associates with one of two closely related essential meiotic endonuclease proteins (EME1 or EME2) to form a complex that processes DNA secondary structures. It contains an N-terminal DEAH helicase domain, an excision repair cross complementation group 4 (ERCC4) endonuclease domain, and two tandem C-terminal helix-hairpin-helix domains. Mice with a homozygous knockout of the orthologous gene have significant meiotic defects including the failure to repair a subset of DNA double strand breaks. [provided by RefSeq, Jun 2017]. 
  * **<color #00a2e8>UniProt Summary</color>**:  Interacts with EME1 and EME2 to form a DNA structure- specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks. {ECO:0000269|PubMed:11741546, ECO:0000269|PubMed:12374758, ECO:0000269|PubMed:12686547, ECO:0000269|PubMed:12721304, ECO:0000269|PubMed:14617801, ECO:0000269|PubMed:15805243, ECO:0000269|PubMed:17289582, ECO:0000269|PubMed:19595721, ECO:0000269|PubMed:19596235}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|ERCC4|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|3-flap endonuclease activity|
|response to intra-S DNA damage checkpoint signaling|
|Holliday junction resolvase complex|
|response to DNA damage checkpoint signaling|
|response to cell cycle checkpoint signaling|
|response to DNA integrity checkpoint signaling|
|crossover junction endodeoxyribonuclease activity|
|double-strand break repair via break-induced replication|
|intra-S DNA damage checkpoint|
|resolution of meiotic recombination intermediates|
|meiotic chromosome separation|
|DNA catabolic process, endonucleolytic|
|DNA catabolic process|
|chromosome separation|
|reciprocal meiotic recombination|
|interstrand cross-link repair|
|homologous recombination|
|meiotic chromosome segregation|
|mitotic DNA damage checkpoint|
|double-strand break repair via homologous recombination|
|recombinational repair|
|mitotic DNA integrity checkpoint|
|meiosis I|
|meiosis I cell cycle process|
|DNA damage checkpoint|
|DNA integrity checkpoint|
|meiotic nuclear division|
|mitotic cell cycle checkpoint|
|meiotic cell cycle process|
|double-strand break repair|
|cell cycle checkpoint|
|cellular response to biotic stimulus|
|nuclear chromosome segregation|
|DNA recombination|
|meiotic cell cycle|
|chromosome segregation|
|nuclear division|
|nucleic acid phosphodiester bond hydrolysis|
|negative regulation of mitotic cell cycle|
|organelle fission|
|nucleobase-containing compound catabolic process|
|heterocycle catabolic process|
|cellular nitrogen compound catabolic process|
|aromatic compound catabolic process|
|organic cyclic compound catabolic process|
|DNA repair|
|negative regulation of cell cycle|
|mitotic cell cycle process|
|regulation of mitotic cell cycle|
|mitotic cell cycle|
|DNA metabolic process|
|cellular response to DNA damage stimulus|
|nucleolus|
|cellular macromolecule catabolic process|
|cell cycle process|
|macromolecule catabolic process|
|regulation of cell cycle|
|cellular response to endogenous stimulus|
|response to biotic stimulus|
|cell cycle|
|reproductive process|
|reproduction|
|DNA binding|
|response to endogenous stimulus|
|cellular response to stress|
|organic substance catabolic process|
|cellular catabolic process|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='default' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp520|Rucaparib 6.5μM R08 exp520]]|-3.23|
|[[:results:exp512|Olaparib 4μM R08 exp512]]|-2.33|
|[[:results:exp25|Oligomycin-A 2μM R00 exp25]]|-2.22|
|[[:results:exp1|5-Fluorouracil 2μM R00 exp1]]|-2|
|[[:results:exp29|Rapamycin 1μM R00 exp29]]|-1.93|
|[[:results:exp400|Senexin-A 25μM R07 exp400]]|-1.88|
|[[:results:exp14|Cycloheximide 0.02μM R00 exp14]]|-1.75|
|[[:results:exp278|CVT-10216 0.1μM R06 exp278]]|1.71|
|[[:results:exp280|Daidzin 10μM R06 exp280]]|1.98|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
No correlation found to any other genes in chemogenomics.
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 7/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|0/28|
|blood|1/28|
|bone|0/26|
|breast|1/33|
|central nervous system|0/56|
|cervix|0/4|
|colorectal|1/17|
|esophagus|0/13|
|fibroblast|0/1|
|gastric|0/16|
|kidney|0/21|
|liver|0/20|
|lung|0/75|
|lymphocyte|0/16|
|ovary|1/26|
|pancreas|0/24|
|peripheral nervous system|0/16|
|plasma cell|0/15|
|prostate|0/1|
|skin|1/24|
|soft tissue|0/9|
|thyroid|0/2|
|upper aerodigestive|0/22|
|urinary tract|1/29|
|uterus|0/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 6585
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 5.61 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='MUS81 Expression in NALM6 Cells: 5.61'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>