NAE1 [The Human Chemical-Genetic Interaction Map Project]

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======= NAE1 =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: N/AE1
  * **<color #00a2e8>Official Name</color>**: NEDD8 activating enzyme E1 subunit 1
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=8883|8883]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/Q13564|Q13564]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=NAE1&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20NAE1|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/603385|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**: N/A
  * **<color #00a2e8>UniProt Summary</color>**:  Regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Necessary for cell cycle progression through the S-M checkpoint. Overexpression of NAE1 causes apoptosis through deregulation of NEDD8 conjugation. {ECO:0000269|PubMed:10207026, ECO:0000269|PubMed:10722740, ECO:0000269|PubMed:12740388}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|ThiF|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|NEDD8 activating enzyme activity|
|mitotic DNA replication checkpoint|
|protein neddylation|
|DNA replication checkpoint|
|mitotic G2/M transition checkpoint|
|neuron apoptotic process|
|neuron death|
|negative regulation of G2/M transition of mitotic cell cycle|
|negative regulation of cell cycle G2/M phase transition|
|mitotic DNA integrity checkpoint|
|DNA integrity checkpoint|
|mitotic cell cycle checkpoint|
|cell cycle checkpoint|
|regulation of G2/M transition of mitotic cell cycle|
|regulation of neuron apoptotic process|
|regulation of cell cycle G2/M phase transition|
|negative regulation of mitotic cell cycle phase transition|
|negative regulation of cell cycle phase transition|
|ubiquitin protein ligase binding|
|negative regulation of mitotic cell cycle|
|regulation of neuron death|
|negative regulation of cell cycle process|
|post-translational protein modification|
|regulation of mitotic cell cycle phase transition|
|regulation of cell cycle phase transition|
|protein heterodimerization activity|
|negative regulation of cell cycle|
|protein-containing complex|
|mitotic cell cycle process|
|regulation of mitotic cell cycle|
|mitotic cell cycle|
|regulation of cell cycle process|
|protein modification by small protein conjugation|
|apoptotic process|
|protein modification by small protein conjugation or removal|
|cell cycle process|
|programmed cell death|
|cell death|
|regulation of cell cycle|
|cell cycle|
|regulation of apoptotic process|
|regulation of programmed cell death|
|regulation of cell death|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp113|1-Methyl-nicotinamide-chloride 1000μM R03 exp113]]|-2.52|
|[[:results:exp345|Cidofovir 10μM R07 exp345]]|-2.47|
|[[:results:exp341|BRD2 inhibitor II 20μM R07 exp341]]|-2.21|
|[[:results:exp143|Phenformin 20μM R03 exp143]]|-2.01|
|[[:results:exp27|Pimelic-diphenylamide-106 0.5μM R00 exp27]]|-1.94|
|[[:results:exp501|Methotrexate 0.01μM R08 exp501]]|-1.92|
|[[:results:exp220|BAY-598 4μM R05 exp220]]|-1.86|
|[[:results:exp238|Parthenolide 2.5μM R05 exp238]]|-1.84|
|[[:results:exp332|Adefovir 20μM R07 exp332]]|-1.75|
|[[:results:exp527|Tanespimycin 14μM R08 exp527]]|1.83|
|[[:results:exp28|Pimelic-diphenylamide-106 5μM R00 exp28]]|2.15|
|[[:results:exp502|Milciclib 2μM R08 exp502]]|2.16|
|[[:results:exp524|Staurosporine 0.02μM R08 exp524]]|2.72|
|[[:results:exp2|5-Fluorouracil 20μM R00 exp2]]|2.95|
|[[:results:exp3|Actinomycin-D 0.001μM R00 exp3]]|3.05|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:u:ube2m|UBE2M]]|0.634|
|[[:human genes:u:uba3|UBA3]]|0.632|
|[[:human genes:t:thg1l|THG1L]]|0.626|
|[[:human genes:g:gfpt1|GFPT1]]|0.619|
|[[:human genes:r:rtcb|RTCB]]|0.608|
|[[:human genes:p:pmm2|PMM2]]|0.604|
|[[:human genes:r:rnmt|RNMT]]|0.591|
|[[:human genes:g:gmppb|GMPPB]]|0.589|
|[[:human genes:t:triap1|TRIAP1]]|0.586|
|[[:human genes:u:uap1|UAP1]]|0.581|
|[[:human genes:r:rpp21|RPP21]]|0.579|
|[[:human genes:a:alg1|ALG1]]|0.576|
|[[:human genes:h:hars|HARS]]|0.576|
|[[:human genes:d:dpagt1|DPAGT1]]|0.574|
|[[:human genes:a:adsl|ADSL]]|0.572|
|[[:human genes:h:hspd1|HSPD1]]|0.555|
|[[:human genes:s:srp14|SRP14]]|0.544|
|[[:human genes:r:rptor|RPTOR]]|0.539|
|[[:human genes:u:uhrf1|UHRF1]]|0.535|
|[[:human genes:t:trmt5|TRMT5]]|0.526|
|[[:human genes:d:dhps|DHPS]]|0.525|
|[[:human genes:a:aars|AARS]]|0.524|
|[[:human genes:m:med16|MED16]]|0.522|
|[[:human genes:a:alg13|ALG13]]|0.521|
|[[:human genes:g:gnb1l|GNB1L]]|0.516|
|[[:human genes:e:exoc4|EXOC4]]|0.516|
|[[:human genes:g:gemin5|GEMIN5]]|0.516|
|[[:human genes:e:eif4e|EIF4E]]|0.512|
|[[:human genes:v:vezt|VEZT]]|0.511|
|[[:human genes:g:gtf3c3|GTF3C3]]|0.509|
|[[:human genes:e:eprs|EPRS]]|0.507|
|[[:human genes:o:osgep|OSGEP]]|0.507|
|[[:human genes:g:gtf3c2|GTF3C2]]|0.505|
|[[:human genes:t:tti2|TTI2]]|0.504|
|[[:human genes:c:cct7|CCT7]]|0.503|
|[[:human genes:p:pelo|PELO]]|0.501|
|[[:human genes:c:cbfb|CBFB]]|0.498|
|[[:human genes:s:sec61g|SEC61G]]|0.498|
|[[:human genes:a:atxn10|ATXN10]]|0.498|
|[[:human genes:a:alg2|ALG2]]|0.498|
|[[:human genes:g:gtf3c4|GTF3C4]]|0.496|
|[[:human genes:d:dr1|DR1]]|0.493|
|[[:human genes:u:ube2l3|UBE2L3]]|0.493|
|[[:human genes:t:trnt1|TRNT1]]|0.493|
|[[:human genes:o:otud5|OTUD5]]|0.493|
|[[:human genes:v:vrk1|VRK1]]|0.491|
|[[:human genes:m:meaf6|MEAF6]]|0.488|
|[[:human genes:b:bap1|BAP1]]|0.486|
|[[:human genes:n:nsmce1|NSMCE1]]|0.485|
|[[:human genes:s:seh1l|SEH1L]]|0.481|
|[[:human genes:u:utp23|UTP23]]|0.48|
|[[:human genes:w:wdr59|WDR59]]|0.48|
|[[:human genes:t:ten1|TEN1]]|0.479|
|[[:human genes:p:pam16|PAM16]]|0.479|
|[[:human genes:d:ddx20|DDX20]]|0.479|
|[[:human genes:a:atp5j|ATP5J]]|0.478|
|[[:human genes:c:cep57|CEP57]]|0.477|
|[[:human genes:g:gemin8|GEMIN8]]|0.477|
|[[:human genes:p:plaa|PLAA]]|0.477|
|[[:human genes:a:alg14|ALG14]]|0.476|
|[[:human genes:g:gtf3c6|GTF3C6]]|0.476|
|[[:human genes:e:eny2|ENY2]]|0.474|
|[[:human genes:g:gemin6|GEMIN6]]|0.471|
|[[:human genes:h:hccs|HCCS]]|0.47|
|[[:human genes:p:ppcs|PPCS]]|0.47|
|[[:human genes:r:rabggtb|RABGGTB]]|0.47|
|[[:human genes:d:dohh|DOHH]]|0.469|
|[[:human genes:h:haus8|HAUS8]]|0.468|
|[[:human genes:c:ccdc101|CCDC101]]|0.468|
|[[:human genes:l:lamtor5|LAMTOR5]]|0.467|
|[[:human genes:p:ppp6c|PPP6C]]|0.465|
|[[:human genes:a:atm|ATM]]|0.463|
|[[:human genes:t:taz|TAZ]]|0.463|
|[[:human genes:e:exoc3|EXOC3]]|0.462|
|[[:human genes:t:tbca|TBCA]]|0.461|
|[[:human genes:y:yars|YARS]]|0.46|
|[[:human genes:t:telo2|TELO2]]|0.459|
|[[:human genes:p:polr3h|POLR3H]]|0.459|
|[[:human genes:r:rad1|RAD1]]|0.458|
|[[:human genes:g:glmn|GLMN]]|0.456|
|[[:human genes:a:apaf1|APAF1]]|0.456|
|[[:human genes:c:cars|CARS]]|0.455|
|[[:human genes:w:wdr24|WDR24]]|0.454|
|[[:human genes:v:vhl|VHL]]|0.454|
|[[:human genes:t:tamm41|TAMM41]]|0.454|
|[[:human genes:f:faf2|FAF2]]|0.454|
|[[:human genes:n:nbn|NBN]]|0.453|
|[[:human genes:t:tsen54|TSEN54]]|0.453|
|[[:human genes:b:bak1|BAK1]]|0.452|
|[[:human genes:c:csnk2b|CSNK2B]]|0.452|
|[[:human genes:c:chchd4|CHCHD4]]|0.451|
|[[:human genes:g:gtf3a|GTF3A]]|0.451|
|[[:human genes:t:txnrd1|TXNRD1]]|0.451|
|[[:human genes:m:med23|MED23]]|0.45|
|[[:human genes:d:ddost|DDOST]]|0.45|
|[[:human genes:t:tprkb|TPRKB]]|0.449|
|[[:human genes:u:ube2k|UBE2K]]|0.447|
|[[:human genes:d:ddx6|DDX6]]|0.447|
|[[:human genes:s:spcs2|SPCS2]]|0.447|
|[[:human genes:n:nup50|NUP50]]|0.443|
|[[:human genes:d:ddx55|DDX55]]|0.443|
|[[:human genes:c:casp9|CASP9]]|0.441|
|[[:human genes:p:pik3r4|PIK3R4]]|0.441|
|[[:human genes:p:ppp4c|PPP4C]]|0.44|
|[[:human genes:t:ttc4|TTC4]]|0.44|
|[[:human genes:a:alg5|ALG5]]|0.44|
|[[:human genes:t:tmem41b|TMEM41B]]|0.439|
|[[:human genes:t:tsen34|TSEN34]]|0.438|
|[[:human genes:s:srp68|SRP68]]|0.438|
|[[:human genes:t:tti1|TTI1]]|0.437|
|[[:human genes:t:tle4|TLE4]]|0.436|
|[[:human genes:o:oraov1|ORAOV1]]|0.436|
|[[:human genes:e:eno1|ENO1]]|0.434|
|[[:human genes:d:dolk|DOLK]]|0.434|
|[[:human genes:t:txnl4b|TXNL4B]]|0.433|
|[[:human genes:m:med25|MED25]]|0.433|
|[[:human genes:d:dad1|DAD1]]|0.433|
|[[:human genes:r:rpe|RPE]]|0.432|
|[[:human genes:t:tgs1|TGS1]]|0.43|
|[[:human genes:u:uxt|UXT]]|0.43|
|[[:human genes:m:mtor|MTOR]]|0.43|
|[[:human genes:t:trim28|TRIM28]]|0.429|
|[[:human genes:p:polr3b|POLR3B]]|0.428|
|[[:human genes:f:fh|FH]]|0.426|
|[[:human genes:s:spcs3|SPCS3]]|0.426|
|[[:human genes:u:ube2g2|UBE2G2]]|0.425|
|[[:human genes:d:dhdds|DHDDS]]|0.425|
|[[:human genes:a:atp5b|ATP5B]]|0.424|
|[[:human genes:u:ubqln1|UBQLN1]]|0.424|
|[[:human genes:n:nars|NARS]]|0.424|
|[[:human genes:p:phb|PHB]]|0.424|
|[[:human genes:p:pggt1b|PGGT1B]]|0.423|
|[[:human genes:m:mllt1|MLLT1]]|0.422|
|[[:human genes:g:gtf2a2|GTF2A2]]|0.421|
|[[:human genes:m:med4|MED4]]|0.42|
|[[:human genes:z:zrsr2|ZRSR2]]|0.42|
|[[:human genes:f:fnta|FNTA]]|0.419|
|[[:human genes:r:rad50|RAD50]]|0.419|
|[[:human genes:t:tsen2|TSEN2]]|0.417|
|[[:human genes:a:alg6|ALG6]]|0.417|
|[[:human genes:p:ppp2r3c|PPP2R3C]]|0.417|
|[[:human genes:a:actr10|ACTR10]]|0.417|
|[[:human genes:a:atic|ATIC]]|0.416|
|[[:human genes:c:cdipt|CDIPT]]|0.415|
|[[:human genes:p:pmpcb|PMPCB]]|0.414|
|[[:human genes:r:romo1|ROMO1]]|0.414|
|[[:human genes:t:tbp|TBP]]|0.413|
|[[:human genes:c:ctc1|CTC1]]|0.411|
|[[:human genes:l:lsm10|LSM10]]|0.41|
|[[:human genes:m:march5|MARCH5]]|0.41|
|[[:human genes:d:dctn6|DCTN6]]|0.41|
|[[:human genes:d:ddx1|DDX1]]|0.409|
|[[:human genes:c:crls1|CRLS1]]|0.409|
|[[:human genes:p:ppa2|PPA2]]|0.408|
|[[:human genes:t:tbcb|TBCB]]|0.408|
|[[:human genes:c:ctdnep1|CTDNEP1]]|0.406|
|[[:human genes:p:pisd|PISD]]|0.406|
|[[:human genes:c:cep350|CEP350]]|0.404|
|[[:human genes:b:btaf1|BTAF1]]|0.403|
|[[:human genes:t:timm13|TIMM13]]|0.403|
|[[:human genes:w:wars|WARS]]|0.402|
|[[:human genes:g:gemin7|GEMIN7]]|0.402|
|[[:human genes:x:xrcc2|XRCC2]]|0.401|
|[[:human genes:s:slc25a26|SLC25A26]]|0.4|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 498/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|18/28|
|blood|13/28|
|bone|25/26|
|breast|22/33|
|central nervous system|33/56|
|cervix|3/4|
|colorectal|14/17|
|esophagus|9/13|
|fibroblast|1/1|
|gastric|6/16|
|kidney|12/21|
|liver|9/20|
|lung|57/75|
|lymphocyte|6/16|
|ovary|17/26|
|pancreas|18/24|
|peripheral nervous system|10/16|
|plasma cell|8/15|
|prostate|1/1|
|skin|17/24|
|soft tissue|5/9|
|thyroid|0/2|
|upper aerodigestive|17/22|
|urinary tract|22/29|
|uterus|5/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 1248
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 6.58 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='NAE1 Expression in NALM6 Cells: 6.58'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>