NAGPA [The Human Chemical-Genetic Interaction Map Project]

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======= NAGPA =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: N/AGPA
  * **<color #00a2e8>Official Name</color>**: N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=51172|51172]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/Q9UK23|Q9UK23]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=NAGPA&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20NAGPA|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/607985|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**:  Hydrolases are transported to lysosomes after binding to mannose 6-phosphate receptors in the trans-Golgi network. This gene encodes the enzyme that catalyzes the second step in the formation of the mannose 6-phosphate recognition marker on lysosomal hydrolases. Commonly known as 'uncovering enzyme' or UCE, this enzyme removes N-acetyl-D-glucosamine (GlcNAc) residues from GlcNAc-alpha-P-mannose moieties and thereby produces the recognition marker. The encoded preproprotein is proteolytically processed by furin to generate the mature enzyme, a homotetramer of two disulfide-linked homodimers. Mutations in this gene are associated with developmental stuttering in human patients. [provided by RefSeq, Oct 2015]. 
  * **<color #00a2e8>UniProt Summary</color>**:  Catalyzes the second step in the formation of the mannose 6-phosphate targeting signal on lysosomal enzyme oligosaccharides by removing GlcNAc residues from GlcNAc-alpha-P- mannose moieties, which are formed in the first step. Also hydrolyzes UDP-GlcNAc, a sugar donor for Golgi N- acetylglucosaminyltransferases. {ECO:0000269|PubMed:23572527}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|DUF2233|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase activity|
|secretion of lysosomal enzymes|
|protein targeting to lysosome|
|protein targeting to vacuole|
|protein localization to lysosome|
|establishment of protein localization to vacuole|
|lytic vacuole organization|
|lysosome organization|
|protein localization to vacuole|
|Golgi cisterna membrane|
|lysosomal transport|
|vacuole organization|
|vacuolar transport|
|protein secretion|
|establishment of protein localization to extracellular region|
|protein localization to extracellular region|
|peptide secretion|
|protein glycosylation|
|macromolecule glycosylation|
|glycosylation|
|glycoprotein biosynthetic process|
|protein targeting|
|glycoprotein metabolic process|
|establishment of protein localization to organelle|
|carbohydrate metabolic process|
|carbohydrate derivative biosynthetic process|
|protein localization to organelle|
|intracellular protein transport|
|secretion by cell|
|carbohydrate derivative metabolic process|
|export from cell|
|secretion|
|organonitrogen compound biosynthetic process|
|protein transport|
|intracellular transport|
|peptide transport|
|amide transport|
|cellular protein localization|
|cellular macromolecule localization|
|establishment of protein localization|
|cellular macromolecule biosynthetic process|
|macromolecule biosynthetic process|
|establishment of localization in cell|
|nitrogen compound transport|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='default' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp490|Hydroxychloroquine 30μM R08 exp490]]|-1.84|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
No correlation found to any other genes in chemogenomics.
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 0/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|0/28|
|blood|0/28|
|bone|0/26|
|breast|0/33|
|central nervous system|0/56|
|cervix|0/4|
|colorectal|0/17|
|esophagus|0/13|
|fibroblast|0/1|
|gastric|0/16|
|kidney|0/21|
|liver|0/20|
|lung|0/75|
|lymphocyte|0/16|
|ovary|0/26|
|pancreas|0/24|
|peripheral nervous system|0/16|
|plasma cell|0/15|
|prostate|0/1|
|skin|0/24|
|soft tissue|0/9|
|thyroid|0/2|
|upper aerodigestive|0/22|
|urinary tract|0/29|
|uterus|0/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 7412
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 4.81 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='NAGPA Expression in NALM6 Cells: 4.81'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>