NANS [The Human Chemical-Genetic Interaction Map Project]

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======= NANS =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: N/ANS
  * **<color #00a2e8>Official Name</color>**: N-acetylneuraminate synthase
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=54187|54187]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/Q9NR45|Q9NR45]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=NANS&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20NANS|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/605202|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**:  This gene encodes an enzyme that functions in the biosynthetic pathways of sialic acids. In vitro, the encoded protein uses N-acetylmannosamine 6-phosphate and mannose 6-phosphate as substrates to generate phosphorylated forms of N-acetylneuraminic acid (Neu5Ac) and 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid (KDN), respectively; however, it exhibits much higher activity toward the Neu5Ac phosphate product. In insect cells, expression of this gene results in Neu5Ac and KDN production. This gene is related to the E. coli sialic acid synthase gene neuB, and it can partially restore sialic acid synthase activity in an E. coli neuB-negative mutant. [provided by RefSeq, Jul 2008]. 
  * **<color #00a2e8>UniProt Summary</color>**:  Produces N-acetylneuraminic acid (Neu5Ac) and 2-keto-3- deoxy-D-glycero-D-galacto-nononic acid (KDN). Can also use N- acetylmannosamine 6-phosphate and mannose 6-phosphate as substrates to generate phosphorylated forms of Neu5Ac and KDN, respectively.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|SAF|
|NeuB|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|N-acylneuraminate-9-phosphate synthase activity|
|CMP-N-acetylneuraminate biosynthetic process|
|N-acetylneuraminate synthase activity|
|CMP-N-acetylneuraminate metabolic process|
|N-acylneuraminate cytidylyltransferase activity|
|nucleotide-sugar biosynthetic process|
|nucleotide-sugar metabolic process|
|carbohydrate biosynthetic process|
|carbohydrate metabolic process|
|nucleobase-containing small molecule metabolic process|
|carbohydrate derivative biosynthetic process|
|carbohydrate derivative metabolic process|
|nucleobase-containing compound biosynthetic process|
|heterocycle biosynthetic process|
|aromatic compound biosynthetic process|
|organic cyclic compound biosynthetic process|
|cellular nitrogen compound biosynthetic process|
|small molecule metabolic process|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp420|Tunicamycin 0.04 to 0.125μM  on day4 R07 exp420]]|-2.89|
|[[:results:exp419|Tunicamycin 0.04 to 0.075μM  on day4 R07 exp419]]|-2.29|
|[[:results:exp148|SB202190 10μM R03 exp148]]|-1.97|
|[[:results:exp520|Rucaparib 6.5μM R08 exp520]]|-1.93|
|[[:results:exp147|Resveratrol 16μM R03 exp147]]|-1.86|
|[[:results:exp111|R-DABN 8μM R03 exp111]]|-1.8|
|[[:results:exp190|Vincristine 0.0005μM R04 exp190]]|-1.74|
|[[:results:exp450|Artemisinin 50μM R08 exp450]]|1.75|
|[[:results:exp483|FTY720 3μM R08 exp483]]|1.76|
|[[:results:exp372|Ibrutinib 1μM R07 exp372]]|1.76|
|[[:results:exp233|EPZ-5676 30μM R05 exp233]]|1.78|
|[[:results:exp399|Salubrinal 20μM R07 exp399]]|1.78|
|[[:results:exp359|FK-506 30μM R07 exp359]]|1.81|
|[[:results:exp29|Rapamycin 1μM R00 exp29]]|1.82|
|[[:results:exp72|LB-100 4.1μM R02 exp72]]|1.86|
|[[:results:exp485|GSK626616 14μM R08 exp485]]|1.9|
|[[:results:exp429|Rapamycin 0.001μM R08 exp429]]|1.92|
|[[:results:exp498|Lead acetate 2000μM R08 exp498 no dilution day6]]|1.95|
|[[:results:exp470|Chloroquine 32μM R08 exp470]]|1.97|
|[[:results:exp478|Doxorubicin 0.02μM R08 exp478]]|1.99|
|[[:results:exp497|Lead acetate 2000μM R08 exp497]]|2|
|[[:results:exp348|Cyclosporin-A 3μM R07 exp348]]|2.02|
|[[:results:exp417|Tubastatin-A 2.5μM R07 exp417]]|2.02|
|[[:results:exp264|Arsenate 40μM R06 exp264]]|2.03|
|[[:results:exp360|Genistein 15μM R07 exp360]]|2.08|
|[[:results:exp218|A-395 10μM R05 exp218]]|2.12|
|[[:results:exp346|CoCl2 18μM R07 exp346]]|2.12|
|[[:results:exp294|Nutlin-3A 1.6μM R06 exp294]]|2.12|
|[[:results:exp85|UM0129480 7μM R02 exp85]]|2.13|
|[[:results:exp308|Rapamycin 2μM plus FK-506 5μM R07 exp308]]|2.18|
|[[:results:exp449|Arsenic trioxide 60μM R08 exp449]]|2.25|
|[[:results:exp351|Dexamethasone 0.006μM R07 exp351]]|2.53|
|[[:results:exp240|Pyridostatin 4μM R05 exp240]]|2.66|
|[[:results:exp489|Hippuristanol 0.12μM R08 exp489 no dilution day6]]|2.67|
|[[:results:exp306|Rapamycin 2μM R07 exp306]]|2.73|
|[[:results:exp30|Rapamycin 10μM R00 exp30]]|2.77|
|[[:results:exp342|Calcium Ionophore 0.4μM R07 exp342]]|3.04|
|[[:results:exp492|iCRT14 30μM R08 exp492]]|3.04|
|[[:results:exp488|Hippuristanol 0.12μM R08 exp488]]|3.21|
|[[:results:exp307|Rapamycin 2μM plus Cyclosporin-A 3μM R07 exp307]]|3.23|
|[[:results:exp25|Oligomycin-A 2μM R00 exp25]]|3.32|
|[[:results:exp189|Temozolomide 200μM R04 exp189]]|3.52|
|[[:results:exp216|Erlotinib 10μM R05 exp216]]|3.64|
|[[:results:exp407|Thapsigargin 0.005μM R07 exp407]]|3.76|
|[[:results:exp352|Dexamethasone 0.006 to 0.015μM on day4 R07 exp352]]|4.11|
|[[:results:exp504|MK2206 4μM R08 exp504]]|4.49|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:c:cmas|CMAS]]|0.486|
|[[:human genes:a:abhd11|ABHD11]]|0.415|
|[[:human genes:z:zfr|ZFR]]|0.413|
|[[:human genes:t:tsc2|TSC2]]|0.41|
|[[:human genes:g:gsk3b|GSK3B]]|0.407|
|[[:human genes:n:nprl2|NPRL2]]|0.402|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 0/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|0/28|
|blood|0/28|
|bone|0/26|
|breast|0/33|
|central nervous system|0/56|
|cervix|0/4|
|colorectal|0/17|
|esophagus|0/13|
|fibroblast|0/1|
|gastric|0/16|
|kidney|0/21|
|liver|0/20|
|lung|0/75|
|lymphocyte|0/16|
|ovary|0/26|
|pancreas|0/24|
|peripheral nervous system|0/16|
|plasma cell|0/15|
|prostate|0/1|
|skin|0/24|
|soft tissue|0/9|
|thyroid|0/2|
|upper aerodigestive|0/22|
|urinary tract|0/29|
|uterus|0/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 5825
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 5.68 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='NANS Expression in NALM6 Cells: 5.68'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>