NCF4 [The Human Chemical-Genetic Interaction Map Project]

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======= NCF4 =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: NCF4
  * **<color #00a2e8>Official Name</color>**: neutrophil cytosolic factor 4
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=4689|4689]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/Q15080|Q15080]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=NCF4&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20NCF4|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/601488|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**:  The protein encoded by this gene is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]. 
  * **<color #00a2e8>UniProt Summary</color>**:  Component of the NADPH-oxidase, a multicomponent enzyme system responsible for the oxidative burst in which electrons are transported from NADPH to molecular oxygen, generating reactive oxidant intermediates. It may be important for the assembly and/or activation of the NADPH-oxidase complex. {ECO:0000269|PubMed:8280052}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|SH3 2|
|PX|
|SH3 1|
|PB1|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|phagolysosome|
|superoxide-generating NADPH oxidase activator activity|
|NADPH oxidase complex|
|respiratory burst|
|superoxide metabolic process|
|phosphatidylinositol-3-phosphate binding|
|vascular endothelial growth factor receptor signaling pathway|
|antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent|
|cell redox homeostasis|
|antigen processing and presentation of exogenous peptide antigen via MHC class I|
|phosphatidylinositol binding|
|antigen processing and presentation of peptide antigen via MHC class I|
|reactive oxygen species metabolic process|
|protein dimerization activity|
|antigen processing and presentation of exogenous peptide antigen|
|antigen processing and presentation of exogenous antigen|
|antigen processing and presentation of peptide antigen|
|endosome membrane|
|antigen processing and presentation|
|cellular response to oxidative stress|
|phagocytosis|
|response to oxidative stress|
|transmembrane receptor protein tyrosine kinase signaling pathway|
|enzyme linked receptor protein signaling pathway|
|cellular homeostasis|
|oxidation-reduction process|
|positive regulation of catalytic activity|
|homeostatic process|
|cellular response to stress|
|positive regulation of molecular function|
|immune response|
|vesicle-mediated transport|
|membrane|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='default' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp301|VER-155008 3.9μM R06 exp301]]|-1.94|
|[[:results:exp60|Vinblastine 0.002μM R01 exp60]]|1.82|
|[[:results:exp456|Benzoate 20000μM R08 exp456]]|1.91|
|[[:results:exp100|NFN1 1μM R03 exp100]]|2.07|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
No correlation found to any other genes in chemogenomics.
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 0/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|0/28|
|blood|0/28|
|bone|0/26|
|breast|0/33|
|central nervous system|0/56|
|cervix|0/4|
|colorectal|0/17|
|esophagus|0/13|
|fibroblast|0/1|
|gastric|0/16|
|kidney|0/21|
|liver|0/20|
|lung|0/75|
|lymphocyte|0/16|
|ovary|0/26|
|pancreas|0/24|
|peripheral nervous system|0/16|
|plasma cell|0/15|
|prostate|0/1|
|skin|0/24|
|soft tissue|0/9|
|thyroid|0/2|
|upper aerodigestive|0/22|
|urinary tract|0/29|
|uterus|0/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 14724
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 6.4 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='NCF4 Expression in NALM6 Cells: 6.4'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>