PICALM [The Human Chemical-Genetic Interaction Map Project]

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======= PICALM =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: PICALM
  * **<color #00a2e8>Official Name</color>**: phosphatidylinositol binding clathrin assembly protein
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=8301|8301]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/Q13492|Q13492]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=PICALM&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20PICALM|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/603025|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**:  This gene encodes a clathrin assembly protein, which recruits clathrin and adaptor protein complex 2 (AP2) to cell membranes at sites of coated-pit formation and clathrin-vesicle assembly. The protein may be required to determine the amount of membrane to be recycled, possibly by regulating the size of the clathrin cage. The protein is involved in AP2-dependent clathrin-mediated endocytosis at the neuromuscular junction. A chromosomal translocation t(10;11)(p13;q14) leading to the fusion of this gene and the MLLT10 gene is found in acute lymphoblastic leukemia, acute myeloid leukemia and malignant lymphomas. The polymorphisms of this gene are associated with the risk of Alzheimer disease. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2011]. 
  * **<color #00a2e8>UniProt Summary</color>**:  Assembly protein recruiting clathrin and adapter protein complex 2 (AP2) to cell membranes at sites of coated-pit formation and clathrin-vesicle assembly. May be required to determine the amount of membrane to be recycled, possibly by regulating the size of the clathrin cage. Involved in AP2-dependent clathrin-mediated endocytosis at the neuromuscular junction. {ECO:0000269|PubMed:10436022}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|ENTH|
|ANTH|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|endosome to plasma membrane transport vesicle|
|negative regulation of metalloendopeptidase activity involved in amyloid precursor protein catabolic process|
|clathrin-coated pit assembly|
|membrane bending|
|regulation of metalloendopeptidase activity involved in amyloid precursor protein catabolic process|
|extrinsic component of presynaptic endocytic zone membrane|
|negative regulation of metalloendopeptidase activity|
|regulation of vesicle size|
|neurofibrillary tangle|
|synaptic vesicle budding from presynaptic endocytic zone membrane|
|negative regulation of metallopeptidase activity|
|clathrin coat of coated pit|
|regulation of metalloendopeptidase activity|
|positive regulation of aspartic-type endopeptidase activity involved in amyloid precursor protein catabolic process|
|amyloid-beta clearance by transcytosis|
|positive regulation of aspartic-type peptidase activity|
|clathrin heavy chain binding|
|synaptic vesicle budding|
|regulation of aspartic-type endopeptidase activity involved in amyloid precursor protein catabolic process|
|modulation of age-related behavioral decline|
|regulation of aspartic-type peptidase activity|
|synaptic vesicle maturation|
|intrinsic component of membrane|
|negative regulation of protein localization to cell surface|
|regulation of metallopeptidase activity|
|AP-2 adaptor complex|
|negative regulation of amyloid precursor protein catabolic process|
|positive regulation of amyloid-beta formation|
|1-phosphatidylinositol binding|
|transcytosis|
|clathrin coat assembly|
|positive regulation of amyloid precursor protein catabolic process|
|low-density lipoprotein particle receptor binding|
|negative regulation of protein localization to plasma membrane|
|amyloid-beta clearance|
|negative regulation of receptor-mediated endocytosis|
|negative regulation of protein localization to cell periphery|
|clathrin-dependent endocytosis|
|clathrin-coated endocytic vesicle|
|vesicle cargo loading|
|regulation of amyloid-beta formation|
|negative regulation of protein localization to membrane|
|regulation of amyloid precursor protein catabolic process|
|synaptic vesicle transport|
|regulation of protein localization to cell surface|
|clathrin binding|
|synaptic vesicle localization|
|presynaptic endocytosis|
|synaptic vesicle endocytosis|
|negative regulation of endocytosis|
|tau protein binding|
|synaptic vesicle recycling|
|clathrin-coated pit|
|clathrin-coated vesicle|
|dendrite morphogenesis|
|receptor internalization|
|SNARE binding|
|presynaptic membrane|
|phosphatidylinositol-4,5-bisphosphate binding|
|iron ion homeostasis|
|amyloid-beta binding|
|positive regulation of neuron death|
|regulation of protein localization to plasma membrane|
|vesicle budding from membrane|
|regulation of receptor-mediated endocytosis|
|dendrite development|
|receptor metabolic process|
|negative regulation of cellular protein localization|
|regulation of protein localization to cell periphery|
|synaptic vesicle cycle|
|synaptic vesicle|
|vesicle-mediated transport in synapse|
|transition metal ion homeostasis|
|vesicle|
|Rab GTPase binding|
|positive regulation of cellular amide metabolic process|
|positive regulation of endopeptidase activity|
|establishment of vesicle localization|
|vesicle localization|
|regulation of protein localization to membrane|
|positive regulation of peptidase activity|
|postsynaptic membrane|
|regulation of endocytosis|
|endosomal transport|
|receptor-mediated endocytosis|
|developmental maturation|
|negative regulation of endopeptidase activity|
|negative regulation of peptidase activity|
|early endosome|
|learning or memory|
|aging|
|cognition|
|vesicle organization|
|cadherin binding|
|regulation of neuron death|
|negative regulation of proteolysis|
|positive regulation of proteolysis|
|establishment of organelle localization|
|neuronal cell body|
|axonogenesis|
|regulation of cellular component size|
|regulation of cellular amide metabolic process|
|axon development|
|positive regulation of GTPase activity|
|regulation of endopeptidase activity|
|cell morphogenesis involved in neuron differentiation|
|regulation of peptidase activity|
|negative regulation of hydrolase activity|
|neuron projection morphogenesis|
|regulation of GTPase activity|
|plasma membrane bounded cell projection morphogenesis|
|negative regulation of transport|
|cell projection morphogenesis|
|cell part morphogenesis|
|regulation of anatomical structure size|
|regulation of cellular protein localization|
|regulation of vesicle-mediated transport|
|endocytosis|
|cell morphogenesis involved in differentiation|
|hemopoiesis|
|behavior|
|organelle localization|
|hematopoietic or lymphoid organ development|
|cell surface|
|metal ion homeostasis|
|immune system development|
|neuron projection development|
|import into cell|
|intracellular membrane-bounded organelle|
|perinuclear region of cytoplasm|
|positive regulation of cell death|
|negative regulation of cellular component organization|
|cation homeostasis|
|inorganic ion homeostasis|
|cell morphogenesis|
|regulation of proteolysis|
|positive regulation of hydrolase activity|
|ion homeostasis|
|negative regulation of catalytic activity|
|neuron development|
|cellular component morphogenesis|
|cellular protein-containing complex assembly|
|membrane organization|
|regulation of cellular localization|
|Golgi apparatus|
|neuron differentiation|
|regulation of protein localization|
|negative regulation of cellular protein metabolic process|
|negative regulation of protein metabolic process|
|chemical homeostasis|
|plasma membrane bounded cell projection organization|
|negative regulation of molecular function|
|cell projection organization|
|regulation of hydrolase activity|
|nervous system process|
|positive regulation of catalytic activity|
|intracellular transport|
|generation of neurons|
|positive regulation of transcription, DNA-templated|
|protein-containing complex assembly|
|positive regulation of cellular protein metabolic process|
|neurogenesis|
|positive regulation of nucleic acid-templated transcription|
|positive regulation of RNA biosynthetic process|
|homeostatic process|
|cell development|
|regulation of cell death|
|positive regulation of protein metabolic process|
|negative regulation of gene expression|
|positive regulation of RNA metabolic process|
|positive regulation of molecular function|
|establishment of localization in cell|
|protein-containing complex subunit organization|
|regulation of transport|
|positive regulation of nucleobase-containing compound metabolic process|
|positive regulation of macromolecule biosynthetic process|
|vesicle-mediated transport|
|system process|
|positive regulation of cellular biosynthetic process|
|positive regulation of gene expression|
|membrane|
|positive regulation of biosynthetic process|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='default' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp434|Vemurafenib 6.6μM R08 exp434]]|-2.71|
|[[:results:exp210|LB-100 2μM R05 exp210]]|-1.87|
|[[:results:exp72|LB-100 4.1μM R02 exp72]]|-1.76|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
No correlation found to any other genes in chemogenomics.
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 1/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|0/28|
|blood|1/28|
|bone|0/26|
|breast|0/33|
|central nervous system|0/56|
|cervix|0/4|
|colorectal|0/17|
|esophagus|0/13|
|fibroblast|0/1|
|gastric|0/16|
|kidney|0/21|
|liver|0/20|
|lung|0/75|
|lymphocyte|0/16|
|ovary|0/26|
|pancreas|0/24|
|peripheral nervous system|0/16|
|plasma cell|0/15|
|prostate|0/1|
|skin|0/24|
|soft tissue|0/9|
|thyroid|0/2|
|upper aerodigestive|0/22|
|urinary tract|0/29|
|uterus|0/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 18996
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 6.45 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='PICALM Expression in NALM6 Cells: 6.45'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>