PRKCG [The Human Chemical-Genetic Interaction Map Project]

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======= PRKCG =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: PRKCG
  * **<color #00a2e8>Official Name</color>**: protein kinase C gamma
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=5582|5582]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/P05129|P05129]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=PRKCG&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20PRKCG|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/176980|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**:  Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. The protein encoded by this gene is one of the PKC family members. This protein kinase is expressed solely in the brain and spinal cord and its localization is restricted to neurons. It has been demonstrated that several neuronal functions, including long term potentiation (LTP) and long term depression (LTD), specifically require this kinase. Knockout studies in mice also suggest that this kinase may be involved in neuropathic pain development. Defects in this protein have been associated with neurodegenerative disorder spinocerebellar ataxia-14 (SCA14). Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2015]. 
  * **<color #00a2e8>UniProt Summary</color>**:  Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that plays diverse roles in neuronal cells and eye tissues, such as regulation of the neuronal receptors GRIA4/GLUR4 and GRIN1/NMDAR1, modulation of receptors and neuronal functions related to sensitivity to opiates, pain and alcohol, mediation of synaptic function and cell survival after ischemia, and inhibition of gap junction activity after oxidative stress. Binds and phosphorylates GRIA4/GLUR4 glutamate receptor and regulates its function by increasing plasma membrane-associated GRIA4 expression. In primary cerebellar neurons treated with the agonist 3,5-dihyidroxyphenylglycine, functions downstream of the metabotropic glutamate receptor GRM5/MGLUR5 and phosphorylates GRIN1/NMDAR1 receptor which plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. May be involved in the regulation of hippocampal long-term potentiation (LTP), but may be not necessary for the process of synaptic plasticity. May be involved in desensitization of mu-type opioid receptor-mediated G-protein activation in the spinal cord, and may be critical for the development and/or maintenance of morphine-induced reinforcing effects in the limbic forebrain. May modulate the functionality of mu-type-opioid receptors by participating in a signaling pathway which leads to the phosphorylation and degradation of opioid receptors. May also contributes to chronic morphine-induced changes in nociceptive processing. Plays a role in neuropathic pain mechanisms and contributes to the maintenance of the allodynia pain produced by peripheral inflammation. Plays an important role in initial sensitivity and tolerance to ethanol, by mediating the behavioral effects of ethanol as well as the effects of this drug on the GABA(A) receptors. During and after cerebral ischemia modulate neurotransmission and cell survival in synaptic membranes, and is involved in insulin-induced inhibition of necrosis, an important mechanism for minimizing ischemic injury. Required for the elimination of multiple climbing fibers during innervation of Purkinje cells in developing cerebellum. Is activated in lens epithelial cells upon hydrogen peroxide treatment, and phosphorylates connexin-43 (GJA1/CX43), resulting in disassembly of GJA1 gap junction plaques and inhibition of gap junction activity which could provide a protective effect against oxidative stress (By similarity). Phosphorylates p53/TP53 and promotes p53/TP53-dependent apoptosis in response to DNA damage. Involved in the phase resetting of the cerebral cortex circadian clock during temporally restricted feeding. Stabilizes the core clock component ARNTL/BMAL1 by interfering with its ubiquitination, thus suppressing its degradation, resulting in phase resetting of the cerebral cortex clock (By similarity). {ECO:0000250|UniProtKB:P63318, ECO:0000250|UniProtKB:P63319, ECO:0000269|PubMed:16377624}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|C2|
|Pkinase|
|Pkinase Tyr|
|Pkinase C|
|C1 1|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|calcium-dependent protein kinase C activity|
|positive regulation of mismatch repair|
|regulation of mismatch repair|
|response to psychosocial stress|
|presynaptic cytosol|
|presynaptic modulation of chemical synaptic transmission|
|protein kinase C activity|
|postsynaptic cytosol|
|chemosensory behavior|
|regulation of response to food|
|calyx of Held|
|regulation of response to nutrient levels|
|regulation of response to extracellular stimulus|
|synaptic membrane|
|innervation|
|protein serine/threonine/tyrosine kinase activity|
|response to pain|
|response to isoquinoline alkaloid|
|response to morphine|
|negative regulation of proteasomal protein catabolic process|
|positive regulation of DNA repair|
|multicellular organismal response to stress|
|negative regulation of proteolysis involved in cellular protein catabolic process|
|negative regulation of protein ubiquitination|
|response to anesthetic|
|regulation of synaptic vesicle exocytosis|
|nerve development|
|negative regulation of protein modification by small protein conjugation or removal|
|negative regulation of cellular protein catabolic process|
|regulation of phagocytosis|
|positive regulation of response to DNA damage stimulus|
|regulation of neurotransmitter secretion|
|response to alkaloid|
|regulation of synaptic vesicle cycle|
|regulation of circadian rhythm|
|regulation of DNA repair|
|negative regulation of protein catabolic process|
|platelet activation|
|regulation of neurotransmitter transport|
|negative regulation of neuron apoptotic process|
|regulation of regulated secretory pathway|
|cell-cell junction|
|peptidyl-serine phosphorylation|
|regulation of proteasomal protein catabolic process|
|response to ammonium ion|
|positive regulation of DNA metabolic process|
|protein autophosphorylation|
|peptidyl-serine modification|
|regulation of protein ubiquitination|
|negative regulation of neuron death|
|regulation of neuron apoptotic process|
|regulation of proteolysis involved in cellular protein catabolic process|
|regulation of response to DNA damage stimulus|
|regulation of exocytosis|
|regulation of protein modification by small protein conjugation or removal|
|protein kinase activity|
|regulation of cellular protein catabolic process|
|negative regulation of cellular catabolic process|
|postsynaptic density|
|learning or memory|
|rhythmic process|
|blood coagulation|
|coagulation|
|hemostasis|
|cognition|
|response to xenobiotic stimulus|
|negative regulation of catabolic process|
|regulation of neuron death|
|regulation of neurotransmitter levels|
|negative regulation of proteolysis|
|regulation of DNA metabolic process|
|protein serine/threonine kinase activity|
|regulation of protein catabolic process|
|anterograde trans-synaptic signaling|
|dendrite|
|chemical synaptic transmission|
|trans-synaptic signaling|
|modulation of chemical synaptic transmission|
|regulation of trans-synaptic signaling|
|synaptic signaling|
|wound healing|
|regulation of body fluid levels|
|regulation of vesicle-mediated transport|
|behavior|
|response to wounding|
|negative regulation of protein modification process|
|perinuclear region of cytoplasm|
|regulation of proteolysis|
|regulation of cellular response to stress|
|regulation of secretion by cell|
|regulation of secretion|
|regulation of cellular catabolic process|
|zinc ion binding|
|peptidyl-amino acid modification|
|negative regulation of apoptotic process|
|negative regulation of programmed cell death|
|regulation of cellular localization|
|response to organic cyclic compound|
|protein phosphorylation|
|negative regulation of cell death|
|regulation of catabolic process|
|response to organonitrogen compound|
|response to drug|
|negative regulation of cellular protein metabolic process|
|cell activation|
|response to nitrogen compound|
|regulation of response to external stimulus|
|negative regulation of protein metabolic process|
|cell-cell signaling|
|phosphorylation|
|nervous system process|
|regulation of response to stress|
|ATP binding|
|regulation of apoptotic process|
|regulation of programmed cell death|
|regulation of cell death|
|intracellular signal transduction|
|regulation of protein modification process|
|regulation of transport|
|positive regulation of nucleobase-containing compound metabolic process|
|system process|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='default' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp340|BN82002 4μM R07 exp340]]|-1.83|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
No correlation found to any other genes in chemogenomics.
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 0/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|0/28|
|blood|0/28|
|bone|0/26|
|breast|0/33|
|central nervous system|0/56|
|cervix|0/4|
|colorectal|0/17|
|esophagus|0/13|
|fibroblast|0/1|
|gastric|0/16|
|kidney|0/21|
|liver|0/20|
|lung|0/75|
|lymphocyte|0/16|
|ovary|0/26|
|pancreas|0/24|
|peripheral nervous system|0/16|
|plasma cell|0/15|
|prostate|0/1|
|skin|0/24|
|soft tissue|0/9|
|thyroid|0/2|
|upper aerodigestive|0/22|
|urinary tract|0/29|
|uterus|0/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 11633
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: -0.98 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='PRKCG Expression in NALM6 Cells: -0.98'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>