PRKCH [The Human Chemical-Genetic Interaction Map Project]

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======= PRKCH =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: PRKCH
  * **<color #00a2e8>Official Name</color>**: protein kinase C eta
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=5583|5583]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/P24723|P24723]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=PRKCH&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20PRKCH|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/605437|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**:  Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipids-dependent protein kinase. It is predominantly expressed in epithelial tissues and has been shown to reside specifically in the cell nucleus. This protein kinase can regulate keratinocyte differentiation by activating the MAP kinase MAPK13 (p38delta)-activated protein kinase cascade that targets CCAAT/enhancer-binding protein alpha (CEBPA). It is also found to mediate the transcription activation of the transglutaminase 1 (TGM1) gene. Mutations in this gene are associated with susceptibility to cerebral infarction. [provided by RefSeq, Sep 2015]. 
  * **<color #00a2e8>UniProt Summary</color>**:  Calcium-independent, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that is involved in the regulation of cell differentiation in keratinocytes and pre-B cell receptor, mediates regulation of epithelial tight junction integrity and foam cell formation, and is required for glioblastoma proliferation and apoptosis prevention in MCF-7 cells. In keratinocytes, binds and activates the tyrosine kinase FYN, which in turn blocks epidermal growth factor receptor (EGFR) signaling and leads to keratinocyte growth arrest and differentiation. Associates with the cyclin CCNE1-CDK2-CDKN1B complex and inhibits CDK2 kinase activity, leading to RB1 dephosphorylation and thereby G1 arrest in keratinocytes. In association with RALA activates actin depolymerization, which is necessary for keratinocyte differentiation. In the pre-B cell receptor signaling, functions downstream of BLNK by up-regulating IRF4, which in turn activates L chain gene rearrangement. Regulates epithelial tight junctions (TJs) by phosphorylating occludin (OCLN) on threonine residues, which is necessary for the assembly and maintenance of TJs. In association with PLD2 and via TLR4 signaling, is involved in lipopolysaccharide (LPS)-induced RGS2 down-regulation and foam cell formation. Upon PMA stimulation, mediates glioblastoma cell proliferation by activating the mTOR pathway, the PI3K/AKT pathway and the ERK1- dependent phosphorylation of ELK1. Involved in the protection of glioblastoma cells from irradiation-induced apoptosis by preventing caspase-9 activation. In camptothecin-treated MCF-7 cells, regulates NF-kappa-B upstream signaling by activating IKBKB, and confers protection against DNA damage-induced apoptosis. Promotes oncogenic functions of ATF2 in the nucleus while blocking its apoptotic function at mitochondria. Phosphorylates ATF2 which promotes its nuclear retention and transcriptional activity and negatively regulates its mitochondrial localization. {ECO:0000269|PubMed:10806212, ECO:0000269|PubMed:11112424, ECO:0000269|PubMed:11772428, ECO:0000269|PubMed:15489897, ECO:0000269|PubMed:17146445, ECO:0000269|PubMed:18780722, ECO:0000269|PubMed:19114660, ECO:0000269|PubMed:20558593, ECO:0000269|PubMed:21820409, ECO:0000269|PubMed:22304920}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|Pkinase|
|C2|
|Pkinase C|
|Pkinase Tyr|
|C1 1|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|negative regulation of glial cell apoptotic process|
|positive regulation of B cell receptor signaling pathway|
|regulation of glial cell apoptotic process|
|protein kinase C activity|
|positive regulation of glial cell proliferation|
|positive regulation of keratinocyte differentiation|
|positive regulation of macrophage derived foam cell differentiation|
|regulation of bicellular tight junction assembly|
|positive regulation of antigen receptor-mediated signaling pathway|
|positive regulation of epidermal cell differentiation|
|regulation of B cell receptor signaling pathway|
|regulation of macrophage derived foam cell differentiation|
|regulation of glial cell proliferation|
|positive regulation of epidermis development|
|regulation of keratinocyte differentiation|
|positive regulation of protein localization to plasma membrane|
|positive regulation of epithelial cell differentiation|
|regulation of epidermal cell differentiation|
|positive regulation of protein localization to cell periphery|
|regulation of antigen receptor-mediated signaling pathway|
|positive regulation of gliogenesis|
|regulation of epidermis development|
|regulation of cell junction assembly|
|regulation of protein localization to plasma membrane|
|regulation of protein localization to cell periphery|
|regulation of gliogenesis|
|positive regulation of protein localization to membrane|
|platelet activation|
|regulation of epithelial cell differentiation|
|positive regulation of NF-kappaB transcription factor activity|
|peptidyl-serine phosphorylation|
|regulation of protein localization to membrane|
|peptidyl-serine modification|
|positive regulation of DNA-binding transcription factor activity|
|blood coagulation|
|coagulation|
|hemostasis|
|positive regulation of cellular protein localization|
|enzyme binding|
|protein serine/threonine kinase activity|
|regulation of DNA-binding transcription factor activity|
|positive regulation of neurogenesis|
|wound healing|
|regulation of body fluid levels|
|positive regulation of nervous system development|
|regulation of cellular protein localization|
|positive regulation of cell development|
|response to wounding|
|regulation of neurogenesis|
|positive regulation of immune response|
|peptidyl-amino acid modification|
|negative regulation of apoptotic process|
|negative regulation of programmed cell death|
|positive regulation of cell population proliferation|
|regulation of cellular localization|
|regulation of nervous system development|
|regulation of cell development|
|regulation of cellular component biogenesis|
|positive regulation of cell differentiation|
|protein phosphorylation|
|negative regulation of cell death|
|regulation of protein localization|
|cell activation|
|regulation of immune response|
|positive regulation of immune system process|
|phosphorylation|
|positive regulation of developmental process|
|ATP binding|
|generation of neurons|
|regulation of apoptotic process|
|regulation of programmed cell death|
|regulation of cell population proliferation|
|neurogenesis|
|regulation of immune system process|
|positive regulation of signal transduction|
|regulation of cell death|
|intracellular signal transduction|
|positive regulation of multicellular organismal process|
|positive regulation of molecular function|
|regulation of cell differentiation|
|positive regulation of cell communication|
|positive regulation of signaling|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp96|BI-2536 0.02μM R03 exp96]]|-1.72|
|[[:results:exp115|A-366 10μM R03 exp115]]|1.72|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:r:rrm1|RRM1]]|0.549|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 0/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|0/28|
|blood|0/28|
|bone|0/26|
|breast|0/33|
|central nervous system|0/56|
|cervix|0/4|
|colorectal|0/17|
|esophagus|0/13|
|fibroblast|0/1|
|gastric|0/16|
|kidney|0/21|
|liver|0/20|
|lung|0/75|
|lymphocyte|0/16|
|ovary|0/26|
|pancreas|0/24|
|peripheral nervous system|0/16|
|plasma cell|0/15|
|prostate|0/1|
|skin|0/24|
|soft tissue|0/9|
|thyroid|0/2|
|upper aerodigestive|0/22|
|urinary tract|0/29|
|uterus|0/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 11835
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 2.68 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='PRKCH Expression in NALM6 Cells: 2.68'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>