RAB7A [The Human Chemical-Genetic Interaction Map Project]

This page is read only. You can view the source, but not change it. Ask your administrator if you think this is wrong.
======= RAB7A =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: RAB7A
  * **<color #00a2e8>Official Name</color>**: RAB7A, member RAS oncogene family
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=7879|7879]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/P51149|P51149]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=RAB7A&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20RAB7A|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/602298|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**:  RAB family members are small, RAS-related GTP-binding proteins that are important regulators of vesicular transport. Each RAB protein targets multiple proteins that act in exocytic / endocytic pathways. This gene encodes a RAB family member that regulates vesicle traffic in the late endosomes and also from late endosomes to lysosomes. This encoded protein is also involved in the cellular vacuolation of the VacA cytotoxin of Helicobacter pylori. Mutations at highly conserved amino acid residues in this gene have caused some forms of Charcot-Marie-Tooth (CMT) type 2 neuropathies. [provided by RefSeq, Jul 2008]. 
  * **<color #00a2e8>UniProt Summary</color>**:  Key regulator in endo-lysosomal trafficking. Governs early-to-late endosomal maturation, microtubule minus-end as well as plus-end directed endosomal migration and positioning, and endosome-lysosome transport through different protein-protein interaction cascades. Plays a central role, not only in endosomal traffic, but also in many other cellular and physiological events, such as growth-factor-mediated cell signaling, nutrient- transportor mediated nutrient uptake, neurotrophin transport in the axons of neurons and lipid metabolism. Also involved in regulation of some specialized endosomal membrane trafficking, such as maturation of melanosomes, pathogen-induced phagosomes (or vacuoles) and autophagosomes. Plays a role in the maturation and acidification of phagosomes that engulf pathogens, such as S.aureus and M.tuberculosis. Plays a role in the fusion of phagosomes with lysosomes. Plays important roles in microbial pathogen infection and survival, as well as in participating in the life cycle of viruses. Microbial pathogens possess survival strategies governed by RAB7A, sometimes by employing RAB7A function (e.g. Salmonella) and sometimes by excluding RAB7A function (e.g. Mycobacterium). In concert with RAC1, plays a role in regulating the formation of RBs (ruffled borders) in osteoclasts. Controls the endosomal trafficking and neurite outgrowth signaling of NTRK1/TRKA (PubMed:11179213, PubMed:12944476, PubMed:14617358, PubMed:20028791, PubMed:21255211). Regulates the endocytic trafficking of the EGF- EGFR complex by regulating its lysosomal degradation. Involved in the ADRB2-stimulated lipolysis through lipophagy, a cytosolic lipase-independent autophagic pathway (By similarity). Required for the exosomal release of SDCBP, CD63 and syndecan (PubMed:22660413). {ECO:0000250|UniProtKB:P51150, ECO:0000269|PubMed:11179213, ECO:0000269|PubMed:12944476, ECO:0000269|PubMed:14617358, ECO:0000269|PubMed:20028791, ECO:0000269|PubMed:22660413}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|Gtr1 RagA|
|Ras|
|Arf|
|Miro|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|extrinsic component of lysosome membrane|
|lipophagy|
|negative regulation of intralumenal vesicle formation|
|negative regulation of endosome organization|
|epidermal growth factor catabolic process|
|regulation of intralumenal vesicle formation|
|negative regulation of exosomal secretion|
|regulation of endosome organization|
|protein to membrane docking|
|retromer complex binding|
|exit from host cell|
|viral release from host cell|
|exit from host|
|movement in environment of other organism involved in symbiotic interaction|
|movement in host environment|
|multi-organism cellular localization|
|multi-organism intracellular transport|
|phagosome-lysosome fusion|
|phagolysosome assembly|
|melanosome membrane|
|positive regulation of exosomal secretion|
|phagophore assembly site membrane|
|regulation of exosomal secretion|
|retromer complex|
|protein targeting to lysosome|
|selective autophagy|
|early endosome to late endosome transport|
|vesicle-mediated transport between endosomal compartments|
|vacuolar membrane|
|phagosome acidification|
|autophagosome membrane|
|negative regulation of exocytosis|
|protein targeting to vacuole|
|protein localization to lysosome|
|phagosome maturation|
|intracellular pH reduction|
|negative regulation of epidermal growth factor receptor signaling pathway|
|establishment of protein localization to vacuole|
|pH reduction|
|negative regulation of ERBB signaling pathway|
|endosome to lysosome transport|
|lysosome organization|
|lytic vacuole organization|
|phagocytic vesicle|
|protein localization to vacuole|
|autophagosome assembly|
|autophagosome organization|
|multi-organism transport|
|multi-organism localization|
|vesicle fusion|
|Rab protein signal transduction|
|phagocytic vesicle membrane|
|GDP binding|
|organelle membrane fusion|
|lipid droplet|
|regulation of epidermal growth factor receptor signaling pathway|
|regulation of intracellular pH|
|positive regulation of exocytosis|
|regulation of ERBB signaling pathway|
|regulation of cellular pH|
|retrograde transport, endosome to Golgi|
|organelle fusion|
|secretory granule membrane|
|antigen processing and presentation of exogenous peptide antigen via MHC class II|
|regulation of pH|
|antigen processing and presentation of peptide antigen via MHC class II|
|antigen processing and presentation of peptide or polysaccharide antigen via MHC class II|
|lysosomal transport|
|positive regulation of viral process|
|cellular monovalent inorganic cation homeostasis|
|late endosome membrane|
|late endosome|
|vacuole organization|
|membrane fusion|
|monovalent inorganic cation homeostasis|
|vacuolar transport|
|cytosolic transport|
|interaction with host|
|macroautophagy|
|antigen processing and presentation of exogenous peptide antigen|
|membrane docking|
|antigen processing and presentation of exogenous antigen|
|antigen processing and presentation of peptide antigen|
|viral life cycle|
|negative regulation of secretion by cell|
|regulation of viral process|
|endosome membrane|
|antigen processing and presentation|
|positive regulation of protein catabolic process|
|regulation of symbiosis, encompassing mutualism through parasitism|
|regulation of exocytosis|
|endosomal transport|
|negative regulation of secretion|
|Ras protein signal transduction|
|lysosome|
|autophagy|
|process utilizing autophagic mechanism|
|lipid catabolic process|
|vesicle organization|
|lysosomal membrane|
|small GTPase mediated signal transduction|
|phagocytosis|
|GTPase activity|
|protein targeting|
|negative regulation of organelle organization|
|regulation of protein catabolic process|
|GTP binding|
|positive regulation of secretion by cell|
|establishment of protein localization to organelle|
|positive regulation of catabolic process|
|positive regulation of secretion|
|negative regulation of transport|
|neutrophil degranulation|
|neutrophil activation involved in immune response|
|neutrophil mediated immunity|
|neutrophil activation|
|granulocyte activation|
|positive regulation of multi-organism process|
|leukocyte degranulation|
|myeloid leukocyte mediated immunity|
|positive regulation of cellular component biogenesis|
|myeloid cell activation involved in immune response|
|regulation of vesicle-mediated transport|
|endocytosis|
|myeloid leukocyte activation|
|cellular protein catabolic process|
|leukocyte activation involved in immune response|
|cell activation involved in immune response|
|cellular cation homeostasis|
|cellular ion homeostasis|
|import into cell|
|protein catabolic process|
|viral process|
|regulated exocytosis|
|negative regulation of cellular component organization|
|cation homeostasis|
|inorganic ion homeostasis|
|protein localization to organelle|
|cellular chemical homeostasis|
|regulation of secretion by cell|
|organelle assembly|
|leukocyte mediated immunity|
|regulation of multi-organism process|
|symbiotic process|
|ion homeostasis|
|exocytosis|
|regulation of secretion|
|interspecies interaction between organisms|
|membrane organization|
|cellular homeostasis|
|cellular macromolecule catabolic process|
|leukocyte activation|
|regulation of cellular component biogenesis|
|Golgi apparatus|
|positive regulation of transport|
|intracellular protein transport|
|regulation of catabolic process|
|secretion by cell|
|export from cell|
|macromolecule catabolic process|
|organonitrogen compound catabolic process|
|cell activation|
|immune effector process|
|chemical homeostasis|
|secretion|
|lipid metabolic process|
|negative regulation of signal transduction|
|regulation of organelle organization|
|locomotion|
|negative regulation of cell communication|
|negative regulation of signaling|
|protein transport|
|intracellular transport|
|peptide transport|
|amide transport|
|cellular protein localization|
|cellular macromolecule localization|
|establishment of protein localization|
|negative regulation of response to stimulus|
|homeostatic process|
|intracellular signal transduction|
|positive regulation of protein metabolic process|
|organic substance catabolic process|
|cellular catabolic process|
|establishment of localization in cell|
|nitrogen compound transport|
|regulation of transport|
|immune response|
|vesicle-mediated transport|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp344|Chlorpromazine 10μM R07 exp344]]|-3.11|
|[[:results:exp164|Q15 1 to 2μM on day4 R04 exp164]]|-3.05|
|[[:results:exp169|BH1 1μM R04 exp169]]|-3.01|
|[[:results:exp508|NN-Dimethylsphingosine 2.5μM R08 exp508]]|-2.92|
|[[:results:exp490|Hydroxychloroquine 30μM R08 exp490]]|-2.88|
|[[:results:exp442|Ibrutinib 10μM R08 exp442]]|-2.83|
|[[:results:exp177|Apcin 25μM plus proTAME 2μM R04 exp177]]|-2.74|
|[[:results:exp470|Chloroquine 32μM R08 exp470]]|-2.67|
|[[:results:exp182|IU1-47 25μM R04 exp182]]|-2.66|
|[[:results:exp422|Wiskostatin 3μM R07 exp422]]|-2.52|
|[[:results:exp68|Clomiphene 4.4μM R02 exp68]]|-2.38|
|[[:results:exp77|Prochlorperazine 5.2μM R02 exp77]]|-2.37|
|[[:results:exp400|Senexin-A 25μM R07 exp400]]|-2.35|
|[[:results:exp483|FTY720 3μM R08 exp483]]|-2.33|
|[[:results:exp492|iCRT14 30μM R08 exp492]]|-2.27|
|[[:results:exp356|Docosahexaenoic-acid 50μM R07 exp356]]|-2.26|
|[[:results:exp454|Bafilomycin-A1 0.009μM R08 exp454]]|-2.18|
|[[:results:exp187|proTAME 5μM R04 exp187]]|-2.08|
|[[:results:exp79|Q15 2.7μM R02 exp79]]|-2.07|
|[[:results:exp176|Apcin 50 to 100μM on day4 R04 exp176]]|-2.05|
|[[:results:exp112|S-DABN 8μM R03 exp112]]|-2.04|
|[[:results:exp218|A-395 10μM R05 exp218]]|-2.03|
|[[:results:exp416|Tubacin 1.6μM R07 exp416]]|-2.03|
|[[:results:exp530|Thioridazine 5μM R08 exp530]]|-2.02|
|[[:results:exp321|ABT-702 5μM plus Deferoxamine 11μM R07 exp321]]|-1.93|
|[[:results:exp227|Cryptotanshinone 12μM R05 exp227]]|-1.92|
|[[:results:exp450|Artemisinin 50μM R08 exp450]]|-1.9|
|[[:results:exp326|CCT251545 20μM R07 exp326]]|-1.89|
|[[:results:exp461|BS-181 20μM R08 exp461]]|-1.88|
|[[:results:exp480|ETC-159 50μM R08 exp480]]|-1.88|
|[[:results:exp434|Vemurafenib 6.6μM R08 exp434]]|-1.86|
|[[:results:exp89|Vemurafenib 6.6μM R02 exp89]]|-1.83|
|[[:results:exp32|Rifampicin 10μM R00 exp32]]|-1.73|
|[[:results:exp282|Fluvastatin 2.2μM R06 exp282]]|1.84|
|[[:results:exp536|Vitamin-D3 40μM R08 exp536]]|1.95|
|[[:results:exp226|Cerivastatin 0.15μM R05 exp226]]|3.49|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:c:ccdc22|CCDC22]]|0.51|
|[[:human genes:v:vps29|VPS29]]|0.506|
|[[:human genes:c:commd3|COMMD3]]|0.499|
|[[:human genes:c:ccdc53|CCDC53]]|0.481|
|[[:human genes:c:commd5|COMMD5]]|0.471|
|[[:human genes:a:arpc4|ARPC4]]|0.465|
|[[:human genes:a:actr3|ACTR3]]|0.457|
|[[:human genes:l:ldlr|LDLR]]|0.456|
|[[:human genes:c:commd10|COMMD10]]|0.445|
|[[:human genes:k:kiaa0196|KIAA0196]]|0.435|
|[[:human genes:a:arpc2|ARPC2]]|0.423|
|[[:human genes:k:kiaa1033|KIAA1033]]|0.406|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 5/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|0/28|
|blood|0/28|
|bone|0/26|
|breast|0/33|
|central nervous system|1/56|
|cervix|0/4|
|colorectal|0/17|
|esophagus|0/13|
|fibroblast|0/1|
|gastric|0/16|
|kidney|1/21|
|liver|0/20|
|lung|1/75|
|lymphocyte|0/16|
|ovary|0/26|
|pancreas|0/24|
|peripheral nervous system|0/16|
|plasma cell|0/15|
|prostate|0/1|
|skin|1/24|
|soft tissue|0/9|
|thyroid|0/2|
|upper aerodigestive|0/22|
|urinary tract|0/29|
|uterus|0/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 9973
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 6.74 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='RAB7A Expression in NALM6 Cells: 6.74'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>