RAD1 [The Human Chemical-Genetic Interaction Map Project]

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======= RAD1 =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: RAD1
  * **<color #00a2e8>Official Name</color>**: RAD1 checkpoint DNA exonuclease
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=5810|5810]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/O60671|O60671]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=RAD1&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20RAD1|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/603153|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**: N/A
  * **<color #00a2e8>UniProt Summary</color>**:  Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair (PubMed:10846170, PubMed:10884395). The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex (PubMed:12578958). Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER) (PubMed:15871698). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates (PubMed:15314187, PubMed:15556996, PubMed:15871698). The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase (PubMed:21659603). Isoform 1 possesses 3'->5' double stranded DNA exonuclease activity (PubMed:9660799). {ECO:0000269|PubMed:10846170, ECO:0000269|PubMed:10884395, ECO:0000269|PubMed:12578958, ECO:0000269|PubMed:15314187, ECO:0000269|PubMed:15556996, ECO:0000269|PubMed:15871698, ECO:0000269|PubMed:21659603, ECO:0000269|PubMed:9660799}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|Rad1|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|meiotic recombination checkpoint|
|exodeoxyribonuclease III activity|
|checkpoint clamp complex|
|meiotic cell cycle checkpoint|
|negative regulation of meiotic nuclear division|
|negative regulation of meiotic cell cycle|
|3-5 exonuclease activity|
|regulation of meiotic nuclear division|
|substantia nigra development|
|negative regulation of nuclear division|
|regulation of meiotic cell cycle|
|negative regulation of reproductive process|
|damaged DNA binding|
|neural nucleus development|
|cellular response to ionizing radiation|
|midbrain development|
|chromosome|
|DNA damage checkpoint|
|DNA integrity checkpoint|
|response to ionizing radiation|
|regulation of reproductive process|
|meiotic cell cycle process|
|cellular response to radiation|
|regulation of signal transduction by p53 class mediator|
|regulation of nuclear division|
|cell cycle checkpoint|
|DNA replication|
|meiotic cell cycle|
|nucleic acid phosphodiester bond hydrolysis|
|cellular response to abiotic stimulus|
|cellular response to environmental stimulus|
|negative regulation of cell cycle process|
|negative regulation of organelle organization|
|response to radiation|
|DNA repair|
|negative regulation of cell cycle|
|intracellular membrane-bounded organelle|
|negative regulation of cellular component organization|
|brain development|
|DNA metabolic process|
|regulation of cell cycle process|
|cellular response to DNA damage stimulus|
|head development|
|central nervous system development|
|cell cycle process|
|response to abiotic stimulus|
|regulation of cell cycle|
|regulation of organelle organization|
|cell cycle|
|reproductive process|
|reproduction|
|cellular response to stress|
|cellular macromolecule biosynthetic process|
|macromolecule biosynthetic process|
|regulation of intracellular signal transduction|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp392|PT-1 25μM R07 exp392]]|-2.5|
|[[:results:exp238|Parthenolide 2.5μM R05 exp238]]|-2.13|
|[[:results:exp520|Rucaparib 6.5μM R08 exp520]]|-2.12|
|[[:results:exp78|Pterostilbene 16μM R02 exp78]]|-2.11|
|[[:results:exp121|Golgicide-A 2μM R03 exp121]]|-2.07|
|[[:results:exp224|CB-839 10μM R05 exp224]]|-2.06|
|[[:results:exp329|Hydroxyurea 100μM R07 exp329]]|-2|
|[[:results:exp234|Ethanol 0.01 R05 exp234]]|-1.88|
|[[:results:exp228|Demecolcine 0.03μM R05 exp228]]|-1.78|
|[[:results:exp335|Aminopterin 0.005μM R07 exp335]]|-1.77|
|[[:results:exp444|THZ531 0.225μM R08 exp444]]|-1.77|
|[[:results:exp243|S-trityl-L-cysteine 0.5μM R05 exp243]]|-1.76|
|[[:results:exp1|5-Fluorouracil 2μM R00 exp1]]|-1.75|
|[[:results:exp232|Epothilone-D 0.004 to 0.006μM on day4 R05 exp232]]|-1.7|
|[[:results:exp521|Salinomycin 3.5μM R08 exp521]]|1.79|
|[[:results:exp401|SNS-032 25μM R07 exp401]]|2.05|
|[[:results:exp17|DABN 20μM R00 exp17]]|2.44|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:t:trmt5|TRMT5]]|0.527|
|[[:human genes:d:dpagt1|DPAGT1]]|0.515|
|[[:human genes:r:rpe|RPE]]|0.513|
|[[:human genes:g:gtf3c2|GTF3C2]]|0.511|
|[[:human genes:a:adsl|ADSL]]|0.506|
|[[:human genes:h:hars|HARS]]|0.502|
|[[:human genes:r:rpp21|RPP21]]|0.501|
|[[:human genes:v:vrk1|VRK1]]|0.5|
|[[:human genes:a:alg1|ALG1]]|0.497|
|[[:human genes:p:pmm2|PMM2]]|0.486|
|[[:human genes:g:gmppb|GMPPB]]|0.485|
|[[:human genes:n:nsmce1|NSMCE1]]|0.484|
|[[:human genes:o:otud5|OTUD5]]|0.478|
|[[:human genes:t:triap1|TRIAP1]]|0.477|
|[[:human genes:t:tmem41b|TMEM41B]]|0.471|
|[[:human genes:e:eno1|ENO1]]|0.47|
|[[:human genes:g:gemin5|GEMIN5]]|0.469|
|[[:human genes:s:srp14|SRP14]]|0.468|
|[[:human genes:h:hspd1|HSPD1]]|0.467|
|[[:human genes:r:rtcb|RTCB]]|0.466|
|[[:human genes:g:gtf3c3|GTF3C3]]|0.458|
|[[:human genes:n:nae1|NAE1]]|0.458|
|[[:human genes:t:txnl4b|TXNL4B]]|0.456|
|[[:human genes:v:vezt|VEZT]]|0.455|
|[[:human genes:u:utp23|UTP23]]|0.453|
|[[:human genes:r:rnmt|RNMT]]|0.452|
|[[:human genes:u:uap1|UAP1]]|0.449|
|[[:human genes:p:polr3h|POLR3H]]|0.449|
|[[:human genes:t:tbca|TBCA]]|0.448|
|[[:human genes:t:tti2|TTI2]]|0.448|
|[[:human genes:g:gfpt1|GFPT1]]|0.448|
|[[:human genes:g:gtf3c4|GTF3C4]]|0.447|
|[[:human genes:g:gnb1l|GNB1L]]|0.447|
|[[:human genes:g:gemin8|GEMIN8]]|0.446|
|[[:human genes:z:zrsr2|ZRSR2]]|0.443|
|[[:human genes:a:alg5|ALG5]]|0.441|
|[[:human genes:t:tsen54|TSEN54]]|0.438|
|[[:human genes:t:thg1l|THG1L]]|0.437|
|[[:human genes:z:znf259|ZNF259]]|0.435|
|[[:human genes:n:nars|NARS]]|0.434|
|[[:human genes:y:yars|YARS]]|0.434|
|[[:human genes:a:atxn10|ATXN10]]|0.429|
|[[:human genes:d:ddx20|DDX20]]|0.429|
|[[:human genes:p:pik3r4|PIK3R4]]|0.428|
|[[:human genes:s:seh1l|SEH1L]]|0.428|
|[[:human genes:d:dad1|DAD1]]|0.426|
|[[:human genes:s:srp68|SRP68]]|0.421|
|[[:human genes:r:rptor|RPTOR]]|0.421|
|[[:human genes:c:cct7|CCT7]]|0.42|
|[[:human genes:r:rabggtb|RABGGTB]]|0.42|
|[[:human genes:r:rbm14|RBM14]]|0.418|
|[[:human genes:o:osgep|OSGEP]]|0.418|
|[[:human genes:g:gtf3c6|GTF3C6]]|0.415|
|[[:human genes:p:psmg4|PSMG4]]|0.413|
|[[:human genes:f:fnta|FNTA]]|0.413|
|[[:human genes:a:aars|AARS]]|0.412|
|[[:human genes:c:chchd4|CHCHD4]]|0.41|
|[[:human genes:u:uhrf1|UHRF1]]|0.41|
|[[:human genes:e:eif4e|EIF4E]]|0.41|
|[[:human genes:l:lamtor5|LAMTOR5]]|0.409|
|[[:human genes:s:spr|SPR]]|0.409|
|[[:human genes:t:timm22|TIMM22]]|0.408|
|[[:human genes:a:alg13|ALG13]]|0.407|
|[[:human genes:t:tti1|TTI1]]|0.407|
|[[:human genes:h:haus8|HAUS8]]|0.406|
|[[:human genes:u:ube2j1|UBE2J1]]|0.405|
|[[:human genes:a:atic|ATIC]]|0.404|
|[[:human genes:t:tprkb|TPRKB]]|0.402|
|[[:human genes:p:pelo|PELO]]|0.4|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 4/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|0/28|
|blood|0/28|
|bone|0/26|
|breast|0/33|
|central nervous system|0/56|
|cervix|0/4|
|colorectal|0/17|
|esophagus|0/13|
|fibroblast|0/1|
|gastric|0/16|
|kidney|1/21|
|liver|0/20|
|lung|0/75|
|lymphocyte|0/16|
|ovary|0/26|
|pancreas|0/24|
|peripheral nervous system|1/16|
|plasma cell|0/15|
|prostate|0/1|
|skin|0/24|
|soft tissue|0/9|
|thyroid|0/2|
|upper aerodigestive|0/22|
|urinary tract|1/29|
|uterus|0/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 2781
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 6.17 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='RAD1 Expression in NALM6 Cells: 6.17'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>