RAD9A [The Human Chemical-Genetic Interaction Map Project]

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======= RAD9A =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: RAD9A
  * **<color #00a2e8>Official Name</color>**: RAD9 checkpoint clamp component A
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=5883|5883]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/Q99638|Q99638]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=RAD9A&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20RAD9A|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/603761|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**: N/A
  * **<color #00a2e8>UniProt Summary</color>**:  Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase. RAD9A possesses 3'->5' double stranded DNA exonuclease activity. Its phosphorylation by PRKCD may be required for the formation of the 9-1-1 complex. {ECO:0000269|PubMed:10713044, ECO:0000269|PubMed:21659603}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|Rad9|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|exodeoxyribonuclease III activity|
|checkpoint clamp complex|
|positive regulation of intrinsic apoptotic signaling pathway in response to DNA damage|
|intra-S DNA damage checkpoint|
|DNA replication checkpoint|
|3-5 exonuclease activity|
|regulation of intrinsic apoptotic signaling pathway in response to DNA damage|
|positive regulation of intrinsic apoptotic signaling pathway|
|cellular response to ionizing radiation|
|mitotic DNA damage checkpoint|
|positive regulation of response to DNA damage stimulus|
|mitotic DNA integrity checkpoint|
|histone deacetylase binding|
|SH3 domain binding|
|DNA damage checkpoint|
|DNA integrity checkpoint|
|response to ionizing radiation|
|mitotic cell cycle checkpoint|
|regulation of intrinsic apoptotic signaling pathway|
|positive regulation of apoptotic signaling pathway|
|cellular response to radiation|
|regulation of signal transduction by p53 class mediator|
|cell cycle checkpoint|
|DNA replication|
|regulation of response to DNA damage stimulus|
|nucleic acid phosphodiester bond hydrolysis|
|negative regulation of mitotic cell cycle|
|cellular response to environmental stimulus|
|cellular response to abiotic stimulus|
|enzyme binding|
|regulation of apoptotic signaling pathway|
|response to radiation|
|protein kinase binding|
|DNA repair|
|negative regulation of cell cycle|
|mitotic cell cycle process|
|regulation of mitotic cell cycle|
|positive regulation of apoptotic process|
|positive regulation of programmed cell death|
|mitotic cell cycle|
|positive regulation of cell death|
|regulation of cellular response to stress|
|DNA metabolic process|
|cellular response to DNA damage stimulus|
|cell cycle process|
|positive regulation of intracellular signal transduction|
|response to abiotic stimulus|
|regulation of cell cycle|
|cell cycle|
|regulation of response to stress|
|regulation of apoptotic process|
|regulation of programmed cell death|
|positive regulation of signal transduction|
|regulation of cell death|
|cellular response to stress|
|cellular macromolecule biosynthetic process|
|macromolecule biosynthetic process|
|positive regulation of cell communication|
|positive regulation of signaling|
|regulation of intracellular signal transduction|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='default' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp457|Bisphenol F 50μM R08 exp457]]|-2.09|
|[[:results:exp501|Methotrexate 0.01μM R08 exp501]]|-1.86|
|[[:results:exp446|4-Nitroquinoline-1-oxide 0.5μM R08 exp446]]|-1.85|
|[[:results:exp529|Thimerosal 0.85μM R08 exp529]]|-1.8|
|[[:results:exp334|All-trans-Retinoic-Acid 40μM R07 exp334]]|-1.79|
|[[:results:exp329|Hydroxyurea 100μM R07 exp329]]|-1.71|
|[[:results:exp419|Tunicamycin 0.04 to 0.075μM  on day4 R07 exp419]]|-1.7|
|[[:results:exp142|OICR-9429 10μM R03 exp142]]|2.04|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
No correlation found to any other genes in chemogenomics.
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 550/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|1/1|
|bile duct|20/28|
|blood|23/28|
|bone|16/26|
|breast|22/33|
|central nervous system|33/56|
|cervix|2/4|
|colorectal|9/17|
|esophagus|8/13|
|fibroblast|1/1|
|gastric|12/16|
|kidney|18/21|
|liver|14/20|
|lung|53/75|
|lymphocyte|12/16|
|ovary|23/26|
|pancreas|10/24|
|peripheral nervous system|12/16|
|plasma cell|12/15|
|prostate|0/1|
|skin|19/24|
|soft tissue|7/9|
|thyroid|1/2|
|upper aerodigestive|17/22|
|urinary tract|25/29|
|uterus|4/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 735
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 5.65 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='RAD9A Expression in NALM6 Cells: 5.65'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>