RBBP8 [The Human Chemical-Genetic Interaction Map Project]

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======= RBBP8 =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: RBBP8
  * **<color #00a2e8>Official Name</color>**: RB binding protein 8, endonuclease
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=5932|5932]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/Q99708|Q99708]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=RBBP8&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20RBBP8|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/604124|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**:  The protein encoded by this gene is a ubiquitously expressed nuclear protein. It is found among several proteins that bind directly to retinoblastoma protein, which regulates cell proliferation. This protein complexes with transcriptional co-repressor CTBP. It is also associated with BRCA1 and is thought to modulate the functions of BRCA1 in transcriptional regulation, DNA repair, and/or cell cycle checkpoint control. It is suggested that this gene may itself be a tumor suppressor acting in the same pathway as BRCA1. Three transcript variants encoding two different isoforms have been found for this gene. More transcript variants exist, but their full-length natures have not been determined. [provided by RefSeq, Jul 2008]. 
  * **<color #00a2e8>UniProt Summary</color>**:  Endonuclease that cooperates with the MRE11-RAD50-NBN (MRN) complex in DNA-end resection, the first step of double- strand break (DSB) repair through the homologous recombination (HR) pathway. HR is restricted to S and G2 phases of the cell cycle and preferentially repairs DSBs resulting from replication fork collapse. Key determinant of DSB repair pathway choice, as it commits cells to HR by preventing classical non-homologous end- joining (NHEJ). Functions downstream of the MRN complex and ATM, promotes ATR activation and its recruitment to DSBs in the S/G2 phase facilitating the generation of ssDNA. Component of the BRCA1-RBBP8 complex that regulates CHEK1 activation and controls cell cycle G2/M checkpoints on DNA damage (PubMed:10764811, PubMed:10910365, PubMed:15485915, PubMed:16581787, PubMed:16818604, PubMed:17965729, PubMed:19202191, PubMed:19759395, PubMed:20064462, PubMed:20829486). During immunoglobulin heavy chain class-switch recombination, promotes microhomology-mediated alternative end joining (A-NHEJ) and plays an essential role in chromosomal translocations (By similarity). {ECO:0000250|UniProtKB:Q80YR6, ECO:0000269|PubMed:10764811, ECO:0000269|PubMed:10910365, ECO:0000269|PubMed:15485915, ECO:0000269|PubMed:16581787, ECO:0000269|PubMed:16818604, ECO:0000269|PubMed:17965729, ECO:0000269|PubMed:19202191, ECO:0000269|PubMed:19759395, ECO:0000269|PubMed:20064462, ECO:0000269|PubMed:20829486}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|CtIP N|
|SAE2|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|double-strand/single-strand DNA junction binding|
|flap-structured DNA binding|
|DNA double-strand break processing involved in repair via single-strand annealing|
|Y-form DNA binding|
|double-strand break repair via single-strand annealing|
|single-stranded DNA endodeoxyribonuclease activity|
|DNA double-strand break processing|
|hatching|
|organism emergence from protective structure|
|blastocyst hatching|
|RNA polymerase II repressing transcription factor binding|
|negative regulation of G0 to G1 transition|
|regulation of G0 to G1 transition|
|transcriptional repressor complex|
|damaged DNA binding|
|site of double-strand break|
|non-recombinational repair|
|double-strand break repair via homologous recombination|
|blastocyst development|
|recombinational repair|
|double-stranded DNA binding|
|single-stranded DNA binding|
|nucleotide-excision repair|
|G1/S transition of mitotic cell cycle|
|cell cycle G1/S phase transition|
|response to estradiol|
|regulation of signal transduction by p53 class mediator|
|double-strand break repair|
|DNA replication|
|DNA recombination|
|meiotic cell cycle|
|transcription corepressor activity|
|mitotic cell cycle phase transition|
|cell cycle phase transition|
|nucleic acid phosphodiester bond hydrolysis|
|negative regulation of cell cycle process|
|in utero embryonic development|
|cell division|
|DNA repair|
|negative regulation of cell cycle|
|mitotic cell cycle process|
|chordate embryonic development|
|embryo development ending in birth or egg hatching|
|mitotic cell cycle|
|intracellular membrane-bounded organelle|
|DNA metabolic process|
|regulation of cell cycle process|
|cellular response to DNA damage stimulus|
|response to lipid|
|response to hormone|
|response to organic cyclic compound|
|embryo development|
|cell cycle process|
|identical protein binding|
|regulation of cell cycle|
|negative regulation of nucleic acid-templated transcription|
|negative regulation of RNA biosynthetic process|
|negative regulation of RNA metabolic process|
|cell cycle|
|reproductive process|
|reproduction|
|negative regulation of nucleobase-containing compound metabolic process|
|negative regulation of macromolecule biosynthetic process|
|response to endogenous stimulus|
|negative regulation of cellular biosynthetic process|
|negative regulation of biosynthetic process|
|response to oxygen-containing compound|
|cellular response to stress|
|cellular macromolecule biosynthetic process|
|macromolecule biosynthetic process|
|regulation of intracellular signal transduction|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='default' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp356|Docosahexaenoic-acid 50μM R07 exp356]]|1.71|
|[[:results:exp180|Dynasore 10μM R04 exp180]]|1.71|
|[[:results:exp114|A-196 10μM R03 exp114]]|1.74|
|[[:results:exp147|Resveratrol 16μM R03 exp147]]|1.8|
|[[:results:exp132|Metformin 40μM R03 exp132]]|1.96|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
No correlation found to any other genes in chemogenomics.
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 376/726
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|1/1|
|909776.0|1/1|
|bile duct|15/28|
|blood|12/28|
|bone|14/25|
|breast|14/33|
|central nervous system|27/56|
|cervix|0/4|
|colorectal|9/17|
|esophagus|6/13|
|fibroblast|0/1|
|gastric|5/15|
|kidney|12/21|
|liver|11/20|
|lung|39/75|
|lymphocyte|8/14|
|ovary|11/26|
|pancreas|16/24|
|peripheral nervous system|13/16|
|plasma cell|4/15|
|prostate|0/1|
|skin|21/24|
|soft tissue|2/7|
|thyroid|1/2|
|upper aerodigestive|11/22|
|urinary tract|15/29|
|uterus|2/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 2488
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 6.79 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='RBBP8 Expression in NALM6 Cells: 6.79'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>