RFWD3 [The Human Chemical-Genetic Interaction Map Project]

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======= RFWD3 =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: RFWD3
  * **<color #00a2e8>Official Name</color>**: ring finger and WD repeat domain 3
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=55159|55159]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/Q6PCD5|Q6PCD5]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=RFWD3&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20RFWD3|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/614151|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**: N/A
  * **<color #00a2e8>UniProt Summary</color>**:  E3 ubiquitin-protein ligase required for the repair of DNA interstrand cross-links (ICL) in response to DNA damage (PubMed:21504906, PubMed:21558276, PubMed:26474068, PubMed:28575657, PubMed:28575658). Plays a key role in RPA- mediated DNA damage signaling and repair (PubMed:21504906, PubMed:21558276, PubMed:26474068, PubMed:28575657, PubMed:28575658). Acts by mediating ubiquitination of the RPA complex (RPA1, RPA2 and RPA3 subunits) and RAD51 at stalled replication forks, leading to remove them from DNA damage sites and promote homologous recombination (PubMed:26474068, PubMed:28575657, PubMed:28575658). Also mediates the ubiquitination of p53/TP53 in the late response to DNA damage, and acts as a positive regulator of p53/TP53 stability, thereby regulating the G1/S DNA damage checkpoint (PubMed:20173098). May act by catalyzing the formation of short polyubiquitin chains on p53/TP53 that are not targeted to the proteasome (PubMed:20173098). In response to ionizing radiation, interacts with MDM2 and enhances p53/TP53 ubiquitination, possibly by restricting MDM2 from extending polyubiquitin chains on ubiquitinated p53/TP53 (PubMed:20173098). {ECO:0000269|PubMed:20173098, ECO:0000269|PubMed:21504906, ECO:0000269|PubMed:21558276, ECO:0000269|PubMed:26474068, ECO:0000269|PubMed:28575657, ECO:0000269|PubMed:28575658}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|zf-rbx1|
|zf-C3HC4|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|chromosome breakage|
|programmed DNA elimination|
|MDM2/MDM4 family protein binding|
|regulation of DNA damage checkpoint|
|site of DNA damage|
|regulation of cell cycle checkpoint|
|replication fork processing|
|DNA-dependent DNA replication maintenance of fidelity|
|interstrand cross-link repair|
|site of double-strand break|
|mitotic G1 DNA damage checkpoint|
|mitotic G1/S transition checkpoint|
|G1 DNA damage checkpoint|
|p53 binding|
|mitotic DNA damage checkpoint|
|double-strand break repair via homologous recombination|
|recombinational repair|
|PML body|
|negative regulation of G1/S transition of mitotic cell cycle|
|mitotic DNA integrity checkpoint|
|negative regulation of cell cycle G1/S phase transition|
|DNA-dependent DNA replication|
|DNA damage checkpoint|
|DNA integrity checkpoint|
|response to ionizing radiation|
|regulation of G1/S transition of mitotic cell cycle|
|mitotic cell cycle checkpoint|
|regulation of cell cycle G1/S phase transition|
|double-strand break repair|
|cell cycle checkpoint|
|negative regulation of mitotic cell cycle phase transition|
|DNA replication|
|regulation of response to DNA damage stimulus|
|DNA recombination|
|negative regulation of cell cycle phase transition|
|ubiquitin-protein transferase activity|
|negative regulation of mitotic cell cycle|
|negative regulation of cell cycle process|
|regulation of mitotic cell cycle phase transition|
|response to radiation|
|regulation of cell cycle phase transition|
|DNA repair|
|negative regulation of cell cycle|
|mitotic cell cycle process|
|regulation of mitotic cell cycle|
|mitotic cell cycle|
|protein ubiquitination|
|regulation of cellular response to stress|
|DNA metabolic process|
|regulation of cell cycle process|
|protein modification by small protein conjugation|
|cellular response to DNA damage stimulus|
|protein modification by small protein conjugation or removal|
|cell cycle process|
|chromosome organization|
|response to abiotic stimulus|
|regulation of cell cycle|
|cell cycle|
|regulation of response to stress|
|cellular response to stress|
|cellular macromolecule biosynthetic process|
|macromolecule biosynthetic process|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='default' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp42|BI-6727 0.001μM R01 exp42]]|-2.12|
|[[:results:exp115|A-366 10μM R03 exp115]]|2.15|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
No correlation found to any other genes in chemogenomics.
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 1/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|0/28|
|blood|0/28|
|bone|0/26|
|breast|0/33|
|central nervous system|0/56|
|cervix|0/4|
|colorectal|0/17|
|esophagus|0/13|
|fibroblast|0/1|
|gastric|0/16|
|kidney|0/21|
|liver|0/20|
|lung|0/75|
|lymphocyte|0/16|
|ovary|0/26|
|pancreas|0/24|
|peripheral nervous system|0/16|
|plasma cell|0/15|
|prostate|0/1|
|skin|0/24|
|soft tissue|0/9|
|thyroid|0/2|
|upper aerodigestive|0/22|
|urinary tract|0/29|
|uterus|0/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 2348
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 7.71 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='RFWD3 Expression in NALM6 Cells: 7.71'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>