ROCK2 [The Human Chemical-Genetic Interaction Map Project]

This page is read only. You can view the source, but not change it. Ask your administrator if you think this is wrong.
======= ROCK2 =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: ROCK2
  * **<color #00a2e8>Official Name</color>**: Rho associated coiled-coil containing protein kinase 2
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=9475|9475]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/O75116|O75116]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=ROCK2&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20ROCK2|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/604002|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**:  The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho. [provided by RefSeq, Jul 2008]. 
  * **<color #00a2e8>UniProt Summary</color>**:  Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of ADD1, BRCA2, CNN1, EZR, DPYSL2, EP300, MSN, MYL9/MLC2, NPM1, RDX, PPP1R12A and VIM. Phosphorylates SORL1 and IRF4. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Positively regulates the activation of p42/MAPK1-p44/MAPK3 and of p90RSK/RPS6KA1 during myogenic differentiation. Plays an important role in the timely initiation of centrosome duplication. Inhibits keratinocyte terminal differentiation. May regulate closure of the eyelids and ventral body wall through organization of actomyosin bundles. Plays a critical role in the regulation of spine and synaptic properties in the hippocampus. Plays an important role in generating the circadian rhythm of the aortic myofilament Ca(2+) sensitivity and vascular contractility by modulating the myosin light chain phosphorylation. {ECO:0000269|PubMed:10579722, ECO:0000269|PubMed:15699075, ECO:0000269|PubMed:16574662, ECO:0000269|PubMed:17015463, ECO:0000269|PubMed:19131646, ECO:0000269|PubMed:19997641, ECO:0000269|PubMed:21084279, ECO:0000269|PubMed:21147781}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|Rho Binding|
|Pkinase Tyr|
|Pkinase|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|negative regulation of protein localization to lysosome|
|Rho-dependent protein serine/threonine kinase activity|
|regulation of angiotensin-activated signaling pathway|
|regulation of protein localization to lysosome|
|regulation of connective tissue growth factor production|
|positive regulation of connective tissue growth factor production|
|positive regulation of connective tissue replacement|
|negative regulation of bicellular tight junction assembly|
|regulation of connective tissue replacement|
|negative regulation of myosin-light-chain-phosphatase activity|
|positive regulation of fibroblast growth factor production|
|regulation of myosin-light-chain-phosphatase activity|
|positive regulation of aspartic-type endopeptidase activity involved in amyloid precursor protein catabolic process|
|regulation of fibroblast growth factor production|
|positive regulation of aspartic-type peptidase activity|
|positive regulation of protein localization to early endosome|
|regulation of protein localization to early endosome|
|cellular response to testosterone stimulus|
|positive regulation of centrosome duplication|
|regulation of aspartic-type endopeptidase activity involved in amyloid precursor protein catabolic process|
|positive regulation of centrosome cycle|
|regulation of aspartic-type peptidase activity|
|positive regulation of protein localization to endosome|
|regulation of protein localization to endosome|
|regulation of cellular response to hypoxia|
|viral RNA genome replication|
|regulation of endothelial cell development|
|regulation of establishment of endothelial barrier|
|negative regulation of nitric oxide biosynthetic process|
|negative regulation of nitric oxide metabolic process|
|positive regulation of amyloid-beta formation|
|positive regulation of amyloid precursor protein catabolic process|
|regulation of bicellular tight junction assembly|
|regulation of establishment of cell polarity|
|GTP-Rho binding|
|tau-protein kinase activity|
|negative regulation of cell junction assembly|
|regulation of establishment or maintenance of cell polarity|
|response to angiotensin|
|viral genome replication|
|negative regulation of reactive oxygen species biosynthetic process|
|regulation of amyloid-beta formation|
|aortic valve morphogenesis|
|negative regulation of biomineral tissue development|
|negative regulation of biomineralization|
|mRNA destabilization|
|positive regulation of cardiac muscle hypertrophy|
|centrosome duplication|
|positive regulation of tissue remodeling|
|positive regulation of muscle hypertrophy|
|aortic valve development|
|cellular response to acetylcholine|
|response to acetylcholine|
|RNA destabilization|
|regulation of endothelial cell differentiation|
|regulation of amyloid precursor protein catabolic process|
|semi-lunar valve development|
|response to testosterone|
|regulation of keratinocyte differentiation|
|cortical actin cytoskeleton organization|
|regulation of centrosome duplication|
|tau protein binding|
|cortical cytoskeleton organization|
|positive regulation of mRNA catabolic process|
|response to ischemia|
|positive regulation of stress fiber assembly|
|heart valve morphogenesis|
|smooth muscle contraction|
|negative regulation of reactive oxygen species metabolic process|
|regulation of cardiac muscle hypertrophy|
|regulation of nitric oxide biosynthetic process|
|regulation of centrosome cycle|
|regulation of muscle hypertrophy|
|regulation of focal adhesion assembly|
|regulation of cell-substrate junction assembly|
|negative regulation of phosphoprotein phosphatase activity|
|heart valve development|
|positive regulation of actin filament bundle assembly|
|regulation of epidermal cell differentiation|
|negative regulation of protein dephosphorylation|
|vascular endothelial growth factor receptor signaling pathway|
|regulation of adherens junction organization|
|cytoplasmic ribonucleoprotein granule|
|mitotic cytokinesis|
|I-kappaB kinase/NF-kappaB signaling|
|regulation of tissue remodeling|
|Rho protein signal transduction|
|positive regulation of mRNA metabolic process|
|regulation of stress fiber assembly|
|peptidyl-threonine phosphorylation|
|centrosome cycle|
|regulation of reactive oxygen species biosynthetic process|
|regulation of muscle adaptation|
|regulation of epidermis development|
|ephrin receptor signaling pathway|
|regulation of cell junction assembly|
|regulation of actomyosin structure organization|
|peptidyl-threonine modification|
|cellular response to ketone|
|regulation of biomineral tissue development|
|regulation of biomineralization|
|microtubule organizing center organization|
|regulation of actin filament bundle assembly|
|cytoskeleton-dependent cytokinesis|
|positive regulation of endothelial cell migration|
|cytokinesis|
|negative regulation of angiogenesis|
|negative regulation of phosphatase activity|
|negative regulation of blood vessel morphogenesis|
|negative regulation of dephosphorylation|
|negative regulation of cellular protein localization|
|regulation of cell-matrix adhesion|
|actomyosin structure organization|
|regulation of circadian rhythm|
|negative regulation of vasculature development|
|regulation of phosphoprotein phosphatase activity|
|negative regulation of translation|
|cellular response to ammonium ion|
|regulation of nervous system process|
|regulation of blood vessel diameter|
|regulation of tube diameter|
|regulation of protein dephosphorylation|
|regulation of tube size|
|positive regulation of epithelial cell migration|
|regulation of epithelial cell differentiation|
|regulation of G protein-coupled receptor signaling pathway|
|negative regulation of cellular amide metabolic process|
|cytokine production|
|positive regulation of cellular amide metabolic process|
|regulation of endothelial cell migration|
|response to transforming growth factor beta|
|vascular process in circulatory system|
|positive regulation of endopeptidase activity|
|peptidyl-serine phosphorylation|
|regulation of phosphatase activity|
|regulation of reactive oxygen species metabolic process|
|regulation of mRNA stability|
|protein localization to plasma membrane|
|regulation of RNA stability|
|response to ammonium ion|
|regulation of microtubule cytoskeleton organization|
|structural molecule activity|
|positive regulation of peptidase activity|
|response to ketone|
|positive regulation of supramolecular fiber organization|
|peptidyl-serine modification|
|viral life cycle|
|regulation of mRNA catabolic process|
|regulation of dephosphorylation|
|regulation of cell-substrate adhesion|
|regulation of epithelial cell migration|
|positive regulation of cytoskeleton organization|
|regulation of microtubule-based process|
|protein localization to cell periphery|
|regulation of muscle system process|
|Ras protein signal transduction|
|muscle contraction|
|regulation of actin filament organization|
|rhythmic process|
|positive regulation of cell cycle process|
|regulation of angiogenesis|
|muscle system process|
|small GTPase mediated signal transduction|
|regulation of vasculature development|
|positive regulation of cellular protein localization|
|regulation of mRNA metabolic process|
|regulation of actin cytoskeleton organization|
|regulation of supramolecular fiber organization|
|regulation of translation|
|regulation of neurotransmitter levels|
|positive regulation of proteolysis|
|protein serine/threonine kinase activity|
|positive regulation of cellular catabolic process|
|cytoskeleton|
|positive regulation of cell cycle|
|regulation of actin filament-based process|
|blood circulation|
|response to peptide hormone|
|circulatory system process|
|cellular response to drug|
|regulation of cellular amide metabolic process|
|regulation of endopeptidase activity|
|positive regulation of catabolic process|
|regulation of peptidase activity|
|positive regulation of cytokine production|
|negative regulation of hydrolase activity|
|response to peptide|
|microtubule cytoskeleton organization|
|protein localization to membrane|
|centrosome|
|cell division|
|actin cytoskeleton organization|
|positive regulation of cell migration|
|transmembrane receptor protein tyrosine kinase signaling pathway|
|regulation of anatomical structure size|
|cellular response to lipid|
|positive regulation of cellular component biogenesis|
|positive regulation of cell motility|
|posttranscriptional regulation of gene expression|
|heart development|
|response to growth factor|
|positive regulation of cellular component movement|
|regulation of cellular protein localization|
|regulation of cytoskeleton organization|
|cellular response to organic cyclic compound|
|positive regulation of MAPK cascade|
|positive regulation of locomotion|
|negative regulation of phosphate metabolic process|
|negative regulation of phosphorus metabolic process|
|embryonic morphogenesis|
|actin filament-based process|
|regulation of system process|
|negative regulation of protein modification process|
|mitotic cell cycle process|
|cellular response to hormone stimulus|
|positive regulation of organelle organization|
|cellular response to nitrogen compound|
|microtubule-based process|
|regulation of cell adhesion|
|mitotic cell cycle|
|regulation of cytokine production|
|viral process|
|negative regulation of cellular component organization|
|enzyme linked receptor protein signaling pathway|
|regulation of proteolysis|
|regulation of cellular response to stress|
|regulation of cell cycle process|
|regulation of MAPK cascade|
|positive regulation of hydrolase activity|
|symbiotic process|
|negative regulation of catalytic activity|
|interspecies interaction between organisms|
|regulation of cellular catabolic process|
|regulation of cell migration|
|response to lipid|
|circulatory system development|
|peptidyl-amino acid modification|
|response to hormone|
|regulation of cell motility|
|regulation of cellular localization|
|response to organic cyclic compound|
|regulation of cell development|
|negative regulation of developmental process|
|regulation of cellular component biogenesis|
|protein phosphorylation|
|embryo development|
|regulation of locomotion|
|regulation of cellular component movement|
|regulation of catabolic process|
|cell cycle process|
|response to organonitrogen compound|
|positive regulation of protein phosphorylation|
|positive regulation of intracellular signal transduction|
|response to drug|
|regulation of protein localization|
|negative regulation of cellular protein metabolic process|
|cellular response to oxygen-containing compound|
|positive regulation of phosphorylation|
|regulation of anatomical structure morphogenesis|
|response to nitrogen compound|
|negative regulation of protein metabolic process|
|cytoskeleton organization|
|positive regulation of phosphate metabolic process|
|positive regulation of phosphorus metabolic process|
|negative regulation of molecular function|
|regulation of cell cycle|
|negative regulation of multicellular organismal process|
|positive regulation of cellular component organization|
|cellular response to endogenous stimulus|
|positive regulation of protein modification process|
|regulation of hydrolase activity|
|phosphorylation|
|regulation of organelle organization|
|G protein-coupled receptor signaling pathway|
|cell cycle|
|negative regulation of cellular macromolecule biosynthetic process|
|RNA binding|
|positive regulation of catalytic activity|
|regulation of protein phosphorylation|
|negative regulation of macromolecule biosynthetic process|
|response to endogenous stimulus|
|regulation of response to stress|
|ATP binding|
|negative regulation of cellular biosynthetic process|
|negative regulation of biosynthetic process|
|response to oxygen-containing compound|
|cellular protein localization|
|regulation of phosphorylation|
|cellular macromolecule localization|
|positive regulation of cellular protein metabolic process|
|positive regulation of signal transduction|
|intracellular signal transduction|
|positive regulation of protein metabolic process|
|negative regulation of gene expression|
|positive regulation of RNA metabolic process|
|positive regulation of multicellular organismal process|
|positive regulation of molecular function|
|regulation of phosphate metabolic process|
|regulation of phosphorus metabolic process|
|regulation of cell differentiation|
|positive regulation of cell communication|
|positive regulation of signaling|
|regulation of intracellular signal transduction|
|regulation of protein modification process|
|positive regulation of nucleobase-containing compound metabolic process|
|system process|
|positive regulation of gene expression|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='default' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp243|S-trityl-L-cysteine 0.5μM R05 exp243]]|-3.19|
|[[:results:exp238|Parthenolide 2.5μM R05 exp238]]|-2.55|
|[[:results:exp210|LB-100 2μM R05 exp210]]|-2.27|
|[[:results:exp237|NN-Diethyl-meta-toluamide 100μM R05 exp237]]|-2.15|
|[[:results:exp220|BAY-598 4μM R05 exp220]]|-2.07|
|[[:results:exp437|Ethanol 0.003 R08 exp437]]|-2.02|
|[[:results:exp260|ABT-702 0.1μM R06 exp260]]|-1.86|
|[[:results:exp261|ABT-702 5μM R06 exp261]]|-1.79|
|[[:results:exp497|Lead acetate 2000μM R08 exp497]]|-1.73|
|[[:results:exp483|FTY720 3μM R08 exp483]]|-1.72|
|[[:results:exp99|NFN1 0.4μM R03 exp99]]|1.71|
|[[:results:exp112|S-DABN 8μM R03 exp112]]|1.75|
|[[:results:exp446|4-Nitroquinoline-1-oxide 0.5μM R08 exp446]]|1.77|
|[[:results:exp475|CyclicAMP 200μM R08 exp475]]|2.11|
|[[:results:exp466|Cannabidiol 20μM R08 exp466]]|2.12|
|[[:results:exp521|Salinomycin 3.5μM R08 exp521]]|2.61|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
No correlation found to any other genes in chemogenomics.
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 1/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|0/28|
|blood|0/28|
|bone|0/26|
|breast|0/33|
|central nervous system|0/56|
|cervix|0/4|
|colorectal|1/17|
|esophagus|0/13|
|fibroblast|0/1|
|gastric|0/16|
|kidney|0/21|
|liver|0/20|
|lung|0/75|
|lymphocyte|0/16|
|ovary|0/26|
|pancreas|0/24|
|peripheral nervous system|0/16|
|plasma cell|0/15|
|prostate|0/1|
|skin|0/24|
|soft tissue|0/9|
|thyroid|0/2|
|upper aerodigestive|0/22|
|urinary tract|0/29|
|uterus|0/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 13644
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 6.04 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='ROCK2 Expression in NALM6 Cells: 6.04'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>