RPL35A [The Human Chemical-Genetic Interaction Map Project]

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======= RPL35A =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: RPL35A
  * **<color #00a2e8>Official Name</color>**: ribosomal protein L35a
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=6165|6165]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/P18077|P18077]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=RPL35A&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20RPL35A|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/180468|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**:  Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L35AE family of ribosomal proteins. It is located in the cytoplasm. The rat protein has been shown to bind to both initiator and elongator tRNAs, and thus, it is located at the P site, or P and A sites, of the ribosome. Although this gene was originally mapped to chromosome 18, it has been established that it is located at 3q29-qter. Alternative splicing results in multiple transcript variants. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Oct 2015]. 
  * **<color #00a2e8>UniProt Summary</color>**:  Required for the proliferation and viability of hematopoietic cells. Plays a role in 60S ribosomal subunit formation. The protein was found to bind to both initiator and elongator tRNAs and consequently was assigned to the P site or P and A site. {ECO:0000269|PubMed:18535205}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|Ribosomal L35Ae|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|tRNA binding|
|cytosolic large ribosomal subunit|
|ribosomal large subunit biogenesis|
|cytoplasmic translation|
|SRP-dependent cotranslational protein targeting to membrane|
|cotranslational protein targeting to membrane|
|protein targeting to ER|
|establishment of protein localization to endoplasmic reticulum|
|viral transcription|
|nuclear-transcribed mRNA catabolic process, nonsense-mediated decay|
|viral gene expression|
|protein localization to endoplasmic reticulum|
|translational initiation|
|protein targeting to membrane|
|structural constituent of ribosome|
|nuclear-transcribed mRNA catabolic process|
|rRNA processing|
|mRNA catabolic process|
|rRNA metabolic process|
|RNA catabolic process|
|establishment of protein localization to membrane|
|ribosome biogenesis|
|protein targeting|
|nucleobase-containing compound catabolic process|
|translation|
|ncRNA processing|
|peptide biosynthetic process|
|establishment of protein localization to organelle|
|heterocycle catabolic process|
|cellular nitrogen compound catabolic process|
|aromatic compound catabolic process|
|ribonucleoprotein complex biogenesis|
|ncRNA metabolic process|
|organic cyclic compound catabolic process|
|protein localization to membrane|
|amide biosynthetic process|
|peptide metabolic process|
|mRNA metabolic process|
|viral process|
|protein localization to organelle|
|symbiotic process|
|cellular amide metabolic process|
|interspecies interaction between organisms|
|RNA processing|
|cellular macromolecule catabolic process|
|intracellular protein transport|
|macromolecule catabolic process|
|RNA binding|
|organonitrogen compound biosynthetic process|
|protein transport|
|intracellular transport|
|peptide transport|
|amide transport|
|cellular protein localization|
|cellular macromolecule localization|
|establishment of protein localization|
|cellular nitrogen compound biosynthetic process|
|RNA metabolic process|
|cellular macromolecule biosynthetic process|
|negative regulation of gene expression|
|macromolecule biosynthetic process|
|organic substance catabolic process|
|cellular catabolic process|
|establishment of localization in cell|
|nitrogen compound transport|
|membrane|
|gene expression|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp434|Vemurafenib 6.6μM R08 exp434]]|-2.03|
|[[:results:exp376|Losmapimod 1μM R07 exp376]]|1.7|
|[[:results:exp499|LY2090314 0.003μM R08 exp499]]|1.7|
|[[:results:exp12|Chloramphenicol 2μM R00 exp12]]|1.72|
|[[:results:exp5|Alpha-Amanitin 0.5μM R00 exp5]]|1.74|
|[[:results:exp464|Calcipotriol 5μM R08 exp464]]|1.74|
|[[:results:exp177|Apcin 25μM plus proTAME 2μM R04 exp177]]|1.76|
|[[:results:exp319|ABT-702 5μM plus Dimethyloxaloylglycine 11μM R07 exp319]]|1.8|
|[[:results:exp293|Myriocin 25μM R06 exp293]]|1.82|
|[[:results:exp162|BI-D1870 2μM R04 exp162]]|1.88|
|[[:results:exp40|2-Methoxyestradiol 0.2μM R01 exp40]]|1.92|
|[[:results:exp331|A-769662 20μM R07 exp331]]|1.92|
|[[:results:exp51|Nifuroxazide 1μM R01 exp51]]|1.94|
|[[:results:exp409|THZ531 0.11μM R07 exp409]]|1.94|
|[[:results:exp31|Rifampicin 1μM R00 exp31]]|1.96|
|[[:results:exp314|Dimethyloxaloylglycine 11μM R07 exp314]]|2.09|
|[[:results:exp437|Ethanol 0.003 R08 exp437]]|2.18|
|[[:results:exp444|THZ531 0.225μM R08 exp444]]|2.31|
|[[:results:exp287|HMS-I2 5μM R06 exp287]]|2.33|
|[[:results:exp412|THZ531 0.11 to 0.125 to 0.35μM on day4 then day6 R07 exp412]]|2.37|
|[[:results:exp449|Arsenic trioxide 60μM R08 exp449]]|2.52|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:m:mbtd1|MBTD1]]|0.424|
|[[:human genes:a:arcn1|ARCN1]]|0.42|
|[[:human genes:t:tbc1d3f|TBC1D3F]]|0.411|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 1/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|0/28|
|blood|0/28|
|bone|0/26|
|breast|0/33|
|central nervous system|1/56|
|cervix|0/4|
|colorectal|0/17|
|esophagus|0/13|
|fibroblast|0/1|
|gastric|0/16|
|kidney|0/21|
|liver|0/20|
|lung|0/75|
|lymphocyte|0/16|
|ovary|0/26|
|pancreas|0/24|
|peripheral nervous system|0/16|
|plasma cell|0/15|
|prostate|0/1|
|skin|0/24|
|soft tissue|0/9|
|thyroid|0/2|
|upper aerodigestive|0/22|
|urinary tract|0/29|
|uterus|0/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 89
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 8.11 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='RPL35A Expression in NALM6 Cells: 8.11'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>