RPL5 [The Human Chemical-Genetic Interaction Map Project]

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======= RPL5 =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: RPL5
  * **<color #00a2e8>Official Name</color>**: ribosomal protein L5
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=6125|6125]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/P46777|P46777]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=RPL5&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20RPL5|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/603634|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**:  Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of four RNA species and approximately 80 structurally distinct proteins. This gene encodes a member of the L18P family of ribosomal proteins and component of the 60S subunit. The encoded protein binds 5S rRNA to form a stable complex called the 5S ribonucleoprotein particle (RNP), which is necessary for the transport of nonribosome-associated cytoplasmic 5S rRNA to the nucleolus for assembly into ribosomes. The encoded protein may also function to inhibit tumorigenesis through the activation of downstream tumor suppressors and the downregulation of oncoprotein expression. Mutations in this gene have been identified in patients with Diamond-Blackfan Anemia (DBA). This gene is co-transcribed with the small nucleolar RNA gene U21, which is located in its fifth intron. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed throughout the genome. [provided by RefSeq, Mar 2017]. 
  * **<color #00a2e8>UniProt Summary</color>**:  Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl- transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel. As part of the 5S RNP/5S ribonucleoprotein particle it is an essential component of the LSU, required for its formation and the maturation of rRNAs (PubMed:12962325, PubMed:19061985, PubMed:24120868, PubMed:23636399). It also couples ribosome biogenesis to p53/TP53 activation. As part of the 5S RNP it accumulates in the nucleoplasm and inhibits MDM2, when ribosome biogenesis is perturbed, mediating the stabilization and the activation of TP53 (PubMed:24120868). {ECO:0000269|PubMed:12962325, ECO:0000269|PubMed:19061985, ECO:0000269|PubMed:23636399, ECO:0000269|PubMed:24120868}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|Ribosomal L18p|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|negative regulation of protein neddylation|
|regulation of protein neddylation|
|ubiquitin ligase inhibitor activity|
|negative regulation of ubiquitin protein ligase activity|
|5S rRNA binding|
|negative regulation of ubiquitin-protein transferase activity|
|regulation of ubiquitin protein ligase activity|
|mRNA 5-UTR binding|
|ribosomal large subunit assembly|
|negative regulation of ubiquitin-dependent protein catabolic process|
|regulation of ubiquitin-protein transferase activity|
|ribosome assembly|
|cytosolic large ribosomal subunit|
|negative regulation of proteolysis involved in cellular protein catabolic process|
|ribosomal large subunit biogenesis|
|negative regulation of protein ubiquitination|
|mRNA 3-UTR binding|
|negative regulation of protein modification by small protein conjugation or removal|
|negative regulation of cellular protein catabolic process|
|SRP-dependent cotranslational protein targeting to membrane|
|cotranslational protein targeting to membrane|
|protein targeting to ER|
|establishment of protein localization to endoplasmic reticulum|
|viral transcription|
|nuclear-transcribed mRNA catabolic process, nonsense-mediated decay|
|positive regulation of translation|
|viral gene expression|
|negative regulation of protein catabolic process|
|protein localization to endoplasmic reticulum|
|translational initiation|
|positive regulation of cellular amide metabolic process|
|regulation of ubiquitin-dependent protein catabolic process|
|ribonucleoprotein complex|
|protein targeting to membrane|
|structural constituent of ribosome|
|protein stabilization|
|regulation of signal transduction by p53 class mediator|
|nuclear-transcribed mRNA catabolic process|
|regulation of protein ubiquitination|
|rRNA processing|
|mRNA catabolic process|
|regulation of proteolysis involved in cellular protein catabolic process|
|rRNA metabolic process|
|regulation of protein modification by small protein conjugation or removal|
|ribonucleoprotein complex assembly|
|ribonucleoprotein complex subunit organization|
|RNA catabolic process|
|regulation of cellular protein catabolic process|
|negative regulation of cellular catabolic process|
|establishment of protein localization to membrane|
|negative regulation of transferase activity|
|regulation of protein stability|
|ubiquitin protein ligase binding|
|ribosome biogenesis|
|negative regulation of catabolic process|
|regulation of translation|
|negative regulation of proteolysis|
|protein targeting|
|nucleobase-containing compound catabolic process|
|translation|
|ncRNA processing|
|regulation of protein catabolic process|
|regulation of cellular amide metabolic process|
|peptide biosynthetic process|
|focal adhesion|
|establishment of protein localization to organelle|
|heterocycle catabolic process|
|cellular nitrogen compound catabolic process|
|aromatic compound catabolic process|
|ribonucleoprotein complex biogenesis|
|ncRNA metabolic process|
|organic cyclic compound catabolic process|
|protein localization to membrane|
|posttranscriptional regulation of gene expression|
|amide biosynthetic process|
|peptide metabolic process|
|negative regulation of protein modification process|
|protein-containing complex|
|mRNA metabolic process|
|viral process|
|regulation of proteolysis|
|protein localization to organelle|
|organelle assembly|
|symbiotic process|
|cellular amide metabolic process|
|negative regulation of catalytic activity|
|interspecies interaction between organisms|
|regulation of cellular catabolic process|
|cellular protein-containing complex assembly|
|nucleolus|
|RNA processing|
|cellular macromolecule catabolic process|
|regulation of transferase activity|
|intracellular protein transport|
|regulation of catabolic process|
|endoplasmic reticulum|
|negative regulation of cellular protein metabolic process|
|macromolecule catabolic process|
|negative regulation of protein metabolic process|
|negative regulation of molecular function|
|RNA binding|
|organonitrogen compound biosynthetic process|
|protein transport|
|intracellular transport|
|peptide transport|
|protein-containing complex assembly|
|amide transport|
|cellular protein localization|
|cellular macromolecule localization|
|positive regulation of cellular protein metabolic process|
|establishment of protein localization|
|cellular nitrogen compound biosynthetic process|
|RNA metabolic process|
|positive regulation of protein metabolic process|
|cellular macromolecule biosynthetic process|
|negative regulation of gene expression|
|macromolecule biosynthetic process|
|organic substance catabolic process|
|cellular catabolic process|
|regulation of intracellular signal transduction|
|establishment of localization in cell|
|nitrogen compound transport|
|regulation of protein modification process|
|protein-containing complex subunit organization|
|positive regulation of macromolecule biosynthetic process|
|positive regulation of cellular biosynthetic process|
|positive regulation of gene expression|
|membrane|
|gene expression|
|positive regulation of biosynthetic process|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp28|Pimelic-diphenylamide-106 5μM R00 exp28]]|-1.79|
|[[:results:exp240|Pyridostatin 4μM R05 exp240]]|1.7|
|[[:results:exp114|A-196 10μM R03 exp114]]|1.71|
|[[:results:exp42|BI-6727 0.001μM R01 exp42]]|1.71|
|[[:results:exp345|Cidofovir 10μM R07 exp345]]|1.73|
|[[:results:exp152|SGC2043 10μM R03 exp152]]|1.73|
|[[:results:exp4|Actinomycin-D 0.01μM R00 exp4]]|1.74|
|[[:results:exp442|Ibrutinib 10μM R08 exp442]]|1.74|
|[[:results:exp430|Rifampicin 30μM R08 exp430]]|1.8|
|[[:results:exp410|THZ531 0.11 to 0.125μM on day4 R07 exp410]]|1.81|
|[[:results:exp222|Betulinic acid 10 to 15μM on day4 R05 exp222]]|1.83|
|[[:results:exp125|GSK461364A 0.005μM R03 exp125]]|1.84|
|[[:results:exp209|Deguelin 0.15μM R05 exp209]]|1.91|
|[[:results:exp103|Taxol 0.004μM R03 exp103]]|1.94|
|[[:results:exp526|Sulforaphane 9μM R08 exp526 no dilution day6]]|1.95|
|[[:results:exp116|AICAR 240μM R03 exp116]]|2.25|
|[[:results:exp21|MLN-4924 0.2μM R00 exp21]]|2.28|
|[[:results:exp102|Nifuroxazide 5μM R03 exp102]]|2.28|
|[[:results:exp505|ML-792 0.2μM R08 exp505]]|2.31|
|[[:results:exp412|THZ531 0.11 to 0.125 to 0.35μM on day4 then day6 R07 exp412]]|2.32|
|[[:results:exp211|AICAR 240μM R05 exp211]]|2.33|
|[[:results:exp413|THZ531 0.11 to 0.175μM on day4 R07 exp413]]|2.33|
|[[:results:exp175|3-Bromopyruvate 7μM R04 exp175]]|2.41|
|[[:results:exp537|WNT3A 44ng/ml R08 exp537]]|2.57|
|[[:results:exp446|4-Nitroquinoline-1-oxide 0.5μM R08 exp446]]|2.92|
|[[:results:exp485|GSK626616 14μM R08 exp485]]|3.69|
|[[:results:exp444|THZ531 0.225μM R08 exp444]]|3.89|
|[[:results:exp3|Actinomycin-D 0.001μM R00 exp3]]|4|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:h:heatr3|HEATR3]]|0.596|
|[[:human genes:r:rpl11|RPL11]]|0.59|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 733/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|1/1|
|909776.0|1/1|
|bile duct|28/28|
|blood|26/28|
|bone|24/26|
|breast|33/33|
|central nervous system|56/56|
|cervix|4/4|
|colorectal|17/17|
|esophagus|13/13|
|fibroblast|1/1|
|gastric|16/16|
|kidney|20/21|
|liver|20/20|
|lung|75/75|
|lymphocyte|16/16|
|ovary|26/26|
|pancreas|24/24|
|peripheral nervous system|16/16|
|plasma cell|15/15|
|prostate|1/1|
|skin|24/24|
|soft tissue|9/9|
|thyroid|2/2|
|upper aerodigestive|22/22|
|urinary tract|29/29|
|uterus|5/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 788
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 9.51 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='RPL5 Expression in NALM6 Cells: 9.51'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>