RPS27A [The Human Chemical-Genetic Interaction Map Project]

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======= RPS27A =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: RPS27A
  * **<color #00a2e8>Official Name</color>**: ribosomal protein S27a
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=6233|6233]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/P62979|P62979]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=RPS27A&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20RPS27A|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/191343|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**:  Ubiquitin, a highly conserved protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome, is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin fused to an unrelated protein. This gene encodes a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein S27a at the C terminus. When expressed in yeast, the protein is post-translationally processed, generating free ubiquitin monomer and ribosomal protein S27a. Ribosomal protein S27a is a component of the 40S subunit of the ribosome and belongs to the S27AE family of ribosomal proteins. It contains C4-type zinc finger domains and is located in the cytoplasm. Pseudogenes derived from this gene are present in the genome. As with ribosomal protein S27a, ribosomal protein L40 is also synthesized as a fusion protein with ubiquitin; similarly, ribosomal protein S30 is synthesized as a fusion protein with the ubiquitin-like protein fubi. Multiple alternatively spliced transcript variants that encode the same proteins have been identified.[provided by RefSeq, Sep 2008]. 
  * **<color #00a2e8>UniProt Summary</color>**: N/A

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|Rad60-SLD|
|Ribosomal S27|
|ubiquitin|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|protein tag|
|small ribosomal subunit|
|error-prone translesion synthesis|
|error-free translesion synthesis|
|nucleotide-excision repair, DNA duplex unwinding|
|nucleotide-excision repair, DNA damage recognition|
|nucleotide-excision repair, DNA gap filling|
|global genome nucleotide-excision repair|
|nucleotide-binding oligomerization domain containing signaling pathway|
|nucleotide-binding domain, leucine rich repeat containing receptor signaling pathway|
|TRIF-dependent toll-like receptor signaling pathway|
|nucleotide-excision repair, preincision complex assembly|
|MyD88-dependent toll-like receptor signaling pathway|
|MyD88-independent toll-like receptor signaling pathway|
|cytoplasmic pattern recognition receptor signaling pathway|
|nucleotide-excision repair, DNA incision, 5-to lesion|
|virion assembly|
|DNA damage response, detection of DNA damage|
|nucleotide-excision repair, DNA incision|
|translesion synthesis|
|cytosolic small ribosomal subunit|
|DNA synthesis involved in DNA repair|
|interstrand cross-link repair|
|postreplication repair|
|intracellular transport of virus|
|host cell|
|transport of virus|
|establishment of protein localization to peroxisome|
|protein localization to peroxisome|
|endocytic vesicle membrane|
|protein targeting to peroxisome|
|I-kappaB kinase/NF-kappaB signaling|
|multi-organism localization|
|peroxisomal transport|
|multi-organism transport|
|transcription-coupled nucleotide-excision repair|
|negative regulation of transforming growth factor beta receptor signaling pathway|
|negative regulation of cellular response to transforming growth factor beta stimulus|
|regulation of transcription from RNA polymerase II promoter in response to hypoxia|
|peroxisome organization|
|JNK cascade|
|anaphase-promoting complex-dependent catabolic process|
|interleukin-1-mediated signaling pathway|
|SRP-dependent cotranslational protein targeting to membrane|
|toll-like receptor signaling pathway|
|cotranslational protein targeting to membrane|
|transforming growth factor beta receptor signaling pathway|
|stress-activated MAPK cascade|
|protein targeting to ER|
|DNA biosynthetic process|
|DNA duplex unwinding|
|nucleotide-excision repair|
|establishment of protein localization to endoplasmic reticulum|
|regulation of transforming growth factor beta receptor signaling pathway|
|viral transcription|
|negative regulation of transmembrane receptor protein serine/threonine kinase signaling pathway|
|regulation of cellular response to transforming growth factor beta stimulus|
|nuclear-transcribed mRNA catabolic process, nonsense-mediated decay|
|DNA geometric change|
|regulation of transcription from RNA polymerase II promoter in response to stress|
|regulation of DNA-templated transcription in response to stress|
|pattern recognition receptor signaling pathway|
|viral gene expression|
|protein localization to endoplasmic reticulum|
|vesicle|
|stress-activated protein kinase signaling cascade|
|translational initiation|
|negative regulation of cellular response to growth factor stimulus|
|positive regulation of NF-kappaB transcription factor activity|
|cellular response to transforming growth factor beta stimulus|
|activation of MAPK activity|
|protein targeting to membrane|
|response to transforming growth factor beta|
|mitochondrial outer membrane|
|intracellular receptor signaling pathway|
|structural constituent of ribosome|
|cellular response to interleukin-1|
|regulation of mRNA stability|
|regulation of RNA stability|
|cellular response to hypoxia|
|nuclear-transcribed mRNA catabolic process|
|cellular response to decreased oxygen levels|
|response to interleukin-1|
|transmembrane receptor protein serine/threonine kinase signaling pathway|
|regulation of mRNA catabolic process|
|viral life cycle|
|protein-DNA complex assembly|
|endosome membrane|
|cellular response to oxygen levels|
|mRNA catabolic process|
|endosomal transport|
|innate immune response-activating signal transduction|
|regulation of transmembrane receptor protein serine/threonine kinase signaling pathway|
|protein-DNA complex subunit organization|
|RNA catabolic process|
|activation of innate immune response|
|positive regulation of DNA-binding transcription factor activity|
|positive regulation of MAP kinase activity|
|establishment of protein localization to membrane|
|regulation of cellular response to growth factor stimulus|
|protein deubiquitination|
|ubiquitin protein ligase binding|
|DNA conformation change|
|protein modification by small protein removal|
|protein polyubiquitination|
|nucleic acid phosphodiester bond hydrolysis|
|proteasome-mediated ubiquitin-dependent protein catabolic process|
|regulation of mRNA metabolic process|
|activation of protein kinase activity|
|positive regulation of protein serine/threonine kinase activity|
|positive regulation of innate immune response|
|regulation of MAP kinase activity|
|response to hypoxia|
|proteasomal protein catabolic process|
|Wnt signaling pathway|
|protein targeting|
|response to decreased oxygen levels|
|cell-cell signaling by wnt|
|positive regulation of response to biotic stimulus|
|MAPK cascade|
|nucleobase-containing compound catabolic process|
|response to oxygen levels|
|translation|
|signal transduction by protein phosphorylation|
|peptide biosynthetic process|
|cell surface receptor signaling pathway involved in cell-cell signaling|
|regulation of DNA-binding transcription factor activity|
|establishment of protein localization to organelle|
|heterocycle catabolic process|
|cellular nitrogen compound catabolic process|
|aromatic compound catabolic process|
|regulation of innate immune response|
|positive regulation of defense response|
|organic cyclic compound catabolic process|
|protein localization to membrane|
|cellular response to growth factor stimulus|
|positive regulation of multi-organism process|
|DNA repair|
|regulation of protein serine/threonine kinase activity|
|regulation of response to biotic stimulus|
|posttranscriptional regulation of gene expression|
|amide biosynthetic process|
|ubiquitin-dependent protein catabolic process|
|peptide metabolic process|
|response to growth factor|
|modification-dependent protein catabolic process|
|positive regulation of protein kinase activity|
|positive regulation of MAPK cascade|
|modification-dependent macromolecule catabolic process|
|immune response-activating signal transduction|
|positive regulation of kinase activity|
|proteolysis involved in cellular protein catabolic process|
|immune response-regulating signaling pathway|
|positive regulation of response to external stimulus|
|cellular protein catabolic process|
|activation of immune response|
|positive regulation of apoptotic process|
|positive regulation of programmed cell death|
|positive regulation of transferase activity|
|cytokine-mediated signaling pathway|
|protein catabolic process|
|protein ubiquitination|
|detection of stimulus|
|mRNA metabolic process|
|positive regulation of cell death|
|viral process|
|enzyme linked receptor protein signaling pathway|
|protein localization to organelle|
|DNA metabolic process|
|regulation of MAPK cascade|
|regulation of defense response|
|protein modification by small protein conjugation|
|cellular response to DNA damage stimulus|
|regulation of multi-organism process|
|symbiotic process|
|cellular amide metabolic process|
|regulation of protein kinase activity|
|interspecies interaction between organisms|
|regulation of cellular catabolic process|
|cellular protein-containing complex assembly|
|membrane organization|
|nucleolus|
|negative regulation of transcription by RNA polymerase II|
|positive regulation of immune response|
|regulation of kinase activity|
|negative regulation of apoptotic process|
|cellular macromolecule catabolic process|
|negative regulation of programmed cell death|
|endoplasmic reticulum membrane|
|protein phosphorylation|
|regulation of transferase activity|
|protein modification by small protein conjugation or removal|
|intracellular protein transport|
|regulation of catabolic process|
|negative regulation of cell death|
|cellular response to cytokine stimulus|
|positive regulation of protein phosphorylation|
|positive regulation of intracellular signal transduction|
|macromolecule catabolic process|
|positive regulation of phosphorylation|
|organonitrogen compound catabolic process|
|chromosome organization|
|regulation of response to external stimulus|
|nucleobase-containing compound biosynthetic process|
|response to cytokine|
|cell-cell signaling|
|positive regulation of phosphate metabolic process|
|positive regulation of phosphorus metabolic process|
|regulation of immune response|
|positive regulation of immune system process|
|response to abiotic stimulus|
|heterocycle biosynthetic process|
|aromatic compound biosynthetic process|
|negative regulation of transcription, DNA-templated|
|positive regulation of transcription by RNA polymerase II|
|cellular response to endogenous stimulus|
|positive regulation of protein modification process|
|negative regulation of nucleic acid-templated transcription|
|negative regulation of RNA biosynthetic process|
|negative regulation of signal transduction|
|proteolysis|
|transmembrane transport|
|phosphorylation|
|organic cyclic compound biosynthetic process|
|negative regulation of RNA metabolic process|
|negative regulation of cell communication|
|negative regulation of signaling|
|negative regulation of cellular macromolecule biosynthetic process|
|RNA binding|
|organonitrogen compound biosynthetic process|
|positive regulation of catalytic activity|
|negative regulation of nucleobase-containing compound metabolic process|
|regulation of protein phosphorylation|
|negative regulation of macromolecule biosynthetic process|
|response to endogenous stimulus|
|regulation of response to stress|
|protein transport|
|negative regulation of cellular biosynthetic process|
|intracellular transport|
|peptide transport|
|positive regulation of transcription, DNA-templated|
|regulation of apoptotic process|
|negative regulation of biosynthetic process|
|protein-containing complex assembly|
|regulation of programmed cell death|
|amide transport|
|cellular protein localization|
|regulation of phosphorylation|
|cellular macromolecule localization|
|extracellular space|
|positive regulation of cellular protein metabolic process|
|establishment of protein localization|
|negative regulation of response to stimulus|
|cellular nitrogen compound biosynthetic process|
|positive regulation of nucleic acid-templated transcription|
|positive regulation of RNA biosynthetic process|
|positive regulation of signal transduction|
|regulation of immune system process|
|RNA metabolic process|
|regulation of cell death|
|intracellular signal transduction|
|cellular response to stress|
|positive regulation of protein metabolic process|
|cellular macromolecule biosynthetic process|
|negative regulation of gene expression|
|positive regulation of RNA metabolic process|
|macromolecule biosynthetic process|
|organic substance catabolic process|
|positive regulation of molecular function|
|regulation of phosphate metabolic process|
|regulation of phosphorus metabolic process|
|cellular catabolic process|
|positive regulation of cell communication|
|positive regulation of signaling|
|regulation of intracellular signal transduction|
|establishment of localization in cell|
|regulation of protein modification process|
|nitrogen compound transport|
|protein-containing complex subunit organization|
|positive regulation of nucleobase-containing compound metabolic process|
|positive regulation of macromolecule biosynthetic process|
|vesicle-mediated transport|
|positive regulation of cellular biosynthetic process|
|positive regulation of gene expression|
|membrane|
|gene expression|
|positive regulation of biosynthetic process|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp433|LJH685 50μM R08 exp433]]|-1.96|
|[[:results:exp478|Doxorubicin 0.02μM R08 exp478]]|-1.76|
|[[:results:exp477|DKK1 89ng/ml R08 exp477]]|1.75|
|[[:results:exp102|Nifuroxazide 5μM R03 exp102]]|1.8|
|[[:results:exp14|Cycloheximide 0.02μM R00 exp14]]|1.93|
|[[:results:exp533|TNF-alpha 44ng/ml R08 exp533]]|1.97|
|[[:results:exp125|GSK461364A 0.005μM R03 exp125]]|1.99|
|[[:results:exp95|BI-2536 0.0042μM R03 exp95]]|2.76|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:r:rps12|RPS12]]|0.498|
|[[:human genes:p:psmb1|PSMB1]]|0.466|
|[[:human genes:r:rps23|RPS23]]|0.463|
|[[:human genes:r:rps7|RPS7]]|0.453|
|[[:human genes:r:rps28|RPS28]]|0.446|
|[[:human genes:c:c19orf53|C19orf53]]|0.43|
|[[:human genes:r:rps3|RPS3]]|0.425|
|[[:human genes:r:rps16|RPS16]]|0.424|
|[[:human genes:r:rps17l|RPS17L]]|0.423|
|[[:human genes:r:rbm39|RBM39]]|0.422|
|[[:human genes:g:gga3|GGA3]]|0.421|
|[[:human genes:t:top1mt|TOP1MT]]|0.415|
|[[:human genes:t:terf2|TERF2]]|0.415|
|[[:human genes:b:bcl2l1|BCL2L1]]|0.412|
|[[:human genes:r:rps11|RPS11]]|0.405|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 727/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|1/1|
|909776.0|1/1|
|bile duct|28/28|
|blood|28/28|
|bone|25/26|
|breast|32/33|
|central nervous system|56/56|
|cervix|4/4|
|colorectal|17/17|
|esophagus|13/13|
|fibroblast|1/1|
|gastric|16/16|
|kidney|21/21|
|liver|20/20|
|lung|73/75|
|lymphocyte|15/16|
|ovary|26/26|
|pancreas|24/24|
|peripheral nervous system|15/16|
|plasma cell|15/15|
|prostate|1/1|
|skin|24/24|
|soft tissue|9/9|
|thyroid|2/2|
|upper aerodigestive|21/22|
|urinary tract|28/29|
|uterus|4/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 1053
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 9.2 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='RPS27A Expression in NALM6 Cells: 9.2'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>