RPS3 [The Human Chemical-Genetic Interaction Map Project]

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======= RPS3 =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: RPS3
  * **<color #00a2e8>Official Name</color>**: ribosomal protein S3
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=6188|6188]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/P23396|P23396]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=RPS3&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20RPS3|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/600454|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**: N/A
  * **<color #00a2e8>UniProt Summary</color>**:  Involved in translation as a component of the 40S small ribosomal subunit (PubMed:8706699). Has endonuclease activity and plays a role in repair of damaged DNA (PubMed:7775413). Cleaves phosphodiester bonds of DNAs containing altered bases with broad specificity and cleaves supercoiled DNA more efficiently than relaxed DNA (PubMed:15707971). Displays high binding affinity for 7,8-dihydro-8-oxoguanine (8-oxoG), a common DNA lesion caused by reactive oxygen species (ROS) (PubMed:14706345). Has also been shown to bind with similar affinity to intact and damaged DNA (PubMed:18610840). Stimulates the N-glycosylase activity of the base excision protein OGG1 (PubMed:15518571). Enhances the uracil excision activity of UNG1 (PubMed:18973764). Also stimulates the cleavage of the phosphodiester backbone by APEX1 (PubMed:18973764). When located in the mitochondrion, reduces cellular ROS levels and mitochondrial DNA damage (PubMed:23911537). Has also been shown to negatively regulate DNA repair in cells exposed to hydrogen peroxide (PubMed:17049931). Plays a role in regulating transcription as part of the NF-kappa-B p65-p50 complex where it binds to the RELA/p65 subunit, enhances binding of the complex to DNA and promotes transcription of target genes (PubMed:18045535). Represses its own translation by binding to its cognate mRNA (PubMed:20217897). Binds to and protects TP53/p53 from MDM2-mediated ubiquitination (PubMed:19656744). Involved in spindle formation and chromosome movement during mitosis by regulating microtubule polymerization (PubMed:23131551). Involved in induction of apoptosis through its role in activation of CASP8 (PubMed:14988002). Induces neuronal apoptosis by interacting with the E2F1 transcription factor and acting synergistically with it to up-regulate pro-apoptotic proteins BCL2L11/BIM and HRK/Dp5 (PubMed:20605787). Interacts with TRADD following exposure to UV radiation and induces apoptosis by caspase-dependent JNK activation (PubMed:22510408). {ECO:0000269|PubMed:14706345, ECO:0000269|PubMed:14988002, ECO:0000269|PubMed:15518571, ECO:0000269|PubMed:15707971, ECO:0000269|PubMed:17049931, ECO:0000269|PubMed:18045535, ECO:0000269|PubMed:18610840, ECO:0000269|PubMed:18973764, ECO:0000269|PubMed:19656744, ECO:0000269|PubMed:20217897, ECO:0000269|PubMed:20605787, ECO:0000269|PubMed:22510408, ECO:0000269|PubMed:23131551, ECO:0000269|PubMed:23911537, ECO:0000269|PubMed:7775413, ECO:0000269|PubMed:8706699}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|Ribosomal S3 C|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|oxidized pyrimidine DNA binding|
|response to TNF agonist|
|NF-kappaB complex|
|positive regulation of DNA N-glycosylase activity|
|regulation of base-excision repair|
|positive regulation of base-excision repair|
|positive regulation of cysteine-type endopeptidase activity involved in execution phase of apoptosis|
|regulation of DNA N-glycosylase activity|
|oxidized purine nucleobase lesion DNA N-glycosylase activity|
|positive regulation of endodeoxyribonuclease activity|
|regulation of cysteine-type endopeptidase activity involved in execution phase of apoptosis|
|positive regulation of deoxyribonuclease activity|
|supercoiled DNA binding|
|ubiquitin-like protein conjugating enzyme binding|
|positive regulation of intrinsic apoptotic signaling pathway in response to DNA damage|
|oxidized purine DNA binding|
|positive regulation of nuclease activity|
|iron-sulfur cluster binding|
|regulation of endodeoxyribonuclease activity|
|endodeoxyribonuclease activity|
|regulation of deoxyribonuclease activity|
|DNA N-glycosylase activity|
|class I DNA-(apurinic or apyrimidinic site) endonuclease activity|
|small ribosomal subunit rRNA binding|
|DNA-(apurinic or apyrimidinic site) endonuclease activity|
|positive regulation of T cell receptor signaling pathway|
|positive regulation of execution phase of apoptosis|
|positive regulation of activated T cell proliferation|
|positive regulation of antigen receptor-mediated signaling pathway|
|regulation of nuclease activity|
|protein kinase A binding|
|positive regulation of microtubule polymerization|
|positive regulation of interleukin-2 production|
|negative regulation of DNA repair|
|positive regulation of microtubule polymerization or depolymerization|
|regulation of activated T cell proliferation|
|DNA damage response, detection of DNA damage|
|regulation of intrinsic apoptotic signaling pathway in response to DNA damage|
|regulation of T cell receptor signaling pathway|
|Hsp90 protein binding|
|regulation of execution phase of apoptosis|
|Hsp70 protein binding|
|polysome|
|ribosome|
|cytosolic small ribosomal subunit|
|cellular response to nerve growth factor stimulus|
|regulation of microtubule polymerization|
|response to nerve growth factor|
|regulation of interleukin-2 production|
|tubulin binding|
|damaged DNA binding|
|positive regulation of intrinsic apoptotic signaling pathway|
|regulation of antigen receptor-mediated signaling pathway|
|positive regulation of DNA repair|
|cellular response to hydrogen peroxide|
|positive regulation of JUN kinase activity|
|negative regulation of protein ubiquitination|
|mitotic spindle|
|positive regulation of NIK/NF-kappaB signaling|
|negative regulation of response to DNA damage stimulus|
|regulation of microtubule polymerization or depolymerization|
|negative regulation of protein modification by small protein conjugation or removal|
|regulation of JUN kinase activity|
|kinase binding|
|positive regulation of T cell proliferation|
|ruffle membrane|
|SRP-dependent cotranslational protein targeting to membrane|
|positive regulation of response to DNA damage stimulus|
|spindle assembly|
|cotranslational protein targeting to membrane|
|regulation of NIK/NF-kappaB signaling|
|protein targeting to ER|
|establishment of protein localization to endoplasmic reticulum|
|viral transcription|
|cellular response to antibiotic|
|nuclear-transcribed mRNA catabolic process, nonsense-mediated decay|
|response to hydrogen peroxide|
|negative regulation of DNA metabolic process|
|regulation of DNA repair|
|positive regulation of protein polymerization|
|viral gene expression|
|positive regulation of lymphocyte proliferation|
|negative regulation of translation|
|positive regulation of mononuclear cell proliferation|
|cellular response to reactive oxygen species|
|positive regulation of cysteine-type endopeptidase activity involved in apoptotic process|
|protein localization to endoplasmic reticulum|
|positive regulation of leukocyte proliferation|
|positive regulation of JNK cascade|
|translational initiation|
|negative regulation of cellular amide metabolic process|
|positive regulation of cysteine-type endopeptidase activity|
|positive regulation of NF-kappaB transcription factor activity|
|ribonucleoprotein complex|
|spindle organization|
|regulation of T cell proliferation|
|protein targeting to membrane|
|regulation of intrinsic apoptotic signaling pathway|
|mRNA binding|
|positive regulation of stress-activated MAPK cascade|
|structural constituent of ribosome|
|positive regulation of stress-activated protein kinase signaling cascade|
|positive regulation of endopeptidase activity|
|positive regulation of apoptotic signaling pathway|
|regulation of JNK cascade|
|regulation of microtubule cytoskeleton organization|
|positive regulation of peptidase activity|
|positive regulation of DNA metabolic process|
|response to reactive oxygen species|
|nuclear-transcribed mRNA catabolic process|
|positive regulation of supramolecular fiber organization|
|regulation of protein ubiquitination|
|positive regulation of T cell activation|
|regulation of lymphocyte proliferation|
|regulation of mononuclear cell proliferation|
|cellular response to toxic substance|
|regulation of cysteine-type endopeptidase activity involved in apoptotic process|
|positive regulation of leukocyte cell-cell adhesion|
|mRNA catabolic process|
|regulation of response to DNA damage stimulus|
|positive regulation of cytoskeleton organization|
|regulation of leukocyte proliferation|
|regulation of microtubule-based process|
|regulation of protein polymerization|
|regulation of protein modification by small protein conjugation or removal|
|regulation of stress-activated MAPK cascade|
|regulation of stress-activated protein kinase signaling cascade|
|microtubule binding|
|cellular response to tumor necrosis factor|
|cellular response to oxidative stress|
|regulation of cysteine-type endopeptidase activity|
|RNA catabolic process|
|positive regulation of protein complex assembly|
|postsynaptic density|
|positive regulation of cell-cell adhesion|
|positive regulation of MAP kinase activity|
|positive regulation of DNA-binding transcription factor activity|
|response to tumor necrosis factor|
|establishment of protein localization to membrane|
|protein-containing complex binding|
|chromosome segregation|
|regulation of leukocyte cell-cell adhesion|
|response to antibiotic|
|RNA polymerase II regulatory region sequence-specific DNA binding|
|regulation of T cell activation|
|transcription factor binding|
|positive regulation of protein serine/threonine kinase activity|
|regulation of MAP kinase activity|
|enzyme binding|
|regulation of supramolecular fiber organization|
|regulation of translation|
|regulation of DNA metabolic process|
|protein targeting|
|positive regulation of proteolysis|
|positive regulation of lymphocyte activation|
|mitochondrial matrix|
|nucleobase-containing compound catabolic process|
|translation|
|response to oxidative stress|
|mitochondrial inner membrane|
|regulation of apoptotic signaling pathway|
|regulation of cell-cell adhesion|
|positive regulation of cell adhesion|
|regulation of cellular amide metabolic process|
|positive regulation of leukocyte activation|
|cellular response to drug|
|peptide biosynthetic process|
|focal adhesion|
|regulation of endopeptidase activity|
|positive regulation of cell activation|
|dendrite|
|regulation of DNA-binding transcription factor activity|
|establishment of protein localization to organelle|
|heterocycle catabolic process|
|cellular nitrogen compound catabolic process|
|aromatic compound catabolic process|
|regulation of peptidase activity|
|positive regulation of cytokine production|
|regulation of protein complex assembly|
|protein kinase binding|
|microtubule cytoskeleton organization|
|protein localization to membrane|
|organic cyclic compound catabolic process|
|cell division|
|cellular response to growth factor stimulus|
|response to toxic substance|
|DNA repair|
|regulation of lymphocyte activation|
|regulation of protein serine/threonine kinase activity|
|positive regulation of cellular component biogenesis|
|posttranscriptional regulation of gene expression|
|amide biosynthetic process|
|peptide metabolic process|
|response to growth factor|
|positive regulation of protein kinase activity|
|response to inorganic substance|
|regulation of cytoskeleton organization|
|positive regulation of MAPK cascade|
|positive regulation of kinase activity|
|negative regulation of protein modification process|
|regulation of leukocyte activation|
|positive regulation of organelle organization|
|regulation of cell activation|
|positive regulation of apoptotic process|
|positive regulation of programmed cell death|
|positive regulation of transferase activity|
|microtubule-based process|
|regulation of cell adhesion|
|detection of stimulus|
|regulation of cytokine production|
|mRNA metabolic process|
|positive regulation of cell death|
|viral process|
|regulation of proteolysis|
|regulation of cellular response to stress|
|protein localization to organelle|
|DNA metabolic process|
|regulation of MAPK cascade|
|organelle assembly|
|positive regulation of hydrolase activity|
|cellular response to DNA damage stimulus|
|symbiotic process|
|cellular amide metabolic process|
|regulation of protein kinase activity|
|interspecies interaction between organisms|
|nucleolus|
|positive regulation of immune response|
|regulation of kinase activity|
|cellular macromolecule catabolic process|
|positive regulation of cell population proliferation|
|apoptotic process|
|regulation of cellular component biogenesis|
|regulation of transferase activity|
|intracellular protein transport|
|cell cycle process|
|cellular response to cytokine stimulus|
|endoplasmic reticulum|
|positive regulation of protein phosphorylation|
|response to drug|
|positive regulation of intracellular signal transduction|
|negative regulation of cellular protein metabolic process|
|macromolecule catabolic process|
|programmed cell death|
|cellular response to oxygen-containing compound|
|positive regulation of phosphorylation|
|cell death|
|response to cytokine|
|negative regulation of protein metabolic process|
|cytoskeleton organization|
|positive regulation of phosphorus metabolic process|
|positive regulation of phosphate metabolic process|
|positive regulation of immune system process|
|regulation of immune response|
|positive regulation of cellular component organization|
|cellular response to endogenous stimulus|
|positive regulation of protein modification process|
|regulation of hydrolase activity|
|regulation of organelle organization|
|cell cycle|
|negative regulation of cellular macromolecule biosynthetic process|
|RNA binding|
|organonitrogen compound biosynthetic process|
|positive regulation of catalytic activity|
|negative regulation of nucleobase-containing compound metabolic process|
|regulation of protein phosphorylation|
|DNA binding|
|negative regulation of macromolecule biosynthetic process|
|response to endogenous stimulus|
|regulation of response to stress|
|protein transport|
|negative regulation of cellular biosynthetic process|
|intracellular transport|
|peptide transport|
|regulation of apoptotic process|
|negative regulation of biosynthetic process|
|response to oxygen-containing compound|
|regulation of programmed cell death|
|amide transport|
|cellular protein localization|
|regulation of phosphorylation|
|cellular macromolecule localization|
|positive regulation of cellular protein metabolic process|
|establishment of protein localization|
|regulation of cell population proliferation|
|negative regulation of response to stimulus|
|cellular nitrogen compound biosynthetic process|
|regulation of immune system process|
|positive regulation of signal transduction|
|RNA metabolic process|
|regulation of cell death|
|cellular response to stress|
|positive regulation of protein metabolic process|
|cellular macromolecule biosynthetic process|
|negative regulation of gene expression|
|positive regulation of multicellular organismal process|
|macromolecule biosynthetic process|
|organic substance catabolic process|
|positive regulation of molecular function|
|regulation of phosphate metabolic process|
|regulation of phosphorus metabolic process|
|cellular catabolic process|
|positive regulation of cell communication|
|positive regulation of signaling|
|regulation of intracellular signal transduction|
|establishment of localization in cell|
|nitrogen compound transport|
|regulation of protein modification process|
|positive regulation of nucleobase-containing compound metabolic process|
|positive regulation of gene expression|
|membrane|
|gene expression|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp106|UM131593 0.2μM R03 exp106]]|-2.14|
|[[:results:exp521|Salinomycin 3.5μM R08 exp521]]|-1.77|
|[[:results:exp5|Alpha-Amanitin 0.5μM R00 exp5]]|1.73|
|[[:results:exp429|Rapamycin 0.001μM R08 exp429]]|1.73|
|[[:results:exp336|Asunaprenir 3μM R07 exp336]]|1.82|
|[[:results:exp365|I-BRD9 4μM R07 exp365]]|1.83|
|[[:results:exp66|BI-D1870 3.15μM R02 exp66]]|1.93|
|[[:results:exp239|PFI-2 4μM R05 exp239]]|1.96|
|[[:results:exp263|Aphidicolin 0.04μM R06 exp263]]|2|
|[[:results:exp51|Nifuroxazide 1μM R01 exp51]]|2|
|[[:results:exp89|Vemurafenib 6.6μM R02 exp89]]|2.02|
|[[:results:exp449|Arsenic trioxide 60μM R08 exp449]]|2.14|
|[[:results:exp423|Zebularine 20μM R07 exp423]]|2.2|
|[[:results:exp73|LJH685 5μM R02 exp73]]|2.28|
|[[:results:exp391|Pomalidomide 20μM R07 exp391]]|2.57|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:r:rps3a|RPS3A]]|0.545|
|[[:human genes:r:rps12|RPS12]]|0.499|
|[[:human genes:c:cdc73|CDC73]]|0.48|
|[[:human genes:r:rps16|RPS16]]|0.477|
|[[:human genes:d:dtl|DTL]]|0.448|
|[[:human genes:p:paf1|PAF1]]|0.441|
|[[:human genes:r:rps28|RPS28]]|0.44|
|[[:human genes:a:adrm1|ADRM1]]|0.44|
|[[:human genes:c:cdca3|CDCA3]]|0.439|
|[[:human genes:p:psma6|PSMA6]]|0.437|
|[[:human genes:z:znf830|ZNF830]]|0.436|
|[[:human genes:z:zfand5|ZFAND5]]|0.435|
|[[:human genes:p:psmb2|PSMB2]]|0.434|
|[[:human genes:b:bcl2l1|BCL2L1]]|0.433|
|[[:human genes:w:wdr43|WDR43]]|0.432|
|[[:human genes:z:znf236|ZNF236]]|0.431|
|[[:human genes:p:psmb4|PSMB4]]|0.428|
|[[:human genes:r:rps27a|RPS27A]]|0.425|
|[[:human genes:s:smg7|SMG7]]|0.424|
|[[:human genes:v:vasn|VASN]]|0.424|
|[[:human genes:r:rpl35|RPL35]]|0.424|
|[[:human genes:r:ranbp2|RANBP2]]|0.422|
|[[:human genes:t:tceb3c|TCEB3C]]|0.421|
|[[:human genes:r:rps9|RPS9]]|0.42|
|[[:human genes:n:nol10|NOL10]]|0.419|
|[[:human genes:c:chaf1a|CHAF1A]]|0.419|
|[[:human genes:n:nup54|NUP54]]|0.416|
|[[:human genes:r:rps2|RPS2]]|0.414|
|[[:human genes:p:pola2|POLA2]]|0.414|
|[[:human genes:t:tceb3cl2|TCEB3CL2]]|0.412|
|[[:human genes:c:cdk8|CDK8]]|0.412|
|[[:human genes:p:psmd2|PSMD2]]|0.411|
|[[:human genes:o:orc6|ORC6]]|0.409|
|[[:human genes:p:pdcd11|PDCD11]]|0.408|
|[[:human genes:p:psmb1|PSMB1]]|0.407|
|[[:human genes:p:pole|POLE]]|0.406|
|[[:human genes:k:kctd17|KCTD17]]|0.406|
|[[:human genes:s:sf3a3|SF3A3]]|0.405|
|[[:human genes:m:mdm2|MDM2]]|0.405|
|[[:human genes:g:golga6l1|GOLGA6L1]]|0.404|
|[[:human genes:a:atp13a1|ATP13A1]]|0.404|
|[[:human genes:l:lrr1|LRR1]]|0.403|
|[[:human genes:r:rps14|RPS14]]|0.401|
|[[:human genes:p:prim1|PRIM1]]|0.4|
|[[:human genes:o:orc1|ORC1]]|0.4|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 713/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|1/1|
|909776.0|1/1|
|bile duct|28/28|
|blood|27/28|
|bone|26/26|
|breast|30/33|
|central nervous system|56/56|
|cervix|4/4|
|colorectal|17/17|
|esophagus|13/13|
|fibroblast|1/1|
|gastric|14/16|
|kidney|21/21|
|liver|18/20|
|lung|73/75|
|lymphocyte|16/16|
|ovary|25/26|
|pancreas|24/24|
|peripheral nervous system|16/16|
|plasma cell|15/15|
|prostate|1/1|
|skin|23/24|
|soft tissue|9/9|
|thyroid|2/2|
|upper aerodigestive|19/22|
|urinary tract|26/29|
|uterus|4/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 343
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 9.83 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='RPS3 Expression in NALM6 Cells: 9.83'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>