SPOP [The Human Chemical-Genetic Interaction Map Project]

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======= SPOP =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: SPOP
  * **<color #00a2e8>Official Name</color>**: speckle type BTB/POZ protein
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=8405|8405]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/O43791|O43791]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=SPOP&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20SPOP|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/602650|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**:  This gene encodes a protein that may modulate the transcriptional repression activities of death-associated protein 6 (DAXX), which interacts with histone deacetylase, core histones, and other histone-associated proteins. In mouse, the encoded protein binds to the putative leucine zipper domain of macroH2A1.2, a variant H2A histone that is enriched on inactivated X chromosomes. The BTB/POZ domain of this protein has been shown in other proteins to mediate transcriptional repression and to interact with components of histone deacetylase co-repressor complexes. Alternative splicing of this gene results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]. 
  * **<color #00a2e8>UniProt Summary</color>**:  Component of a cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex that mediates the ubiquitination of target proteins, leading most often to their proteasomal degradation. In complex with CUL3, involved in ubiquitination and proteasomal degradation of BRMS1, DAXX, PDX1/IPF1, GLI2 and GLI3. In complex with CUL3, involved in ubiquitination of H2AFY and BMI1; this does not lead to their proteasomal degradation. Inhibits transcriptional activation of PDX1/IPF1 targets, such as insulin, by promoting PDX1/IPF1 degradation. The cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex containing homodimeric SPOP has higher ubiquitin ligase activity than the complex that contains the heterodimer formed by SPOP and SPOPL. {ECO:0000269|PubMed:14528312, ECO:0000269|PubMed:15897469, ECO:0000269|PubMed:16524876, ECO:0000269|PubMed:19818708, ECO:0000269|PubMed:22085717, ECO:0000269|PubMed:22632832}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|MATH|
|BTB|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|Cul3-RING ubiquitin ligase complex|
|ubiquitin protein ligase binding|
|proteasome-mediated ubiquitin-dependent protein catabolic process|
|proteasomal protein catabolic process|
|nuclear speck|
|ubiquitin-dependent protein catabolic process|
|modification-dependent protein catabolic process|
|modification-dependent macromolecule catabolic process|
|proteolysis involved in cellular protein catabolic process|
|cellular protein catabolic process|
|protein catabolic process|
|protein ubiquitination|
|regulation of proteolysis|
|protein modification by small protein conjugation|
|cellular macromolecule catabolic process|
|protein modification by small protein conjugation or removal|
|macromolecule catabolic process|
|organonitrogen compound catabolic process|
|proteolysis|
|organic substance catabolic process|
|cellular catabolic process|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp122|Golgicide-A 4μM R03 exp122]]|-1.99|
|[[:results:exp312|2-Methoxyestradiol 0.55 to 1μM on day4 R07 exp312]]|1.73|
|[[:results:exp19|Etoposide 1μM R00 exp19]]|1.74|
|[[:results:exp294|Nutlin-3A 1.6μM R06 exp294]]|1.74|
|[[:results:exp333|All-trans-Retinoic-Acid 8μM R07 exp333]]|1.8|
|[[:results:exp311|2-Methoxyestradiol 0.55 to 0.75μM  on day4 R07 exp311]]|1.82|
|[[:results:exp175|3-Bromopyruvate 7μM R04 exp175]]|1.83|
|[[:results:exp359|FK-506 30μM R07 exp359]]|1.91|
|[[:results:exp264|Arsenate 40μM R06 exp264]]|1.91|
|[[:results:exp242|Radicicol 0.16μM R05 exp242]]|1.94|
|[[:results:exp162|BI-D1870 2μM R04 exp162]]|1.99|
|[[:results:exp259|6-Thio-2-deoxyguanosine 2μM R06 exp259]]|2|
|[[:results:exp513|ONC212 0.15μM R08 exp513]]|2|
|[[:results:exp497|Lead acetate 2000μM R08 exp497]]|2.01|
|[[:results:exp531|THZ1 0.06μM R08 exp531]]|2.03|
|[[:results:exp28|Pimelic-diphenylamide-106 5μM R00 exp28]]|2.11|
|[[:results:exp106|UM131593 0.2μM R03 exp106]]|2.14|
|[[:results:exp68|Clomiphene 4.4μM R02 exp68]]|2.15|
|[[:results:exp211|AICAR 240μM R05 exp211]]|2.17|
|[[:results:exp454|Bafilomycin-A1 0.009μM R08 exp454]]|2.22|
|[[:results:exp512|Olaparib 4μM R08 exp512]]|2.24|
|[[:results:exp85|UM0129480 7μM R02 exp85]]|2.24|
|[[:results:exp334|All-trans-Retinoic-Acid 40μM R07 exp334]]|2.32|
|[[:results:exp34|Rotenone 20μM R00 exp34]]|2.44|
|[[:results:exp407|Thapsigargin 0.005μM R07 exp407]]|2.44|
|[[:results:exp354|Diepoxybutane 3μM R07 exp354]]|2.48|
|[[:results:exp81|Selumetinib 20μM R02 exp81]]|2.53|
|[[:results:exp348|Cyclosporin-A 3μM R07 exp348]]|2.54|
|[[:results:exp460|BML-284 0.09μM R08 exp460]]|2.67|
|[[:results:exp66|BI-D1870 3.15μM R02 exp66]]|2.73|
|[[:results:exp217|Mdivi-1 15μM R05 exp217]]|2.73|
|[[:results:exp502|Milciclib 2μM R08 exp502]]|3|
|[[:results:exp2|5-Fluorouracil 20μM R00 exp2]]|3.06|
|[[:results:exp120|Dimethyl-Sulfoxide 1pc R03 exp120]]|3.12|
|[[:results:exp459|Bleomycin 5μM R08 exp459]]|3.21|
|[[:results:exp36|TRAIL 50ng/ml R00 exp36]]|4.64|
|[[:results:exp35|TRAIL 5ng/ml R00 exp35]]|4.65|
|[[:results:exp365|I-BRD9 4μM R07 exp365]]|4.69|
|[[:results:exp3|Actinomycin-D 0.001μM R00 exp3]]|5|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:f:fbxo11|FBXO11]]|0.515|
|[[:human genes:t:tp53|TP53]]|0.446|
|[[:human genes:s:ss18|SS18]]|0.446|
|[[:human genes:c:chd7|CHD7]]|0.441|
|[[:human genes:s:snrpa|SNRPA]]|0.436|
|[[:human genes:h:hars|HARS]]|0.427|
|[[:human genes:f:fnip1|FNIP1]]|0.42|
|[[:human genes:t:thoc6|THOC6]]|0.415|
|[[:human genes:b:btaf1|BTAF1]]|0.411|
|[[:human genes:c:cul3|CUL3]]|0.407|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 0/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|0/28|
|blood|0/28|
|bone|0/26|
|breast|0/33|
|central nervous system|0/56|
|cervix|0/4|
|colorectal|0/17|
|esophagus|0/13|
|fibroblast|0/1|
|gastric|0/16|
|kidney|0/21|
|liver|0/20|
|lung|0/75|
|lymphocyte|0/16|
|ovary|0/26|
|pancreas|0/24|
|peripheral nervous system|0/16|
|plasma cell|0/15|
|prostate|0/1|
|skin|0/24|
|soft tissue|0/9|
|thyroid|0/2|
|upper aerodigestive|0/22|
|urinary tract|0/29|
|uterus|0/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 2268
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 5.99 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='SPOP Expression in NALM6 Cells: 5.99'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>