TOP3A [The Human Chemical-Genetic Interaction Map Project]

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======= TOP3A =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: TOP3A
  * **<color #00a2e8>Official Name</color>**: DNA topoisomerase III alpha
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=7156|7156]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/Q13472|Q13472]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=TOP3A&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20TOP3A|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/601243|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**: N/A
  * **<color #00a2e8>UniProt Summary</color>**:  Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. Essential component of the RMI complex, a complex that plays an important role in the processing of homologous recombination intermediates to limit DNA crossover formation in cells. Has DNA decatenation activity. {ECO:0000269|PubMed:20445207, ECO:0000269|PubMed:8622991}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|Toprim|
|Topoisom bac|
|zf-C4 Topoisom|
|zf-GRF|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|DNA topoisomerase activity|
|DNA topoisomerase type I activity|
|DNA topological change|
|mitochondrial DNA metabolic process|
|mitochondrial genome maintenance|
|chromosome separation|
|PML body|
|single-stranded DNA binding|
|chromosome|
|regulation of signal transduction by p53 class mediator|
|DNA replication|
|meiotic cell cycle|
|chromosome segregation|
|DNA conformation change|
|mitochondrial matrix|
|mitochondrion organization|
|DNA metabolic process|
|zinc ion binding|
|cell cycle process|
|chromosome organization|
|cell cycle|
|reproductive process|
|reproduction|
|DNA binding|
|cellular macromolecule biosynthetic process|
|macromolecule biosynthetic process|
|regulation of intracellular signal transduction|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp422|Wiskostatin 3μM R07 exp422]]|-1.98|
|[[:results:exp183|IU1-C 25μM R04 exp183]]|-1.97|
|[[:results:exp49|NFN1 0.1μM R01 exp49]]|-1.89|
|[[:results:exp358|FK-506 5μM R07 exp358]]|-1.71|
|[[:results:exp42|BI-6727 0.001μM R01 exp42]]|-1.7|
|[[:results:exp294|Nutlin-3A 1.6μM R06 exp294]]|3.85|
|[[:results:exp354|Diepoxybutane 3μM R07 exp354]]|5.37|
|[[:results:exp299|Talazoparib 0.006μM R06 exp299]]|6.23|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:r:rmi2|RMI2]]|0.653|
|[[:human genes:r:rmi1|RMI1]]|0.609|
|[[:human genes:b:blm|BLM]]|0.547|
|[[:human genes:p:pml|PML]]|0.54|
|[[:human genes:p:prkrir|PRKRIR]]|0.487|
|[[:human genes:t:triap1|TRIAP1]]|0.437|
|[[:human genes:h:hars|HARS]]|0.413|
|[[:human genes:p:ppa2|PPA2]]|0.402|
|[[:human genes:a:alg1|ALG1]]|0.4|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 620/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|1/1|
|bile duct|24/28|
|blood|23/28|
|bone|20/26|
|breast|30/33|
|central nervous system|51/56|
|cervix|3/4|
|colorectal|16/17|
|esophagus|11/13|
|fibroblast|1/1|
|gastric|13/16|
|kidney|18/21|
|liver|18/20|
|lung|66/75|
|lymphocyte|11/16|
|ovary|22/26|
|pancreas|20/24|
|peripheral nervous system|14/16|
|plasma cell|12/15|
|prostate|1/1|
|skin|20/24|
|soft tissue|8/9|
|thyroid|2/2|
|upper aerodigestive|20/22|
|urinary tract|25/29|
|uterus|5/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 1727
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 6.83 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='TOP3A Expression in NALM6 Cells: 6.83'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>