U2AF2 [The Human Chemical-Genetic Interaction Map Project]

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======= U2AF2 =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: U2AF2
  * **<color #00a2e8>Official Name</color>**: U2 small nuclear RNA auxiliary factor 2
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=11338|11338]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/P26368|P26368]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=U2AF2&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20U2AF2|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/191318|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**:  U2 auxiliary factor (U2AF), comprised of a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the U2AF large subunit which contains a sequence-specific RNA-binding region with 3 RNA recognition motifs and an Arg/Ser-rich domain necessary for splicing. The large subunit binds to the polypyrimidine tract of introns early during spliceosome assembly. Multiple transcript variants have been detected for this gene, but the full-length natures of only two have been determined to date. [provided by RefSeq, Jul 2008]. 
  * **<color #00a2e8>UniProt Summary</color>**:  Necessary for the splicing of pre-mRNA. By recruiting PRPF19 and the PRP19C/Prp19 complex/NTC/Nineteen complex to the RNA polymerase II C-terminal domain (CTD), and thereby pre-mRNA, may couple transcription to splicing (PubMed:21536736). Induces cardiac troponin-T (TNNT2) pre-mRNA exon inclusion in muscle. Regulates the TNNT2 exon 5 inclusion through competition with MBNL1. Binds preferentially to a single-stranded structure within the polypyrimidine tract of TNNT2 intron 4 during spliceosome assembly. Required for the export of mRNA out of the nucleus, even if the mRNA is encoded by an intron-less gene. Represses the splicing of MAPT/Tau exon 10. {ECO:0000269|PubMed:15009664, ECO:0000269|PubMed:19470458, ECO:0000269|PubMed:19574390, ECO:0000269|PubMed:21536736}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|RRM 1|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|poly-pyrimidine tract binding|
|commitment complex|
|U2AF|
|pre-mRNA 3-splice site binding|
|Prp19 complex|
|C2H2 zinc finger domain binding|
|U2-type prespliceosome|
|negative regulation of mRNA splicing, via spliceosome|
|negative regulation of RNA splicing|
|negative regulation of mRNA processing|
|positive regulation of RNA splicing|
|negative regulation of protein ubiquitination|
|negative regulation of mRNA metabolic process|
|mRNA 3-end processing|
|negative regulation of protein modification by small protein conjugation or removal|
|spliceosomal complex|
|regulation of mRNA splicing, via spliceosome|
|mRNA-containing ribonucleoprotein complex export from nucleus|
|mRNA export from nucleus|
|ribonucleoprotein complex export from nucleus|
|ribonucleoprotein complex localization|
|RNA export from nucleus|
|RNA 3-end processing|
|regulation of RNA splicing|
|regulation of mRNA processing|
|protein export from nucleus|
|mRNA transport|
|nuclear export|
|RNA transport|
|nucleic acid transport|
|establishment of RNA localization|
|regulation of protein ubiquitination|
|RNA localization|
|regulation of protein modification by small protein conjugation or removal|
|nucleobase-containing compound transport|
|protein-containing complex localization|
|nucleocytoplasmic transport|
|nuclear transport|
|mRNA splicing, via spliceosome|
|RNA splicing, via transesterification reactions with bulged adenosine as nucleophile|
|RNA splicing, via transesterification reactions|
|regulation of mRNA metabolic process|
|enzyme binding|
|RNA splicing|
|nuclear speck|
|mRNA processing|
|negative regulation of protein modification process|
|mRNA metabolic process|
|RNA processing|
|intracellular protein transport|
|negative regulation of cellular protein metabolic process|
|negative regulation of protein metabolic process|
|negative regulation of RNA metabolic process|
|RNA binding|
|negative regulation of nucleobase-containing compound metabolic process|
|protein transport|
|intracellular transport|
|peptide transport|
|amide transport|
|cellular protein localization|
|cellular macromolecule localization|
|establishment of protein localization|
|RNA metabolic process|
|negative regulation of gene expression|
|positive regulation of RNA metabolic process|
|establishment of localization in cell|
|regulation of protein modification process|
|nitrogen compound transport|
|positive regulation of nucleobase-containing compound metabolic process|
|positive regulation of gene expression|
|gene expression|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp502|Milciclib 2μM R08 exp502]]|-2.15|
|[[:results:exp82|Torin1 0.08μM R02 exp82]]|-2.02|
|[[:results:exp350|Deferoxamine 11μM R07 exp350]]|-1.99|
|[[:results:exp70|INK128 0.2μM R02 exp70]]|-1.97|
|[[:results:exp459|Bleomycin 5μM R08 exp459]]|-1.73|
|[[:results:exp463|Caffeine 2600μM R08 exp463]]|-1.71|
|[[:results:exp478|Doxorubicin 0.02μM R08 exp478]]|1.73|
|[[:results:exp335|Aminopterin 0.005μM R07 exp335]]|1.88|
|[[:results:exp102|Nifuroxazide 5μM R03 exp102]]|1.92|
|[[:results:exp497|Lead acetate 2000μM R08 exp497]]|1.93|
|[[:results:exp480|ETC-159 50μM R08 exp480]]|1.99|
|[[:results:exp42|BI-6727 0.001μM R01 exp42]]|2.17|
|[[:results:exp51|Nifuroxazide 1μM R01 exp51]]|2.19|
|[[:results:exp224|CB-839 10μM R05 exp224]]|2.28|
|[[:results:exp47|Lapatinib 5μM R01 exp47]]|2.28|
|[[:results:exp299|Talazoparib 0.006μM R06 exp299]]|3.11|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:r:rrm1|RRM1]]|0.527|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 713/726
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|1/1|
|909776.0|1/1|
|bile duct|28/28|
|blood|28/28|
|bone|25/25|
|breast|31/33|
|central nervous system|56/56|
|cervix|4/4|
|colorectal|17/17|
|esophagus|13/13|
|fibroblast|1/1|
|gastric|15/15|
|kidney|21/21|
|liver|19/20|
|lung|74/75|
|lymphocyte|14/14|
|ovary|25/26|
|pancreas|24/24|
|peripheral nervous system|16/16|
|plasma cell|15/15|
|prostate|1/1|
|skin|21/24|
|soft tissue|7/7|
|thyroid|2/2|
|upper aerodigestive|22/22|
|urinary tract|29/29|
|uterus|5/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 1169
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 8.35 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='U2AF2 Expression in NALM6 Cells: 8.35'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>