WASH1 [The Human Chemical-Genetic Interaction Map Project]

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======= WASH1 =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: WASHC1
  * **<color #00a2e8>Official Name</color>**: WASH complex subunit 1
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=100287171|100287171]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/A8K0Z3|A8K0Z3]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=WASH1&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20WASH1|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/613632|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**: N/A
  * **<color #00a2e8>UniProt Summary</color>**:  Acts as a nucleation-promoting factor (NPF) at the surface of endosomes, where it recruits and activates the Arp2/3 complex to induce actin polymerization, playing a key role in the fission of tubules that serve as transport intermediates during endosome sorting (PubMed:19922874, PubMed:19922875, PubMed:20498093, PubMed:23452853). Its assembly in the WASH core complex seems to inhibit its NPF activity and via WASHC2 is required for its membrane targeting (PubMed:20498093). Involved in endocytic trafficking of EGF (By similarity). Involved in transferrin receptor recycling. Regulates the trafficking of endosomal alpha5beta1 integrin to the plasma membrane and involved in invasive cell migration (PubMed:22114305). In T-cells involved in endosome-to-membrane recycling of receptors including T-cell receptor (TCR), CD28 and ITGAL; proposed to be implicated in T cell proliferation and effector function. In dendritic cells involved in endosome-to-membrane recycling of major histocompatibility complex (MHC) class II probably involving retromer and subsequently allowing antigen sampling, loading and presentation during T-cell activation (By similarity). Involved in Arp2/3 complex-dependent actin assembly driving Salmonella typhimurium invasion independent of ruffling. Involved in the exocytosis of MMP14 leading to matrix remodeling during invasive migration and implicating late endosome-to-plasma membrane tubular connections and cooperation with the exocyst complex (PubMed:24344185). Involved in negative regulation of autophagy independently from its role in endosomal sorting by inhibiting BECN1 ubiquitination to inactivate PIK3C3/Vps34 activity (By similarity). {ECO:0000250|UniProtKB:C4AMC7, ECO:0000250|UniProtKB:Q8VDD8, ECO:0000269|PubMed:19922874, ECO:0000269|PubMed:19922875, ECO:0000269|PubMed:20498093, ECO:0000269|PubMed:22114305, ECO:0000269|PubMed:23452853, ECO:0000305|PubMed:20498093}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|DUF3462|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|negative regulation of phosphatidylinositol 3-kinase activity|
|negative regulation of lipid kinase activity|
|negative regulation of phospholipid metabolic process|
|invadopodium|
|positive regulation of pseudopodium assembly|
|regulation of pseudopodium assembly|
|WASH complex|
|exocyst|
|Arp2/3 complex-mediated actin nucleation|
|retrograde transport, endosome to plasma membrane|
|actin nucleation|
|alpha-tubulin binding|
|regulation of phosphatidylinositol 3-kinase activity|
|regulation of lipid kinase activity|
|extracellular matrix disassembly|
|autophagosome|
|negative regulation of lipid metabolic process|
|negative regulation of autophagy|
|recycling endosome membrane|
|regulation of phospholipid metabolic process|
|positive regulation of actin filament polymerization|
|vesicle-mediated transport to the plasma membrane|
|retrograde transport, endosome to Golgi|
|positive regulation of plasma membrane bounded cell projection assembly|
|recycling endosome|
|late endosome|
|positive regulation of protein polymerization|
|centriole|
|cytosolic transport|
|early endosome membrane|
|regulation of actin filament polymerization|
|regulation of actin polymerization or depolymerization|
|regulation of actin filament length|
|positive regulation of supramolecular fiber organization|
|regulation of plasma membrane bounded cell projection assembly|
|regulation of protein ubiquitination|
|regulation of cell projection assembly|
|positive regulation of cytoskeleton organization|
|regulation of protein polymerization|
|endosomal transport|
|regulation of protein modification by small protein conjugation or removal|
|actin filament organization|
|negative regulation of kinase activity|
|positive regulation of protein complex assembly|
|negative regulation of cellular catabolic process|
|early endosome|
|regulation of actin filament organization|
|actin binding|
|negative regulation of transferase activity|
|ubiquitin protein ligase binding|
|negative regulation of catabolic process|
|regulation of autophagy|
|extracellular matrix organization|
|regulation of actin cytoskeleton organization|
|regulation of supramolecular fiber organization|
|regulation of cellular component size|
|positive regulation of cell projection organization|
|extracellular structure organization|
|regulation of actin filament-based process|
|regulation of lipid metabolic process|
|cellular component disassembly|
|negative regulation of phosphorylation|
|regulation of protein complex assembly|
|supramolecular fiber organization|
|actin cytoskeleton organization|
|positive regulation of cell migration|
|regulation of anatomical structure size|
|positive regulation of cellular component biogenesis|
|positive regulation of cell motility|
|positive regulation of cellular component movement|
|regulation of cytoskeleton organization|
|positive regulation of locomotion|
|negative regulation of phosphate metabolic process|
|negative regulation of phosphorus metabolic process|
|actin filament-based process|
|positive regulation of organelle organization|
|intracellular membrane-bounded organelle|
|regulation of plasma membrane bounded cell projection organization|
|regulation of cell projection organization|
|negative regulation of catalytic activity|
|exocytosis|
|regulation of cellular catabolic process|
|regulation of cell migration|
|regulation of kinase activity|
|regulation of cell motility|
|regulation of cellular component biogenesis|
|regulation of transferase activity|
|regulation of locomotion|
|regulation of cellular component movement|
|regulation of catabolic process|
|secretion by cell|
|export from cell|
|cytoskeleton organization|
|secretion|
|negative regulation of molecular function|
|positive regulation of cellular component organization|
|regulation of organelle organization|
|protein transport|
|intracellular transport|
|peptide transport|
|amide transport|
|regulation of phosphorylation|
|establishment of protein localization|
|regulation of phosphate metabolic process|
|regulation of phosphorus metabolic process|
|establishment of localization in cell|
|nitrogen compound transport|
|regulation of protein modification process|
|vesicle-mediated transport|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='default' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp19|Etoposide 1μM R00 exp19]]|-1.91|
|[[:results:exp198|Etoposide 0.1μM R05 exp198]]|-1.72|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
No correlation found to any other genes in chemogenomics.
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: N/A
^Tissue^Fraction Of Cell Lines Where Essential^
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 1016
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 5.89 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='WASH1 Expression in NALM6 Cells: 5.89'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>