Vinblastine 0.2μM R03 exp108 [The Human Chemical-Genetic Interaction Map Project]

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====== Vinblastine 0.2μM R03 exp108 ======
==== Mechanism of Action ====
Inhibits microtubule polymerization and mitotic spindle formation, induces spindle assembly checkpoint (SAC)
  * **<color #00a2e8>Class / Subclass 1:</color>** Cell Cycle / Microtubule Poison
  * **<color #00a2e8>Class / Subclass 2:</color>** Organelle Function / Cytoskeletal Inhibitor



==== Technical Notes ====
<accordion collapsed='true'>
<panel title='Compound References' collapsed='true'>
    * **<color #00a2e8>PubChem Name:</color>** %%Vincaleukoblastine sulfate%%
    * **<color #00a2e8>Synonyms:</color>** Vincaleukoblastine sulfate salt
    * **<color #00a2e8>CAS #:</color>** 143-67-9
    * **<color #00a2e8>PubChem CID:</color>** [[https://pubchem.ncbi.nlm.nih.gov/compound/241902|241902]]
    * **<color #00a2e8>IUPAC:</color>** %%methyl (1R,9R,10S,11R,12R,19R)-11-acetyloxy-12-ethyl-4-[(13S,15S,17S)-17-ethyl-17-hydroxy-13-methoxycarbonyl-1,11-diazatetracyclo[13.3.1.04,12.05,10]nonadeca-4(12),5,7,9-tetraen-13-yl]-10-hydroxy-5-methoxy-8-methyl-8,16-diazapentacyclo[10.6.1.01,9.02,7.016,19]nonadeca-2,4,6,13-tetraene-10-carboxylate;sulfuric acid%%
    * **<color #00a2e8>INCHI Name:</color>** InChI=1S/C46H58N4O9.H2O4S/c1-8-42(54)23-28-24-45(40(52)57-6,36-30(15-19-49(25-28)26-42)29-13-10-11-14-33(29)47-36)32-21-31-34(22-35(32)56-5)48(4)38-44(31)17-20-50-18-12-16-43(9-2,37(44)50)39(59-27(3)51)46(38,55)41(53)58-7;1-5(2,3)4/h10-14,16,21-22,28,37-39,47,54-55H,8-9,15,17-20,23-26H2,1-7H3;(H2,1,2,3,4)/t28-,37+,38-,39-,42+,43-,44-,45+,46+;/m1./s1
    * **<color #00a2e8>INCHI Key:</color>** KDQAABAKXDWYSZ-JKDPCDLQSA-N
    * **<color #00a2e8>Molecular Weight:</color>** 909.1
    * **<color #00a2e8>Canonical SMILES:</color>** CCC1(CC2CC(C3=C(CCN(C2)C1)C4=CC=CC=C4N3)(C5=C(C=C6C(=C5)C78CCN9C7C(C=CC9)(C(C(C8N6C)(C(=O)OC)O)OC(=O)C)CC)OC)C(=O)OC)O.OS(=O)(=O)O
    * **<color #00a2e8>Isomeric SMILES:</color>** CC[C@@]1(C[C@@H]2C[C@@](C3=C(CCN(C2)C1)C4=CC=CC=C4N3)(C5=C(C=C6C(=C5)[C@]78CCN9[C@H]7[C@@](C=CC9)([C@H]([C@@]([C@@H]8N6C)(C(=O)OC)O)OC(=O)C)CC)OC)C(=O)OC)O.OS(=O)(=O)O
    * **<color #00a2e8>Molecular Formula:</color>** C46H60N4O13S
{{:chemogenomics:structures:chem-0040.svg?nolink}}
</panel>


<panel title='Compound Supplier' collapsed='true'>
    * **<color #00a2e8>Supplier Name:</color>** Sigma-Aldrich
    * **<color #00a2e8>Catalog #:</color>** V1377
    * **<color #00a2e8>Lot #:</color>** N/A
</panel>


<panel title='Compound Characterization' collapsed='true'>
    * **<color #00a2e8>HRMS (ESI-TOF) m/z:</color>** (M+H)+ Calcd for C46H58N4O9 811.42766; found 811.42916
</panel>


<panel title='Dose Response Curve' collapsed='true'>
    * **<color #00a2e8>Platform ID:</color>** Vinblastine
    * **<color #00a2e8>Min:</color>** -0.8093; **<color #00a2e8>Max:</color>** 78.3626
{{:chemogenomics:dose_response:dr_162.png?nolink&500 |}}
\\ 
\\ 
\\ 
\\ 
|< 300px 100px 200px >|
^ IC   ^ Concentration (µM) ^
| IC10  |N/A    |
| IC20  |0.0461    |
| IC30  |0.1198    |
| IC40  |0.2622    |
| IC50  |0.5380    |
| IC60  |N/A    |
| IC70  |N/A    |
| IC80  |N/A    |
| IC90  |N/A    |
\\ 
</panel>
</accordion>
==== Screen Summary ====
    * **<color #00a2e8>Round</color>**: 03
    * **<color #00a2e8>Dose</color>**: 200nM
    * **<color #00a2e8>Days of incubation</color>**: 8
    * **<color #00a2e8>Doublings</color>**: -0.6
    * **<color #00a2e8>Numbers of reads</color>**: 10228528


==== Screen Results ====


^Sensitive/Resistant hits (FDR<0.05)^CRANKS^Score Plot^Top 30 Genes^Screen Similarity^Top 30 Sensitive GO terms^Top 30 Resistant GO terms^
|0/13|<color #e85500>[[https://files.tyerslab.com/files/public/chemogenomics/cranks/Vinblastine_0.2uM_Round-3_exp108.txt|Scores]]</color>|<button type="primary" size="sm" modal="ScorePlot">View</button>|<button type="primary" size="sm" modal="modal_hits">View</button>|<button type="primary" size="sm" modal="modal_screen_similarity">View</button>|<button type="default" size="sm" modal="modal_go_sensitive">View</button>|<button type="primary" size="sm" modal="modal_go_resistant">View</button>|
<modal id='ScorePlot' size='lg' title='Vinblastine 0.2μM R03 exp108'>
{{:chemogenomics:cranks_plots:exp108.png?nolink}}
</modal>


<modal id="modal_hits" size="sm" title="Top 30 S/R Genes">
^Gene^CRANKS Score^FDR^
|[[:human_genes:S:SART1|SART1]]|-2.76|0.74|
|[[:human_genes:U:UNC50|UNC50]]|-2.57|0.26|
|[[:human_genes:N:NMRAL1|NMRAL1]]|-2.56|0.35|
|[[:human_genes:Y:YTHDC1|YTHDC1]]|-2.34|0.74|
|[[:human_genes:P:PTCH1|PTCH1]]|-2.22|0.63|
|[[:human_genes:L:LMLN|LMLN]]|-2.19|0.58|
|[[:human_genes:C:C10orf111|C10orf111]]|-2.17|0.58|
|[[:human_genes:S:SEPHS2|SEPHS2]]|-2.16|0.58|
|[[:human_genes:A:ANAPC2|ANAPC2]]|-2.11|0.63|
|[[:human_genes:V:VPS52|VPS52]]|-2.10|0.74|
|[[:human_genes:T:TEX13B|TEX13B]]|-2.07|0.63|
|[[:human_genes:T:TBL1XR1|TBL1XR1]]|-2.06|0.63|
|[[:human_genes:O:OLFML2B|OLFML2B]]|-2.05|0.63|
|[[:human_genes:M:MGA|MGA]]|-2.04|0.63|
|[[:human_genes:V:VPS28|VPS28]]|-2.02|0.63|
|[[:human_genes:M:MAEA|MAEA]]|-2.00|0.63|
|[[:human_genes:A:ATP6V0C|ATP6V0C]]|-1.98|0.65|
|[[:human_genes:R:RACGAP1|RACGAP1]]|-1.97|0.63|
|[[:human_genes:C:CCNB1|CCNB1]]|-1.96|0.63|
|[[:human_genes:H:H2BFWT|H2BFWT]]|-1.94|0.63|
|[[:human_genes:L:L3MBTL2|L3MBTL2]]|-1.94|0.63|
|[[:human_genes:F:FCRL2|FCRL2]]|-1.90|0.63|
|[[:human_genes:E:EBP|EBP]]|-1.89|0.63|
|[[:human_genes:Z:ZNF839|ZNF839]]|-1.89|0.63|
|[[:human_genes:T:TBC1D7|TBC1D7]]|-1.88|0.63|
|[[:human_genes:T:TIMM17B|TIMM17B]]|-1.88|0.63|
|[[:human_genes:Z:ZNF335|ZNF335]]|-1.88|0.74|
|[[:human_genes:T:TSPAN14|TSPAN14]]|-1.88|0.63|
|[[:human_genes:T:TSG101|TSG101]]|-1.87|0.63|
|[[:human_genes:S:SRCAP|SRCAP]]|-1.87|0.63|
|[[:human_genes:P:PP2D1|PP2D1]]|2.11|0.16|
|[[:human_genes:U:UBE2G2|UBE2G2]]|2.13|0.16|
|[[:human_genes:P:PAM16|PAM16]]|2.18|0.17|
|[[:human_genes:A:ATP5O|ATP5O]]|2.20|0.12|
|[[:human_genes:T:TRIAP1|TRIAP1]]|2.23|0.16|
|[[:human_genes:M:MED13L|MED13L]]|2.25|0.16|
|[[:human_genes:T:TTI1|TTI1]]|2.25|0.20|
|[[:human_genes:P:PDSS1|PDSS1]]|2.30|0.19|
|[[:human_genes:M:MOCS3|MOCS3]]|2.32|0.18|
|[[:human_genes:R:ROMO1|ROMO1]]|2.32|0.13|
|[[:human_genes:P:PELO|PELO]]|2.33|0.17|
|[[:human_genes:P:PPP6C|PPP6C]]|2.43|0.08|
|[[:human_genes:R:RPTOR|RPTOR]]|2.48|0.03|
|[[:human_genes:M:MS4A13|MS4A13]]|2.54|0.48|
|[[:human_genes:D:DOHH|DOHH]]|2.55|0.05|
|[[:human_genes:R:RTCB|RTCB]]|2.56|0.16|
|[[:human_genes:C:CYCS|CYCS]]|2.57|0.03|
|[[:human_genes:T:TRMT10A|TRMT10A]]|2.60|0.04|
|[[:human_genes:Q:QSER1|QSER1]]|2.67|0.03|
|[[:human_genes:G:GFPT1|GFPT1]]|2.92|0.03|
|[[:human_genes:B:BUB3|BUB3]]|3.04|0.08|
|[[:human_genes:D:DPAGT1|DPAGT1]]|3.28|0.02|
|[[:human_genes:P:PYROXD1|PYROXD1]]|3.35|0.08|
|[[:human_genes:M:MAD2L1BP|MAD2L1BP]]|3.40|<0.01|
|[[:human_genes:Z:ZW10|ZW10]]|3.65|<0.01|
|[[:human_genes:M:MAD1L1|MAD1L1]]|4.41|<0.01|
|[[:human_genes:B:BUB1B|BUB1B]]|5.74|<0.01|
|[[:human_genes:K:KNTC1|KNTC1]]|5.88|<0.01|
|[[:human_genes:M:MAD2L1|MAD2L1]]|6.82|<0.01|
|[[:human_genes:T:TRIP13|TRIP13]]|7.82|<0.01|
</modal>


<modal id="modal_screen_similarity" size="lg" title="Correlated Screens">
^Screen^Correlation^Plot^
|[[:results:exp126|GSK461364A 0.1μM R03 exp126]]|0.141|<button type="primary" size="small" modal="1">View</button>|
|[[:results:exp98|BI-6727 0.04μM R03 exp98]]|0.128|<button type="primary" size="small" modal="2">View</button>|
|[[:results:exp96|BI-2536 0.02μM R03 exp96]]|0.119|<button type="primary" size="small" modal="3">View</button>|
|[[:results:exp111|R-DABN 8μM R03 exp111]]|0.106|<button type="primary" size="small" modal="4">View</button>|
|[[:results:exp448|Ammonium tetrathiomolybdate 10μM R08 exp448]]|0.091|<button type="primary" size="small" modal="5">View</button>|
|[[:results:exp64|Nocodazole 0.2μM R02 exp64]]|0.077|<button type="primary" size="small" modal="6">View</button>|
|[[:results:exp93|DABN racemic mixture R03 exp93]]|0.076|<button type="primary" size="small" modal="7">View</button>|
|[[:results:exp17|DABN 20μM R00 exp17]]|0.071|<button type="primary" size="small" modal="8">View</button>|
|[[:results:exp456|Benzoate 20000μM R08 exp456]]|0.068|<button type="primary" size="small" modal="9">View</button>|
|[[:results:exp539|42°C R08 exp539]]|0.065|<button type="primary" size="small" modal="10">View</button>|
|[[:results:exp256|HMS-I1 10μM R06 exp256]]|0.065|<button type="primary" size="small" modal="11">View</button>|
|[[:results:exp487|Hinokiflavone 12μM R08 exp487]]|0.065|<button type="primary" size="small" modal="12">View</button>|
|[[:results:exp199|Etoposide 0.3μM R05 exp199]]|0.065|<button type="primary" size="small" modal="13">View</button>|
|[[:results:exp443|SNS-032 15μM R08 exp443]]|0.064|<button type="primary" size="small" modal="14">View</button>|
|[[:results:exp472|CI-1040 9.5μM R08 exp472]]|0.063|<button type="primary" size="small" modal="15">View</button>|
|[[:results:exp259|6-Thio-2-deoxyguanosine 2μM R06 exp259]]|0.062|<button type="primary" size="small" modal="16">View</button>|
|[[:results:exp24|Nocodazole 0.2μM R00 exp24]]|0.06|<button type="primary" size="small" modal="17">View</button>|
|[[:results:exp208|Vinblastine 0.015μM R05 exp208]]|0.057|<button type="primary" size="small" modal="18">View</button>|
|[[:results:exp94|Nocodazole 0.1μM R03 exp94]]|0.056|<button type="primary" size="small" modal="19">View</button>|
|[[:results:exp459|Bleomycin 5μM R08 exp459]]|0.055|<button type="primary" size="small" modal="20">View</button>|
|[[:results:exp22|MLN-4924 2μM R00 exp22]]|0.054|<button type="primary" size="small" modal="21">View</button>|
|[[:results:exp478|Doxorubicin 0.02μM R08 exp478]]|0.053|<button type="primary" size="small" modal="22">View</button>|
|[[:results:exp33|Rotenone 2μM R00 exp33]]|0.052|<button type="primary" size="small" modal="23">View</button>|
|[[:results:exp106|UM131593 0.2μM R03 exp106]]|0.051|<button type="primary" size="small" modal="24">View</button>|
|[[:results:exp532|TIC10 10μM R08 exp532]]|0.051|<button type="primary" size="small" modal="25">View</button>|
|[[:results:exp515|PU-H71 1μM R08 exp515]]|0.051|<button type="primary" size="small" modal="26">View</button>|
|[[:results:exp135|MS023 7μM R03 exp135]]|0.051|<button type="primary" size="small" modal="27">View</button>|
</modal>


<modal id="1" size="lg" title="GSK461364A 0.1μM R03 exp126  vs  Vinblastine 0.2μM R03 exp108">
{{:chemogenomics:comparison_plots:exp108_vs_exp126.png?nolink |}}
</modal>

<modal id="2" size="lg" title="BI-6727 0.04μM R03 exp98  vs  Vinblastine 0.2μM R03 exp108">
{{:chemogenomics:comparison_plots:exp108_vs_exp98.png?nolink |}}
</modal>

<modal id="3" size="lg" title="BI-2536 0.02μM R03 exp96  vs  Vinblastine 0.2μM R03 exp108">
{{:chemogenomics:comparison_plots:exp108_vs_exp96.png?nolink |}}
</modal>

<modal id="4" size="lg" title="R-DABN 8μM R03 exp111  vs  Vinblastine 0.2μM R03 exp108">
{{:chemogenomics:comparison_plots:exp108_vs_exp111.png?nolink |}}
</modal>

<modal id="5" size="lg" title="Ammonium tetrathiomolybdate 10μM R08 exp448  vs  Vinblastine 0.2μM R03 exp108">
{{:chemogenomics:comparison_plots:exp108_vs_exp448.png?nolink |}}
</modal>

<modal id="6" size="lg" title="Nocodazole 0.2μM R02 exp64  vs  Vinblastine 0.2μM R03 exp108">
{{:chemogenomics:comparison_plots:exp108_vs_exp64.png?nolink |}}
</modal>

<modal id="7" size="lg" title="DABN racemic mixture R03 exp93  vs  Vinblastine 0.2μM R03 exp108">
{{:chemogenomics:comparison_plots:exp108_vs_exp93.png?nolink |}}
</modal>

<modal id="8" size="lg" title="DABN 20μM R00 exp17  vs  Vinblastine 0.2μM R03 exp108">
{{:chemogenomics:comparison_plots:exp108_vs_exp17.png?nolink |}}
</modal>

<modal id="9" size="lg" title="Benzoate 20000μM R08 exp456  vs  Vinblastine 0.2μM R03 exp108">
{{:chemogenomics:comparison_plots:exp108_vs_exp456.png?nolink |}}
</modal>

<modal id="10" size="lg" title="42°C R08 exp539  vs  Vinblastine 0.2μM R03 exp108">
{{:chemogenomics:comparison_plots:exp108_vs_exp539.png?nolink |}}
</modal>

<modal id="11" size="lg" title="HMS-I1 10μM R06 exp256  vs  Vinblastine 0.2μM R03 exp108">
{{:chemogenomics:comparison_plots:exp108_vs_exp256.png?nolink |}}
</modal>

<modal id="12" size="lg" title="Hinokiflavone 12μM R08 exp487  vs  Vinblastine 0.2μM R03 exp108">
{{:chemogenomics:comparison_plots:exp108_vs_exp487.png?nolink |}}
</modal>

<modal id="13" size="lg" title="Etoposide 0.3μM R05 exp199  vs  Vinblastine 0.2μM R03 exp108">
{{:chemogenomics:comparison_plots:exp108_vs_exp199.png?nolink |}}
</modal>

<modal id="14" size="lg" title="SNS-032 15μM R08 exp443  vs  Vinblastine 0.2μM R03 exp108">
{{:chemogenomics:comparison_plots:exp108_vs_exp443.png?nolink |}}
</modal>

<modal id="15" size="lg" title="CI-1040 9.5μM R08 exp472  vs  Vinblastine 0.2μM R03 exp108">
{{:chemogenomics:comparison_plots:exp108_vs_exp472.png?nolink |}}
</modal>

<modal id="16" size="lg" title="6-Thio-2-deoxyguanosine 2μM R06 exp259  vs  Vinblastine 0.2μM R03 exp108">
{{:chemogenomics:comparison_plots:exp108_vs_exp259.png?nolink |}}
</modal>

<modal id="17" size="lg" title="Nocodazole 0.2μM R00 exp24  vs  Vinblastine 0.2μM R03 exp108">
{{:chemogenomics:comparison_plots:exp108_vs_exp24.png?nolink |}}
</modal>

<modal id="18" size="lg" title="Vinblastine 0.015μM R05 exp208  vs  Vinblastine 0.2μM R03 exp108">
{{:chemogenomics:comparison_plots:exp108_vs_exp208.png?nolink |}}
</modal>

<modal id="19" size="lg" title="Nocodazole 0.1μM R03 exp94  vs  Vinblastine 0.2μM R03 exp108">
{{:chemogenomics:comparison_plots:exp108_vs_exp94.png?nolink |}}
</modal>

<modal id="20" size="lg" title="Bleomycin 5μM R08 exp459  vs  Vinblastine 0.2μM R03 exp108">
{{:chemogenomics:comparison_plots:exp108_vs_exp459.png?nolink |}}
</modal>

<modal id="21" size="lg" title="MLN-4924 2μM R00 exp22  vs  Vinblastine 0.2μM R03 exp108">
{{:chemogenomics:comparison_plots:exp108_vs_exp22.png?nolink |}}
</modal>

<modal id="22" size="lg" title="Doxorubicin 0.02μM R08 exp478  vs  Vinblastine 0.2μM R03 exp108">
{{:chemogenomics:comparison_plots:exp108_vs_exp478.png?nolink |}}
</modal>

<modal id="23" size="lg" title="Rotenone 2μM R00 exp33  vs  Vinblastine 0.2μM R03 exp108">
{{:chemogenomics:comparison_plots:exp108_vs_exp33.png?nolink |}}
</modal>

<modal id="24" size="lg" title="UM131593 0.2μM R03 exp106  vs  Vinblastine 0.2μM R03 exp108">
{{:chemogenomics:comparison_plots:exp106_vs_exp108.png?nolink |}}
</modal>

<modal id="25" size="lg" title="TIC10 10μM R08 exp532  vs  Vinblastine 0.2μM R03 exp108">
{{:chemogenomics:comparison_plots:exp108_vs_exp532.png?nolink |}}
</modal>

<modal id="26" size="lg" title="PU-H71 1μM R08 exp515  vs  Vinblastine 0.2μM R03 exp108">
{{:chemogenomics:comparison_plots:exp108_vs_exp515.png?nolink |}}
</modal>

<modal id="27" size="lg" title="MS023 7μM R03 exp135  vs  Vinblastine 0.2μM R03 exp108">
{{:chemogenomics:comparison_plots:exp108_vs_exp135.png?nolink |}}
</modal>



<modal id="modal_go_sensitive" size="lg" title="Top 30 sensitive GO terms">No GO term hits below threshold. (FDR <0.05)</modal>
<modal id="modal_go_resistant" size="lg" title="Top 30 resistant GO terms">
^GO Term^Fold Change^Genes^
|spindle assembly checkpoint signaling|138.16|TRIP13,MAD2L1,KNTC1,BUB1B,MAD1L1,ZW10,BUB3|
|mitotic spindle assembly checkpoint signaling|138.16|TRIP13,MAD2L1,KNTC1,BUB1B,MAD1L1,ZW10,BUB3|
|mitotic spindle checkpoint signaling|138.16|TRIP13,MAD2L1,KNTC1,BUB1B,MAD1L1,ZW10,BUB3|
|negative regulation of mitotic sister chromatid separation|124.79|TRIP13,MAD2L1,KNTC1,BUB1B,MAD1L1,ZW10,BUB3|
|negative regulation of mitotic sister chromatid segregation|124.79|TRIP13,MAD2L1,KNTC1,BUB1B,MAD1L1,ZW10,BUB3|
|negative regulation of metaphase/anaphase transition of cell cycle|117.23|TRIP13,MAD2L1,KNTC1,BUB1B,MAD1L1,ZW10,BUB3|
|negative regulation of chromosome segregation|117.23|TRIP13,MAD2L1,KNTC1,BUB1B,MAD1L1,ZW10,BUB3|
|negative regulation of chromosome separation|117.23|TRIP13,MAD2L1,KNTC1,BUB1B,MAD1L1,ZW10,BUB3|
|spindle checkpoint signaling|114.34|TRIP13,KNTC1,BUB1B,MAD1L1,ZW10,BUB3|
|negative regulation of mitotic metaphase/anaphase transition|106.96|TRIP13,MAD2L1,KNTC1,BUB1B,MAD1L1,ZW10|
|negative regulation of sister chromatid segregation|106.96|TRIP13,KNTC1,BUB1B,MAD1L1,ZW10,BUB3|
|negative regulation of nuclear division|84.10|TRIP13,MAD2L1,KNTC1,BUB1B,MAD1L1,ZW10,BUB3|
|regulation of mitotic sister chromatid segregation|83.42|TRIP13,MAD2L1,KNTC1,BUB1B,MAD1L1,ZW10,MAD2L1BP,BUB3|
|regulation of mitotic sister chromatid separation|66.70|TRIP13,MAD2L1,BUB1B,MAD1L1,ZW10,MAD2L1BP,BUB3|
|regulation of chromosome separation|60.56|TRIP13,MAD2L1,KNTC1,BUB1B,MAD1L1,ZW10,MAD2L1BP,BUB3|
|regulation of mitotic metaphase/anaphase transition|49.67|TRIP13,MAD2L1,KNTC1,BUB1B,MAD1L1,ZW10,MAD2L1BP,BUB3|
|regulation of metaphase/anaphase transition of cell cycle|48.06|TRIP13,MAD2L1,KNTC1,BUB1B,MAD1L1,ZW10,MAD2L1BP,BUB3|
|regulation of sister chromatid segregation|42.92|TRIP13,MAD2L1,KNTC1,BUB1B,MAD1L1,ZW10,MAD2L1BP,BUB3|
|regulation of mitotic nuclear division|37.79|TRIP13,MAD2L1,KNTC1,BUB1B,MAD1L1,ZW10,MAD2L1BP,BUB3|
</modal>