GSK461364A 0.005μM R03 exp125 [The Human Chemical-Genetic Interaction Map Project]

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====== GSK461364A 0.005μM R03 exp125 ======
==== Mechanism of Action ====
Inhibits PLK1
  * **<color #00a2e8>Class / Subclass 1:</color>** Signal Transduction / Kinase Inhibitor
  * **<color #00a2e8>Class / Subclass 2:</color>** Cell Cycle / Mitotic Inhibitor



==== Technical Notes ====
<accordion collapsed='true'>
<panel title='Compound References' collapsed='true'>
    * **<color #00a2e8>PubChem Name:</color>** %%Gsk-461364%%
    * **<color #00a2e8>Synonyms:</color>** GSK461364A
    * **<color #00a2e8>CAS #:</color>** 929095-18-1
    * **<color #00a2e8>PubChem CID:</color>** [[https://pubchem.ncbi.nlm.nih.gov/compound/15983966|15983966]]
    * **<color #00a2e8>IUPAC:</color>** %%5-[6-[(4-methylpiperazin-1-yl)methyl]benzimidazol-1-yl]-3-[(1R)-1-[2-(trifluoromethyl)phenyl]ethoxy]thiophene-2-carboxamide%%
    * **<color #00a2e8>INCHI Name:</color>** InChI=1S/C27H28F3N5O2S/c1-17(19-5-3-4-6-20(19)27(28,29)30)37-23-14-24(38-25(23)26(31)36)35-16-32-21-8-7-18(13-22(21)35)15-34-11-9-33(2)10-12-34/h3-8,13-14,16-17H,9-12,15H2,1-2H3,(H2,31,36)/t17-/m1/s1
    * **<color #00a2e8>INCHI Key:</color>** ZHJGWYRLJUCMRT-QGZVFWFLSA-N
    * **<color #00a2e8>Molecular Weight:</color>** 543.6
    * **<color #00a2e8>Canonical SMILES:</color>** CC(C1=CC=CC=C1C(F)(F)F)OC2=C(SC(=C2)N3C=NC4=C3C=C(C=C4)CN5CCN(CC5)C)C(=O)N
    * **<color #00a2e8>Isomeric SMILES:</color>** C[C@H](C1=CC=CC=C1C(F)(F)F)OC2=C(SC(=C2)N3C=NC4=C3C=C(C=C4)CN5CCN(CC5)C)C(=O)N
    * **<color #00a2e8>Molecular Formula:</color>** C27H28F3N5O2S
{{:chemogenomics:structures:chem-0078.svg?nolink}}
</panel>


<panel title='Compound Supplier' collapsed='true'>
    * **<color #00a2e8>Supplier Name:</color>** Med Chem Express
    * **<color #00a2e8>Catalog #:</color>** HY-50877
    * **<color #00a2e8>Lot #:</color>** CS-0069-03047
</panel>


<panel title='Compound Characterization' collapsed='true'>
    * **<color #00a2e8>HRMS (ESI-TOF) m/z:</color>** (M+H)+ Calcd for C27H28F3N5O2S 544.19886; found 544.20114
</panel>


<panel title='Dose Response Curve' collapsed='true'>
    * **<color #00a2e8>Platform ID:</color>** GSK461364A
    * **<color #00a2e8>Min:</color>** 0.3593; **<color #00a2e8>Max:</color>** 95.3469
{{:chemogenomics:dose_response:dr_83.png?nolink&500 |}}
\\ 
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|< 300px 100px 200px >|
^ IC   ^ Concentration (µM) ^
| IC10  |N/A    |
| IC20  |N/A    |
| IC30  |0.0022    |
| IC40  |0.0102    |
| IC50  |0.0421    |
| IC60  |N/A    |
| IC70  |N/A    |
| IC80  |N/A    |
| IC90  |N/A    |
\\ 
</panel>
</accordion>
==== Screen Summary ====
    * **<color #00a2e8>Round</color>**: 03
    * **<color #00a2e8>Dose</color>**: 5nM
    * **<color #00a2e8>Days of incubation</color>**: 8
    * **<color #00a2e8>Doublings</color>**: 6.5
    * **<color #00a2e8>Numbers of reads</color>**: 13142837


==== Screen Results ====


^Sensitive/Resistant hits (FDR<0.05)^CRANKS^Score Plot^Top 30 Genes^Screen Similarity^Top 30 Sensitive GO terms^Top 30 Resistant GO terms^
|23/0|<color #e85500>[[https://files.tyerslab.com/files/public/chemogenomics/cranks/GSK461364A_0.005uM_Round-3_exp125.txt|Scores]]</color>|<button type="primary" size="sm" modal="ScorePlot">View</button>|<button type="primary" size="sm" modal="modal_hits">View</button>|<button type="primary" size="sm" modal="modal_screen_similarity">View</button>|<button type="primary" size="sm" modal="modal_go_sensitive">View</button>|<button type="default" size="sm" modal="modal_go_resistant">View</button>|
<modal id='ScorePlot' size='lg' title='GSK461364A 0.005μM R03 exp125'>
{{:chemogenomics:cranks_plots:exp125.png?nolink}}
</modal>


<modal id="modal_hits" size="sm" title="Top 30 S/R Genes">
^Gene^CRANKS Score^FDR^
|[[:human_genes:K:KIF18A|KIF18A]]|-5.81|<0.01|
|[[:human_genes:C:CENPB|CENPB]]|-3.93|<0.01|
|[[:human_genes:M:MLF1IP|MLF1IP]]|-3.87|<0.01|
|[[:human_genes:P:PDS5B|PDS5B]]|-3.68|<0.01|
|[[:human_genes:R:RALGAPB|RALGAPB]]|-3.38|<0.01|
|[[:human_genes:I:IVNS1ABP|IVNS1ABP]]|-3.19|<0.01|
|[[:human_genes:L:LCMT1|LCMT1]]|-3.19|<0.01|
|[[:human_genes:S:SKA1|SKA1]]|-3.18|<0.01|
|[[:human_genes:P:PRR14L|PRR14L]]|-3.08|<0.01|
|[[:human_genes:A:ACTR1B|ACTR1B]]|-2.93|<0.01|
|[[:human_genes:D:DPH1|DPH1]]|-2.92|<0.01|
|[[:human_genes:U:UBE4B|UBE4B]]|-2.84|<0.01|
|[[:human_genes:D:DPH5|DPH5]]|-2.81|<0.01|
|[[:human_genes:G:GCLC|GCLC]]|-2.78|0.01|
|[[:human_genes:A:ACTR1A|ACTR1A]]|-2.77|<0.01|
|[[:human_genes:S:SIN3B|SIN3B]]|-2.65|0.01|
|[[:human_genes:K:KIF15|KIF15]]|-2.61|0.01|
|[[:human_genes:R:RBM38|RBM38]]|-2.58|0.01|
|[[:human_genes:C:CENPQ|CENPQ]]|-2.57|0.01|
|[[:human_genes:C:CENPI|CENPI]]|-2.54|0.19|
|[[:human_genes:K:KLF16|KLF16]]|-2.54|0.07|
|[[:human_genes:E:ELAVL1|ELAVL1]]|-2.53|0.02|
|[[:human_genes:N:N6AMT1|N6AMT1]]|-2.52|0.11|
|[[:human_genes:D:DDX39A|DDX39A]]|-2.48|0.02|
|[[:human_genes:C:CENPA|CENPA]]|-2.46|0.05|
|[[:human_genes:C:CENPL|CENPL]]|-2.44|0.05|
|[[:human_genes:C:CCNB1|CCNB1]]|-2.43|0.03|
|[[:human_genes:C:CENPM|CENPM]]|-2.37|0.43|
|[[:human_genes:N:NPRL2|NPRL2]]|-2.37|0.07|
|[[:human_genes:T:TPX2|TPX2]]|-2.34|0.19|
|[[:human_genes:M:MAD1L1|MAD1L1]]|2.03|0.35|
|[[:human_genes:U:UBE2J1|UBE2J1]]|2.04|0.26|
|[[:human_genes:P:PSMC3|PSMC3]]|2.04|0.60|
|[[:human_genes:M:METTL5|METTL5]]|2.05|0.26|
|[[:human_genes:D:DNAJB9|DNAJB9]]|2.06|0.26|
|[[:human_genes:B:BIRC6|BIRC6]]|2.07|0.26|
|[[:human_genes:S:SUMO1|SUMO1]]|2.08|0.49|
|[[:human_genes:T:TMEM55B|TMEM55B]]|2.09|0.26|
|[[:human_genes:I:IQCD|IQCD]]|2.09|0.26|
|[[:human_genes:C:CCDC63|CCDC63]]|2.10|0.26|
|[[:human_genes:A:APOH|APOH]]|2.11|0.26|
|[[:human_genes:P:POLR1E|POLR1E]]|2.13|0.26|
|[[:human_genes:G:GGA3|GGA3]]|2.14|0.28|
|[[:human_genes:S:SLU7|SLU7]]|2.15|0.47|
|[[:human_genes:C:C2orf66|C2orf66]]|2.16|0.26|
|[[:human_genes:P:PPM1G|PPM1G]]|2.17|0.26|
|[[:human_genes:P:PPM1A|PPM1A]]|2.18|0.26|
|[[:human_genes:C:CDC45|CDC45]]|2.20|0.63|
|[[:human_genes:P:POLD3|POLD3]]|2.20|0.66|
|[[:human_genes:R:RPS23|RPS23]]|2.22|0.26|
|[[:human_genes:E:ENY2|ENY2]]|2.22|0.26|
|[[:human_genes:R:RPL18A|RPL18A]]|2.25|0.26|
|[[:human_genes:R:RND3|RND3]]|2.27|0.25|
|[[:human_genes:R:RASA1|RASA1]]|2.28|0.26|
|[[:human_genes:T:TFAP4|TFAP4]]|2.38|0.16|
|[[:human_genes:S:SYNCRIP|SYNCRIP]]|2.43|0.30|
|[[:human_genes:O:OR5K3|OR5K3]]|2.55|0.16|
|[[:human_genes:O:OR3A2|OR3A2]]|2.56|0.16|
|[[:human_genes:X:XPR1|XPR1]]|2.68|0.11|
|[[:human_genes:G:GIPR|GIPR]]|2.78|0.11|
</modal>


<modal id="modal_screen_similarity" size="lg" title="Correlated Screens">
^Screen^Correlation^Plot^
|[[:results:exp190|Vincristine 0.0005μM R04 exp190]]|0.07|<button type="primary" size="small" modal="1">View</button>|
|[[:results:exp243|S-trityl-L-cysteine 0.5μM R05 exp243]]|0.051|<button type="primary" size="small" modal="2">View</button>|
|[[:results:exp95|BI-2536 0.0042μM R03 exp95]]|0.129|<button type="primary" size="small" modal="3">View</button>|
|[[:results:exp97|BI-6727 0.0125μM R03 exp97]]|0.119|<button type="primary" size="small" modal="4">View</button>|
|[[:results:exp162|BI-D1870 2μM R04 exp162]]|0.06|<button type="primary" size="small" modal="5">View</button>|
|[[:results:exp228|Demecolcine 0.03μM R05 exp228]]|0.058|<button type="primary" size="small" modal="6">View</button>|
|[[:results:exp179|Combretastatin A4 0.002 to 0.003μM day4 R04 exp179]]|0.056|<button type="primary" size="small" modal="7">View</button>|
</modal>


<modal id="1" size="lg" title="GSK461364A 0.005μM R03 exp125  vs  Vincristine 0.0005μM R04 exp190">
{{:chemogenomics:comparison_plots:exp125_vs_exp190.png?nolink |}}
</modal>

<modal id="2" size="lg" title="GSK461364A 0.005μM R03 exp125  vs  S-trityl-L-cysteine 0.5μM R05 exp243">
{{:chemogenomics:comparison_plots:exp125_vs_exp243.png?nolink |}}
</modal>

<modal id="3" size="lg" title="BI-2536 0.0042μM R03 exp95  vs  GSK461364A 0.005μM R03 exp125">
{{:chemogenomics:comparison_plots:exp125_vs_exp95.png?nolink |}}
</modal>

<modal id="4" size="lg" title="BI-6727 0.0125μM R03 exp97  vs  GSK461364A 0.005μM R03 exp125">
{{:chemogenomics:comparison_plots:exp125_vs_exp97.png?nolink |}}
</modal>

<modal id="5" size="lg" title="BI-D1870 2μM R04 exp162  vs  GSK461364A 0.005μM R03 exp125">
{{:chemogenomics:comparison_plots:exp125_vs_exp162.png?nolink |}}
</modal>

<modal id="6" size="lg" title="Demecolcine 0.03μM R05 exp228  vs  GSK461364A 0.005μM R03 exp125">
{{:chemogenomics:comparison_plots:exp125_vs_exp228.png?nolink |}}
</modal>

<modal id="7" size="lg" title="Combretastatin A4 0.002 to 0.003μM day4 R04 exp179  vs  GSK461364A 0.005μM R03 exp125">
{{:chemogenomics:comparison_plots:exp125_vs_exp179.png?nolink |}}
</modal>



<modal id="modal_go_sensitive" size="lg" title="Top 30 sensitive GO terms">
^GO Term^Fold Change^Genes^
|inner kinetochore|139.32|CENPU,CENPQ,CENPL,CENPM|
|condensed chromosome, centromeric region|33.83|KIF18A,CENPB,CENPU,SKA1,CENPQ,CENPI,CENPA,CENPL,CCNB1,CENPM|
|kinetochore|28.88|KIF18A,CENPU,SKA1,CENPQ,CENPI,CENPL,CCNB1,CENPM|
|chromosome, centromeric region|27.60|KIF18A,CENPB,CENPU,PDS5B,SKA1,CENPQ,CENPI,CENPA,CENPL,CCNB1,CENPM|
|condensed chromosome|21.77|KIF18A,CENPB,CENPU,SKA1,CENPQ,CENPI,CENPA,CENPL,CCNB1,CENPM|
|chromosomal region|17.41|KIF18A,CENPB,CENPU,PDS5B,SKA1,CENPQ,CENPI,CENPA,CENPL,CCNB1,CENPM|
|chromosome segregation|14.44|KIF18A,CENPU,SKA1,CENPQ,CENPI,CENPL,CCNB1,CENPM|
|cell cycle process|7.46|KIF18A,CENPU,PDS5B,LCMT1,SKA1,CENPQ,CENPI,CENPA,CENPL,CCNB1,CENPM|
</modal>
<modal id="modal_go_resistant" size="lg" title="Top 30 resistant GO terms">No GO term hits below threshold. (FDR <0.05)</modal>