Ribavirin 10 to 15μM on day4 R04 exp160 [The Human Chemical-Genetic Interaction Map Project]

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====== Ribavirin 10 to 15μM on day4 R04 exp160 ======
==== Mechanism of Action ====
Prodrug metabolized to purine RNA nucleotide mimetic, also inhibits EIF4E
  * **<color #00a2e8>Class / Subclass 1:</color>** Infectious Disease / Antiviral
  * **<color #00a2e8>Class / Subclass 2:</color>** Metabolism / Antimetabolite
  * **<color #00a2e8>Class / Subclass 3:</color>** DNA Damage, Repair and Replication / Reverse Transcriptase Inhibitor


==== Technical Notes ====
<accordion collapsed='true'>
<panel title='Compound References' collapsed='true'>
    * **<color #00a2e8>PubChem Name:</color>** %%Ribavirin%%
    * **<color #00a2e8>Synonyms:</color>** ICN-1229
    * **<color #00a2e8>CAS #:</color>** 36791-04-5
    * **<color #00a2e8>PubChem CID:</color>** [[https://pubchem.ncbi.nlm.nih.gov/compound/37542|37542]]
    * **<color #00a2e8>IUPAC:</color>** %%1-[(2R,3R,4S,5R)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]-1,2,4-triazole-3-carboxamide%%
    * **<color #00a2e8>INCHI Name:</color>** InChI=1S/C8H12N4O5/c9-6(16)7-10-2-12(11-7)8-5(15)4(14)3(1-13)17-8/h2-5,8,13-15H,1H2,(H2,9,16)/t3-,4-,5-,8-/m1/s1
    * **<color #00a2e8>INCHI Key:</color>** IWUCXVSUMQZMFG-AFCXAGJDSA-N
    * **<color #00a2e8>Molecular Weight:</color>** 244.2
    * **<color #00a2e8>Canonical SMILES:</color>** C1=NC(=NN1C2C(C(C(O2)CO)O)O)C(=O)N
    * **<color #00a2e8>Isomeric SMILES:</color>** C1=NC(=NN1[C@H]2[C@@H]([C@@H]([C@H](O2)CO)O)O)C(=O)N
    * **<color #00a2e8>Molecular Formula:</color>** C8H12N4O5
{{:chemogenomics:structures:chem-0032.svg?nolink}}
</panel>


<panel title='Compound Supplier' collapsed='true'>
    * **<color #00a2e8>Supplier Name:</color>** Kemprotec Ltd
    * **<color #00a2e8>Catalog #:</color>** K-1104
    * **<color #00a2e8>Lot #:</color>** 170221
</panel>


<panel title='Compound Characterization' collapsed='true'>
Characterization data not available.
</panel>


<panel title='Dose Response Curve' collapsed='true'>
    * **<color #00a2e8>Platform ID:</color>** Ribavirin
    * **<color #00a2e8>Min:</color>** -21.0950; **<color #00a2e8>Max:</color>** 37.2497
{{:chemogenomics:dose_response:dr_117.png?nolink&500 |}}
\\ 
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|< 300px 100px 200px >|
^ IC   ^ Concentration (µM) ^
| IC10  |N/A    |
| IC20  |30.6995    |
| IC30  |36.4591    |
| IC40  |N/A    |
| IC50  |N/A    |
| IC60  |N/A    |
| IC70  |N/A    |
| IC80  |N/A    |
| IC90  |N/A    |
\\ 
</panel>
</accordion>
==== Screen Summary ====
    * **<color #00a2e8>Round</color>**: 04
    * **<color #00a2e8>Dose</color>**: 10-15µM
    * **<color #00a2e8>Days of incubation</color>**: 8
    * **<color #00a2e8>Doublings</color>**: 6.6
    * **<color #00a2e8>Numbers of reads</color>**: 14345761


==== Screen Results ====


^Sensitive/Resistant hits (FDR<0.05)^CRANKS^Score Plot^Top 30 Genes^Screen Similarity^Top 30 Sensitive GO terms^Top 30 Resistant GO terms^
|18/8|<color #e85500>[[https://files.tyerslab.com/files/public/chemogenomics/cranks/Ribavirin_10-15day4uM_Round-4_exp160.txt|Scores]]</color>|<button type="primary" size="sm" modal="ScorePlot">View</button>|<button type="primary" size="sm" modal="modal_hits">View</button>|<button type="primary" size="sm" modal="modal_screen_similarity">View</button>|<button type="primary" size="sm" modal="modal_go_sensitive">View</button>|<button type="primary" size="sm" modal="modal_go_resistant">View</button>|
<modal id='ScorePlot' size='lg' title='Ribavirin 10 to 15μM on day4 R04 exp160'>
{{:chemogenomics:cranks_plots:exp160.png?nolink}}
</modal>


<modal id="modal_hits" size="sm" title="Top 30 S/R Genes">
^Gene^CRANKS Score^FDR^
|[[:human_genes:A:AMPD2|AMPD2]]|-5.11|<0.01|
|[[:human_genes:S:SEPSECS|SEPSECS]]|-3.15|<0.01|
|[[:human_genes:P:PRPS1|PRPS1]]|-3.11|<0.01|
|[[:human_genes:G:GPR107|GPR107]]|-3.05|<0.01|
|[[:human_genes:S:SUMO2|SUMO2]]|-2.91|0.11|
|[[:human_genes:A:ARL1|ARL1]]|-2.80|0.01|
|[[:human_genes:R:RALA|RALA]]|-2.71|0.02|
|[[:human_genes:M:MBTPS1|MBTPS1]]|-2.69|0.05|
|[[:human_genes:K:KDSR|KDSR]]|-2.65|0.02|
|[[:human_genes:P:PCGF6|PCGF6]]|-2.64|0.02|
|[[:human_genes:H:HPRT1|HPRT1]]|-2.59|0.04|
|[[:human_genes:F:FBXW7|FBXW7]]|-2.58|0.03|
|[[:human_genes:T:TCF4|TCF4]]|-2.56|0.03|
|[[:human_genes:K:KMT2D|KMT2D]]|-2.55|0.03|
|[[:human_genes:S:SIVA1|SIVA1]]|-2.47|0.03|
|[[:human_genes:G:GCLC|GCLC]]|-2.46|0.03|
|[[:human_genes:L:LAMTOR4|LAMTOR4]]|-2.43|0.04|
|[[:human_genes:Z:ZFYVE20|ZFYVE20]]|-2.40|0.13|
|[[:human_genes:A:ATP6V1G1|ATP6V1G1]]|-2.40|0.08|
|[[:human_genes:E:EIF4A1|EIF4A1]]|-2.38|0.05|
|[[:human_genes:E:EXOC2|EXOC2]]|-2.37|0.05|
|[[:human_genes:P:PRRC2C|PRRC2C]]|-2.35|0.15|
|[[:human_genes:X:XPOT|XPOT]]|-2.28|0.11|
|[[:human_genes:T:TRAPPC3|TRAPPC3]]|-2.27|0.08|
|[[:human_genes:A:ATP2A2|ATP2A2]]|-2.25|0.26|
|[[:human_genes:R:RAET1G|RAET1G]]|-2.20|0.21|
|[[:human_genes:L:LACC1|LACC1]]|-2.20|0.11|
|[[:human_genes:I:IVNS1ABP|IVNS1ABP]]|-2.17|0.11|
|[[:human_genes:S:SEPHS1|SEPHS1]]|-2.16|0.11|
|[[:human_genes:T:TEN1|TEN1]]|-2.16|0.11|
|[[:human_genes:C:C10orf2|C10orf2]]|2.15|0.16|
|[[:human_genes:C:COX18|COX18]]|2.15|0.16|
|[[:human_genes:F:FAM129C|FAM129C]]|2.15|0.20|
|[[:human_genes:Z:ZNF317|ZNF317]]|2.17|0.20|
|[[:human_genes:N:NFU1|NFU1]]|2.18|0.31|
|[[:human_genes:U:UQCC1|UQCC1]]|2.18|0.20|
|[[:human_genes:T:TRUB1|TRUB1]]|2.20|0.14|
|[[:human_genes:A:APOBEC3F|APOBEC3F]]|2.22|0.19|
|[[:human_genes:C:CD1C|CD1C]]|2.25|0.17|
|[[:human_genes:A:ATP6AP2|ATP6AP2]]|2.26|0.21|
|[[:human_genes:C:COQ4|COQ4]]|2.27|0.11|
|[[:human_genes:D:DDX54|DDX54]]|2.29|0.20|
|[[:human_genes:M:MRPL48|MRPL48]]|2.32|0.23|
|[[:human_genes:F:FOXRED1|FOXRED1]]|2.34|0.08|
|[[:human_genes:S:SHD|SHD]]|2.38|0.07|
|[[:human_genes:C:COX15|COX15]]|2.42|0.06|
|[[:human_genes:P:PIK3C2A|PIK3C2A]]|2.43|0.06|
|[[:human_genes:N:NDUFA5|NDUFA5]]|2.49|0.05|
|[[:human_genes:N:NDUFAF3|NDUFAF3]]|2.52|0.13|
|[[:human_genes:C:COX6C|COX6C]]|2.53|0.19|
|[[:human_genes:A:ADSS|ADSS]]|2.54|0.23|
|[[:human_genes:R:RPL30|RPL30]]|2.56|0.45|
|[[:human_genes:N:NUBPL|NUBPL]]|2.60|0.04|
|[[:human_genes:Y:YBEY|YBEY]]|2.60|0.06|
|[[:human_genes:C:CDK13|CDK13]]|2.74|0.04|
|[[:human_genes:P:PET117|PET117]]|2.78|0.01|
|[[:human_genes:N:NDUFA2|NDUFA2]]|3.02|0.01|
|[[:human_genes:M:MTO1|MTO1]]|3.43|<0.01|
|[[:human_genes:N:NUDT2|NUDT2]]|5.35|<0.01|
|[[:human_genes:A:ADK|ADK]]|8.18|<0.01|
</modal>


<modal id="modal_screen_similarity" size="lg" title="Correlated Screens">
^Screen^Correlation^Plot^
|[[:results:exp52|Ribavirin 10μM R01 exp52]]|0.06|<button type="primary" size="small" modal="1">View</button>|
|[[:results:exp288|HMS-I2 10μM R06 exp288]]|0.066|<button type="primary" size="small" modal="2">View</button>|
|[[:results:exp360|Genistein 15μM R07 exp360]]|0.055|<button type="primary" size="small" modal="3">View</button>|
|[[:results:exp25|Oligomycin-A 2μM R00 exp25]]|0.055|<button type="primary" size="small" modal="4">View</button>|
|[[:results:exp169|BH1 1μM R04 exp169]]|0.051|<button type="primary" size="small" modal="5">View</button>|
</modal>


<modal id="1" size="lg" title="Ribavirin 10 to 15μM on day4 R04 exp160  vs  Ribavirin 10μM R01 exp52">
{{:chemogenomics:comparison_plots:exp160_vs_exp52.png?nolink |}}
</modal>

<modal id="2" size="lg" title="HMS-I2 10μM R06 exp288  vs  Ribavirin 10 to 15μM on day4 R04 exp160">
{{:chemogenomics:comparison_plots:exp160_vs_exp288.png?nolink |}}
</modal>

<modal id="3" size="lg" title="Genistein 15μM R07 exp360  vs  Ribavirin 10 to 15μM on day4 R04 exp160">
{{:chemogenomics:comparison_plots:exp160_vs_exp360.png?nolink |}}
</modal>

<modal id="4" size="lg" title="Oligomycin-A 2μM R00 exp25  vs  Ribavirin 10 to 15μM on day4 R04 exp160">
{{:chemogenomics:comparison_plots:exp160_vs_exp25.png?nolink |}}
</modal>

<modal id="5" size="lg" title="BH1 1μM R04 exp169  vs  Ribavirin 10 to 15μM on day4 R04 exp160">
{{:chemogenomics:comparison_plots:exp160_vs_exp169.png?nolink |}}
</modal>



<modal id="modal_go_sensitive" size="lg" title="Top 30 sensitive GO terms">
^GO Term^Fold Change^Genes^
|hypoxanthine biosynthetic process|571.52|PRPS1,HPRT1|
</modal>
<modal id="modal_go_resistant" size="lg" title="Top 30 resistant GO terms">
^GO Term^Fold Change^Genes^
|cytochrome complex assembly|65.00|PET117,COX15,UQCC1,COX18|
|mitochondrial respiratory chain complex I assembly|63.75|NDUFA2,NUBPL,NDUFAF3,NDUFA5,FOXRED1,NDUFA9|
|NADH dehydrogenase complex assembly|63.75|NDUFA2,NUBPL,NDUFAF3,NDUFA5,FOXRED1,NDUFA9|
|mitochondrial respiratory chain complex assembly|57.15|NDUFA2,PET117,NUBPL,NDUFAF3,NDUFA5,FOXRED1,UQCC1,NDUFA9,COX18|
|mitochondrial respirasome|44.20|NDUFA2,COX6C,NDUFA5,COX15,FOXRED1,NDUFA9|
|respirasome|40.42|NDUFA2,COX6C,NDUFA5,COX15,FOXRED1,NDUFA9|
|aerobic electron transport chain|37.84|NDUFA2,COX6C,NDUFA5,COX15,NDUFA9|
|respiratory chain complex|37.33|NDUFA2,COX6C,NDUFA5,FOXRED1,NDUFA9|
|mitochondrial ATP synthesis coupled electron transport|36.35|NDUFA2,COX6C,NDUFA5,COX15,NDUFA9|
|purine nucleoside triphosphate biosynthetic process|34.10|ADK,NUDT2,NDUFA2,NDUFA5,NDUFA9|
|nucleoside triphosphate biosynthetic process|29.70|ADK,NUDT2,NDUFA2,NDUFA5,NDUFA9|
|aerobic respiration|23.51|NDUFA2,COX6C,NDUFA5,COX15,NDUFA9,MDH2|
|cellular respiration|19.61|NDUFA2,COX6C,NDUFA5,COX15,NDUFA9,MDH2|
|purine ribonucleotide biosynthetic process|19.05|ADK,NUDT2,NDUFA2,ADSS2,NDUFA5,NDUFA9|
|mitochondrial inner membrane|13.27|NDUFA2,COX6C,NDUFAF3,NDUFA5,COX15,FOXRED1,MRPL48,COQ4,UQCC1,NDUFA9,COX18|
|organelle inner membrane|11.92|NDUFA2,COX6C,NDUFAF3,NDUFA5,COX15,FOXRED1,MRPL48,COQ4,UQCC1,NDUFA9,COX18|
|mitochondrion organization|11.84|NDUFA2,PET117,NUBPL,NDUFAF3,NDUFA5,FOXRED1,UQCC1,NDUFA9,COX18|
|mitochondrial membrane|8.62|NDUFA2,COX6C,NDUFAF3,NDUFA5,COX15,FOXRED1,MRPL48,COQ4,UQCC1,NDUFA9,COX18|
|mitochondrial envelope|8.09|NDUFA2,COX6C,NDUFAF3,NDUFA5,COX15,FOXRED1,MRPL48,COQ4,UQCC1,NDUFA9,COX18|
</modal>