Temozolomide 200μM R04 exp189 [The Human Chemical-Genetic Interaction Map Project]

This page is read only. You can view the source, but not change it. Ask your administrator if you think this is wrong.
====== Temozolomide 200μM R04 exp189 ======
==== Mechanism of Action ====
Alkylates purine bases, prodrug hydrolyzes to 3-methyl-(triazen-1-yl)imidazole-4-carboxamide (MTIC)
  * **<color #00a2e8>Class / Subclass 1:</color>** DNA Damage, Repair and Replication / Base Alkylation




==== Technical Notes ====
<accordion collapsed='true'>
<panel title='Compound References' collapsed='true'>
    * **<color #00a2e8>PubChem Name:</color>** %%Temozolomide%%
    * **<color #00a2e8>Synonyms:</color>** NSC 362856; CCRG 81045; TMZ; NSC 362856;CCRG 81045;TMZ
    * **<color #00a2e8>CAS #:</color>** 85622-93-1
    * **<color #00a2e8>PubChem CID:</color>** [[https://pubchem.ncbi.nlm.nih.gov/compound/5394|5394]]
    * **<color #00a2e8>IUPAC:</color>** %%3-methyl-4-oxoimidazo[5,1-d][1,2,3,5]tetrazine-8-carboxamide%%
    * **<color #00a2e8>INCHI Name:</color>** InChI=1S/C6H6N6O2/c1-11-6(14)12-2-8-3(4(7)13)5(12)9-10-11/h2H,1H3,(H2,7,13)
    * **<color #00a2e8>INCHI Key:</color>** BPEGJWRSRHCHSN-UHFFFAOYSA-N
    * **<color #00a2e8>Molecular Weight:</color>** 194.15
    * **<color #00a2e8>Canonical SMILES:</color>** CN1C(=O)N2C=NC(=C2N=N1)C(=O)N
    * **<color #00a2e8>Isomeric SMILES:</color>** N/A
    * **<color #00a2e8>Molecular Formula:</color>** C6H6N6O2
{{:chemogenomics:structures:chem-0122.svg?nolink}}
</panel>


<panel title='Compound Supplier' collapsed='true'>
    * **<color #00a2e8>Supplier Name:</color>** Selleck Chemicals
    * **<color #00a2e8>Catalog #:</color>** S1237
    * **<color #00a2e8>Lot #:</color>** N/A
</panel>


<panel title='Compound Characterization' collapsed='true'>
    * **<color #00a2e8>HRMS (ESI-TOF) m/z:</color>** (M+H)+ Calcd for C6H6N6O2 195.0625; found 195.06203
</panel>


<panel title='Dose Response Curve' collapsed='true'>
    * **<color #00a2e8>Platform ID:</color>** Temozolomide
    * **<color #00a2e8>Min:</color>** -5.2800; **<color #00a2e8>Max:</color>** 2.5972
{{:chemogenomics:dose_response:dr_245.png?nolink&500 |}}
\\ 
\\ 
\\ 
\\ 
|< 300px 100px 200px >|
^ IC   ^ Concentration (µM) ^
| IC10  |N/A    |
| IC20  |N/A    |
| IC30  |N/A    |
| IC40  |N/A    |
| IC50  |N/A    |
| IC60  |N/A    |
| IC70  |N/A    |
| IC80  |N/A    |
| IC90  |N/A    |
\\ 
</panel>
</accordion>
==== Screen Summary ====
    * **<color #00a2e8>Round</color>**: 04
    * **<color #00a2e8>Dose</color>**: 200µM
    * **<color #00a2e8>Days of incubation</color>**: 8
    * **<color #00a2e8>Doublings</color>**: 4.9
    * **<color #00a2e8>Numbers of reads</color>**: 18157051


==== Screen Results ====


^Sensitive/Resistant hits (FDR<0.05)^CRANKS^Score Plot^Top 30 Genes^Screen Similarity^Top 30 Sensitive GO terms^Top 30 Resistant GO terms^
|14/19|<color #e85500>[[https://files.tyerslab.com/files/public/chemogenomics/cranks/Temozolomide_200uM_Round-4_exp189.txt|Scores]]</color>|<button type="primary" size="sm" modal="ScorePlot">View</button>|<button type="primary" size="sm" modal="modal_hits">View</button>|<button type="primary" size="sm" modal="modal_screen_similarity">View</button>|<button type="primary" size="sm" modal="modal_go_sensitive">View</button>|<button type="primary" size="sm" modal="modal_go_resistant">View</button>|
<modal id='ScorePlot' size='lg' title='Temozolomide 200μM R04 exp189'>
{{:chemogenomics:cranks_plots:exp189.png?nolink}}
</modal>


<modal id="modal_hits" size="sm" title="Top 30 S/R Genes">
^Gene^CRANKS Score^FDR^
|[[:human_genes:M:MDH1|MDH1]]|-4.26|<0.01|
|[[:human_genes:N:NT5C2|NT5C2]]|-3.69|<0.01|
|[[:human_genes:A:ADIPOR2|ADIPOR2]]|-3.30|<0.01|
|[[:human_genes:M:MCM9|MCM9]]|-3.04|<0.01|
|[[:human_genes:U:UXS1|UXS1]]|-3.03|<0.01|
|[[:human_genes:M:MAD2L2|MAD2L2]]|-2.89|<0.01|
|[[:human_genes:R:RNF25|RNF25]]|-2.89|<0.01|
|[[:human_genes:P:PARP1|PARP1]]|-2.85|<0.01|
|[[:human_genes:O:OTUB1|OTUB1]]|-2.83|0.01|
|[[:human_genes:P:PPCS|PPCS]]|-2.68|0.03|
|[[:human_genes:R:RCC2|RCC2]]|-2.65|0.01|
|[[:human_genes:C:CHD1L|CHD1L]]|-2.59|0.02|
|[[:human_genes:G:GOT1|GOT1]]|-2.56|0.02|
|[[:human_genes:R:RHOA|RHOA]]|-2.44|0.04|
|[[:human_genes:P:PARK2|PARK2]]|-2.37|0.05|
|[[:human_genes:M:MAPK14|MAPK14]]|-2.37|0.05|
|[[:human_genes:A:ACSL4|ACSL4]]|-2.31|0.13|
|[[:human_genes:A:ARL1|ARL1]]|-2.30|0.07|
|[[:human_genes:N:NSFL1C|NSFL1C]]|-2.27|0.08|
|[[:human_genes:R:RPL36A-HNRNPH2|RPL36A-HNRNPH2]]|-2.25|0.62|
|[[:human_genes:M:MYH13|MYH13]]|-2.24|0.24|
|[[:human_genes:U:UBE2D3|UBE2D3]]|-2.19|0.12|
|[[:human_genes:F:FCRLA|FCRLA]]|-2.16|0.13|
|[[:human_genes:P:PRPS1|PRPS1]]|-2.16|0.21|
|[[:human_genes:P:PNKP|PNKP]]|-2.16|0.36|
|[[:human_genes:P:POLB|POLB]]|-2.14|0.14|
|[[:human_genes:S:STIP1|STIP1]]|-2.14|0.21|
|[[:human_genes:S:SNF8|SNF8]]|-2.13|0.21|
|[[:human_genes:A:ATM|ATM]]|-2.11|0.16|
|[[:human_genes:R:RSRC2|RSRC2]]|-2.11|0.40|
|[[:human_genes:T:TP53BP2|TP53BP2]]|2.22|0.08|
|[[:human_genes:S:SEPT4|SEPT4]]|2.24|0.08|
|[[:human_genes:A:AGAP5|AGAP5]]|2.26|0.07|
|[[:human_genes:I:INPP5F|INPP5F]]|2.30|0.06|
|[[:human_genes:T:TSC1|TSC1]]|2.30|0.06|
|[[:human_genes:N:NAA38|NAA38]]|2.31|0.22|
|[[:human_genes:U:U2SURP|U2SURP]]|2.34|0.21|
|[[:human_genes:D:DDX3X|DDX3X]]|2.34|0.05|
|[[:human_genes:C:C2orf43|C2orf43]]|2.36|0.08|
|[[:human_genes:I:IBA57|IBA57]]|2.36|0.05|
|[[:human_genes:C:CNGB3|CNGB3]]|2.43|0.03|
|[[:human_genes:P:PAICS|PAICS]]|2.48|0.35|
|[[:human_genes:T:TSC2|TSC2]]|2.52|0.02|
|[[:human_genes:U:USP16|USP16]]|2.56|0.02|
|[[:human_genes:P:PNO1|PNO1]]|2.58|0.21|
|[[:human_genes:N:NPRL2|NPRL2]]|2.58|0.02|
|[[:human_genes:S:SLC25A1|SLC25A1]]|2.67|0.01|
|[[:human_genes:C:CMAS|CMAS]]|2.79|<0.01|
|[[:human_genes:M:MDH2|MDH2]]|2.86|0.01|
|[[:human_genes:A:ABHD11|ABHD11]]|2.86|<0.01|
|[[:human_genes:S:SFXN4|SFXN4]]|2.89|0.01|
|[[:human_genes:I:IMPDH2|IMPDH2]]|2.92|<0.01|
|[[:human_genes:N:NUDT5|NUDT5]]|2.93|<0.01|
|[[:human_genes:A:ARID4B|ARID4B]]|2.96|<0.01|
|[[:human_genes:N:NPRL3|NPRL3]]|2.97|0.01|
|[[:human_genes:G:GOT2|GOT2]]|2.98|<0.01|
|[[:human_genes:N:NANS|NANS]]|3.52|<0.01|
|[[:human_genes:G:GCN1L1|GCN1L1]]|4.28|<0.01|
|[[:human_genes:H:HPRT1|HPRT1]]|6.54|<0.01|
|[[:human_genes:E:EIF2AK4|EIF2AK4]]|6.69|<0.01|
</modal>


<modal id="modal_screen_similarity" size="lg" title="Correlated Screens">
^Screen^Correlation^Plot^
|[[:results:exp216|Erlotinib 10μM R05 exp216]]|0.098|<button type="primary" size="small" modal="1">View</button>|
</modal>


<modal id="1" size="lg" title="Erlotinib 10μM R05 exp216  vs  Temozolomide 200μM R04 exp189">
{{:chemogenomics:comparison_plots:exp189_vs_exp216.png?nolink |}}
</modal>



<modal id="modal_go_sensitive" size="lg" title="Top 30 sensitive GO terms">
^GO Term^Fold Change^Genes^
|site of double-strand break|30.31|MAD2L2,PARP1,CHD1L,PNKP|
|double-strand break repair|18.32|MCM9,MAD2L2,PARP1,PNKP,POLB,ATM|
|DNA repair|11.75|MCM9,MAD2L2,PARP1,OTUB1,CHD1L,UBE2D3,PNKP,POLB,ATM|
|DNA damage response|8.61|MCM9,MAD2L2,PARP1,OTUB1,CHD1L,MAPK14,UBE2D3,PNKP,POLB,ATM|
|nucleobase-containing small molecule metabolic process|8.05|MDH1,NT5C2,UXS1,PARP1,PPCS,ACSL4,PRPS1|
|DNA metabolic process|7.74|MCM9,MAD2L2,PARP1,OTUB1,CHD1L,UBE2D3,PNKP,POLB,ATM|
</modal>
<modal id="modal_go_resistant" size="lg" title="Top 30 resistant GO terms">
^GO Term^Fold Change^Genes^
|GATOR1 complex|552.47|NPRL3,NPRL2|
|XMP metabolic process|184.16|IMPDH2,PAICS|
|positive regulation of translation in response to stress|184.16|EIF2AK4,DDX3X|
|GMP biosynthetic process|127.49|HPRT1,IMPDH2,PAICS|
|purine ribonucleoside monophosphate biosynthetic process|92.08|HPRT1,IMPDH2,PAICS|
|purine nucleoside monophosphate biosynthetic process|82.87|HPRT1,IMPDH2,PAICS|
|GMP metabolic process|61.39|HPRT1,IMPDH2,PAICS|
|negative regulation of TORC1 signaling|53.90|NPRL3,NPRL2,TSC2,TSC1|
|ribonucleoside monophosphate biosynthetic process|48.75|HPRT1,IMPDH2,PAICS|
|cellular response to amino acid starvation|46.04|EIF2AK4,GCN1,NPRL3,NPRL2|
|response to amino acid starvation|43.33|EIF2AK4,GCN1,NPRL3,NPRL2|
|negative regulation of TOR signaling|33.48|NPRL3,NPRL2,TSC2,TSC1|
|regulation of TORC1 signaling|25.70|NPRL3,NPRL2,TSC2,TSC1|
|cellular response to starvation|19.38|EIF2AK4,GCN1,NPRL3,NPRL2,TSC2,TSC1|
|response to starvation|15.78|EIF2AK4,GCN1,NPRL3,NPRL2,TSC2,TSC1|
|cellular response to nutrient levels|14.23|EIF2AK4,GCN1,NPRL3,NPRL2,TSC2,TSC1|
|cellular response to extracellular stimulus|12.65|EIF2AK4,GCN1,NPRL3,NPRL2,TSC2,TSC1|
|cellular response to external stimulus|10.14|EIF2AK4,GCN1,NPRL3,NPRL2,TSC2,TSC1|
|nucleobase-containing small molecule metabolic process|7.24|HPRT1,NANS,NUDT5,IMPDH2,MDH2,SLC25A1,PAICS|
</modal>