Demecolcine 0.03μM R05 exp228 [The Human Chemical-Genetic Interaction Map Project]

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====== Demecolcine 0.03μM R05 exp228 ======
==== Mechanism of Action ====
Inhibits microtubule polymerization, a.k.a. colcemid
  * **<color #00a2e8>Class / Subclass 1:</color>** Cell Cycle / Microtubule Poison
  * **<color #00a2e8>Class / Subclass 2:</color>** Organelle Function / Cytoskeletal Inhibitor



==== Technical Notes ====
<accordion collapsed='true'>
<panel title='Compound References' collapsed='true'>
    * **<color #00a2e8>PubChem Name:</color>** %%Demecolcine%%
    * **<color #00a2e8>Synonyms:</color>** N/A
    * **<color #00a2e8>CAS #:</color>** 477-30-5
    * **<color #00a2e8>PubChem CID:</color>** [[https://pubchem.ncbi.nlm.nih.gov/compound/220401|220401]]
    * **<color #00a2e8>IUPAC:</color>** %%(7S)-1,2,3,10-tetramethoxy-7-(methylamino)-6,7-dihydro-5H-benzo[a]heptalen-9-one%%
    * **<color #00a2e8>INCHI Name:</color>** InChI=1S/C21H25NO5/c1-22-15-8-6-12-10-18(25-3)20(26-4)21(27-5)19(12)13-7-9-17(24-2)16(23)11-14(13)15/h7,9-11,15,22H,6,8H2,1-5H3/t15-/m0/s1
    * **<color #00a2e8>INCHI Key:</color>** NNJPGOLRFBJNIW-HNNXBMFYSA-N
    * **<color #00a2e8>Molecular Weight:</color>** 371.4
    * **<color #00a2e8>Canonical SMILES:</color>** CNC1CCC2=CC(=C(C(=C2C3=CC=C(C(=O)C=C13)OC)OC)OC)OC
    * **<color #00a2e8>Isomeric SMILES:</color>** CN[C@H]1CCC2=CC(=C(C(=C2C3=CC=C(C(=O)C=C13)OC)OC)OC)OC
    * **<color #00a2e8>Molecular Formula:</color>** C21H25NO5
{{:chemogenomics:structures:chem-0133.svg?nolink}}
</panel>


<panel title='Compound Supplier' collapsed='true'>
    * **<color #00a2e8>Supplier Name:</color>** Sigma-Aldrich
    * **<color #00a2e8>Catalog #:</color>** D7385
    * **<color #00a2e8>Lot #:</color>** N/A
</panel>


<panel title='Compound Characterization' collapsed='true'>
    * **<color #00a2e8>HRMS (ESI-TOF) m/z:</color>** (M+H)+ Calcd for C21H25NO5 372.18055; found 372.18123
</panel>


<panel title='Dose Response Curve' collapsed='true'>
    * **<color #00a2e8>Platform ID:</color>** Demecolcine
    * **<color #00a2e8>Min:</color>** -11.2124; **<color #00a2e8>Max:</color>** 88.4000
{{:chemogenomics:dose_response:dr_207.png?nolink&500 |}}
\\ 
\\ 
\\ 
\\ 
|< 300px 100px 200px >|
^ IC   ^ Concentration (µM) ^
| IC10  |N/A    |
| IC20  |0.0336    |
| IC30  |0.0347    |
| IC40  |0.0356    |
| IC50  |0.0365    |
| IC60  |N/A    |
| IC70  |N/A    |
| IC80  |N/A    |
| IC90  |N/A    |
\\ 
</panel>
</accordion>
==== Screen Summary ====
    * **<color #00a2e8>Round</color>**: 05
    * **<color #00a2e8>Dose</color>**: 30nM
    * **<color #00a2e8>Days of incubation</color>**: 8
    * **<color #00a2e8>Doublings</color>**: 5.8
    * **<color #00a2e8>Numbers of reads</color>**: 23433703


==== Screen Results ====


^Sensitive/Resistant hits (FDR<0.05)^CRANKS^Score Plot^Top 30 Genes^Screen Similarity^Top 30 Sensitive GO terms^Top 30 Resistant GO terms^
|45/9|<color #e85500>[[https://files.tyerslab.com/files/public/chemogenomics/cranks/Demecolcine_0.03uM_Round-5_exp228.txt|Scores]]</color>|<button type="primary" size="sm" modal="ScorePlot">View</button>|<button type="primary" size="sm" modal="modal_hits">View</button>|<button type="primary" size="sm" modal="modal_screen_similarity">View</button>|<button type="primary" size="sm" modal="modal_go_sensitive">View</button>|<button type="primary" size="sm" modal="modal_go_resistant">View</button>|
<modal id='ScorePlot' size='lg' title='Demecolcine 0.03μM R05 exp228'>
{{:chemogenomics:cranks_plots:exp228.png?nolink}}
</modal>


<modal id="modal_hits" size="sm" title="Top 30 S/R Genes">
^Gene^CRANKS Score^FDR^
|[[:human_genes:K:KIF15|KIF15]]|-5.67|<0.01|
|[[:human_genes:S:STMN1|STMN1]]|-4.27|<0.01|
|[[:human_genes:L:LRRC8A|LRRC8A]]|-3.61|<0.01|
|[[:human_genes:T:TCF4|TCF4]]|-3.58|<0.01|
|[[:human_genes:A:ASB3|ASB3]]|-3.58|<0.01|
|[[:human_genes:T:TACC3|TACC3]]|-3.48|<0.01|
|[[:human_genes:A:ASPM|ASPM]]|-3.42|<0.01|
|[[:human_genes:B:BRD1|BRD1]]|-3.41|<0.01|
|[[:human_genes:D:DLGAP5|DLGAP5]]|-3.39|<0.01|
|[[:human_genes:P:PDS5B|PDS5B]]|-3.27|<0.01|
|[[:human_genes:D:DPH1|DPH1]]|-3.25|<0.01|
|[[:human_genes:S:SPAG5|SPAG5]]|-3.13|<0.01|
|[[:human_genes:T:TUBB4B|TUBB4B]]|-3.02|<0.01|
|[[:human_genes:Q:QRICH1|QRICH1]]|-3.02|<0.01|
|[[:human_genes:S:SEPHS1|SEPHS1]]|-3.01|<0.01|
|[[:human_genes:N:NSL1|NSL1]]|-2.91|<0.01|
|[[:human_genes:N:NPRL2|NPRL2]]|-2.91|<0.01|
|[[:human_genes:D:DSN1|DSN1]]|-2.85|<0.01|
|[[:human_genes:S:SKA1|SKA1]]|-2.74|<0.01|
|[[:human_genes:V:VEZT|VEZT]]|-2.73|<0.01|
|[[:human_genes:P:PSMA3|PSMA3]]|-2.73|0.02|
|[[:human_genes:V:VBP1|VBP1]]|-2.73|<0.01|
|[[:human_genes:P:PFDN5|PFDN5]]|-2.73|<0.01|
|[[:human_genes:M:MAPRE1|MAPRE1]]|-2.72|0.01|
|[[:human_genes:M:MSI2|MSI2]]|-2.70|<0.01|
|[[:human_genes:D:DPH2|DPH2]]|-2.69|<0.01|
|[[:human_genes:T:TBCD|TBCD]]|-2.64|0.01|
|[[:human_genes:Z:ZCRB1|ZCRB1]]|-2.63|0.01|
|[[:human_genes:P:PDCD5|PDCD5]]|-2.63|0.01|
|[[:human_genes:G:GTSE1|GTSE1]]|-2.62|0.01|
|[[:human_genes:S:SYNE3|SYNE3]]|2.17|0.13|
|[[:human_genes:N:NOP16|NOP16]]|2.18|0.36|
|[[:human_genes:T:TUFM|TUFM]]|2.20|0.28|
|[[:human_genes:T:TMEM232|TMEM232]]|2.20|0.17|
|[[:human_genes:P:PDIK1L|PDIK1L]]|2.20|0.13|
|[[:human_genes:C:CLCN6|CLCN6]]|2.24|0.11|
|[[:human_genes:T:TTK|TTK]]|2.29|0.25|
|[[:human_genes:H:HSPE1|HSPE1]]|2.31|0.54|
|[[:human_genes:I:ID4|ID4]]|2.33|0.07|
|[[:human_genes:G:GCH1|GCH1]]|2.34|0.07|
|[[:human_genes:C:CELF1|CELF1]]|2.37|0.06|
|[[:human_genes:T:TMEM161B|TMEM161B]]|2.42|0.06|
|[[:human_genes:P:PAFAH1B1|PAFAH1B1]]|2.43|0.35|
|[[:human_genes:K:KDM5C|KDM5C]]|2.51|0.04|
|[[:human_genes:H:HK2|HK2]]|2.54|0.17|
|[[:human_genes:H:HSP90AB1|HSP90AB1]]|2.57|0.06|
|[[:human_genes:P:PGP|PGP]]|2.58|0.06|
|[[:human_genes:S:SCAF1|SCAF1]]|2.66|0.40|
|[[:human_genes:N:NUMA1|NUMA1]]|2.68|0.06|
|[[:human_genes:C:C15orf38-AP3S2|C15orf38-AP3S2]]|2.78|0.03|
|[[:human_genes:Y:YWHAQ|YWHAQ]]|2.89|0.02|
|[[:human_genes:R:RALGAPB|RALGAPB]]|2.89|<0.01|
|[[:human_genes:L:LIG1|LIG1]]|2.90|0.31|
|[[:human_genes:D:DYNC1H1|DYNC1H1]]|2.90|0.31|
|[[:human_genes:H:HMGCR|HMGCR]]|2.95|0.19|
|[[:human_genes:A:ADIPOR1|ADIPOR1]]|2.97|<0.01|
|[[:human_genes:A:ATP1A1|ATP1A1]]|3.05|<0.01|
|[[:human_genes:S:SAMHD1|SAMHD1]]|3.09|<0.01|
|[[:human_genes:S:SCAPER|SCAPER]]|3.60|<0.01|
|[[:human_genes:A:ATAT1|ATAT1]]|4.10|<0.01|
</modal>


<modal id="modal_screen_similarity" size="lg" title="Correlated Screens">
^Screen^Correlation^Plot^
|[[:results:exp217|Mdivi-1 15μM R05 exp217]]|0.12|<button type="primary" size="small" modal="1">View</button>|
|[[:results:exp190|Vincristine 0.0005μM R04 exp190]]|0.113|<button type="primary" size="small" modal="2">View</button>|
|[[:results:exp60|Vinblastine 0.002μM R01 exp60]]|0.09|<button type="primary" size="small" modal="3">View</button>|
|[[:results:exp243|S-trityl-L-cysteine 0.5μM R05 exp243]]|0.083|<button type="primary" size="small" modal="4">View</button>|
|[[:results:exp517|Quercetin 20μM R08 exp517]]|0.082|<button type="primary" size="small" modal="5">View</button>|
|[[:results:exp59|UMK57 1μM R01 exp59]]|0.081|<button type="primary" size="small" modal="6">View</button>|
|[[:results:exp184|Ixabepilone 0.004 to 0.005μM on day4 R04 exp184]]|0.075|<button type="primary" size="small" modal="7">View</button>|
|[[:results:exp106|UM131593 0.2μM R03 exp106]]|0.068|<button type="primary" size="small" modal="8">View</button>|
|[[:results:exp103|Taxol 0.004μM R03 exp103]]|0.066|<button type="primary" size="small" modal="9">View</button>|
|[[:results:exp526|Sulforaphane 9μM R08 exp526 no dilution day6]]|0.06|<button type="primary" size="small" modal="10">View</button>|
|[[:results:exp125|GSK461364A 0.005μM R03 exp125]]|0.058|<button type="primary" size="small" modal="11">View</button>|
|[[:results:exp107|UMK57 0.6μM R03 exp107]]|0.055|<button type="primary" size="small" modal="12">View</button>|
|[[:results:exp485|GSK626616 14μM R08 exp485]]|0.055|<button type="primary" size="small" modal="13">View</button>|
|[[:results:exp525|Sulforaphane 9μM R08 exp525]]|0.052|<button type="primary" size="small" modal="14">View</button>|
|[[:results:exp231|Epothilone-B 0.0015μM R05 exp231]]|0.052|<button type="primary" size="small" modal="15">View</button>|
|[[:results:exp215|Colchicine 0.009μM R05 exp215]]|0.189|<button type="primary" size="small" modal="16">View</button>|
|[[:results:exp179|Combretastatin A4 0.002 to 0.003μM day4 R04 exp179]]|0.182|<button type="primary" size="small" modal="17">View</button>|
|[[:results:exp312|2-Methoxyestradiol 0.55 to 1μM on day4 R07 exp312]]|0.123|<button type="primary" size="small" modal="18">View</button>|
|[[:results:exp460|BML-284 0.09μM R08 exp460]]|0.121|<button type="primary" size="small" modal="19">View</button>|
|[[:results:exp311|2-Methoxyestradiol 0.55 to 0.75μM  on day4 R07 exp311]]|0.095|<button type="primary" size="small" modal="20">View</button>|
|[[:results:exp310|2-Methoxyestradiol 0.55μM R07 exp310]]|0.067|<button type="primary" size="small" modal="21">View</button>|
|[[:results:exp97|BI-6727 0.0125μM R03 exp97]]|0.058|<button type="primary" size="small" modal="22">View</button>|
|[[:results:exp95|BI-2536 0.0042μM R03 exp95]]|0.056|<button type="primary" size="small" modal="23">View</button>|
</modal>


<modal id="1" size="lg" title="Demecolcine 0.03μM R05 exp228  vs  Mdivi-1 15μM R05 exp217">
{{:chemogenomics:comparison_plots:exp217_vs_exp228.png?nolink |}}
</modal>

<modal id="2" size="lg" title="Demecolcine 0.03μM R05 exp228  vs  Vincristine 0.0005μM R04 exp190">
{{:chemogenomics:comparison_plots:exp190_vs_exp228.png?nolink |}}
</modal>

<modal id="3" size="lg" title="Demecolcine 0.03μM R05 exp228  vs  Vinblastine 0.002μM R01 exp60">
{{:chemogenomics:comparison_plots:exp228_vs_exp60.png?nolink |}}
</modal>

<modal id="4" size="lg" title="Demecolcine 0.03μM R05 exp228  vs  S-trityl-L-cysteine 0.5μM R05 exp243">
{{:chemogenomics:comparison_plots:exp228_vs_exp243.png?nolink |}}
</modal>

<modal id="5" size="lg" title="Demecolcine 0.03μM R05 exp228  vs  Quercetin 20μM R08 exp517">
{{:chemogenomics:comparison_plots:exp228_vs_exp517.png?nolink |}}
</modal>

<modal id="6" size="lg" title="Demecolcine 0.03μM R05 exp228  vs  UMK57 1μM R01 exp59">
{{:chemogenomics:comparison_plots:exp228_vs_exp59.png?nolink |}}
</modal>

<modal id="7" size="lg" title="Demecolcine 0.03μM R05 exp228  vs  Ixabepilone 0.004 to 0.005μM on day4 R04 exp184">
{{:chemogenomics:comparison_plots:exp184_vs_exp228.png?nolink |}}
</modal>

<modal id="8" size="lg" title="Demecolcine 0.03μM R05 exp228  vs  UM131593 0.2μM R03 exp106">
{{:chemogenomics:comparison_plots:exp106_vs_exp228.png?nolink |}}
</modal>

<modal id="9" size="lg" title="Demecolcine 0.03μM R05 exp228  vs  Taxol 0.004μM R03 exp103">
{{:chemogenomics:comparison_plots:exp103_vs_exp228.png?nolink |}}
</modal>

<modal id="10" size="lg" title="Demecolcine 0.03μM R05 exp228  vs  Sulforaphane 9μM R08 exp526 no dilution day6">
{{:chemogenomics:comparison_plots:exp228_vs_exp526.png?nolink |}}
</modal>

<modal id="11" size="lg" title="Demecolcine 0.03μM R05 exp228  vs  GSK461364A 0.005μM R03 exp125">
{{:chemogenomics:comparison_plots:exp125_vs_exp228.png?nolink |}}
</modal>

<modal id="12" size="lg" title="Demecolcine 0.03μM R05 exp228  vs  UMK57 0.6μM R03 exp107">
{{:chemogenomics:comparison_plots:exp107_vs_exp228.png?nolink |}}
</modal>

<modal id="13" size="lg" title="Demecolcine 0.03μM R05 exp228  vs  GSK626616 14μM R08 exp485">
{{:chemogenomics:comparison_plots:exp228_vs_exp485.png?nolink |}}
</modal>

<modal id="14" size="lg" title="Demecolcine 0.03μM R05 exp228  vs  Sulforaphane 9μM R08 exp525">
{{:chemogenomics:comparison_plots:exp228_vs_exp525.png?nolink |}}
</modal>

<modal id="15" size="lg" title="Demecolcine 0.03μM R05 exp228  vs  Epothilone-B 0.0015μM R05 exp231">
{{:chemogenomics:comparison_plots:exp228_vs_exp231.png?nolink |}}
</modal>

<modal id="16" size="lg" title="Colchicine 0.009μM R05 exp215  vs  Demecolcine 0.03μM R05 exp228">
{{:chemogenomics:comparison_plots:exp215_vs_exp228.png?nolink |}}
</modal>

<modal id="17" size="lg" title="Combretastatin A4 0.002 to 0.003μM day4 R04 exp179  vs  Demecolcine 0.03μM R05 exp228">
{{:chemogenomics:comparison_plots:exp179_vs_exp228.png?nolink |}}
</modal>

<modal id="18" size="lg" title="2-Methoxyestradiol 0.55 to 1μM on day4 R07 exp312  vs  Demecolcine 0.03μM R05 exp228">
{{:chemogenomics:comparison_plots:exp228_vs_exp312.png?nolink |}}
</modal>

<modal id="19" size="lg" title="BML-284 0.09μM R08 exp460  vs  Demecolcine 0.03μM R05 exp228">
{{:chemogenomics:comparison_plots:exp228_vs_exp460.png?nolink |}}
</modal>

<modal id="20" size="lg" title="2-Methoxyestradiol 0.55 to 0.75μM  on day4 R07 exp311  vs  Demecolcine 0.03μM R05 exp228">
{{:chemogenomics:comparison_plots:exp228_vs_exp311.png?nolink |}}
</modal>

<modal id="21" size="lg" title="2-Methoxyestradiol 0.55μM R07 exp310  vs  Demecolcine 0.03μM R05 exp228">
{{:chemogenomics:comparison_plots:exp228_vs_exp310.png?nolink |}}
</modal>

<modal id="22" size="lg" title="BI-6727 0.0125μM R03 exp97  vs  Demecolcine 0.03μM R05 exp228">
{{:chemogenomics:comparison_plots:exp228_vs_exp97.png?nolink |}}
</modal>

<modal id="23" size="lg" title="BI-2536 0.0042μM R03 exp95  vs  Demecolcine 0.03μM R05 exp228">
{{:chemogenomics:comparison_plots:exp228_vs_exp95.png?nolink |}}
</modal>



<modal id="modal_go_sensitive" size="lg" title="Top 30 sensitive GO terms">
^GO Term^Fold Change^Genes^
|negative regulation of microtubule polymerization|142.84|STMN1,MAPRE1,TBCD|
|outer kinetochore|90.22|NSL1,DSN1,SKA1|
|spindle pole|23.53|TACC3,ASPM,DLGAP5,SPAG5,NSL1,DSN1,MAPRE1|
|mitotic spindle|22.60|TACC3,ASPM,DLGAP5,SPAG5,TUBB4B,SKA1,MAPRE1|
|regulation of microtubule cytoskeleton organization|21.56|STMN1,TACC3,SPAG5,SKA1,MAPRE1,TBCD|
|mitotic sister chromatid segregation|21.01|DLGAP5,SPAG5,NSL1,DSN1,MAPRE1|
|sister chromatid segregation|20.41|DLGAP5,SPAG5,NSL1,DSN1,MAPRE1|
|spindle organization|19.93|STMN1,TACC3,ASPM,DLGAP5,SPAG5,MAPRE1|
|tubulin binding|14.05|KIF15,STMN1,DLGAP5,SPAG5,SKA1,VBP1,MAPRE1,TBCD,PDCD5|
|regulation of microtubule-based process|13.99|STMN1,TACC3,SPAG5,SKA1,MAPRE1,TBCD|
|spindle|13.80|KIF15,TACC3,ASPM,DLGAP5,SPAG5,TUBB4B,NSL1,DSN1,SKA1,MAPRE1|
|chromosome segregation|12.23|ASPM,DLGAP5,SPAG5,NSL1,DSN1,SKA1,MAPRE1|
|microtubule|10.53|KIF15,STMN1,ASPM,SPAG5,TUBB4B,SKA1,MAPRE1,TBCD|
|mitotic cell cycle|10.44|KIF15,STMN1,TACC3,DLGAP5,SPAG5,TUBB4B,NSL1,DSN1,SKA1,MAPRE1,TBCD|
|cell division|10.16|STMN1,TACC3,ASPM,PDS5B,SPAG5,NSL1,DSN1,SKA1,MAPRE1|
|microtubule cytoskeleton organization|8.48|STMN1,TACC3,ASPM,DLGAP5,SPAG5,TUBB4B,MAPRE1,TBCD|
|microtubule-based process|7.33|KIF15,STMN1,TACC3,ASPM,DLGAP5,SPAG5,TUBB4B,VBP1,MAPRE1,TBCD|
|cell cycle process|6.54|STMN1,TACC3,ASPM,DLGAP5,PDS5B,SPAG5,NSL1,DSN1,SKA1,MAPRE1|
|cytoskeletal protein binding|5.88|KIF15,STMN1,DLGAP5,SPAG5,SKA1,VEZT,VBP1,MAPRE1,TBCD,PDCD5|
</modal>
<modal id="modal_go_resistant" size="lg" title="Top 30 resistant GO terms">
^GO Term^Fold Change^Genes^
|dynein complex binding|72.04|NUMA1,PAFAH1B1,KATNB1|
</modal>