Epigallocatechin gallate 20μM R05 exp230 [The Human Chemical-Genetic Interaction Map Project]

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====== Epigallocatechin gallate 20μM R05 exp230 ======
==== Mechanism of Action ====
Polyphenol antioxidant from green tea, numerous claimed activities
  * **<color #00a2e8>Class / Subclass 1:</color>** Metabolism / Nutraceutical
  * **<color #00a2e8>Class / Subclass 2:</color>** Environmental Stresses / Food Additive



==== Technical Notes ====
<accordion collapsed='true'>
<panel title='Compound References' collapsed='true'>
    * **<color #00a2e8>PubChem Name:</color>** %%(-)-Epigallocatechin gallate%%
    * **<color #00a2e8>Synonyms:</color>** EGCG; Epigallocatechol Gallate; EGCG;Epigallocatechol Gallate
    * **<color #00a2e8>CAS #:</color>** 989-51-5
    * **<color #00a2e8>PubChem CID:</color>** [[https://pubchem.ncbi.nlm.nih.gov/compound/65064|65064]]
    * **<color #00a2e8>IUPAC:</color>** %%[(2R,3R)-5,7-dihydroxy-2-(3,4,5-trihydroxyphenyl)-3,4-dihydro-2H-chromen-3-yl] 3,4,5-trihydroxybenzoate%%
    * **<color #00a2e8>INCHI Name:</color>** InChI=1S/C22H18O11/c23-10-5-12(24)11-7-18(33-22(31)9-3-15(27)20(30)16(28)4-9)21(32-17(11)6-10)8-1-13(25)19(29)14(26)2-8/h1-6,18,21,23-30H,7H2/t18-,21-/m1/s1
    * **<color #00a2e8>INCHI Key:</color>** WMBWREPUVVBILR-WIYYLYMNSA-N
    * **<color #00a2e8>Molecular Weight:</color>** 458.4
    * **<color #00a2e8>Canonical SMILES:</color>** C1C(C(OC2=CC(=CC(=C21)O)O)C3=CC(=C(C(=C3)O)O)O)OC(=O)C4=CC(=C(C(=C4)O)O)O
    * **<color #00a2e8>Isomeric SMILES:</color>** C1[C@H]([C@H](OC2=CC(=CC(=C21)O)O)C3=CC(=C(C(=C3)O)O)O)OC(=O)C4=CC(=C(C(=C4)O)O)O
    * **<color #00a2e8>Molecular Formula:</color>** C22H18O11
{{:chemogenomics:structures:chem-0135.svg?nolink}}
</panel>


<panel title='Compound Supplier' collapsed='true'>
    * **<color #00a2e8>Supplier Name:</color>** Toronto Research Chemicals
    * **<color #00a2e8>Catalog #:</color>** E588500
    * **<color #00a2e8>Lot #:</color>** N/A
</panel>


<panel title='Compound Characterization' collapsed='true'>
    * **<color #00a2e8>HRMS (ESI-TOF) m/z:</color>** (M+H)+ Calcd for C22H18O11 459.09219; found 459.09204
</panel>


<panel title='Dose Response Curve' collapsed='true'>
    * **<color #00a2e8>Platform ID:</color>** Epigallocatechin gallate
    * **<color #00a2e8>Min:</color>** -8.6600; **<color #00a2e8>Max:</color>** 64.3339
{{:chemogenomics:dose_response:dr_80.png?nolink&500 |}}
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|< 300px 100px 200px >|
^ IC   ^ Concentration (µM) ^
| IC10  |N/A    |
| IC20  |16.9406    |
| IC30  |17.7734    |
| IC40  |18.4865    |
| IC50  |19.1661    |
| IC60  |N/A    |
| IC70  |N/A    |
| IC80  |N/A    |
| IC90  |N/A    |
\\ 
</panel>
</accordion>
==== Screen Summary ====
    * **<color #00a2e8>Round</color>**: 05
    * **<color #00a2e8>Dose</color>**: 20µM
    * **<color #00a2e8>Days of incubation</color>**: 8
    * **<color #00a2e8>Doublings</color>**: 6.0
    * **<color #00a2e8>Numbers of reads</color>**: 16967397


==== Screen Results ====


^Sensitive/Resistant hits (FDR<0.05)^CRANKS^Score Plot^Top 30 Genes^Screen Similarity^Top 30 Sensitive GO terms^Top 30 Resistant GO terms^
|8/3|<color #e85500>[[https://files.tyerslab.com/files/public/chemogenomics/cranks/Epigallocatechin_gallate_20uM_Round-5_exp230.txt|Scores]]</color>|<button type="primary" size="sm" modal="ScorePlot">View</button>|<button type="primary" size="sm" modal="modal_hits">View</button>|<button type="primary" size="sm" modal="modal_screen_similarity">View</button>|<button type="primary" size="sm" modal="modal_go_sensitive">View</button>|<button type="default" size="sm" modal="modal_go_resistant">View</button>|
<modal id='ScorePlot' size='lg' title='Epigallocatechin gallate 20μM R05 exp230'>
{{:chemogenomics:cranks_plots:exp230.png?nolink}}
</modal>


<modal id="modal_hits" size="sm" title="Top 30 S/R Genes">
^Gene^CRANKS Score^FDR^
|[[:human_genes:P:PRDX1|PRDX1]]|-4.74|<0.01|
|[[:human_genes:G:GCLM|GCLM]]|-3.95|<0.01|
|[[:human_genes:S:SLC7A5|SLC7A5]]|-3.55|<0.01|
|[[:human_genes:P:PRDX6|PRDX6]]|-3.18|<0.01|
|[[:human_genes:S:SEPHS2|SEPHS2]]|-2.79|0.01|
|[[:human_genes:S:SEPHS1|SEPHS1]]|-2.76|0.03|
|[[:human_genes:M:MSI2|MSI2]]|-2.61|0.03|
|[[:human_genes:G:GCLC|GCLC]]|-2.59|0.06|
|[[:human_genes:L:LRP8|LRP8]]|-2.56|0.03|
|[[:human_genes:A:ABCA7|ABCA7]]|-2.44|0.06|
|[[:human_genes:H:HOXA9|HOXA9]]|-2.42|0.06|
|[[:human_genes:P:PHPT1|PHPT1]]|-2.40|0.06|
|[[:human_genes:N:NPRL3|NPRL3]]|-2.39|0.20|
|[[:human_genes:P:PPP1R1B|PPP1R1B]]|-2.37|0.06|
|[[:human_genes:C:CAT|CAT]]|-2.36|0.06|
|[[:human_genes:K:KRTAP10-3|KRTAP10-3]]|-2.28|0.21|
|[[:human_genes:S:SLC3A2|SLC3A2]]|-2.26|0.19|
|[[:human_genes:U:USP27X|USP27X]]|-2.23|0.65|
|[[:human_genes:T:TSC2|TSC2]]|-2.23|0.12|
|[[:human_genes:G:GBF1|GBF1]]|-2.18|0.21|
|[[:human_genes:M:METTL14|METTL14]]|-2.15|0.28|
|[[:human_genes:F:FOXD2|FOXD2]]|-2.15|0.18|
|[[:human_genes:O:OSGIN2|OSGIN2]]|-2.13|0.21|
|[[:human_genes:D:DPAGT1|DPAGT1]]|-2.12|0.21|
|[[:human_genes:H:HMX1|HMX1]]|-2.11|0.21|
|[[:human_genes:A:APEX2|APEX2]]|-2.07|0.21|
|[[:human_genes:Z:ZC3HC1|ZC3HC1]]|-2.07|0.21|
|[[:human_genes:C:CNOT2|CNOT2]]|-2.05|0.21|
|[[:human_genes:G:GGPS1|GGPS1]]|-2.05|0.21|
|[[:human_genes:C:C10orf88|C10orf88]]|-2.05|0.21|
|[[:human_genes:M:MRGPRE|MRGPRE]]|2.04|0.30|
|[[:human_genes:T:TBC1D29|TBC1D29]]|2.04|0.30|
|[[:human_genes:P:PEX6|PEX6]]|2.07|0.30|
|[[:human_genes:B:BTN2A1|BTN2A1]]|2.08|0.33|
|[[:human_genes:R:RPS15A|RPS15A]]|2.09|0.69|
|[[:human_genes:M:MORC2|MORC2]]|2.10|0.28|
|[[:human_genes:M:MAT2A|MAT2A]]|2.12|0.58|
|[[:human_genes:C:CHST8|CHST8]]|2.13|0.32|
|[[:human_genes:P:PRELID1|PRELID1]]|2.16|0.30|
|[[:human_genes:T:TRIT1|TRIT1]]|2.17|0.23|
|[[:human_genes:D:DNMBP|DNMBP]]|2.18|0.23|
|[[:human_genes:C:CKAP5|CKAP5]]|2.19|0.45|
|[[:human_genes:C:COPB2|COPB2]]|2.20|0.45|
|[[:human_genes:N:NBPF12|NBPF12]]|2.21|0.64|
|[[:human_genes:H:HMGN5|HMGN5]]|2.38|0.28|
|[[:human_genes:R:RETSAT|RETSAT]]|2.39|0.13|
|[[:human_genes:A:AMHR2|AMHR2]]|2.39|0.22|
|[[:human_genes:C:CYB561A3|CYB561A3]]|2.41|0.22|
|[[:human_genes:T:TMED2|TMED2]]|2.42|0.13|
|[[:human_genes:F:FAM194B|FAM194B]]|2.48|0.23|
|[[:human_genes:N:NPIPA5|NPIPA5]]|2.48|0.40|
|[[:human_genes:P:PLVAP|PLVAP]]|2.60|0.06|
|[[:human_genes:M:MRPL41|MRPL41]]|2.61|0.33|
|[[:human_genes:U:USP17L17|USP17L17]]|2.61|0.47|
|[[:human_genes:D:DYNC1H1|DYNC1H1]]|2.64|0.47|
|[[:human_genes:A:ANKH|ANKH]]|2.68|0.04|
|[[:human_genes:I:IREB2|IREB2]]|2.76|0.04|
|[[:human_genes:P:PEX12|PEX12]]|2.77|0.04|
|[[:human_genes:P:POLA1|POLA1]]|3.22|0.23|
|[[:human_genes:D:DDX46|DDX46]]|3.53|0.28|
</modal>


<modal id="modal_screen_similarity" size="lg" title="Correlated Screens">
^Screen^Correlation^Plot^
|[[:results:exp133|MKC9989 10μM R03 exp133]]|0.059|<button type="primary" size="small" modal="1">View</button>|
|[[:results:exp292|Menadione 5μM R06 exp292]]|0.053|<button type="primary" size="small" modal="2">View</button>|
|[[:results:exp356|Docosahexaenoic-acid 50μM R07 exp356]]|0.088|<button type="primary" size="small" modal="3">View</button>|
|[[:results:exp162|BI-D1870 2μM R04 exp162]]|0.062|<button type="primary" size="small" modal="4">View</button>|
</modal>


<modal id="1" size="lg" title="Epigallocatechin gallate 20μM R05 exp230  vs  MKC9989 10μM R03 exp133">
{{:chemogenomics:comparison_plots:exp133_vs_exp230.png?nolink |}}
</modal>

<modal id="2" size="lg" title="Epigallocatechin gallate 20μM R05 exp230  vs  Menadione 5μM R06 exp292">
{{:chemogenomics:comparison_plots:exp230_vs_exp292.png?nolink |}}
</modal>

<modal id="3" size="lg" title="Docosahexaenoic-acid 50μM R07 exp356  vs  Epigallocatechin gallate 20μM R05 exp230">
{{:chemogenomics:comparison_plots:exp230_vs_exp356.png?nolink |}}
</modal>

<modal id="4" size="lg" title="BI-D1870 2μM R04 exp162  vs  Epigallocatechin gallate 20μM R05 exp230">
{{:chemogenomics:comparison_plots:exp162_vs_exp230.png?nolink |}}
</modal>



<modal id="modal_go_sensitive" size="lg" title="Top 30 sensitive GO terms">
^GO Term^Fold Change^Genes^
|leucine import across plasma membrane|571.38|SLC7A5,SLC3A2|
|phenylalanine transport|380.92|SLC7A5,SLC3A2|
|valine transport|380.92|SLC7A5,SLC3A2|
|L-histidine transport|380.92|SLC7A5,SLC3A2|
|histidine transport|380.92|SLC7A5,SLC3A2|
|response to human chorionic gonadotropin|285.69|GCLM,GCLC|
|isoleucine transport|285.69|SLC7A5,SLC3A2|
|L-leucine transmembrane transporter activity|228.55|SLC7A5,SLC3A2|
</modal>
<modal id="modal_go_resistant" size="lg" title="Top 30 resistant GO terms">No GO term hits below threshold. (FDR <0.05)</modal>