Geldanamycin 0.01μM R05 exp235 [The Human Chemical-Genetic Interaction Map Project]

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====== Geldanamycin 0.01μM R05 exp235 ======
==== Mechanism of Action ====
HSP90 inhibitor
  * **<color #00a2e8>Class / Subclass 1:</color>** Proteostasis / Chaperone Inhibitor




==== Technical Notes ====
<accordion collapsed='true'>
<panel title='Compound References' collapsed='true'>
    * **<color #00a2e8>PubChem Name:</color>** %%Geldanamycin%%
    * **<color #00a2e8>Synonyms:</color>** N/A
    * **<color #00a2e8>CAS #:</color>** 30562-34-6
    * **<color #00a2e8>PubChem CID:</color>** [[https://pubchem.ncbi.nlm.nih.gov/compound/5288382|5288382]]
    * **<color #00a2e8>IUPAC:</color>** %%[(4E,6Z,8S,9S,10E,12S,13R,14S,16R)-13-hydroxy-8,14,19-trimethoxy-4,10,12,16-tetramethyl-3,20,22-trioxo-2-azabicyclo[16.3.1]docosa-1(21),4,6,10,18-pentaen-9-yl] carbamate%%
    * **<color #00a2e8>INCHI Name:</color>** InChI=1S/C29H40N2O9/c1-15-11-19-25(34)20(14-21(32)27(19)39-7)31-28(35)16(2)9-8-10-22(37-5)26(40-29(30)36)18(4)13-17(3)24(33)23(12-15)38-6/h8-10,13-15,17,22-24,26,33H,11-12H2,1-7H3,(H2,30,36)(H,31,35)/b10-8-,16-9+,18-13+/t15-,17+,22+,23+,24-,26+/m1/s1
    * **<color #00a2e8>INCHI Key:</color>** QTQAWLPCGQOSGP-KSRBKZBZSA-N
    * **<color #00a2e8>Molecular Weight:</color>** 560.6
    * **<color #00a2e8>Canonical SMILES:</color>** CC1CC(C(C(C=C(C(C(C=CC=C(C(=O)NC2=CC(=O)C(=C(C1)C2=O)OC)C)OC)OC(=O)N)C)C)O)OC
    * **<color #00a2e8>Isomeric SMILES:</color>** C[C@H]1C[C@@H]([C@@H]([C@H](/C=C(/[C@@H]([C@H](/C=C\\C=C(\\C(=O)NC2=CC(=O)C(=C(C1)C2=O)OC)/C)OC)OC(=O)N)\\C)C)O)OC
    * **<color #00a2e8>Molecular Formula:</color>** C29H40N2O9
{{:chemogenomics:structures:chem-0140.svg?nolink}}
</panel>


<panel title='Compound Supplier' collapsed='true'>
    * **<color #00a2e8>Supplier Name:</color>** Ark Pharm Inc.
    * **<color #00a2e8>Catalog #:</color>** AK130527
    * **<color #00a2e8>Lot #:</color>** WG0131921-180318001
</panel>


<panel title='Compound Characterization' collapsed='true'>
    * **<color #00a2e8>HRMS (ESI-TOF) m/z:</color>** (M+Na)+ Calcd for C29 H40 N2 O9 583.2626; found 583.26208
</panel>


<panel title='Dose Response Curve' collapsed='true'>
    * **<color #00a2e8>Platform ID:</color>** Gelndanamycin
    * **<color #00a2e8>Min:</color>** 7.6058; **<color #00a2e8>Max:</color>** 99.7682
{{:chemogenomics:dose_response:dr_266.png?nolink&500 |}}
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|< 300px 100px 200px >|
^ IC   ^ Concentration (µM) ^
| IC10  |N/A    |
| IC20  |0.0045    |
| IC30  |0.0122    |
| IC40  |0.0275    |
| IC50  |0.0582    |
| IC60  |N/A    |
| IC70  |N/A    |
| IC80  |N/A    |
| IC90  |N/A    |
\\ 
</panel>
</accordion>
==== Screen Summary ====
    * **<color #00a2e8>Round</color>**: 05
    * **<color #00a2e8>Dose</color>**: 10nM
    * **<color #00a2e8>Days of incubation</color>**: 8
    * **<color #00a2e8>Doublings</color>**: 7.3
    * **<color #00a2e8>Numbers of reads</color>**: 18854286


==== Screen Results ====


^Sensitive/Resistant hits (FDR<0.05)^CRANKS^Score Plot^Top 30 Genes^Screen Similarity^Top 30 Sensitive GO terms^Top 30 Resistant GO terms^
|0/0|<color #e85500>[[https://files.tyerslab.com/files/public/chemogenomics/cranks/Geldanamycin_0.01uM_Round-5_exp235.txt|Scores]]</color>|<button type="primary" size="sm" modal="ScorePlot">View</button>|<button type="primary" size="sm" modal="modal_hits">View</button>|<button type="primary" size="sm" modal="modal_screen_similarity">View</button>|<button type="default" size="sm" modal="modal_go_sensitive">View</button>|<button type="primary" size="sm" modal="modal_go_resistant">View</button>|
<modal id='ScorePlot' size='lg' title='Geldanamycin 0.01μM R05 exp235'>
{{:chemogenomics:cranks_plots:exp235.png?nolink}}
</modal>


<modal id="modal_hits" size="sm" title="Top 30 S/R Genes">
^Gene^CRANKS Score^FDR^
|[[:human_genes:P:PDSS1|PDSS1]]|-2.63|0.21|
|[[:human_genes:O:ODC1|ODC1]]|-2.51|0.21|
|[[:human_genes:A:ATP2B1|ATP2B1]]|-2.49|0.21|
|[[:human_genes:R:RHOA|RHOA]]|-2.37|0.21|
|[[:human_genes:G:GCLC|GCLC]]|-2.35|0.27|
|[[:human_genes:A:ADSL|ADSL]]|-2.34|0.56|
|[[:human_genes:D:DDX39A|DDX39A]]|-2.34|0.21|
|[[:human_genes:R:RBMX2|RBMX2]]|-2.30|0.29|
|[[:human_genes:M:MARS|MARS]]|-2.23|0.74|
|[[:human_genes:M:MT3|MT3]]|-2.20|0.27|
|[[:human_genes:P:PAXIP1|PAXIP1]]|-2.20|0.27|
|[[:human_genes:P:PRAMEF25|PRAMEF25]]|-2.19|0.77|
|[[:human_genes:C:C6orf1|C6orf1]]|-2.18|0.41|
|[[:human_genes:P:PRAMEF26|PRAMEF26]]|-2.16|0.79|
|[[:human_genes:A:AGO2|AGO2]]|-2.14|0.44|
|[[:human_genes:V:VPS26A|VPS26A]]|-2.13|0.32|
|[[:human_genes:D:DNAJB11|DNAJB11]]|-2.08|0.39|
|[[:human_genes:Z:ZP3|ZP3]]|-2.07|0.54|
|[[:human_genes:Q:QARS|QARS]]|-2.05|0.71|
|[[:human_genes:G:GDAP1|GDAP1]]|-2.02|0.44|
|[[:human_genes:F:FAM186A|FAM186A]]|-2.02|0.59|
|[[:human_genes:S:SGIP1|SGIP1]]|-1.99|0.65|
|[[:human_genes:G:GLI4|GLI4]]|-1.98|0.51|
|[[:human_genes:T:TAZ|TAZ]]|-1.95|0.73|
|[[:human_genes:P:PGGT1B|PGGT1B]]|-1.94|0.56|
|[[:human_genes:G:GMPPB|GMPPB]]|-1.94|0.70|
|[[:human_genes:C:C21orf59|C21orf59]]|-1.91|0.62|
|[[:human_genes:N:NEUROD1|NEUROD1]]|-1.90|0.62|
|[[:human_genes:M:MPHOSPH9|MPHOSPH9]]|-1.89|0.61|
|[[:human_genes:T:TRPC1|TRPC1]]|-1.88|0.67|
|[[:human_genes:A:AS3MT|AS3MT]]|1.95|0.54|
|[[:human_genes:G:GAGE2A|GAGE2A]]|1.96|0.79|
|[[:human_genes:T:TP53I11|TP53I11]]|1.97|0.48|
|[[:human_genes:N:NOL8|NOL8]]|1.97|0.48|
|[[:human_genes:M:MOS|MOS]]|1.97|0.48|
|[[:human_genes:T:TAF6|TAF6]]|1.97|0.57|
|[[:human_genes:N:NFIL3|NFIL3]]|1.98|0.48|
|[[:human_genes:P:PSMB4|PSMB4]]|2.00|0.54|
|[[:human_genes:T:TTC9|TTC9]]|2.00|0.48|
|[[:human_genes:M:MKNK1|MKNK1]]|2.01|0.55|
|[[:human_genes:T:TEX12|TEX12]]|2.03|0.55|
|[[:human_genes:W:WNK1|WNK1]]|2.03|0.55|
|[[:human_genes:P:PSMC3|PSMC3]]|2.04|0.65|
|[[:human_genes:C:CDC20|CDC20]]|2.10|0.54|
|[[:human_genes:I:ITGB1BP2|ITGB1BP2]]|2.20|0.29|
|[[:human_genes:P:PSMD11|PSMD11]]|2.26|0.35|
|[[:human_genes:B:BYSL|BYSL]]|2.29|0.48|
|[[:human_genes:S:SIPA1L3|SIPA1L3]]|2.32|0.16|
|[[:human_genes:C:CKAP5|CKAP5]]|2.34|0.55|
|[[:human_genes:E:EDA2R|EDA2R]]|2.34|0.16|
|[[:human_genes:I:INCENP|INCENP]]|2.37|0.39|
|[[:human_genes:S:SCAF1|SCAF1]]|2.40|0.63|
|[[:human_genes:T:TLL1|TLL1]]|2.40|0.16|
|[[:human_genes:L:LIG1|LIG1]]|2.41|0.63|
|[[:human_genes:U:UBAP2|UBAP2]]|2.45|0.16|
|[[:human_genes:P:PSMD1|PSMD1]]|2.45|0.33|
|[[:human_genes:S:SMC2|SMC2]]|2.46|0.48|
|[[:human_genes:C:CCDC34|CCDC34]]|2.52|0.16|
|[[:human_genes:P:PIK3CG|PIK3CG]]|2.52|0.16|
|[[:human_genes:Z:ZIC1|ZIC1]]|2.83|0.16|
</modal>


<modal id="modal_screen_similarity" size="lg" title="Correlated Screens">
^Screen^Correlation^Plot^
|[[:results:exp249|Vinorelbine 0.001μM R05 exp249]]|0.062|<button type="primary" size="small" modal="1">View</button>|
|[[:results:exp286|HMS-I2 1μM R06 exp286]]|0.053|<button type="primary" size="small" modal="2">View</button>|
|[[:results:exp15|Cycloheximide 0.2μM R00 exp15]]|0.065|<button type="primary" size="small" modal="3">View</button>|
</modal>


<modal id="1" size="lg" title="Geldanamycin 0.01μM R05 exp235  vs  Vinorelbine 0.001μM R05 exp249">
{{:chemogenomics:comparison_plots:exp235_vs_exp249.png?nolink |}}
</modal>

<modal id="2" size="lg" title="Geldanamycin 0.01μM R05 exp235  vs  HMS-I2 1μM R06 exp286">
{{:chemogenomics:comparison_plots:exp235_vs_exp286.png?nolink |}}
</modal>

<modal id="3" size="lg" title="Cycloheximide 0.2μM R00 exp15  vs  Geldanamycin 0.01μM R05 exp235">
{{:chemogenomics:comparison_plots:exp15_vs_exp235.png?nolink |}}
</modal>



<modal id="modal_go_sensitive" size="lg" title="Top 30 sensitive GO terms">No GO term hits below threshold. (FDR <0.05)</modal>
<modal id="modal_go_resistant" size="lg" title="Top 30 resistant GO terms">
^GO Term^Fold Change^Genes^
|proteasome regulatory particle|97.47|PSMD1,PSMD11,PSMC3|
|positive regulation of chromosome separation|61.37|SMC2,INCENP,CDC20|
|proteasome complex|37.45|PSMD1,PSMD11,PSMC3,PSMB4|
|regulation of chromosome separation|30.69|SMC2,INCENP,CDC20,MOS|
|endopeptidase complex|26.62|PSMD1,PSMD11,PSMC3,PSMB4|
|peptidase complex|23.40|PSMD1,PSMD11,PSMC3,PSMB4,TAF6|
|meiotic cell cycle process|15.87|SMC2,INCENP,CDC20,TEX12,MOS|
</modal>