Oligomycin-A 2μM R00 exp25 [The Human Chemical-Genetic Interaction Map Project]

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====== Oligomycin-A 2μM R00 exp25 ======
==== Mechanism of Action ====
Inhibits F0 proton channel of mitochondrial ATP synthase, blocks electron flow through ETC
  * **<color #00a2e8>Class / Subclass 1:</color>** Organelle Function / Mitochondrial Inhibitor




==== Technical Notes ====
<accordion collapsed='true'>
<panel title='Compound References' collapsed='true'>
    * **<color #00a2e8>PubChem Name:</color>** %%Oligomycin A%%
    * **<color #00a2e8>Synonyms:</color>** MCH 32
    * **<color #00a2e8>CAS #:</color>** 579-13-5
    * **<color #00a2e8>PubChem CID:</color>** [[https://pubchem.ncbi.nlm.nih.gov/compound/5281899|5281899]]
    * **<color #00a2e8>IUPAC:</color>** %%(1S,4E,5'R,6R,6'R,7S,8R,10S,11S,12R,14S,15R,16S,18E,20E,22S,25R,27S,28R,29S)-22-ethyl-7,11,14,15-tetrahydroxy-6'-[(2S)-2-hydroxypropyl]-5',6,8,10,12,14,16,28,29-nonamethylspiro[2,26-dioxabicyclo[23.3.1]nonacosa-4,18,20-triene-27,2'-oxane]-3,9,13-trione%%
    * **<color #00a2e8>INCHI Name:</color>** InChI=1S/C45H74O11/c1-12-34-17-15-13-14-16-27(4)42(51)44(11,53)43(52)32(9)40(50)31(8)39(49)30(7)38(48)26(3)18-21-37(47)54-41-29(6)35(20-19-34)55-45(33(41)10)23-22-25(2)36(56-45)24-28(5)46/h13-15,17-18,21,25-36,38,40-42,46,48,50-51,53H,12,16,19-20,22-24H2,1-11H3/b14-13+,17-15+,21-18+/t25-,26-,27+,28+,29+,30-,31-,32-,33-,34-,35-,36-,38+,40+,41+,42-,44+,45-/m1/s1
    * **<color #00a2e8>INCHI Key:</color>** MNULEGDCPYONBU-WMBHJXFZSA-N
    * **<color #00a2e8>Molecular Weight:</color>** 791.1
    * **<color #00a2e8>Canonical SMILES:</color>** CCC1CCC2C(C(C(C3(O2)CCC(C(O3)CC(C)O)C)C)OC(=O)C=CC(C(C(C(=O)C(C(C(C(=O)C(C(C(CC=CC=C1)C)O)(C)O)C)O)C)C)O)C)C
    * **<color #00a2e8>Isomeric SMILES:</color>** CC[C@H]\\1CC[C@@H]2[C@@H]([C@@H]([C@H]([C@@]3(O2)CC[C@H]([C@H](O3)C[C@H](C)O)C)C)OC(=O)/C=C/[C@H]([C@@H]([C@H](C(=O)[C@H]([C@@H]([C@H](C(=O)[C@@]([C@@H]([C@H](C/C=C/C=C1)C)O)(C)O)C)O)C)C)O)C)C
    * **<color #00a2e8>Molecular Formula:</color>** C45H74O11
{{:chemogenomics:structures:chem-0014.svg?nolink}}
</panel>


<panel title='Compound Supplier' collapsed='true'>
    * **<color #00a2e8>Supplier Name:</color>** Tocris Bioscience
    * **<color #00a2e8>Catalog #:</color>** 4110
    * **<color #00a2e8>Lot #:</color>** 6A/200926
</panel>


<panel title='Compound Characterization' collapsed='true'>
    * **<color #00a2e8>HRMS (ESI-TOF) m/z:</color>** (M+H)+ Calcd for C45H74O11 791.53039; found 791.53025
</panel>


<panel title='Dose Response Curve'>
Dose response curve not available.
</panel>


</accordion>
==== Screen Summary ====
    * **<color #00a2e8>Round</color>**: 00
    * **<color #00a2e8>Dose</color>**: 2µM
    * **<color #00a2e8>Days of incubation</color>**: 8
    * **<color #00a2e8>Doublings</color>**: 4.1
    * **<color #00a2e8>Numbers of reads</color>**: 12775855


==== Screen Results ====


^Sensitive/Resistant hits (FDR<0.05)^CRANKS^Score Plot^Top 30 Genes^Screen Similarity^Top 30 Sensitive GO terms^Top 30 Resistant GO terms^
|19/188|<color #e85500>[[https://files.tyerslab.com/files/public/chemogenomics/cranks/Oligomycin_A_2uM_Round-0_exp25.txt|Scores]]</color>|<button type="primary" size="sm" modal="ScorePlot">View</button>|<button type="primary" size="sm" modal="modal_hits">View</button>|<button type="primary" size="sm" modal="modal_screen_similarity">View</button>|<button type="default" size="sm" modal="modal_go_sensitive">View</button>|<button type="primary" size="sm" modal="modal_go_resistant">View</button>|
<modal id='ScorePlot' size='lg' title='Oligomycin-A 2μM R00 exp25'>
{{:chemogenomics:cranks_plots:exp25.png?nolink}}
</modal>


<modal id="modal_hits" size="sm" title="Top 30 S/R Genes">
^Gene^CRANKS Score^FDR^
|[[:human_genes:P:PARL|PARL]]|-4.71|<0.01|
|[[:human_genes:T:TCF4|TCF4]]|-4.49|<0.01|
|[[:human_genes:P:PRKAG1|PRKAG1]]|-4.12|<0.01|
|[[:human_genes:S:SOX4|SOX4]]|-3.55|<0.01|
|[[:human_genes:S:SPG7|SPG7]]|-3.34|<0.01|
|[[:human_genes:C:COX7A2L|COX7A2L]]|-3.09|<0.01|
|[[:human_genes:U:UXS1|UXS1]]|-3.04|<0.01|
|[[:human_genes:P:PSMA5|PSMA5]]|-2.97|0.04|
|[[:human_genes:P:PRKAA1|PRKAA1]]|-2.81|<0.01|
|[[:human_genes:K:KCMF1|KCMF1]]|-2.77|<0.01|
|[[:human_genes:P:PSPC1|PSPC1]]|-2.75|<0.01|
|[[:human_genes:L:LRRC16A|LRRC16A]]|-2.74|<0.01|
|[[:human_genes:P:PGP|PGP]]|-2.66|0.01|
|[[:human_genes:C:CDCA8|CDCA8]]|-2.59|0.04|
|[[:human_genes:D:DNAJC7|DNAJC7]]|-2.59|0.04|
|[[:human_genes:S:SLC25A5|SLC25A5]]|-2.55|0.02|
|[[:human_genes:P:PCBP2|PCBP2]]|-2.44|0.04|
|[[:human_genes:N:NME6|NME6]]|-2.36|0.05|
|[[:human_genes:M:MRS2|MRS2]]|-2.36|0.05|
|[[:human_genes:S:SRF|SRF]]|-2.35|0.08|
|[[:human_genes:S:SOX7|SOX7]]|-2.35|0.08|
|[[:human_genes:E:ELF2|ELF2]]|-2.33|0.05|
|[[:human_genes:Z:ZNF319|ZNF319]]|-2.32|0.05|
|[[:human_genes:A:ACACA|ACACA]]|-2.32|0.09|
|[[:human_genes:D:DNAH17|DNAH17]]|-2.29|0.14|
|[[:human_genes:L:LDHB|LDHB]]|-2.29|0.09|
|[[:human_genes:A:ARHGEF10|ARHGEF10]]|-2.29|0.06|
|[[:human_genes:W:WT1|WT1]]|-2.27|0.07|
|[[:human_genes:V:VPS53|VPS53]]|-2.26|0.21|
|[[:human_genes:P:PFKP|PFKP]]|-2.25|0.07|
|[[:human_genes:D:DDX3X|DDX3X]]|3.52|<0.01|
|[[:human_genes:N:NDUFAF6|NDUFAF6]]|3.53|<0.01|
|[[:human_genes:N:NDUFAF1|NDUFAF1]]|3.54|<0.01|
|[[:human_genes:P:PPP3R1|PPP3R1]]|3.58|<0.01|
|[[:human_genes:T:TFB1M|TFB1M]]|3.58|<0.01|
|[[:human_genes:N:NDUFC2|NDUFC2]]|3.60|<0.01|
|[[:human_genes:N:NDUFB10|NDUFB10]]|3.62|<0.01|
|[[:human_genes:N:NDUFS2|NDUFS2]]|3.63|<0.01|
|[[:human_genes:Y:YLPM1|YLPM1]]|3.68|<0.01|
|[[:human_genes:T:TMEM261|TMEM261]]|3.78|<0.01|
|[[:human_genes:N:NDUFS5|NDUFS5]]|3.80|<0.01|
|[[:human_genes:B:BRD1|BRD1]]|3.80|<0.01|
|[[:human_genes:N:NDUFB8|NDUFB8]]|3.80|<0.01|
|[[:human_genes:N:NDUFA3|NDUFA3]]|3.82|<0.01|
|[[:human_genes:R:RTN4IP1|RTN4IP1]]|3.86|<0.01|
|[[:human_genes:C:C10orf137|C10orf137]]|3.87|<0.01|
|[[:human_genes:P:PET117|PET117]]|3.91|<0.01|
|[[:human_genes:G:GRSF1|GRSF1]]|3.98|<0.01|
|[[:human_genes:N:NDUFB11|NDUFB11]]|3.99|<0.01|
|[[:human_genes:S:SLC25A51|SLC25A51]]|4.24|<0.01|
|[[:human_genes:A:ACAD9|ACAD9]]|4.31|<0.01|
|[[:human_genes:T:TXNIP|TXNIP]]|4.37|<0.01|
|[[:human_genes:N:NDUFA11|NDUFA11]]|4.42|<0.01|
|[[:human_genes:N:NDUFA2|NDUFA2]]|4.50|<0.01|
|[[:human_genes:N:NDUFA1|NDUFA1]]|5.25|<0.01|
|[[:human_genes:R:RBM26|RBM26]]|5.30|<0.01|
|[[:human_genes:T:TIMM21|TIMM21]]|5.62|<0.01|
|[[:human_genes:C:CLUH|CLUH]]|5.98|<0.01|
|[[:human_genes:S:STARD7|STARD7]]|8.30|<0.01|
|[[:human_genes:O:OMA1|OMA1]]|8.37|<0.01|
</modal>


<modal id="modal_screen_similarity" size="lg" title="Correlated Screens">
^Screen^Correlation^Plot^
|[[:results:exp84|UM0125461 0.74μM R02 exp84]]|0.118|<button type="primary" size="small" modal="1">View</button>|
|[[:results:exp529|Thimerosal 0.85μM R08 exp529]]|0.1|<button type="primary" size="small" modal="2">View</button>|
|[[:results:exp492|iCRT14 30μM R08 exp492]]|0.095|<button type="primary" size="small" modal="3">View</button>|
|[[:results:exp30|Rapamycin 10μM R00 exp30]]|0.062|<button type="primary" size="small" modal="4">View</button>|
|[[:results:exp160|Ribavirin 10 to 15μM on day4 R04 exp160]]|0.055|<button type="primary" size="small" modal="5">View</button>|
|[[:results:exp416|Tubacin 1.6μM R07 exp416]]|0.054|<button type="primary" size="small" modal="6">View</button>|
|[[:results:exp29|Rapamycin 1μM R00 exp29]]|0.054|<button type="primary" size="small" modal="7">View</button>|
|[[:results:exp434|Vemurafenib 6.6μM R08 exp434]]|0.054|<button type="primary" size="small" modal="8">View</button>|
|[[:results:exp288|HMS-I2 10μM R06 exp288]]|0.124|<button type="primary" size="small" modal="9">View</button>|
|[[:results:exp329|Hydroxyurea 100μM R07 exp329]]|0.109|<button type="primary" size="small" modal="10">View</button>|
|[[:results:exp450|Artemisinin 50μM R08 exp450]]|0.097|<button type="primary" size="small" modal="11">View</button>|
|[[:results:exp342|Calcium Ionophore 0.4μM R07 exp342]]|0.095|<button type="primary" size="small" modal="12">View</button>|
|[[:results:exp451|Atovaquone 15μM R08 exp451]]|0.09|<button type="primary" size="small" modal="13">View</button>|
|[[:results:exp360|Genistein 15μM R07 exp360]]|0.088|<button type="primary" size="small" modal="14">View</button>|
|[[:results:exp65|Mubritinib 0.2μM R02 exp65]]|0.068|<button type="primary" size="small" modal="15">View</button>|
|[[:results:exp13|Chloramphenicol 20μM R00 exp13]]|0.065|<button type="primary" size="small" modal="16">View</button>|
|[[:results:exp290|LLY-283 2.6μM R06 exp290]]|0.06|<button type="primary" size="small" modal="17">View</button>|
|[[:results:exp48|Mubritinib 0.2μM R01 exp48]]|0.055|<button type="primary" size="small" modal="18">View</button>|
|[[:results:exp488|Hippuristanol 0.12μM R08 exp488]]|0.052|<button type="primary" size="small" modal="19">View</button>|
</modal>


<modal id="1" size="lg" title="Oligomycin-A 2μM R00 exp25  vs  UM0125461 0.74μM R02 exp84">
{{:chemogenomics:comparison_plots:exp25_vs_exp84.png?nolink |}}
</modal>

<modal id="2" size="lg" title="Oligomycin-A 2μM R00 exp25  vs  Thimerosal 0.85μM R08 exp529">
{{:chemogenomics:comparison_plots:exp25_vs_exp529.png?nolink |}}
</modal>

<modal id="3" size="lg" title="Oligomycin-A 2μM R00 exp25  vs  iCRT14 30μM R08 exp492">
{{:chemogenomics:comparison_plots:exp25_vs_exp492.png?nolink |}}
</modal>

<modal id="4" size="lg" title="Oligomycin-A 2μM R00 exp25  vs  Rapamycin 10μM R00 exp30">
{{:chemogenomics:comparison_plots:exp25_vs_exp30.png?nolink |}}
</modal>

<modal id="5" size="lg" title="Oligomycin-A 2μM R00 exp25  vs  Ribavirin 10 to 15μM on day4 R04 exp160">
{{:chemogenomics:comparison_plots:exp160_vs_exp25.png?nolink |}}
</modal>

<modal id="6" size="lg" title="Oligomycin-A 2μM R00 exp25  vs  Tubacin 1.6μM R07 exp416">
{{:chemogenomics:comparison_plots:exp25_vs_exp416.png?nolink |}}
</modal>

<modal id="7" size="lg" title="Oligomycin-A 2μM R00 exp25  vs  Rapamycin 1μM R00 exp29">
{{:chemogenomics:comparison_plots:exp25_vs_exp29.png?nolink |}}
</modal>

<modal id="8" size="lg" title="Oligomycin-A 2μM R00 exp25  vs  Vemurafenib 6.6μM R08 exp434">
{{:chemogenomics:comparison_plots:exp25_vs_exp434.png?nolink |}}
</modal>

<modal id="9" size="lg" title="HMS-I2 10μM R06 exp288  vs  Oligomycin-A 2μM R00 exp25">
{{:chemogenomics:comparison_plots:exp25_vs_exp288.png?nolink |}}
</modal>

<modal id="10" size="lg" title="Hydroxyurea 100μM R07 exp329  vs  Oligomycin-A 2μM R00 exp25">
{{:chemogenomics:comparison_plots:exp25_vs_exp329.png?nolink |}}
</modal>

<modal id="11" size="lg" title="Artemisinin 50μM R08 exp450  vs  Oligomycin-A 2μM R00 exp25">
{{:chemogenomics:comparison_plots:exp25_vs_exp450.png?nolink |}}
</modal>

<modal id="12" size="lg" title="Calcium Ionophore 0.4μM R07 exp342  vs  Oligomycin-A 2μM R00 exp25">
{{:chemogenomics:comparison_plots:exp25_vs_exp342.png?nolink |}}
</modal>

<modal id="13" size="lg" title="Atovaquone 15μM R08 exp451  vs  Oligomycin-A 2μM R00 exp25">
{{:chemogenomics:comparison_plots:exp25_vs_exp451.png?nolink |}}
</modal>

<modal id="14" size="lg" title="Genistein 15μM R07 exp360  vs  Oligomycin-A 2μM R00 exp25">
{{:chemogenomics:comparison_plots:exp25_vs_exp360.png?nolink |}}
</modal>

<modal id="15" size="lg" title="Mubritinib 0.2μM R02 exp65  vs  Oligomycin-A 2μM R00 exp25">
{{:chemogenomics:comparison_plots:exp25_vs_exp65.png?nolink |}}
</modal>

<modal id="16" size="lg" title="Chloramphenicol 20μM R00 exp13  vs  Oligomycin-A 2μM R00 exp25">
{{:chemogenomics:comparison_plots:exp13_vs_exp25.png?nolink |}}
</modal>

<modal id="17" size="lg" title="LLY-283 2.6μM R06 exp290  vs  Oligomycin-A 2μM R00 exp25">
{{:chemogenomics:comparison_plots:exp25_vs_exp290.png?nolink |}}
</modal>

<modal id="18" size="lg" title="Mubritinib 0.2μM R01 exp48  vs  Oligomycin-A 2μM R00 exp25">
{{:chemogenomics:comparison_plots:exp25_vs_exp48.png?nolink |}}
</modal>

<modal id="19" size="lg" title="Hippuristanol 0.12μM R08 exp488  vs  Oligomycin-A 2μM R00 exp25">
{{:chemogenomics:comparison_plots:exp25_vs_exp488.png?nolink |}}
</modal>



<modal id="modal_go_sensitive" size="lg" title="Top 30 sensitive GO terms">No GO term hits below threshold. (FDR <0.05)</modal>
<modal id="modal_go_resistant" size="lg" title="Top 30 resistant GO terms">
^GO Term^Fold Change^Genes^
|mitochondrial respiratory chain complex I assembly|159.44|TIMM21,NDUFA1,NDUFA2,NDUFA11,ACAD9,NDUFB11,NDUFA3,NDUFB8,NDUFS5,DMAC1,NDUFS2,NDUFB10,NDUFC2,NDUFAF1,NDUFAF6|
|NADH dehydrogenase complex assembly|159.44|TIMM21,NDUFA1,NDUFA2,NDUFA11,ACAD9,NDUFB11,NDUFA3,NDUFB8,NDUFS5,DMAC1,NDUFS2,NDUFB10,NDUFC2,NDUFAF1,NDUFAF6|
|mitochondrial respiratory chain complex I|144.76|NDUFA1,NDUFA2,NDUFA11,NDUFB11,NDUFA3,NDUFB8,NDUFS5,NDUFS2,NDUFB10,NDUFC2,NDUFAF1|
|respiratory chain complex I|134.81|NDUFA1,NDUFA2,NDUFA11,NDUFB11,NDUFA3,NDUFB8,NDUFS5,NDUFS2,NDUFC2,NDUFAF1|
|NADH dehydrogenase complex|134.81|NDUFA1,NDUFA2,NDUFA11,NDUFB11,NDUFA3,NDUFB8,NDUFS5,NDUFS2,NDUFB10,NDUFC2|
|mitochondrial electron transport, NADH to ubiquinone|130.91|NDUFA1,NDUFA2,NDUFA3,NDUFB8,NDUFS5,NDUFS2,NDUFB10,NDUFC2,NDUFAF1|
|mitochondrial respiratory chain complex assembly|108.01|OMA1,TIMM21,NDUFA1,NDUFA2,NDUFA11,ACAD9,NDUFB11,PET117,NDUFA3,NDUFB8,NDUFS5,DMAC1,NDUFS2,NDUFB10,NDUFC2,NDUFAF1,NDUFAF6|
|proton motive force-driven ATP synthesis|92.12|NDUFA1,NDUFA2,NDUFA11,NDUFB11,NDUFA3,NDUFB8,NDUFS5,NDUFS2,NDUFB10,NDUFC2|
|respiratory chain complex|82.16|NDUFA1,NDUFA2,NDUFA11,NDUFB11,NDUFA3,NDUFB8,NDUFS5,NDUFS2,NDUFB10,NDUFC2,NDUFAF1|
|mitochondrial respirasome|81.07|NDUFA1,NDUFA2,NDUFA11,NDUFB11,NDUFA3,NDUFB8,NDUFS5,NDUFS2,NDUFB10,NDUFC2,NDUFAF1|
|respirasome|74.15|NDUFA1,NDUFA2,NDUFA11,NDUFB11,NDUFA3,NDUFB8,NDUFS5,NDUFS2,NDUFB10,NDUFC2,NDUFAF1|
|oxidative phosphorylation|67.00|NDUFA1,NDUFA2,NDUFA11,SLC25A51,NDUFB11,NDUFA3,NDUFB8,NDUFS5,NDUFS2,NDUFB10,NDUFC2,NDUFAF1|
|inner mitochondrial membrane protein complex|49.87|TIMM21,NDUFA1,NDUFA2,NDUFA11,NDUFB11,NDUFA3,NDUFB8,NDUFS5,NDUFS2,NDUFB10,NDUFC2,NDUFAF1|
|aerobic respiration|47.04|NDUFA1,NDUFA2,NDUFA11,SLC25A51,NDUFB11,NDUFA3,NDUFB8,NDUFS5,NDUFS2,NDUFB10,NDUFC2,NDUFAF1|
|mitochondrion organization|23.63|OMA1,CLUH,TIMM21,NDUFA1,NDUFA2,NDUFA11,ACAD9,NDUFB11,PET117,NDUFA3,NDUFB8,NDUFS5,DMAC1,NDUFS2,NDUFB10,NDUFC2,NDUFAF1,NDUFAF6|
|mitochondrial inner membrane|21.68|OMA1,TIMM21,NDUFA1,NDUFA2,NDUFA11,ACAD9,SLC25A51,NDUFB11,NDUFA3,NDUFB8,NDUFS5,DMAC1,NDUFS2,NDUFB10,NDUFC2,NDUFAF1,NDUFAF6,TIMMDC1|
|organelle inner membrane|19.47|OMA1,TIMM21,NDUFA1,NDUFA2,NDUFA11,ACAD9,SLC25A51,NDUFB11,NDUFA3,NDUFB8,NDUFS5,DMAC1,NDUFS2,NDUFB10,NDUFC2,NDUFAF1,NDUFAF6,TIMMDC1|
|mitochondrial membrane|15.66|OMA1,STARD7,TIMM21,NDUFA1,NDUFA2,NDUFA11,ACAD9,SLC25A51,NDUFB11,RTN4IP1,NDUFA3,NDUFB8,NDUFS5,DMAC1,NDUFS2,NDUFB10,NDUFC2,NDUFAF1,NDUFAF6,TIMMDC1|
|mitochondrial envelope|14.70|OMA1,STARD7,TIMM21,NDUFA1,NDUFA2,NDUFA11,ACAD9,SLC25A51,NDUFB11,RTN4IP1,NDUFA3,NDUFB8,NDUFS5,DMAC1,NDUFS2,NDUFB10,NDUFC2,NDUFAF1,NDUFAF6,TIMMDC1|
</modal>