Show pageOld revisionsBacklinksFold/unfold allBack to top This page is read only. You can view the source, but not change it. Ask your administrator if you think this is wrong. ====== Rapamycin 10μM R00 exp30 ====== ==== Mechanism of Action ==== Allosteric mTORC1 inhibitor, forms ternary complex with FKBP12 and mTOR, attenuates protein synthesis and metabolism * **<color #00a2e8>Class / Subclass 1:</color>** Signal Transduction / Kinase Inhibitor * **<color #00a2e8>Class / Subclass 2:</color>** Proteostasis / mTOR inhibitor ==== Technical Notes ==== <accordion collapsed='true'> <panel title='Compound References' collapsed='true'> * **<color #00a2e8>PubChem Name:</color>** %%Sirolimus%% * **<color #00a2e8>Synonyms:</color>** Sirolimus; AY 22989 * **<color #00a2e8>CAS #:</color>** 53123-88-9 * **<color #00a2e8>PubChem CID:</color>** [[https://pubchem.ncbi.nlm.nih.gov/compound/5284616|5284616]] * **<color #00a2e8>IUPAC:</color>** %%(1R,9S,12S,15R,16E,18R,19R,21R,23S,24E,26E,28E,30S,32S,35R)-1,18-dihydroxy-12-[(2R)-1-[(1S,3R,4R)-4-hydroxy-3-methoxycyclohexyl]propan-2-yl]-19,30-dimethoxy-15,17,21,23,29,35-hexamethyl-11,36-dioxa-4-azatricyclo[30.3.1.04,9]hexatriaconta-16,24,26,28-tetraene-2,3,10,14,20-pentone%% * **<color #00a2e8>INCHI Name:</color>** InChI=1S/C51H79NO13/c1-30-16-12-11-13-17-31(2)42(61-8)28-38-21-19-36(7)51(60,65-38)48(57)49(58)52-23-15-14-18-39(52)50(59)64-43(33(4)26-37-20-22-40(53)44(27-37)62-9)29-41(54)32(3)25-35(6)46(56)47(63-10)45(55)34(5)24-30/h11-13,16-17,25,30,32-34,36-40,42-44,46-47,53,56,60H,14-15,18-24,26-29H2,1-10H3/b13-11+,16-12+,31-17+,35-25+/t30-,32-,33-,34-,36-,37+,38+,39+,40-,42+,43+,44-,46-,47+,51-/m1/s1 * **<color #00a2e8>INCHI Key:</color>** QFJCIRLUMZQUOT-HPLJOQBZSA-N * **<color #00a2e8>Molecular Weight:</color>** 914.2 * **<color #00a2e8>Canonical SMILES:</color>** CC1CCC2CC(C(=CC=CC=CC(CC(C(=O)C(C(C(=CC(C(=O)CC(OC(=O)C3CCCCN3C(=O)C(=O)C1(O2)O)C(C)CC4CCC(C(C4)OC)O)C)C)O)OC)C)C)C)OC * **<color #00a2e8>Isomeric SMILES:</color>** C[C@@H]1CC[C@H]2C[C@@H](/C(=C/C=C/C=C/[C@H](C[C@H](C(=O)[C@@H]([C@@H](/C(=C/[C@H](C(=O)C[C@H](OC(=O)[C@@H]3CCCCN3C(=O)C(=O)[C@@]1(O2)O)[C@H](C)C[C@@H]4CC[C@H]([C@@H](C4)OC)O)C)/C)O)OC)C)C)/C)OC * **<color #00a2e8>Molecular Formula:</color>** C51H79NO13 {{:chemogenomics:structures:chem-0016.svg?nolink}} </panel> <panel title='Compound Supplier' collapsed='true'> * **<color #00a2e8>Supplier Name:</color>** LC Laboratories * **<color #00a2e8>Catalog #:</color>** R-5000 * **<color #00a2e8>Lot #:</color>** ASW-130 </panel> <panel title='Compound Characterization' collapsed='true'> * **<color #00a2e8>HRMS (ESI-TOF) m/z:</color>** (M+Na)+ Calcd for C51H79NO13 936.54436; found 936.54366 </panel> <panel title='Dose Response Curve' collapsed='true'> * **<color #00a2e8>Platform ID:</color>** Rapamycin * **<color #00a2e8>Min:</color>** 29.4254; **<color #00a2e8>Max:</color>** 49.7567 {{:chemogenomics:dose_response:dr_273.png?nolink&500 |}} \\ \\ \\ \\ |< 300px 100px 200px >| ^ IC ^ Concentration (µM) ^ | IC10 |N/A | | IC20 |N/A | | IC30 |N/A | | IC40 |N/A | | IC50 |N/A | | IC60 |N/A | | IC70 |N/A | | IC80 |N/A | | IC90 |N/A | \\ </panel> </accordion> ==== Screen Summary ==== * **<color #00a2e8>Round</color>**: 00 * **<color #00a2e8>Dose</color>**: 10µM * **<color #00a2e8>Days of incubation</color>**: 8 * **<color #00a2e8>Doublings</color>**: 3.5 * **<color #00a2e8>Numbers of reads</color>**: 10555392 ==== Screen Results ==== ^Sensitive/Resistant hits (FDR<0.05)^CRANKS^Score Plot^Top 30 Genes^Screen Similarity^Top 30 Sensitive GO terms^Top 30 Resistant GO terms^ |20/68|<color #e85500>[[https://files.tyerslab.com/files/public/chemogenomics/cranks/Rapamycin_10uM_Round-0_exp30.txt|Scores]]</color>|<button type="primary" size="sm" modal="ScorePlot">View</button>|<button type="primary" size="sm" modal="modal_hits">View</button>|<button type="primary" size="sm" modal="modal_screen_similarity">View</button>|<button type="default" size="sm" modal="modal_go_sensitive">View</button>|<button type="primary" size="sm" modal="modal_go_resistant">View</button>| <modal id='ScorePlot' size='lg' title='Rapamycin 10μM R00 exp30'> {{:chemogenomics:cranks_plots:exp30.png?nolink}} </modal> <modal id="modal_hits" size="sm" title="Top 30 S/R Genes"> ^Gene^CRANKS Score^FDR^ |[[:human_genes:A:ABCC1|ABCC1]]|-7.22|<0.01| |[[:human_genes:T:TMEM30A|TMEM30A]]|-4.05|<0.01| |[[:human_genes:U:UXS1|UXS1]]|-3.76|<0.01| |[[:human_genes:S:SENP1|SENP1]]|-3.49|<0.01| |[[:human_genes:G:GPR107|GPR107]]|-2.97|<0.01| |[[:human_genes:Z:ZBTB10|ZBTB10]]|-2.82|0.02| |[[:human_genes:M:MEF2A|MEF2A]]|-2.74|0.02| |[[:human_genes:D:DSCAM|DSCAM]]|-2.74|0.01| |[[:human_genes:T:TAF5L|TAF5L]]|-2.70|0.02| |[[:human_genes:R:RHOA|RHOA]]|-2.67|0.02| |[[:human_genes:S:SEC31A|SEC31A]]|-2.64|0.02| |[[:human_genes:M:MEF2C|MEF2C]]|-2.63|0.02| |[[:human_genes:D:DERL2|DERL2]]|-2.58|0.03| |[[:human_genes:S:SPAG7|SPAG7]]|-2.57|0.02| |[[:human_genes:T:TRIP12|TRIP12]]|-2.56|0.02| |[[:human_genes:T:TCF4|TCF4]]|-2.56|0.02| |[[:human_genes:L:LAMTOR4|LAMTOR4]]|-2.53|0.04| |[[:human_genes:X:XPO5|XPO5]]|-2.46|0.13| |[[:human_genes:I:ID3|ID3]]|-2.44|0.03| |[[:human_genes:E:EIF5B|EIF5B]]|-2.44|0.13| |[[:human_genes:C:CAMKV|CAMKV]]|-2.41|0.07| |[[:human_genes:A:ATXN2L|ATXN2L]]|-2.39|0.07| |[[:human_genes:A:ASB3|ASB3]]|-2.39|0.04| |[[:human_genes:A:ATP8B2|ATP8B2]]|-2.37|0.11| |[[:human_genes:S:SCYL1|SCYL1]]|-2.36|0.08| |[[:human_genes:T:TIPRL|TIPRL]]|-2.34|0.05| |[[:human_genes:E:ERG|ERG]]|-2.31|0.09| |[[:human_genes:T:TK1|TK1]]|-2.31|0.06| |[[:human_genes:A:ASUN|ASUN]]|-2.30|0.09| |[[:human_genes:V:VPS51|VPS51]]|-2.27|0.10| |[[:human_genes:N:NANS|NANS]]|2.77|<0.01| |[[:human_genes:A:ARID4A|ARID4A]]|2.80|<0.01| |[[:human_genes:C:CDC5L|CDC5L]]|2.88|<0.01| |[[:human_genes:N:NDUFB8|NDUFB8]]|2.89|<0.01| |[[:human_genes:N:NKTR|NKTR]]|2.89|0.02| |[[:human_genes:Z:ZBTB18|ZBTB18]]|2.96|<0.01| |[[:human_genes:K:KAT7|KAT7]]|2.96|<0.01| |[[:human_genes:Z:ZFAND3|ZFAND3]]|3.01|<0.01| |[[:human_genes:R:RBM26|RBM26]]|3.01|<0.01| |[[:human_genes:M:MAEA|MAEA]]|3.02|<0.01| |[[:human_genes:D:DAZAP1|DAZAP1]]|3.03|<0.01| |[[:human_genes:S:SIRT6|SIRT6]]|3.08|<0.01| |[[:human_genes:I:ITPR1|ITPR1]]|3.10|<0.01| |[[:human_genes:P:PLCG2|PLCG2]]|3.13|<0.01| |[[:human_genes:G:GID8|GID8]]|3.15|<0.01| |[[:human_genes:C:CXorf56|CXorf56]]|3.48|<0.01| |[[:human_genes:N:NFATC3|NFATC3]]|3.52|<0.01| |[[:human_genes:C:CNOT2|CNOT2]]|3.68|<0.01| |[[:human_genes:B:BTK|BTK]]|3.69|<0.01| |[[:human_genes:C:CDK8|CDK8]]|3.81|<0.01| |[[:human_genes:P:PPP3CA|PPP3CA]]|3.87|<0.01| |[[:human_genes:F:FAM122A|FAM122A]]|4.29|<0.01| |[[:human_genes:C:CNOT8|CNOT8]]|4.33|<0.01| |[[:human_genes:A:ARID4B|ARID4B]]|4.36|<0.01| |[[:human_genes:W:WDR26|WDR26]]|4.43|<0.01| |[[:human_genes:M:MEMO1|MEMO1]]|4.68|<0.01| |[[:human_genes:U:UBE2H|UBE2H]]|5.73|<0.01| |[[:human_genes:P:PPP3R1|PPP3R1]]|5.80|<0.01| |[[:human_genes:Y:YPEL5|YPEL5]]|6.73|<0.01| |[[:human_genes:F:FKBP1A|FKBP1A]]|8.08|<0.01| </modal> <modal id="modal_screen_similarity" size="lg" title="Correlated Screens"> ^Screen^Correlation^Plot^ |[[:results:exp29|Rapamycin 1μM R00 exp29]]|0.185|<button type="primary" size="small" modal="1">View</button>| |[[:results:exp306|Rapamycin 2μM R07 exp306]]|0.142|<button type="primary" size="small" modal="2">View</button>| |[[:results:exp307|Rapamycin 2μM plus Cyclosporin-A 3μM R07 exp307]]|0.1|<button type="primary" size="small" modal="3">View</button>| |[[:results:exp308|Rapamycin 2μM plus FK-506 5μM R07 exp308]]|0.089|<button type="primary" size="small" modal="4">View</button>| |[[:results:exp82|Torin1 0.08μM R02 exp82]]|0.056|<button type="primary" size="small" modal="5">View</button>| |[[:results:exp407|Thapsigargin 0.005μM R07 exp407]]|0.054|<button type="primary" size="small" modal="6">View</button>| |[[:results:exp535|Trimetrexate 0.03μM R08 exp535]]|0.054|<button type="primary" size="small" modal="7">View</button>| |[[:results:exp429|Rapamycin 0.001μM R08 exp429]]|0.148|<button type="primary" size="small" modal="8">View</button>| |[[:results:exp229|Dimethyloxaloylglycine 100μM R05 exp229]]|0.064|<button type="primary" size="small" modal="9">View</button>| |[[:results:exp488|Hippuristanol 0.12μM R08 exp488]]|0.063|<button type="primary" size="small" modal="10">View</button>| |[[:results:exp25|Oligomycin-A 2μM R00 exp25]]|0.062|<button type="primary" size="small" modal="11">View</button>| |[[:results:exp70|INK128 0.2μM R02 exp70]]|0.058|<button type="primary" size="small" modal="12">View</button>| |[[:results:exp504|MK2206 4μM R08 exp504]]|0.057|<button type="primary" size="small" modal="13">View</button>| |[[:results:exp497|Lead acetate 2000μM R08 exp497]]|0.057|<button type="primary" size="small" modal="14">View</button>| |[[:results:exp233|EPZ-5676 30μM R05 exp233]]|0.057|<button type="primary" size="small" modal="15">View</button>| |[[:results:exp498|Lead acetate 2000μM R08 exp498 no dilution day6]]|0.056|<button type="primary" size="small" modal="16">View</button>| |[[:results:exp234|Ethanol 0.01 R05 exp234]]|0.056|<button type="primary" size="small" modal="17">View</button>| |[[:results:exp216|Erlotinib 10μM R05 exp216]]|0.055|<button type="primary" size="small" modal="18">View</button>| |[[:results:exp485|GSK626616 14μM R08 exp485]]|0.054|<button type="primary" size="small" modal="19">View</button>| |[[:results:exp269|Bisphenol A 100μM R06 exp269]]|0.054|<button type="primary" size="small" modal="20">View</button>| |[[:results:exp317|Geldanamycin 0.015 to 0.05μM on day4 R07 exp317]]|0.054|<button type="primary" size="small" modal="21">View</button>| |[[:results:exp351|Dexamethasone 0.006μM R07 exp351]]|0.054|<button type="primary" size="small" modal="22">View</button>| |[[:results:exp489|Hippuristanol 0.12μM R08 exp489 no dilution day6]]|0.054|<button type="primary" size="small" modal="23">View</button>| |[[:results:exp449|Arsenic trioxide 60μM R08 exp449]]|0.051|<button type="primary" size="small" modal="24">View</button>| |[[:results:exp222|Betulinic acid 10 to 15μM on day4 R05 exp222]]|0.051|<button type="primary" size="small" modal="25">View</button>| </modal> <modal id="1" size="lg" title="Rapamycin 10μM R00 exp30 vs Rapamycin 1μM R00 exp29"> {{:chemogenomics:comparison_plots:exp29_vs_exp30.png?nolink |}} </modal> <modal id="2" size="lg" title="Rapamycin 10μM R00 exp30 vs Rapamycin 2μM R07 exp306"> {{:chemogenomics:comparison_plots:exp30_vs_exp306.png?nolink |}} </modal> <modal id="3" size="lg" title="Rapamycin 10μM R00 exp30 vs Rapamycin 2μM plus Cyclosporin-A 3μM R07 exp307"> {{:chemogenomics:comparison_plots:exp30_vs_exp307.png?nolink |}} </modal> <modal id="4" size="lg" title="Rapamycin 10μM R00 exp30 vs Rapamycin 2μM plus FK-506 5μM R07 exp308"> {{:chemogenomics:comparison_plots:exp30_vs_exp308.png?nolink |}} </modal> <modal id="5" size="lg" title="Rapamycin 10μM R00 exp30 vs Torin1 0.08μM R02 exp82"> {{:chemogenomics:comparison_plots:exp30_vs_exp82.png?nolink |}} </modal> <modal id="6" size="lg" title="Rapamycin 10μM R00 exp30 vs Thapsigargin 0.005μM R07 exp407"> {{:chemogenomics:comparison_plots:exp30_vs_exp407.png?nolink |}} </modal> <modal id="7" size="lg" title="Rapamycin 10μM R00 exp30 vs Trimetrexate 0.03μM R08 exp535"> {{:chemogenomics:comparison_plots:exp30_vs_exp535.png?nolink |}} </modal> <modal id="8" size="lg" title="Rapamycin 0.001μM R08 exp429 vs Rapamycin 10μM R00 exp30"> {{:chemogenomics:comparison_plots:exp30_vs_exp429.png?nolink |}} </modal> <modal id="9" size="lg" title="Dimethyloxaloylglycine 100μM R05 exp229 vs Rapamycin 10μM R00 exp30"> {{:chemogenomics:comparison_plots:exp229_vs_exp30.png?nolink |}} </modal> <modal id="10" size="lg" title="Hippuristanol 0.12μM R08 exp488 vs Rapamycin 10μM R00 exp30"> {{:chemogenomics:comparison_plots:exp30_vs_exp488.png?nolink |}} </modal> <modal id="11" size="lg" title="Oligomycin-A 2μM R00 exp25 vs Rapamycin 10μM R00 exp30"> {{:chemogenomics:comparison_plots:exp25_vs_exp30.png?nolink |}} </modal> <modal id="12" size="lg" title="INK128 0.2μM R02 exp70 vs Rapamycin 10μM R00 exp30"> {{:chemogenomics:comparison_plots:exp30_vs_exp70.png?nolink |}} </modal> <modal id="13" size="lg" title="MK2206 4μM R08 exp504 vs Rapamycin 10μM R00 exp30"> {{:chemogenomics:comparison_plots:exp30_vs_exp504.png?nolink |}} </modal> <modal id="14" size="lg" title="Lead acetate 2000μM R08 exp497 vs Rapamycin 10μM R00 exp30"> {{:chemogenomics:comparison_plots:exp30_vs_exp497.png?nolink |}} </modal> <modal id="15" size="lg" title="EPZ-5676 30μM R05 exp233 vs Rapamycin 10μM R00 exp30"> {{:chemogenomics:comparison_plots:exp233_vs_exp30.png?nolink |}} </modal> <modal id="16" size="lg" title="Lead acetate 2000μM R08 exp498 no dilution day6 vs Rapamycin 10μM R00 exp30"> {{:chemogenomics:comparison_plots:exp30_vs_exp498.png?nolink |}} </modal> <modal id="17" size="lg" title="Ethanol 0.01 R05 exp234 vs Rapamycin 10μM R00 exp30"> {{:chemogenomics:comparison_plots:exp234_vs_exp30.png?nolink |}} </modal> <modal id="18" size="lg" title="Erlotinib 10μM R05 exp216 vs Rapamycin 10μM R00 exp30"> {{:chemogenomics:comparison_plots:exp216_vs_exp30.png?nolink |}} </modal> <modal id="19" size="lg" title="GSK626616 14μM R08 exp485 vs Rapamycin 10μM R00 exp30"> {{:chemogenomics:comparison_plots:exp30_vs_exp485.png?nolink |}} </modal> <modal id="20" size="lg" title="Bisphenol A 100μM R06 exp269 vs Rapamycin 10μM R00 exp30"> {{:chemogenomics:comparison_plots:exp269_vs_exp30.png?nolink |}} </modal> <modal id="21" size="lg" title="Geldanamycin 0.015 to 0.05μM on day4 R07 exp317 vs Rapamycin 10μM R00 exp30"> {{:chemogenomics:comparison_plots:exp30_vs_exp317.png?nolink |}} </modal> <modal id="22" size="lg" title="Dexamethasone 0.006μM R07 exp351 vs Rapamycin 10μM R00 exp30"> {{:chemogenomics:comparison_plots:exp30_vs_exp351.png?nolink |}} </modal> <modal id="23" size="lg" title="Hippuristanol 0.12μM R08 exp489 no dilution day6 vs Rapamycin 10μM R00 exp30"> {{:chemogenomics:comparison_plots:exp30_vs_exp489.png?nolink |}} </modal> <modal id="24" size="lg" title="Arsenic trioxide 60μM R08 exp449 vs Rapamycin 10μM R00 exp30"> {{:chemogenomics:comparison_plots:exp30_vs_exp449.png?nolink |}} </modal> <modal id="25" size="lg" title="Betulinic acid 10 to 15μM on day4 R05 exp222 vs Rapamycin 10μM R00 exp30"> {{:chemogenomics:comparison_plots:exp222_vs_exp30.png?nolink |}} </modal> <modal id="modal_go_sensitive" size="lg" title="Top 30 sensitive GO terms">No GO term hits below threshold. (FDR <0.05)</modal> <modal id="modal_go_resistant" size="lg" title="Top 30 resistant GO terms"> ^GO Term^Fold Change^Genes^ |establishment of Sertoli cell barrier|368.47|ARID4B,ARID4A| |GID complex|276.35|WDR26,MAEA| |negative regulation of calcium ion import across plasma membrane|276.35|PPP3R1,PPP3CA| |calcineurin complex|221.08|PPP3CA,ITPR1| |calcium-dependent protein serine/threonine phosphatase activity|184.23|PPP3R1,PPP3CA| |calcineurin-NFAT signaling cascade|118.44|PPP3R1,PPP3CA,NFATC3| |cyclosporin A binding|103.63|PPP3R1,PPP3CA,NKTR| |calcineurin-mediated signaling|97.54|PPP3R1,PPP3CA,NFATC3| |positive regulation of calcium-mediated signaling|41.45|PPP3R1,PPP3CA,PLCG2| |negative regulation of calcium ion transmembrane transport|41.45|FKBP1A,PPP3R1,PPP3CA| |regulation of stem cell population maintenance|31.14|ARID4B,CNOT2,SIRT6,ARID4A| |regulation of calcium-mediated signaling|27.64|PPP3R1,PPP3CA,PLCG2,ITPR1| |calcium-mediated signaling|17.94|PPP3R1,PPP3CA,BTK,NFATC3,PLCG2| |intracellular protein-containing complex|6.31|YPEL5,PPP3R1,WDR26,CNOT8,PPP3CA,CNOT2,GID8,ITPR1,MAEA,KAT7| </modal> Last modified: 2026/01/15 21:36by 127.0.0.1