Rifampicin 1μM R00 exp31 [The Human Chemical-Genetic Interaction Map Project]

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====== Rifampicin 1μM R00 exp31 ======
==== Mechanism of Action ====
Inhibits bacterial RNA polymerase, induces hepatic cytochrome P450 enzymes
  * **<color #00a2e8>Class / Subclass 1:</color>** Infectious Disease / Antibiotic




==== Technical Notes ====
<accordion collapsed='true'>
<panel title='Compound References' collapsed='true'>
    * **<color #00a2e8>PubChem Name:</color>** %%Rifampicin%%
    * **<color #00a2e8>Synonyms:</color>** Rifampin; Rifamycin AMP
    * **<color #00a2e8>CAS #:</color>** 13292-46-1
    * **<color #00a2e8>PubChem CID:</color>** [[https://pubchem.ncbi.nlm.nih.gov/compound/135398735|135398735]]
    * **<color #00a2e8>IUPAC:</color>** %%[(7S,9E,11S,12R,13S,14R,15R,16R,17S,18S,19E,21Z)-2,15,17,27,29-pentahydroxy-11-methoxy-3,7,12,14,16,18,22-heptamethyl-26-[(E)-(4-methylpiperazin-1-yl)iminomethyl]-6,23-dioxo-8,30-dioxa-24-azatetracyclo[23.3.1.14,7.05,28]triaconta-1(29),2,4,9,19,21,25,27-octaen-13-yl] acetate%%
    * **<color #00a2e8>INCHI Name:</color>** InChI=1S/C43H58N4O12/c1-21-12-11-13-22(2)42(55)45-33-28(20-44-47-17-15-46(9)16-18-47)37(52)30-31(38(33)53)36(51)26(6)40-32(30)41(54)43(8,59-40)57-19-14-29(56-10)23(3)39(58-27(7)48)25(5)35(50)24(4)34(21)49/h11-14,19-21,23-25,29,34-35,39,49-53H,15-18H2,1-10H3,(H,45,55)/b12-11+,19-14+,22-13-,44-20+/t21-,23+,24+,25+,29-,34-,35+,39+,43-/m0/s1
    * **<color #00a2e8>INCHI Key:</color>** JQXXHWHPUNPDRT-WLSIYKJHSA-N
    * **<color #00a2e8>Molecular Weight:</color>** 822.9
    * **<color #00a2e8>Canonical SMILES:</color>** CC1C=CC=C(C(=O)NC2=C(C(=C3C(=C2O)C(=C(C4=C3C(=O)C(O4)(OC=CC(C(C(C(C(C(C1O)C)O)C)OC(=O)C)C)OC)C)C)O)O)C=NN5CCN(CC5)C)C
    * **<color #00a2e8>Isomeric SMILES:</color>** C[C@H]1/C=C/C=C(\\C(=O)NC2=C(C(=C3C(=C2O)C(=C(C4=C3C(=O)[C@](O4)(O/C=C/[C@@H]([C@H]([C@H]([C@@H]([C@@H]([C@@H]([C@H]1O)C)O)C)OC(=O)C)C)OC)C)C)O)O)/C=N/N5CCN(CC5)C)/C
    * **<color #00a2e8>Molecular Formula:</color>** C43H58N4O12
{{:chemogenomics:structures:chem-0017.svg?nolink}}
</panel>


<panel title='Compound Supplier' collapsed='true'>
    * **<color #00a2e8>Supplier Name:</color>** Med Chem Express
    * **<color #00a2e8>Catalog #:</color>** HY-B0272
    * **<color #00a2e8>Lot #:</color>** 33587
</panel>


<panel title='Compound Characterization' collapsed='true'>
    * **<color #00a2e8>HRMS (ESI-TOF) m/z:</color>** (M+H)+ Calcd for C43H58N4O12 823.4124; found 823.4128
</panel>


<panel title='Dose Response Curve' collapsed='true'>
    * **<color #00a2e8>Platform ID:</color>** Rifampicin
    * **<color #00a2e8>Min:</color>** -17.8934; **<color #00a2e8>Max:</color>** 5.5857
{{:chemogenomics:dose_response:dr_274.png?nolink&500 |}}
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|< 300px 100px 200px >|
^ IC   ^ Concentration (µM) ^
| IC10  |N/A    |
| IC20  |N/A    |
| IC30  |N/A    |
| IC40  |N/A    |
| IC50  |N/A    |
| IC60  |N/A    |
| IC70  |N/A    |
| IC80  |N/A    |
| IC90  |N/A    |
\\ 
</panel>
</accordion>
==== Screen Summary ====
    * **<color #00a2e8>Round</color>**: 00
    * **<color #00a2e8>Dose</color>**: 1µM
    * **<color #00a2e8>Days of incubation</color>**: 8
    * **<color #00a2e8>Doublings</color>**: 6.4
    * **<color #00a2e8>Numbers of reads</color>**: 11435445


==== Screen Results ====


^Sensitive/Resistant hits (FDR<0.05)^CRANKS^Score Plot^Top 30 Genes^Screen Similarity^Top 30 Sensitive GO terms^Top 30 Resistant GO terms^
|0/0|<color #e85500>[[https://files.tyerslab.com/files/public/chemogenomics/cranks/Rifampicin_1uM_Round-0_exp31.txt|Scores]]</color>|<button type="primary" size="sm" modal="ScorePlot">View</button>|<button type="primary" size="sm" modal="modal_hits">View</button>|<button type="primary" size="sm" modal="modal_screen_similarity">View</button>|<button type="default" size="sm" modal="modal_go_sensitive">View</button>|<button type="default" size="sm" modal="modal_go_resistant">View</button>|
<modal id='ScorePlot' size='lg' title='Rifampicin 1μM R00 exp31'>
{{:chemogenomics:cranks_plots:exp31.png?nolink}}
</modal>


<modal id="modal_hits" size="sm" title="Top 30 S/R Genes">
^Gene^CRANKS Score^FDR^
|[[:human_genes:A:ADK|ADK]]|-2.69|0.31|
|[[:human_genes:P:PRMT5|PRMT5]]|-2.57|0.49|
|[[:human_genes:X:XPO5|XPO5]]|-2.43|0.45|
|[[:human_genes:G:GGPS1|GGPS1]]|-2.36|0.45|
|[[:human_genes:A:AIRE|AIRE]]|-2.32|0.45|
|[[:human_genes:C:CDIPT|CDIPT]]|-2.30|0.45|
|[[:human_genes:G:GPBP1|GPBP1]]|-2.18|0.45|
|[[:human_genes:I:ITFG2|ITFG2]]|-2.15|0.45|
|[[:human_genes:M:MRPL41|MRPL41]]|-2.13|0.45|
|[[:human_genes:M:MIOS|MIOS]]|-2.11|0.45|
|[[:human_genes:B:BTNL9|BTNL9]]|-2.10|0.45|
|[[:human_genes:L:LRP10|LRP10]]|-2.10|0.45|
|[[:human_genes:P:PRAMEF25|PRAMEF25]]|-2.10|0.75|
|[[:human_genes:P:PRAMEF26|PRAMEF26]]|-2.07|0.75|
|[[:human_genes:L:LEO1|LEO1]]|-2.07|0.45|
|[[:human_genes:M:MEF2A|MEF2A]]|-2.05|0.45|
|[[:human_genes:S:SNAPC5|SNAPC5]]|-2.04|0.45|
|[[:human_genes:M:MME|MME]]|-2.03|0.45|
|[[:human_genes:S:SYNCRIP|SYNCRIP]]|-2.01|0.49|
|[[:human_genes:C:CALD1|CALD1]]|-2.01|0.49|
|[[:human_genes:R:RBPJL|RBPJL]]|-2.01|0.45|
|[[:human_genes:A:ASB3|ASB3]]|-2.00|0.45|
|[[:human_genes:C:CCDC47|CCDC47]]|-1.99|0.45|
|[[:human_genes:D:DUOX2|DUOX2]]|-1.99|0.45|
|[[:human_genes:F:FGFBP2|FGFBP2]]|-1.98|0.45|
|[[:human_genes:P:PFN1|PFN1]]|-1.98|0.45|
|[[:human_genes:G:GMPPB|GMPPB]]|-1.97|0.56|
|[[:human_genes:T:TMEM134|TMEM134]]|-1.95|0.45|
|[[:human_genes:G:GPR75-ASB3|GPR75-ASB3]]|-1.95|0.45|
|[[:human_genes:T:TEN1|TEN1]]|-1.92|0.45|
|[[:human_genes:A:APEX1|APEX1]]|2.08|0.35|
|[[:human_genes:K:KLHL31|KLHL31]]|2.09|0.35|
|[[:human_genes:P:PLK4|PLK4]]|2.10|0.55|
|[[:human_genes:S:SMG5|SMG5]]|2.11|0.62|
|[[:human_genes:S:SNRNP200|SNRNP200]]|2.12|0.49|
|[[:human_genes:C:CCT4|CCT4]]|2.12|0.62|
|[[:human_genes:S:SMU1|SMU1]]|2.13|0.54|
|[[:human_genes:E:ERCC6L2|ERCC6L2]]|2.17|0.27|
|[[:human_genes:H:HSPA5|HSPA5]]|2.18|0.56|
|[[:human_genes:P:POLE|POLE]]|2.19|0.56|
|[[:human_genes:M:MNT|MNT]]|2.19|0.45|
|[[:human_genes:P:PYHIN1|PYHIN1]]|2.20|0.26|
|[[:human_genes:S:SNRPF|SNRPF]]|2.21|0.56|
|[[:human_genes:O:OR2G3|OR2G3]]|2.21|0.26|
|[[:human_genes:A:AHCTF1|AHCTF1]]|2.22|0.46|
|[[:human_genes:S:SSSCA1|SSSCA1]]|2.22|0.26|
|[[:human_genes:R:RPS6|RPS6]]|2.27|0.55|
|[[:human_genes:A:ANKLE2|ANKLE2]]|2.28|0.45|
|[[:human_genes:T:TRAPPC2L|TRAPPC2L]]|2.29|0.26|
|[[:human_genes:T:TPX2|TPX2]]|2.30|0.49|
|[[:human_genes:H:HGF|HGF]]|2.31|0.36|
|[[:human_genes:Z:ZDBF2|ZDBF2]]|2.34|0.25|
|[[:human_genes:D:DONSON|DONSON]]|2.39|0.53|
|[[:human_genes:C:CDC45|CDC45]]|2.42|0.46|
|[[:human_genes:D:DHX15|DHX15]]|2.47|0.49|
|[[:human_genes:C:CPOX|CPOX]]|2.49|0.20|
|[[:human_genes:S:SLC25A32|SLC25A32]]|2.50|0.25|
|[[:human_genes:S:SUPT6H|SUPT6H]]|2.71|0.45|
|[[:human_genes:A:ALAS1|ALAS1]]|2.91|0.10|
|[[:human_genes:C:COPE|COPE]]|2.98|0.26|
</modal>


<modal id="modal_screen_similarity" size="lg" title="Correlated Screens">
^Screen^Correlation^Plot^
|[[:results:exp27|Pimelic-diphenylamide-106 0.5μM R00 exp27]]|0.084|<button type="primary" size="small" modal="1">View</button>|
|[[:results:exp15|Cycloheximide 0.2μM R00 exp15]]|0.071|<button type="primary" size="small" modal="2">View</button>|
|[[:results:exp12|Chloramphenicol 2μM R00 exp12]]|0.068|<button type="primary" size="small" modal="3">View</button>|
|[[:results:exp5|Alpha-Amanitin 0.5μM R00 exp5]]|0.062|<button type="primary" size="small" modal="4">View</button>|
|[[:results:exp10|CCCP 0.1μM R00 exp10]]|0.054|<button type="primary" size="small" modal="5">View</button>|
|[[:results:exp77|Prochlorperazine 5.2μM R02 exp77]]|0.053|<button type="primary" size="small" modal="6">View</button>|
</modal>


<modal id="1" size="lg" title="Pimelic-diphenylamide-106 0.5μM R00 exp27  vs  Rifampicin 1μM R00 exp31">
{{:chemogenomics:comparison_plots:exp27_vs_exp31.png?nolink |}}
</modal>

<modal id="2" size="lg" title="Cycloheximide 0.2μM R00 exp15  vs  Rifampicin 1μM R00 exp31">
{{:chemogenomics:comparison_plots:exp15_vs_exp31.png?nolink |}}
</modal>

<modal id="3" size="lg" title="Chloramphenicol 2μM R00 exp12  vs  Rifampicin 1μM R00 exp31">
{{:chemogenomics:comparison_plots:exp12_vs_exp31.png?nolink |}}
</modal>

<modal id="4" size="lg" title="Alpha-Amanitin 0.5μM R00 exp5  vs  Rifampicin 1μM R00 exp31">
{{:chemogenomics:comparison_plots:exp31_vs_exp5.png?nolink |}}
</modal>

<modal id="5" size="lg" title="CCCP 0.1μM R00 exp10  vs  Rifampicin 1μM R00 exp31">
{{:chemogenomics:comparison_plots:exp10_vs_exp31.png?nolink |}}
</modal>

<modal id="6" size="lg" title="Prochlorperazine 5.2μM R02 exp77  vs  Rifampicin 1μM R00 exp31">
{{:chemogenomics:comparison_plots:exp31_vs_exp77.png?nolink |}}
</modal>



<modal id="modal_go_sensitive" size="lg" title="Top 30 sensitive GO terms">No GO term hits below threshold. (FDR <0.05)</modal>
<modal id="modal_go_resistant" size="lg" title="Top 30 resistant GO terms">No GO term hits below threshold. (FDR <0.05)</modal>