CCT251545 20μM R07 exp326 [The Human Chemical-Genetic Interaction Map Project]

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====== CCT251545 20μM R07 exp326 ======
==== Mechanism of Action ====
Inhibits CDK8 and CDK19 mediator-associated kinases
  * **<color #00a2e8>Class / Subclass 1:</color>** Gene Regulation / Transcription Inhibitor
  * **<color #00a2e8>Class / Subclass 2:</color>** Signal Transduction / Kinase Inhibitor



==== Technical Notes ====
<accordion collapsed='true'>
<panel title='Compound References' collapsed='true'>
    * **<color #00a2e8>PubChem Name:</color>** %%8-{3-Chloro-5-[4-(1-Methyl-1h-Pyrazol-4-Yl)phenyl]pyridin-4-Yl}-2,8-Diazaspiro[4.5]decan-1-One%%
    * **<color #00a2e8>Synonyms:</color>** N/A
    * **<color #00a2e8>CAS #:</color>** 1661839-45-7
    * **<color #00a2e8>PubChem CID:</color>** [[https://pubchem.ncbi.nlm.nih.gov/compound/77050682|77050682]]
    * **<color #00a2e8>IUPAC:</color>** %%8-[3-chloro-5-[4-(1-methylpyrazol-4-yl)phenyl]pyridin-4-yl]-2,8-diazaspiro[4.5]decan-1-one%%
    * **<color #00a2e8>INCHI Name:</color>** InChI=1S/C23H24ClN5O/c1-28-15-18(12-27-28)16-2-4-17(5-3-16)19-13-25-14-20(24)21(19)29-10-7-23(8-11-29)6-9-26-22(23)30/h2-5,12-15H,6-11H2,1H3,(H,26,30)
    * **<color #00a2e8>INCHI Key:</color>** LBFYQISQYCGDDW-UHFFFAOYSA-N
    * **<color #00a2e8>Molecular Weight:</color>** 421.9
    * **<color #00a2e8>Canonical SMILES:</color>** CN1C=C(C=N1)C2=CC=C(C=C2)C3=CN=CC(=C3N4CCC5(CCNC5=O)CC4)Cl
    * **<color #00a2e8>Isomeric SMILES:</color>** N/A
    * **<color #00a2e8>Molecular Formula:</color>** C23H24ClN5O
{{:chemogenomics:structures:chem-0163.svg?nolink}}
</panel>


<panel title='Compound Supplier' collapsed='true'>
    * **<color #00a2e8>Supplier Name:</color>** Cayman Chemical
    * **<color #00a2e8>Catalog #:</color>** 18761
    * **<color #00a2e8>Lot #:</color>** N/A
</panel>


<panel title='Compound Characterization' collapsed='true'>
    * **<color #00a2e8>HRMS (ESI-TOF) m/z:</color>** (M+H)+ Calcd for C23H24ClN5O 422.17421; found 422.1743
</panel>


<panel title='Dose Response Curve' collapsed='true'>
    * **<color #00a2e8>Platform ID:</color>** CCT251545
    * **<color #00a2e8>Min:</color>** -8.9474; **<color #00a2e8>Max:</color>** 38.9173
{{:chemogenomics:dose_response:dr_377.png?nolink&500 |}}
\\ 
\\ 
\\ 
\\ 
|< 300px 100px 200px >|
^ IC   ^ Concentration (µM) ^
| IC10  |N/A    |
| IC20  |N/A    |
| IC30  |N/A    |
| IC40  |N/A    |
| IC50  |N/A    |
| IC60  |N/A    |
| IC70  |N/A    |
| IC80  |N/A    |
| IC90  |N/A    |
\\ 
</panel>
</accordion>
==== Screen Summary ====
    * **<color #00a2e8>Round</color>**: 07
    * **<color #00a2e8>Dose</color>**: 20µM
    * **<color #00a2e8>Days of incubation</color>**: 8
    * **<color #00a2e8>Doublings</color>**: 4.0
    * **<color #00a2e8>Numbers of reads</color>**: 22004748


==== Screen Results ====


^Sensitive/Resistant hits (FDR<0.05)^CRANKS^Score Plot^Top 30 Genes^Screen Similarity^Top 30 Sensitive GO terms^Top 30 Resistant GO terms^
|7/1|<color #e85500>[[https://files.tyerslab.com/files/public/chemogenomics/cranks/CCT251545_20uM_Round-7_exp326.txt|Scores]]</color>|<button type="primary" size="sm" modal="ScorePlot">View</button>|<button type="primary" size="sm" modal="modal_hits">View</button>|<button type="primary" size="sm" modal="modal_screen_similarity">View</button>|<button type="primary" size="sm" modal="modal_go_sensitive">View</button>|<button type="primary" size="sm" modal="modal_go_resistant">View</button>|
<modal id='ScorePlot' size='lg' title='CCT251545 20μM R07 exp326'>
{{:chemogenomics:cranks_plots:exp326.png?nolink}}
</modal>


<modal id="modal_hits" size="sm" title="Top 30 S/R Genes">
^Gene^CRANKS Score^FDR^
|[[:human_genes:S:SLC5A3|SLC5A3]]|-4.07|<0.01|
|[[:human_genes:V:VAC14|VAC14]]|-3.34|<0.01|
|[[:human_genes:K:KDSR|KDSR]]|-3.24|<0.01|
|[[:human_genes:P:PCYT1A|PCYT1A]]|-3.01|<0.01|
|[[:human_genes:C:CHKA|CHKA]]|-3.00|<0.01|
|[[:human_genes:A:ALG13|ALG13]]|-2.69|0.02|
|[[:human_genes:N:NPRL3|NPRL3]]|-2.66|0.09|
|[[:human_genes:A:AGPAT6|AGPAT6]]|-2.62|0.03|
|[[:human_genes:A:ARL1|ARL1]]|-2.45|0.06|
|[[:human_genes:L:LHPP|LHPP]]|-2.42|0.13|
|[[:human_genes:F:FLVCR1|FLVCR1]]|-2.39|0.08|
|[[:human_genes:K:KRI1|KRI1]]|-2.30|0.61|
|[[:human_genes:S:SWI5|SWI5]]|-2.27|0.18|
|[[:human_genes:G:GPR107|GPR107]]|-2.26|0.13|
|[[:human_genes:C:CCNB1|CCNB1]]|-2.25|0.18|
|[[:human_genes:U:UBN2|UBN2]]|-2.25|0.13|
|[[:human_genes:G:GCLC|GCLC]]|-2.24|0.13|
|[[:human_genes:C:CEPT1|CEPT1]]|-2.22|0.13|
|[[:human_genes:A:AGPAT1|AGPAT1]]|-2.17|0.35|
|[[:human_genes:N:NAT10|NAT10]]|-2.16|0.17|
|[[:human_genes:M:MRPS25|MRPS25]]|-2.12|0.28|
|[[:human_genes:C:COG5|COG5]]|-2.12|0.18|
|[[:human_genes:W:WDR20|WDR20]]|-2.09|0.21|
|[[:human_genes:A:ATG4D|ATG4D]]|-2.08|0.21|
|[[:human_genes:F:FAM129C|FAM129C]]|-2.07|0.21|
|[[:human_genes:A:ACSL4|ACSL4]]|-2.04|0.24|
|[[:human_genes:T:TIGD5|TIGD5]]|-2.02|0.35|
|[[:human_genes:S:SATB2|SATB2]]|-2.02|0.26|
|[[:human_genes:M:MS4A13|MS4A13]]|-2.01|0.77|
|[[:human_genes:M:MTG2|MTG2]]|-2.01|0.73|
|[[:human_genes:T:TRDN|TRDN]]|1.98|0.37|
|[[:human_genes:M:MBTPS2|MBTPS2]]|1.98|0.43|
|[[:human_genes:H:HIST1H2AI|HIST1H2AI]]|2.04|0.30|
|[[:human_genes:T:TMEM71|TMEM71]]|2.06|0.29|
|[[:human_genes:P:PRPF19|PRPF19]]|2.06|0.52|
|[[:human_genes:S:SEC13|SEC13]]|2.06|0.52|
|[[:human_genes:U:USP34|USP34]]|2.06|0.29|
|[[:human_genes:A:AMFR|AMFR]]|2.08|0.29|
|[[:human_genes:P:PUS3|PUS3]]|2.09|0.37|
|[[:human_genes:D:DDX10|DDX10]]|2.12|0.26|
|[[:human_genes:K:KDM3B|KDM3B]]|2.13|0.33|
|[[:human_genes:P:PCDHGA12|PCDHGA12]]|2.13|0.26|
|[[:human_genes:R:RALGAPB|RALGAPB]]|2.14|0.26|
|[[:human_genes:P:PLK4|PLK4]]|2.14|0.53|
|[[:human_genes:Z:ZNF431|ZNF431]]|2.16|0.41|
|[[:human_genes:A:AMBRA1|AMBRA1]]|2.24|0.26|
|[[:human_genes:M:MBD2|MBD2]]|2.24|0.26|
|[[:human_genes:G:GAPDHS|GAPDHS]]|2.25|0.26|
|[[:human_genes:B:BAP1|BAP1]]|2.29|0.32|
|[[:human_genes:R:RBCK1|RBCK1]]|2.32|0.26|
|[[:human_genes:N:NBPF1|NBPF1]]|2.35|0.26|
|[[:human_genes:E:EN1|EN1]]|2.37|0.22|
|[[:human_genes:P:POLD2|POLD2]]|2.38|0.46|
|[[:human_genes:S:SPI1|SPI1]]|2.41|0.26|
|[[:human_genes:M:MMS19|MMS19]]|2.44|0.26|
|[[:human_genes:P:POLE2|POLE2]]|2.46|0.43|
|[[:human_genes:R:RAB5C|RAB5C]]|2.54|0.10|
|[[:human_genes:R:RPL12|RPL12]]|2.56|0.27|
|[[:human_genes:L:LDB1|LDB1]]|2.68|0.06|
|[[:human_genes:Z:ZEB2|ZEB2]]|3.49|<0.01|
</modal>


<modal id="modal_screen_similarity" size="lg" title="Correlated Screens">
^Screen^Correlation^Plot^
|[[:results:exp149|SB203580 25μM R03 exp149]]|0.096|<button type="primary" size="small" modal="1">View</button>|
|[[:results:exp530|Thioridazine 5μM R08 exp530]]|0.085|<button type="primary" size="small" modal="2">View</button>|
|[[:results:exp422|Wiskostatin 3μM R07 exp422]]|0.071|<button type="primary" size="small" modal="3">View</button>|
|[[:results:exp344|Chlorpromazine 10μM R07 exp344]]|0.066|<button type="primary" size="small" modal="4">View</button>|
|[[:results:exp538|ZLN024 50μM R08 exp538]]|0.066|<button type="primary" size="small" modal="5">View</button>|
|[[:results:exp77|Prochlorperazine 5.2μM R02 exp77]]|0.066|<button type="primary" size="small" modal="6">View</button>|
|[[:results:exp358|FK-506 5μM R07 exp358]]|0.062|<button type="primary" size="small" modal="7">View</button>|
|[[:results:exp379|MSC2530818 10μM R07 exp379]]|0.061|<button type="primary" size="small" modal="8">View</button>|
|[[:results:exp465|Cannabidiol 13μM R08 exp465]]|0.061|<button type="primary" size="small" modal="9">View</button>|
|[[:results:exp433|LJH685 50μM R08 exp433]]|0.059|<button type="primary" size="small" modal="10">View</button>|
|[[:results:exp151|SGC0946 7μM R03 exp151]]|0.057|<button type="primary" size="small" modal="11">View</button>|
|[[:results:exp442|Ibrutinib 10μM R08 exp442]]|0.057|<button type="primary" size="small" modal="12">View</button>|
|[[:results:exp47|Lapatinib 5μM R01 exp47]]|0.054|<button type="primary" size="small" modal="13">View</button>|
|[[:results:exp233|EPZ-5676 30μM R05 exp233]]|0.052|<button type="primary" size="small" modal="14">View</button>|
|[[:results:exp510|Nicotine 3000μM R08 exp510]]|0.052|<button type="primary" size="small" modal="15">View</button>|
|[[:results:exp90|WYE-354 6μM R02 exp90]]|0.052|<button type="primary" size="small" modal="16">View</button>|
|[[:results:exp457|Bisphenol F 50μM R08 exp457]]|0.101|<button type="primary" size="small" modal="17">View</button>|
|[[:results:exp461|BS-181 20μM R08 exp461]]|0.074|<button type="primary" size="small" modal="18">View</button>|
|[[:results:exp169|BH1 1μM R04 exp169]]|0.058|<button type="primary" size="small" modal="19">View</button>|
|[[:results:exp341|BRD2 inhibitor II 20μM R07 exp341]]|0.053|<button type="primary" size="small" modal="20">View</button>|
</modal>


<modal id="1" size="lg" title="CCT251545 20μM R07 exp326  vs  SB203580 25μM R03 exp149">
{{:chemogenomics:comparison_plots:exp149_vs_exp326.png?nolink |}}
</modal>

<modal id="2" size="lg" title="CCT251545 20μM R07 exp326  vs  Thioridazine 5μM R08 exp530">
{{:chemogenomics:comparison_plots:exp326_vs_exp530.png?nolink |}}
</modal>

<modal id="3" size="lg" title="CCT251545 20μM R07 exp326  vs  Wiskostatin 3μM R07 exp422">
{{:chemogenomics:comparison_plots:exp326_vs_exp422.png?nolink |}}
</modal>

<modal id="4" size="lg" title="CCT251545 20μM R07 exp326  vs  Chlorpromazine 10μM R07 exp344">
{{:chemogenomics:comparison_plots:exp326_vs_exp344.png?nolink |}}
</modal>

<modal id="5" size="lg" title="CCT251545 20μM R07 exp326  vs  ZLN024 50μM R08 exp538">
{{:chemogenomics:comparison_plots:exp326_vs_exp538.png?nolink |}}
</modal>

<modal id="6" size="lg" title="CCT251545 20μM R07 exp326  vs  Prochlorperazine 5.2μM R02 exp77">
{{:chemogenomics:comparison_plots:exp326_vs_exp77.png?nolink |}}
</modal>

<modal id="7" size="lg" title="CCT251545 20μM R07 exp326  vs  FK-506 5μM R07 exp358">
{{:chemogenomics:comparison_plots:exp326_vs_exp358.png?nolink |}}
</modal>

<modal id="8" size="lg" title="CCT251545 20μM R07 exp326  vs  MSC2530818 10μM R07 exp379">
{{:chemogenomics:comparison_plots:exp326_vs_exp379.png?nolink |}}
</modal>

<modal id="9" size="lg" title="CCT251545 20μM R07 exp326  vs  Cannabidiol 13μM R08 exp465">
{{:chemogenomics:comparison_plots:exp326_vs_exp465.png?nolink |}}
</modal>

<modal id="10" size="lg" title="CCT251545 20μM R07 exp326  vs  LJH685 50μM R08 exp433">
{{:chemogenomics:comparison_plots:exp326_vs_exp433.png?nolink |}}
</modal>

<modal id="11" size="lg" title="CCT251545 20μM R07 exp326  vs  SGC0946 7μM R03 exp151">
{{:chemogenomics:comparison_plots:exp151_vs_exp326.png?nolink |}}
</modal>

<modal id="12" size="lg" title="CCT251545 20μM R07 exp326  vs  Ibrutinib 10μM R08 exp442">
{{:chemogenomics:comparison_plots:exp326_vs_exp442.png?nolink |}}
</modal>

<modal id="13" size="lg" title="CCT251545 20μM R07 exp326  vs  Lapatinib 5μM R01 exp47">
{{:chemogenomics:comparison_plots:exp326_vs_exp47.png?nolink |}}
</modal>

<modal id="14" size="lg" title="CCT251545 20μM R07 exp326  vs  EPZ-5676 30μM R05 exp233">
{{:chemogenomics:comparison_plots:exp233_vs_exp326.png?nolink |}}
</modal>

<modal id="15" size="lg" title="CCT251545 20μM R07 exp326  vs  Nicotine 3000μM R08 exp510">
{{:chemogenomics:comparison_plots:exp326_vs_exp510.png?nolink |}}
</modal>

<modal id="16" size="lg" title="CCT251545 20μM R07 exp326  vs  WYE-354 6μM R02 exp90">
{{:chemogenomics:comparison_plots:exp326_vs_exp90.png?nolink |}}
</modal>

<modal id="17" size="lg" title="Bisphenol F 50μM R08 exp457  vs  CCT251545 20μM R07 exp326">
{{:chemogenomics:comparison_plots:exp326_vs_exp457.png?nolink |}}
</modal>

<modal id="18" size="lg" title="BS-181 20μM R08 exp461  vs  CCT251545 20μM R07 exp326">
{{:chemogenomics:comparison_plots:exp326_vs_exp461.png?nolink |}}
</modal>

<modal id="19" size="lg" title="BH1 1μM R04 exp169  vs  CCT251545 20μM R07 exp326">
{{:chemogenomics:comparison_plots:exp169_vs_exp326.png?nolink |}}
</modal>

<modal id="20" size="lg" title="BRD2 inhibitor II 20μM R07 exp341  vs  CCT251545 20μM R07 exp326">
{{:chemogenomics:comparison_plots:exp326_vs_exp341.png?nolink |}}
</modal>



<modal id="modal_go_sensitive" size="lg" title="Top 30 sensitive GO terms">
^GO Term^Fold Change^Genes^
|CDP-choline pathway|345.40|PCYT1A,CHKA,CEPT1|
|phosphatidylcholine biosynthetic process|115.13|PCYT1A,CHKA,GPAT4,CEPT1|
|phosphatidylcholine metabolic process|40.63|PCYT1A,CHKA,GPAT4,CEPT1|
|glycerophospholipid biosynthetic process|21.48|VAC14,PCYT1A,CHKA,GPAT4,CEPT1,AGPAT1|
|glycerolipid biosynthetic process|19.55|VAC14,PCYT1A,CHKA,GPAT4,CEPT1,AGPAT1|
|phospholipid biosynthetic process|17.79|VAC14,PCYT1A,CHKA,GPAT4,CEPT1,AGPAT1|
|glycerophospholipid metabolic process|15.13|VAC14,PCYT1A,CHKA,GPAT4,CEPT1,AGPAT1|
|glycerolipid metabolic process|12.19|VAC14,PCYT1A,CHKA,GPAT4,CEPT1,AGPAT1|
|phospholipid metabolic process|11.98|VAC14,PCYT1A,CHKA,GPAT4,CEPT1,AGPAT1|
|organophosphate biosynthetic process|9.32|VAC14,PCYT1A,CHKA,GPAT4,CEPT1,AGPAT1,ACSL4|
|lipid biosynthetic process|8.44|VAC14,KDSR,PCYT1A,CHKA,GPAT4,AGPAT1,ACSL4|
|cellular lipid metabolic process|7.05|VAC14,KDSR,PCYT1A,CHKA,ALG13,GPAT4,CEPT1,AGPAT1,ACSL4|
</modal>
<modal id="modal_go_resistant" size="lg" title="Top 30 resistant GO terms">
^GO Term^Fold Change^Genes^
|CHD-type complex|110.53|MBD2,MTA2,GATAD2B|
|NuRD complex|110.53|MBD2,MTA2,GATAD2B|
|histone deacetylase complex|27.63|MBD2,KDM3B,MTA2,GATAD2B|
|chromatin DNA binding|24.03|BAP1,MBD2,KDM3B,MTA2,GATAD2B|
|chromatin binding|7.62|LDB1,SPI1,BAP1,MBD2,ZNF431,KDM3B,MTA2,GATAD2B|
</modal>