All-trans-Retinoic-Acid 8μM R07 exp333 [The Human Chemical-Genetic Interaction Map Project]

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====== All-trans-Retinoic-Acid 8μM R07 exp333 ======
==== Mechanism of Action ====
Ligand for retinoic acid receptor (RAR) and retinoid X receptor (RXR), metabolite of vitamin A1
  * **<color #00a2e8>Class / Subclass 1:</color>** Gene Regulation / Nuclear Receptor Ligand
  * **<color #00a2e8>Class / Subclass 2:</color>** Metabolism / Metabolite



==== Technical Notes ====
<accordion collapsed='true'>
<panel title='Compound References' collapsed='true'>
    * **<color #00a2e8>PubChem Name:</color>** %%Tretinoin%%
    * **<color #00a2e8>Synonyms:</color>** ATRA; Tretinoin; Vitamin A acid; all-trans-Retinoic acid; ATRA;Tretinoin;Vitamin A acid;all-trans-Retinoic acid
    * **<color #00a2e8>CAS #:</color>** 302-79-4
    * **<color #00a2e8>PubChem CID:</color>** [[https://pubchem.ncbi.nlm.nih.gov/compound/444795|444795]]
    * **<color #00a2e8>IUPAC:</color>** %%(2E,4E,6E,8E)-3,7-dimethyl-9-(2,6,6-trimethylcyclohexen-1-yl)nona-2,4,6,8-tetraenoic acid%%
    * **<color #00a2e8>INCHI Name:</color>** InChI=1S/C20H28O2/c1-15(8-6-9-16(2)14-19(21)22)11-12-18-17(3)10-7-13-20(18,4)5/h6,8-9,11-12,14H,7,10,13H2,1-5H3,(H,21,22)/b9-6+,12-11+,15-8+,16-14+
    * **<color #00a2e8>INCHI Key:</color>** SHGAZHPCJJPHSC-YCNIQYBTSA-N
    * **<color #00a2e8>Molecular Weight:</color>** 300.4
    * **<color #00a2e8>Canonical SMILES:</color>** CC1=C(C(CCC1)(C)C)C=CC(=CC=CC(=CC(=O)O)C)C
    * **<color #00a2e8>Isomeric SMILES:</color>** CC1=C(C(CCC1)(C)C)/C=C/C(=C/C=C/C(=C/C(=O)O)/C)/C
    * **<color #00a2e8>Molecular Formula:</color>** C20H28O2
{{:chemogenomics:structures:chem-0192.svg?nolink}}
</panel>


<panel title='Compound Supplier' collapsed='true'>
    * **<color #00a2e8>Supplier Name:</color>** Sigma-Aldrich
    * **<color #00a2e8>Catalog #:</color>** R2625
    * **<color #00a2e8>Lot #:</color>** SLBB5473V
</panel>


<panel title='Compound Characterization' collapsed='true'>
    * **<color #00a2e8>HRMS (ESI-TOF) m/z:</color>** (M+H)+ Calcd for C20H28O2 301.21621; found 301.21722
</panel>


<panel title='Dose Response Curve' collapsed='true'>
    * **<color #00a2e8>Platform ID:</color>** ATRA
    * **<color #00a2e8>Min:</color>** 8.9864; **<color #00a2e8>Max:</color>** 98.8174
{{:chemogenomics:dose_response:dr_488.png?nolink&500 |}}
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|< 300px 100px 200px >|
^ IC   ^ Concentration (µM) ^
| IC10  |N/A    |
| IC20  |0.0445    |
| IC30  |0.8254    |
| IC40  |6.3558    |
| IC50  |36.2335    |
| IC60  |N/A    |
| IC70  |N/A    |
| IC80  |N/A    |
| IC90  |N/A    |
\\ 
</panel>
</accordion>
==== Screen Summary ====
    * **<color #00a2e8>Round</color>**: 07
    * **<color #00a2e8>Dose</color>**: 8µM
    * **<color #00a2e8>Days of incubation</color>**: 8
    * **<color #00a2e8>Doublings</color>**: 6.5
    * **<color #00a2e8>Numbers of reads</color>**: 22540106


==== Screen Results ====


^Sensitive/Resistant hits (FDR<0.05)^CRANKS^Score Plot^Top 30 Genes^Screen Similarity^Top 30 Sensitive GO terms^Top 30 Resistant GO terms^
|0/0|<color #e85500>[[https://files.tyerslab.com/files/public/chemogenomics/cranks/All-trans-Retinoic-Acid_8uM_Round-7_exp333.txt|Scores]]</color>|<button type="primary" size="sm" modal="ScorePlot">View</button>|<button type="primary" size="sm" modal="modal_hits">View</button>|<button type="primary" size="sm" modal="modal_screen_similarity">View</button>|<button type="default" size="sm" modal="modal_go_sensitive">View</button>|<button type="default" size="sm" modal="modal_go_resistant">View</button>|
<modal id='ScorePlot' size='lg' title='All-trans-Retinoic-Acid 8μM R07 exp333'>
{{:chemogenomics:cranks_plots:exp333.png?nolink}}
</modal>


<modal id="modal_hits" size="sm" title="Top 30 S/R Genes">
^Gene^CRANKS Score^FDR^
|[[:human_genes:G:GNA13|GNA13]]|-2.69|0.07|
|[[:human_genes:R:RHOA|RHOA]]|-2.65|0.07|
|[[:human_genes:P:PGGT1B|PGGT1B]]|-2.56|0.08|
|[[:human_genes:Z:ZBTB10|ZBTB10]]|-2.53|0.08|
|[[:human_genes:S:SCAF8|SCAF8]]|-2.47|0.08|
|[[:human_genes:S:SEC61A2|SEC61A2]]|-2.40|0.11|
|[[:human_genes:U:UBE2A|UBE2A]]|-2.38|0.11|
|[[:human_genes:D:DDRGK1|DDRGK1]]|-2.30|0.23|
|[[:human_genes:C:CREBBP|CREBBP]]|-2.29|0.23|
|[[:human_genes:D:DERL2|DERL2]]|-2.27|0.18|
|[[:human_genes:C:CPPED1|CPPED1]]|-2.26|0.23|
|[[:human_genes:P:PRKAG1|PRKAG1]]|-2.21|0.21|
|[[:human_genes:A:ALG6|ALG6]]|-2.21|0.21|
|[[:human_genes:Z:ZFY|ZFY]]|-2.13|0.23|
|[[:human_genes:A:ABCB7|ABCB7]]|-2.10|0.24|
|[[:human_genes:S:SPEN|SPEN]]|-2.10|0.24|
|[[:human_genes:X:XPO5|XPO5]]|-2.07|0.34|
|[[:human_genes:R:RTCB|RTCB]]|-2.07|0.34|
|[[:human_genes:P:PSMA2|PSMA2]]|-2.06|0.51|
|[[:human_genes:D:DNAJC3|DNAJC3]]|-2.05|0.28|
|[[:human_genes:F:FAM219A|FAM219A]]|-2.05|0.28|
|[[:human_genes:C:CDC42BPA|CDC42BPA]]|-2.04|0.28|
|[[:human_genes:W:WARS|WARS]]|-2.04|0.35|
|[[:human_genes:A:ASIC2|ASIC2]]|-2.04|0.28|
|[[:human_genes:T:TCF4|TCF4]]|-2.03|0.28|
|[[:human_genes:S:SYCE2|SYCE2]]|-1.99|0.33|
|[[:human_genes:S:SHOX2|SHOX2]]|-1.99|0.49|
|[[:human_genes:Z:ZBTB7A|ZBTB7A]]|-1.98|0.33|
|[[:human_genes:T:TRIAP1|TRIAP1]]|-1.98|0.41|
|[[:human_genes:S:STAG2|STAG2]]|-1.96|0.34|
|[[:human_genes:S:SCAP|SCAP]]|1.90|0.59|
|[[:human_genes:A:ADO|ADO]]|1.93|0.59|
|[[:human_genes:N:NDRG1|NDRG1]]|1.94|0.59|
|[[:human_genes:O:OR8U8|OR8U8]]|1.94|0.64|
|[[:human_genes:R:RBM39|RBM39]]|1.94|0.66|
|[[:human_genes:C:CDK8|CDK8]]|1.95|0.59|
|[[:human_genes:Z:ZP1|ZP1]]|1.96|0.59|
|[[:human_genes:T:TRIM52|TRIM52]]|1.97|0.64|
|[[:human_genes:P:POLA2|POLA2]]|1.99|0.64|
|[[:human_genes:T:TMSB15B|TMSB15B]]|2.03|0.65|
|[[:human_genes:K:KIAA1211|KIAA1211]]|2.07|0.64|
|[[:human_genes:D:DPY19L2|DPY19L2]]|2.11|0.56|
|[[:human_genes:C:C4orf17|C4orf17]]|2.11|0.35|
|[[:human_genes:I:IFT74|IFT74]]|2.16|0.59|
|[[:human_genes:R:RPL11|RPL11]]|2.17|0.64|
|[[:human_genes:H:HOXA13|HOXA13]]|2.21|0.35|
|[[:human_genes:N:NAPA|NAPA]]|2.23|0.58|
|[[:human_genes:C:CCNB2|CCNB2]]|2.24|0.35|
|[[:human_genes:L:LTBP4|LTBP4]]|2.25|0.56|
|[[:human_genes:P:PSMB4|PSMB4]]|2.27|0.35|
|[[:human_genes:I:IQCK|IQCK]]|2.29|0.35|
|[[:human_genes:R:RCAN3|RCAN3]]|2.30|0.35|
|[[:human_genes:F:FAM200B|FAM200B]]|2.33|0.35|
|[[:human_genes:Z:ZNF217|ZNF217]]|2.33|0.35|
|[[:human_genes:C:CEP192|CEP192]]|2.33|0.44|
|[[:human_genes:R:RANGAP1|RANGAP1]]|2.41|0.35|
|[[:human_genes:N:NPIPB8|NPIPB8]]|2.53|0.59|
|[[:human_genes:P:PSMD3|PSMD3]]|2.56|0.59|
|[[:human_genes:P:PSMA7|PSMA7]]|2.62|0.35|
|[[:human_genes:S:SLC6A17|SLC6A17]]|2.69|0.34|
</modal>


<modal id="modal_screen_similarity" size="lg" title="Correlated Screens">
^Screen^Correlation^Plot^
|[[:results:exp347|Cyclosporin-A 0.8μM R07 exp347]]|0.063|<button type="primary" size="small" modal="1">View</button>|
|[[:results:exp316|Geldanamycin 0.015 to 0.025μM on day4 R07 exp316]]|0.053|<button type="primary" size="small" modal="2">View</button>|
</modal>


<modal id="1" size="lg" title="All-trans-Retinoic-Acid 8μM R07 exp333  vs  Cyclosporin-A 0.8μM R07 exp347">
{{:chemogenomics:comparison_plots:exp333_vs_exp347.png?nolink |}}
</modal>

<modal id="2" size="lg" title="All-trans-Retinoic-Acid 8μM R07 exp333  vs  Geldanamycin 0.015 to 0.025μM on day4 R07 exp316">
{{:chemogenomics:comparison_plots:exp316_vs_exp333.png?nolink |}}
</modal>



<modal id="modal_go_sensitive" size="lg" title="Top 30 sensitive GO terms">No GO term hits below threshold. (FDR <0.05)</modal>
<modal id="modal_go_resistant" size="lg" title="Top 30 resistant GO terms">No GO term hits below threshold. (FDR <0.05)</modal>