Cytochalasin-B 5μM R07 exp349 [The Human Chemical-Genetic Interaction Map Project]

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====== Cytochalasin-B 5μM R07 exp349 ======
==== Mechanism of Action ====
Binds fast-growing (barbed) end of F-actin filament, inhibits actin polymerization
  * **<color #00a2e8>Class / Subclass 1:</color>** Cell Cycle / Cytokinesis Inhibitor
  * **<color #00a2e8>Class / Subclass 2:</color>** Organelle Function / Cytoskeletal Inhibitor
  * **<color #00a2e8>Class / Subclass 3:</color>** Environmental Stresses / Toxin


==== Technical Notes ====
<accordion collapsed='true'>
<panel title='Compound References' collapsed='true'>
    * **<color #00a2e8>PubChem Name:</color>** %%Cytochalasin B%%
    * **<color #00a2e8>Synonyms:</color>** Phomin
    * **<color #00a2e8>CAS #:</color>** 14930-96-2
    * **<color #00a2e8>PubChem CID:</color>** [[https://pubchem.ncbi.nlm.nih.gov/compound/5311281|5311281]]
    * **<color #00a2e8>IUPAC:</color>** %%(1S,4E,6R,10R,12E,14S,15S,17S,18S,19S)-19-benzyl-6,15-dihydroxy-10,17-dimethyl-16-methylidene-2-oxa-20-azatricyclo[12.7.0.01,18]henicosa-4,12-diene-3,21-dione%%
    * **<color #00a2e8>INCHI Name:</color>** InChI=1S/C29H37NO5/c1-18-9-7-13-22(31)15-16-25(32)35-29-23(14-8-10-18)27(33)20(3)19(2)26(29)24(30-28(29)34)17-21-11-5-4-6-12-21/h4-6,8,11-12,14-16,18-19,22-24,26-27,31,33H,3,7,9-10,13,17H2,1-2H3,(H,30,34)/b14-8+,16-15+/t18-,19-,22-,23+,24+,26+,27-,29-/m1/s1
    * **<color #00a2e8>INCHI Key:</color>** GBOGMAARMMDZGR-TYHYBEHESA-N
    * **<color #00a2e8>Molecular Weight:</color>** 479.6
    * **<color #00a2e8>Canonical SMILES:</color>** CC1CCCC(C=CC(=O)OC23C(C=CC1)C(C(=C)C(C2C(NC3=O)CC4=CC=CC=C4)C)O)O
    * **<color #00a2e8>Isomeric SMILES:</color>** C[C@@H]1CCC[C@H](/C=C/C(=O)O[C@]23[C@@H](/C=C/C1)[C@@H](C(=C)[C@H]([C@H]2[C@@H](NC3=O)CC4=CC=CC=C4)C)O)O
    * **<color #00a2e8>Molecular Formula:</color>** C29H37NO5
{{:chemogenomics:structures:chem-0206.svg?nolink}}
</panel>


<panel title='Compound Supplier' collapsed='true'>
    * **<color #00a2e8>Supplier Name:</color>** Sigma-Aldrich
    * **<color #00a2e8>Catalog #:</color>** C6762
    * **<color #00a2e8>Lot #:</color>** 086M4129V
</panel>


<panel title='Compound Characterization' collapsed='true'>
    * **<color #00a2e8>HRMS (ESI-TOF) m/z:</color>** (M+H)+ Calcd for C29H37NO5 480.27445; found 480.27738
</panel>


<panel title='Dose Response Curve' collapsed='true'>
    * **<color #00a2e8>Platform ID:</color>** Cytochalasin_B
    * **<color #00a2e8>Min:</color>** -24.8534; **<color #00a2e8>Max:</color>** 80.8909
{{:chemogenomics:dose_response:dr_379.png?nolink&500 |}}
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|< 300px 100px 200px >|
^ IC   ^ Concentration (µM) ^
| IC10  |N/A    |
| IC20  |0.6851    |
| IC30  |1.3466    |
| IC40  |2.6729    |
| IC50  |5.6719    |
| IC60  |N/A    |
| IC70  |N/A    |
| IC80  |N/A    |
| IC90  |N/A    |
\\ 
</panel>
</accordion>
==== Screen Summary ====
    * **<color #00a2e8>Round</color>**: 07
    * **<color #00a2e8>Dose</color>**: 5µM
    * **<color #00a2e8>Days of incubation</color>**: 8
    * **<color #00a2e8>Doublings</color>**: -0.1
    * **<color #00a2e8>Numbers of reads</color>**: 21844473


==== Screen Results ====


^Sensitive/Resistant hits (FDR<0.05)^CRANKS^Score Plot^Top 30 Genes^Screen Similarity^Top 30 Sensitive GO terms^Top 30 Resistant GO terms^
|0/71|<color #e85500>[[https://files.tyerslab.com/files/public/chemogenomics/cranks/Cytochalasin_B_5uM_Round-7_exp349.txt|Scores]]</color>|<button type="primary" size="sm" modal="ScorePlot">View</button>|<button type="primary" size="sm" modal="modal_hits">View</button>|<button type="primary" size="sm" modal="modal_screen_similarity">View</button>|<button type="default" size="sm" modal="modal_go_sensitive">View</button>|<button type="primary" size="sm" modal="modal_go_resistant">View</button>|
<modal id='ScorePlot' size='lg' title='Cytochalasin-B 5μM R07 exp349'>
{{:chemogenomics:cranks_plots:exp349.png?nolink}}
</modal>


<modal id="modal_hits" size="sm" title="Top 30 S/R Genes">
^Gene^CRANKS Score^FDR^
|[[:human_genes:U:USP5|USP5]]|-2.75|0.08|
|[[:human_genes:Z:ZNF300|ZNF300]]|-2.66|0.08|
|[[:human_genes:V:VDAC1|VDAC1]]|-2.58|0.08|
|[[:human_genes:N:NHP2L1|NHP2L1]]|-2.58|0.40|
|[[:human_genes:N:NFS1|NFS1]]|-2.51|0.42|
|[[:human_genes:A:ATP6V0B|ATP6V0B]]|-2.43|0.33|
|[[:human_genes:A:ABCD1|ABCD1]]|-2.39|0.18|
|[[:human_genes:A:ARAP2|ARAP2]]|-2.34|0.18|
|[[:human_genes:F:FAM89B|FAM89B]]|-2.29|0.18|
|[[:human_genes:Z:ZBTB1|ZBTB1]]|-2.28|0.18|
|[[:human_genes:R:RCOR1|RCOR1]]|-2.28|0.18|
|[[:human_genes:V:VPS25|VPS25]]|-2.24|0.45|
|[[:human_genes:P:PSMA2|PSMA2]]|-2.23|0.40|
|[[:human_genes:D:DDX3X|DDX3X]]|-2.21|0.24|
|[[:human_genes:K:KPNB1|KPNB1]]|-2.18|0.60|
|[[:human_genes:C:CDC16|CDC16]]|-2.17|0.31|
|[[:human_genes:P:PPP2R2A|PPP2R2A]]|-2.15|0.24|
|[[:human_genes:P:PRPF4|PRPF4]]|-2.15|0.61|
|[[:human_genes:R:RSPH4A|RSPH4A]]|-2.15|0.40|
|[[:human_genes:N:NEK11|NEK11]]|-2.15|0.32|
|[[:human_genes:G:GATAD2A|GATAD2A]]|-2.15|0.24|
|[[:human_genes:Z:ZNF816-ZNF321P|ZNF816-ZNF321P]]|-2.14|0.24|
|[[:human_genes:C:CCM2|CCM2]]|-2.14|0.24|
|[[:human_genes:H:HSPA9|HSPA9]]|-2.13|0.69|
|[[:human_genes:S:SATB2|SATB2]]|-2.13|0.24|
|[[:human_genes:J:JAM2|JAM2]]|-2.10|0.34|
|[[:human_genes:P:POLR3G|POLR3G]]|-2.08|0.36|
|[[:human_genes:N:NDUFC2|NDUFC2]]|-2.08|0.30|
|[[:human_genes:R:R3HDM1|R3HDM1]]|-2.07|0.30|
|[[:human_genes:C:CDK5RAP2|CDK5RAP2]]|-2.06|0.36|
|[[:human_genes:R:RTTN|RTTN]]|3.21|0.00|
|[[:human_genes:R:RBM26|RBM26]]|3.32|0.00|
|[[:human_genes:B:BAK1|BAK1]]|3.35|0.00|
|[[:human_genes:E:ELAVL1|ELAVL1]]|3.37|0.00|
|[[:human_genes:A:ATMIN|ATMIN]]|3.40|0.00|
|[[:human_genes:T:TUBE1|TUBE1]]|3.41|0.00|
|[[:human_genes:E:EIF4A1|EIF4A1]]|3.52|0.00|
|[[:human_genes:C:CEP78|CEP78]]|3.75|0.00|
|[[:human_genes:C:CNTRL|CNTRL]]|3.92|0.00|
|[[:human_genes:F:FOPNL|FOPNL]]|3.93|0.00|
|[[:human_genes:C:CEP44|CEP44]]|3.93|0.00|
|[[:human_genes:B:BUB3|BUB3]]|3.95|0.00|
|[[:human_genes:C:C16orf59|C16orf59]]|4.16|0.00|
|[[:human_genes:S:SCLT1|SCLT1]]|4.37|0.00|
|[[:human_genes:N:NIN|NIN]]|4.51|0.00|
|[[:human_genes:A:ANKRD26|ANKRD26]]|4.56|0.00|
|[[:human_genes:C:CEP120|CEP120]]|4.57|0.00|
|[[:human_genes:C:CEP76|CEP76]]|4.65|0.00|
|[[:human_genes:T:TUBD1|TUBD1]]|4.88|0.00|
|[[:human_genes:C:CRADD|CRADD]]|4.98|0.00|
|[[:human_genes:C:C14orf80|C14orf80]]|5.15|0.00|
|[[:human_genes:O:ODF2|ODF2]]|5.23|0.00|
|[[:human_genes:C:CEP128|CEP128]]|5.32|0.00|
|[[:human_genes:P:PIDD|PIDD]]|5.81|0.00|
|[[:human_genes:C:CASP2|CASP2]]|6.29|0.00|
|[[:human_genes:O:OFD1|OFD1]]|6.31|0.00|
|[[:human_genes:P:PIBF1|PIBF1]]|6.48|0.00|
|[[:human_genes:C:CCDC41|CCDC41]]|6.58|0.00|
|[[:human_genes:C:C2CD3|C2CD3]]|6.67|0.00|
|[[:human_genes:K:KIAA0753|KIAA0753]]|6.75|0.00|
</modal>


<modal id="modal_screen_similarity" size="lg" title="Correlated Screens">
^Screen^Correlation^Plot^
|[[:results:exp374|Latrunculin-B 10μM R07 exp374]]|0.273|<button type="primary" size="small" modal="1">View</button>|
|[[:results:exp414|Tozasertib 0.1μM R07 exp414]]|0.223|<button type="primary" size="small" modal="2">View</button>|
|[[:results:exp80|RO-3307 4.7μM R02 exp80]]|0.118|<button type="primary" size="small" modal="3">View</button>|
|[[:results:exp512|Olaparib 4μM R08 exp512]]|0.066|<button type="primary" size="small" modal="4">View</button>|
|[[:results:exp485|GSK626616 14μM R08 exp485]]|0.061|<button type="primary" size="small" modal="5">View</button>|
|[[:results:exp488|Hippuristanol 0.12μM R08 exp488]]|0.055|<button type="primary" size="small" modal="6">View</button>|
|[[:results:exp524|Staurosporine 0.02μM R08 exp524]]|0.052|<button type="primary" size="small" modal="7">View</button>|
|[[:results:exp343|Centrinone 0.5μM R07 exp343]]|0.072|<button type="primary" size="small" modal="8">View</button>|
|[[:results:exp214|2-Deoxy-D-glucose 800μM R05 exp214]]|0.055|<button type="primary" size="small" modal="9">View</button>|
</modal>


<modal id="1" size="lg" title="Cytochalasin-B 5μM R07 exp349  vs  Latrunculin-B 10μM R07 exp374">
{{:chemogenomics:comparison_plots:exp349_vs_exp374.png?nolink |}}
</modal>

<modal id="2" size="lg" title="Cytochalasin-B 5μM R07 exp349  vs  Tozasertib 0.1μM R07 exp414">
{{:chemogenomics:comparison_plots:exp349_vs_exp414.png?nolink |}}
</modal>

<modal id="3" size="lg" title="Cytochalasin-B 5μM R07 exp349  vs  RO-3307 4.7μM R02 exp80">
{{:chemogenomics:comparison_plots:exp349_vs_exp80.png?nolink |}}
</modal>

<modal id="4" size="lg" title="Cytochalasin-B 5μM R07 exp349  vs  Olaparib 4μM R08 exp512">
{{:chemogenomics:comparison_plots:exp349_vs_exp512.png?nolink |}}
</modal>

<modal id="5" size="lg" title="Cytochalasin-B 5μM R07 exp349  vs  GSK626616 14μM R08 exp485">
{{:chemogenomics:comparison_plots:exp349_vs_exp485.png?nolink |}}
</modal>

<modal id="6" size="lg" title="Cytochalasin-B 5μM R07 exp349  vs  Hippuristanol 0.12μM R08 exp488">
{{:chemogenomics:comparison_plots:exp349_vs_exp488.png?nolink |}}
</modal>

<modal id="7" size="lg" title="Cytochalasin-B 5μM R07 exp349  vs  Staurosporine 0.02μM R08 exp524">
{{:chemogenomics:comparison_plots:exp349_vs_exp524.png?nolink |}}
</modal>

<modal id="8" size="lg" title="Centrinone 0.5μM R07 exp343  vs  Cytochalasin-B 5μM R07 exp349">
{{:chemogenomics:comparison_plots:exp343_vs_exp349.png?nolink |}}
</modal>

<modal id="9" size="lg" title="2-Deoxy-D-glucose 800μM R05 exp214  vs  Cytochalasin-B 5μM R07 exp349">
{{:chemogenomics:comparison_plots:exp214_vs_exp349.png?nolink |}}
</modal>



<modal id="modal_go_sensitive" size="lg" title="Top 30 sensitive GO terms">No GO term hits below threshold. (FDR <0.05)</modal>
<modal id="modal_go_resistant" size="lg" title="Top 30 resistant GO terms">
^GO Term^Fold Change^Genes^
|ciliary transition fiber|236.84|CEP83,NIN,SCLT1|
|centriole-centriole cohesion|184.21|ODF2,NIN,CEP44,RTTN|
|centriole replication|115.13|KIAA0753,C2CD3,OFD1,CEP44,RTTN|
|protein localization to centrosome|100.48|KIAA0753,C2CD3,CEP83,PIBF1|
|centriole assembly|89.13|KIAA0753,C2CD3,OFD1,CEP44,RTTN|
|centrosome duplication|78.95|KIAA0753,C2CD3,OFD1,CEP44,RTTN|
|centriole|56.39|KIAA0753,C2CD3,CEP83,OFD1,CEP128,ODF2,TUBD1,CEP76,CEP120,NIN,SCLT1,CEP44,CEP20,CEP78,RTTN|
|centrosome cycle|55.26|KIAA0753,C2CD3,OFD1,ODF2,CEP120,NIN,CEP44,TUBE1,RTTN|
|microtubule organizing center organization|46.48|KIAA0753,C2CD3,OFD1,ODF2,CEP120,NIN,CEP44,TUBE1,RTTN|
|centriolar satellite|29.09|KIAA0753,C2CD3,PIBF1,OFD1,CEP20,CNTRL|
|cilium organization|18.82|KIAA0753,C2CD3,CEP83,PIBF1,OFD1,ODF2,SCLT1,CEP20,CEP78,ATMIN,RTTN|
|centrosome|16.20|KIAA0753,C2CD3,CEP83,PIBF1,OFD1,CEP128,ODF2,CEP76,CEP120,ANKRD26,NIN,SCLT1,CEP44,CEP20,CNTRL,CEP78,TUBE1,RTTN|
|cilium assembly|14.99|KIAA0753,C2CD3,CEP83,PIBF1,OFD1,SCLT1,CEP20,ATMIN|
|microtubule organizing center|13.04|KIAA0753,C2CD3,CEP83,PIBF1,OFD1,CEP128,ODF2,TUBD1,CEP76,CEP120,ANKRD26,NIN,SCLT1,CEP44,CEP20,CNTRL,CEP78,TUBE1,RTTN|
|microtubule cytoskeleton organization|12.33|KIAA0753,C2CD3,PIBF1,OFD1,ODF2,TUBD1,CEP120,NIN,CEP44,CEP20,TUBE1,RTTN|
|plasma membrane bounded cell projection assembly|11.19|KIAA0753,C2CD3,CEP83,PIBF1,OFD1,SCLT1,CEP20,ATMIN|
|cilium|9.77|C2CD3,CEP83,OFD1,CEP128,ODF2,TUBD1,NIN,SCLT1,CEP20,CNTRL,CEP78,RTTN|
|cell cycle process|8.87|KIAA0753,C2CD3,PIBF1,OFD1,CASP2,PIDD1,ODF2,CRADD,CEP120,NIN,BUB3,CEP44,TUBE1,RTTN|
|microtubule-based process|8.51|KIAA0753,C2CD3,PIBF1,OFD1,ODF2,TUBD1,CEP120,NIN,CEP44,CEP20,TUBE1,RTTN|
</modal>