Genistein 15μM R07 exp360 [The Human Chemical-Genetic Interaction Map Project]

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====== Genistein 15μM R07 exp360 ======
==== Mechanism of Action ====
Inhibits tyrosine kinases, phytoestrogen, toposiomerase II, pleiotropic effects
  * **<color #00a2e8>Class / Subclass 1:</color>** Signal Transduction / Kinase Inhibitor
  * **<color #00a2e8>Class / Subclass 2:</color>** Metabolism / Hormone Agonist
  * **<color #00a2e8>Class / Subclass 3:</color>** Gene Regulation / Nuclear Receptor Ligand


==== Technical Notes ====
<accordion collapsed='true'>
<panel title='Compound References' collapsed='true'>
    * **<color #00a2e8>PubChem Name:</color>** %%Genistein%%
    * **<color #00a2e8>Synonyms:</color>** NPI 031L
    * **<color #00a2e8>CAS #:</color>** 446-72-0
    * **<color #00a2e8>PubChem CID:</color>** [[https://pubchem.ncbi.nlm.nih.gov/compound/5280961|5280961]]
    * **<color #00a2e8>IUPAC:</color>** %%5,7-dihydroxy-3-(4-hydroxyphenyl)chromen-4-one%%
    * **<color #00a2e8>INCHI Name:</color>** InChI=1S/C15H10O5/c16-9-3-1-8(2-4-9)11-7-20-13-6-10(17)5-12(18)14(13)15(11)19/h1-7,16-18H
    * **<color #00a2e8>INCHI Key:</color>** TZBJGXHYKVUXJN-UHFFFAOYSA-N
    * **<color #00a2e8>Molecular Weight:</color>** 270.24
    * **<color #00a2e8>Canonical SMILES:</color>** C1=CC(=CC=C1C2=COC3=CC(=CC(=C3C2=O)O)O)O
    * **<color #00a2e8>Isomeric SMILES:</color>** N/A
    * **<color #00a2e8>Molecular Formula:</color>** C15H10O5
{{:chemogenomics:structures:chem-0214.svg?nolink}}
</panel>


<panel title='Compound Supplier' collapsed='true'>
    * **<color #00a2e8>Supplier Name:</color>** Combi-Blocks
    * **<color #00a2e8>Catalog #:</color>** OR-2321
    * **<color #00a2e8>Lot #:</color>** L76364
</panel>


<panel title='Compound Characterization' collapsed='true'>
    * **<color #00a2e8>HRMS (ESI-TOF) m/z:</color>** (M+H)+ Calcd for C15H10O5 271.0601; found 271.06011
</panel>


<panel title='Dose Response Curve' collapsed='true'>
    * **<color #00a2e8>Platform ID:</color>** Genistein
    * **<color #00a2e8>Min:</color>** 0.3685; **<color #00a2e8>Max:</color>** 88.8447
{{:chemogenomics:dose_response:dr_429.png?nolink&500 |}}
\\ 
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|< 300px 100px 200px >|
^ IC   ^ Concentration (µM) ^
| IC10  |N/A    |
| IC20  |13.3899    |
| IC30  |15.7760    |
| IC40  |18.1027    |
| IC50  |20.6354    |
| IC60  |N/A    |
| IC70  |N/A    |
| IC80  |N/A    |
| IC90  |N/A    |
\\ 
</panel>
</accordion>
==== Screen Summary ====
    * **<color #00a2e8>Round</color>**: 07
    * **<color #00a2e8>Dose</color>**: 15µM
    * **<color #00a2e8>Days of incubation</color>**: 8
    * **<color #00a2e8>Doublings</color>**: 1.5
    * **<color #00a2e8>Numbers of reads</color>**: 20695605


==== Screen Results ====


^Sensitive/Resistant hits (FDR<0.05)^CRANKS^Score Plot^Top 30 Genes^Screen Similarity^Top 30 Sensitive GO terms^Top 30 Resistant GO terms^
|14/51|<color #e85500>[[https://files.tyerslab.com/files/public/chemogenomics/cranks/Genistein_15uM_Round-7_exp360.txt|Scores]]</color>|<button type="primary" size="sm" modal="ScorePlot">View</button>|<button type="primary" size="sm" modal="modal_hits">View</button>|<button type="primary" size="sm" modal="modal_screen_similarity">View</button>|<button type="primary" size="sm" modal="modal_go_sensitive">View</button>|<button type="primary" size="sm" modal="modal_go_resistant">View</button>|
<modal id='ScorePlot' size='lg' title='Genistein 15μM R07 exp360'>
{{:chemogenomics:cranks_plots:exp360.png?nolink}}
</modal>


<modal id="modal_hits" size="sm" title="Top 30 S/R Genes">
^Gene^CRANKS Score^FDR^
|[[:human_genes:Z:ZNF451|ZNF451]]|-3.92|<0.01|
|[[:human_genes:T:TSC2|TSC2]]|-3.31|<0.01|
|[[:human_genes:L:LIG4|LIG4]]|-3.06|<0.01|
|[[:human_genes:M:MCM9|MCM9]]|-3.04|<0.01|
|[[:human_genes:T:TCF4|TCF4]]|-3.02|<0.01|
|[[:human_genes:P:PDS5B|PDS5B]]|-3.00|<0.01|
|[[:human_genes:T:TDP2|TDP2]]|-2.98|<0.01|
|[[:human_genes:N:NHEJ1|NHEJ1]]|-2.81|<0.01|
|[[:human_genes:H:H2AFX|H2AFX]]|-2.79|0.01|
|[[:human_genes:A:ASB3|ASB3]]|-2.77|0.01|
|[[:human_genes:N:NPRL2|NPRL2]]|-2.76|<0.01|
|[[:human_genes:A:ATM|ATM]]|-2.69|0.01|
|[[:human_genes:T:TOP1|TOP1]]|-2.68|0.01|
|[[:human_genes:P:PSMA2|PSMA2]]|-2.67|0.09|
|[[:human_genes:P:PSMA7|PSMA7]]|-2.64|0.09|
|[[:human_genes:P:PBRM1|PBRM1]]|-2.60|0.01|
|[[:human_genes:N:NPRL3|NPRL3]]|-2.53|0.09|
|[[:human_genes:M:MSI2|MSI2]]|-2.51|0.06|
|[[:human_genes:P:PSMB7|PSMB7]]|-2.40|0.09|
|[[:human_genes:S:SWI5|SWI5]]|-2.36|0.09|
|[[:human_genes:L:LEF1|LEF1]]|-2.28|0.09|
|[[:human_genes:S:SMCHD1|SMCHD1]]|-2.26|0.09|
|[[:human_genes:M:MCM8|MCM8]]|-2.23|0.09|
|[[:human_genes:T:TP53BP1|TP53BP1]]|-2.23|0.09|
|[[:human_genes:R:RAD9B|RAD9B]]|-2.23|0.09|
|[[:human_genes:C:CNOT2|CNOT2]]|-2.22|0.09|
|[[:human_genes:P:PUS7|PUS7]]|-2.21|0.13|
|[[:human_genes:D:DCLRE1C|DCLRE1C]]|-2.21|0.09|
|[[:human_genes:U:UBE2N|UBE2N]]|-2.19|0.09|
|[[:human_genes:F:FAM35A|FAM35A]]|-2.17|0.09|
|[[:human_genes:N:NARS2|NARS2]]|2.53|0.01|
|[[:human_genes:G:GLMN|GLMN]]|2.53|0.01|
|[[:human_genes:P:PRKRIP1|PRKRIP1]]|2.54|0.01|
|[[:human_genes:K:KLF16|KLF16]]|2.58|0.03|
|[[:human_genes:F:FKBP5|FKBP5]]|2.59|0.01|
|[[:human_genes:C:CCDC41|CCDC41]]|2.60|0.02|
|[[:human_genes:P:PPP1R8|PPP1R8]]|2.62|0.02|
|[[:human_genes:D:DDX46|DDX46]]|2.63|0.26|
|[[:human_genes:Z:ZDHHC12|ZDHHC12]]|2.65|<0.01|
|[[:human_genes:C:CCNL1|CCNL1]]|2.67|<0.01|
|[[:human_genes:M:MTG1|MTG1]]|2.67|0.01|
|[[:human_genes:N:NFU1|NFU1]]|2.69|0.02|
|[[:human_genes:A:ATP1A1|ATP1A1]]|2.69|0.01|
|[[:human_genes:N:NDUFAF3|NDUFAF3]]|2.78|0.02|
|[[:human_genes:N:NDUFB11|NDUFB11]]|2.88|<0.01|
|[[:human_genes:G:GLRX5|GLRX5]]|2.89|<0.01|
|[[:human_genes:G:GPI|GPI]]|2.93|0.05|
|[[:human_genes:C:COPS7B|COPS7B]]|2.94|<0.01|
|[[:human_genes:W:WBP4|WBP4]]|2.98|<0.01|
|[[:human_genes:M:ME2|ME2]]|3.00|<0.01|
|[[:human_genes:P:PIGO|PIGO]]|3.07|<0.01|
|[[:human_genes:P:PQBP1|PQBP1]]|3.09|<0.01|
|[[:human_genes:N:NDUFB6|NDUFB6]]|3.13|<0.01|
|[[:human_genes:U:UBXN7|UBXN7]]|3.24|<0.01|
|[[:human_genes:N:NDUFA11|NDUFA11]]|3.29|<0.01|
|[[:human_genes:U:UBE2G1|UBE2G1]]|3.54|<0.01|
|[[:human_genes:E:ERCC6|ERCC6]]|3.91|<0.01|
|[[:human_genes:S:SLC25A51|SLC25A51]]|4.06|<0.01|
|[[:human_genes:T:TP53|TP53]]|4.32|<0.01|
|[[:human_genes:T:TOP2B|TOP2B]]|8.37|<0.01|
</modal>


<modal id="modal_screen_similarity" size="lg" title="Correlated Screens">
^Screen^Correlation^Plot^
|[[:results:exp329|Hydroxyurea 100μM R07 exp329]]|0.103|<button type="primary" size="small" modal="1">View</button>|
|[[:results:exp288|HMS-I2 10μM R06 exp288]]|0.099|<button type="primary" size="small" modal="2">View</button>|
|[[:results:exp240|Pyridostatin 4μM R05 exp240]]|0.098|<button type="primary" size="small" modal="3">View</button>|
|[[:results:exp25|Oligomycin-A 2μM R00 exp25]]|0.088|<button type="primary" size="small" modal="4">View</button>|
|[[:results:exp84|UM0125461 0.74μM R02 exp84]]|0.084|<button type="primary" size="small" modal="5">View</button>|
|[[:results:exp416|Tubacin 1.6μM R07 exp416]]|0.078|<button type="primary" size="small" modal="6">View</button>|
|[[:results:exp299|Talazoparib 0.006μM R06 exp299]]|0.074|<button type="primary" size="small" modal="7">View</button>|
|[[:results:exp529|Thimerosal 0.85μM R08 exp529]]|0.069|<button type="primary" size="small" modal="8">View</button>|
|[[:results:exp294|Nutlin-3A 1.6μM R06 exp294]]|0.069|<button type="primary" size="small" modal="9">View</button>|
|[[:results:exp78|Pterostilbene 16μM R02 exp78]]|0.068|<button type="primary" size="small" modal="10">View</button>|
|[[:results:exp517|Quercetin 20μM R08 exp517]]|0.063|<button type="primary" size="small" modal="11">View</button>|
|[[:results:exp160|Ribavirin 10 to 15μM on day4 R04 exp160]]|0.055|<button type="primary" size="small" modal="12">View</button>|
|[[:results:exp414|Tozasertib 0.1μM R07 exp414]]|0.054|<button type="primary" size="small" modal="13">View</button>|
|[[:results:exp520|Rucaparib 6.5μM R08 exp520]]|0.053|<button type="primary" size="small" modal="14">View</button>|
|[[:results:exp184|Ixabepilone 0.004 to 0.005μM on day4 R04 exp184]]|0.052|<button type="primary" size="small" modal="15">View</button>|
|[[:results:exp198|Etoposide 0.1μM R05 exp198]]|0.111|<button type="primary" size="small" modal="16">View</button>|
|[[:results:exp450|Artemisinin 50μM R08 exp450]]|0.107|<button type="primary" size="small" modal="17">View</button>|
|[[:results:exp342|Calcium Ionophore 0.4μM R07 exp342]]|0.076|<button type="primary" size="small" modal="18">View</button>|
|[[:results:exp312|2-Methoxyestradiol 0.55 to 1μM on day4 R07 exp312]]|0.067|<button type="primary" size="small" modal="19">View</button>|
|[[:results:exp460|BML-284 0.09μM R08 exp460]]|0.063|<button type="primary" size="small" modal="20">View</button>|
|[[:results:exp311|2-Methoxyestradiol 0.55 to 0.75μM  on day4 R07 exp311]]|0.062|<button type="primary" size="small" modal="21">View</button>|
|[[:results:exp66|BI-D1870 3.15μM R02 exp66]]|0.06|<button type="primary" size="small" modal="22">View</button>|
|[[:results:exp451|Atovaquone 15μM R08 exp451]]|0.058|<button type="primary" size="small" modal="23">View</button>|
|[[:results:exp357|Dorsomorphin 5μM R07 exp357]]|0.056|<button type="primary" size="small" modal="24">View</button>|
|[[:results:exp478|Doxorubicin 0.02μM R08 exp478]]|0.054|<button type="primary" size="small" modal="25">View</button>|
|[[:results:exp440|Aphidicolin 0.4μM R08 exp440]]|0.054|<button type="primary" size="small" modal="26">View</button>|
|[[:results:exp350|Deferoxamine 11μM R07 exp350]]|0.053|<button type="primary" size="small" modal="27">View</button>|
|[[:results:exp179|Combretastatin A4 0.002 to 0.003μM day4 R04 exp179]]|0.051|<button type="primary" size="small" modal="28">View</button>|
|[[:results:exp231|Epothilone-B 0.0015μM R05 exp231]]|0.051|<button type="primary" size="small" modal="29">View</button>|
</modal>


<modal id="1" size="lg" title="Genistein 15μM R07 exp360  vs  Hydroxyurea 100μM R07 exp329">
{{:chemogenomics:comparison_plots:exp329_vs_exp360.png?nolink |}}
</modal>

<modal id="2" size="lg" title="Genistein 15μM R07 exp360  vs  HMS-I2 10μM R06 exp288">
{{:chemogenomics:comparison_plots:exp288_vs_exp360.png?nolink |}}
</modal>

<modal id="3" size="lg" title="Genistein 15μM R07 exp360  vs  Pyridostatin 4μM R05 exp240">
{{:chemogenomics:comparison_plots:exp240_vs_exp360.png?nolink |}}
</modal>

<modal id="4" size="lg" title="Genistein 15μM R07 exp360  vs  Oligomycin-A 2μM R00 exp25">
{{:chemogenomics:comparison_plots:exp25_vs_exp360.png?nolink |}}
</modal>

<modal id="5" size="lg" title="Genistein 15μM R07 exp360  vs  UM0125461 0.74μM R02 exp84">
{{:chemogenomics:comparison_plots:exp360_vs_exp84.png?nolink |}}
</modal>

<modal id="6" size="lg" title="Genistein 15μM R07 exp360  vs  Tubacin 1.6μM R07 exp416">
{{:chemogenomics:comparison_plots:exp360_vs_exp416.png?nolink |}}
</modal>

<modal id="7" size="lg" title="Genistein 15μM R07 exp360  vs  Talazoparib 0.006μM R06 exp299">
{{:chemogenomics:comparison_plots:exp299_vs_exp360.png?nolink |}}
</modal>

<modal id="8" size="lg" title="Genistein 15μM R07 exp360  vs  Thimerosal 0.85μM R08 exp529">
{{:chemogenomics:comparison_plots:exp360_vs_exp529.png?nolink |}}
</modal>

<modal id="9" size="lg" title="Genistein 15μM R07 exp360  vs  Nutlin-3A 1.6μM R06 exp294">
{{:chemogenomics:comparison_plots:exp294_vs_exp360.png?nolink |}}
</modal>

<modal id="10" size="lg" title="Genistein 15μM R07 exp360  vs  Pterostilbene 16μM R02 exp78">
{{:chemogenomics:comparison_plots:exp360_vs_exp78.png?nolink |}}
</modal>

<modal id="11" size="lg" title="Genistein 15μM R07 exp360  vs  Quercetin 20μM R08 exp517">
{{:chemogenomics:comparison_plots:exp360_vs_exp517.png?nolink |}}
</modal>

<modal id="12" size="lg" title="Genistein 15μM R07 exp360  vs  Ribavirin 10 to 15μM on day4 R04 exp160">
{{:chemogenomics:comparison_plots:exp160_vs_exp360.png?nolink |}}
</modal>

<modal id="13" size="lg" title="Genistein 15μM R07 exp360  vs  Tozasertib 0.1μM R07 exp414">
{{:chemogenomics:comparison_plots:exp360_vs_exp414.png?nolink |}}
</modal>

<modal id="14" size="lg" title="Genistein 15μM R07 exp360  vs  Rucaparib 6.5μM R08 exp520">
{{:chemogenomics:comparison_plots:exp360_vs_exp520.png?nolink |}}
</modal>

<modal id="15" size="lg" title="Genistein 15μM R07 exp360  vs  Ixabepilone 0.004 to 0.005μM on day4 R04 exp184">
{{:chemogenomics:comparison_plots:exp184_vs_exp360.png?nolink |}}
</modal>

<modal id="16" size="lg" title="Etoposide 0.1μM R05 exp198  vs  Genistein 15μM R07 exp360">
{{:chemogenomics:comparison_plots:exp198_vs_exp360.png?nolink |}}
</modal>

<modal id="17" size="lg" title="Artemisinin 50μM R08 exp450  vs  Genistein 15μM R07 exp360">
{{:chemogenomics:comparison_plots:exp360_vs_exp450.png?nolink |}}
</modal>

<modal id="18" size="lg" title="Calcium Ionophore 0.4μM R07 exp342  vs  Genistein 15μM R07 exp360">
{{:chemogenomics:comparison_plots:exp342_vs_exp360.png?nolink |}}
</modal>

<modal id="19" size="lg" title="2-Methoxyestradiol 0.55 to 1μM on day4 R07 exp312  vs  Genistein 15μM R07 exp360">
{{:chemogenomics:comparison_plots:exp312_vs_exp360.png?nolink |}}
</modal>

<modal id="20" size="lg" title="BML-284 0.09μM R08 exp460  vs  Genistein 15μM R07 exp360">
{{:chemogenomics:comparison_plots:exp360_vs_exp460.png?nolink |}}
</modal>

<modal id="21" size="lg" title="2-Methoxyestradiol 0.55 to 0.75μM  on day4 R07 exp311  vs  Genistein 15μM R07 exp360">
{{:chemogenomics:comparison_plots:exp311_vs_exp360.png?nolink |}}
</modal>

<modal id="22" size="lg" title="BI-D1870 3.15μM R02 exp66  vs  Genistein 15μM R07 exp360">
{{:chemogenomics:comparison_plots:exp360_vs_exp66.png?nolink |}}
</modal>

<modal id="23" size="lg" title="Atovaquone 15μM R08 exp451  vs  Genistein 15μM R07 exp360">
{{:chemogenomics:comparison_plots:exp360_vs_exp451.png?nolink |}}
</modal>

<modal id="24" size="lg" title="Dorsomorphin 5μM R07 exp357  vs  Genistein 15μM R07 exp360">
{{:chemogenomics:comparison_plots:exp357_vs_exp360.png?nolink |}}
</modal>

<modal id="25" size="lg" title="Doxorubicin 0.02μM R08 exp478  vs  Genistein 15μM R07 exp360">
{{:chemogenomics:comparison_plots:exp360_vs_exp478.png?nolink |}}
</modal>

<modal id="26" size="lg" title="Aphidicolin 0.4μM R08 exp440  vs  Genistein 15μM R07 exp360">
{{:chemogenomics:comparison_plots:exp360_vs_exp440.png?nolink |}}
</modal>

<modal id="27" size="lg" title="Deferoxamine 11μM R07 exp350  vs  Genistein 15μM R07 exp360">
{{:chemogenomics:comparison_plots:exp350_vs_exp360.png?nolink |}}
</modal>

<modal id="28" size="lg" title="Combretastatin A4 0.002 to 0.003μM day4 R04 exp179  vs  Genistein 15μM R07 exp360">
{{:chemogenomics:comparison_plots:exp179_vs_exp360.png?nolink |}}
</modal>

<modal id="29" size="lg" title="Epothilone-B 0.0015μM R05 exp231  vs  Genistein 15μM R07 exp360">
{{:chemogenomics:comparison_plots:exp231_vs_exp360.png?nolink |}}
</modal>



<modal id="modal_go_sensitive" size="lg" title="Top 30 sensitive GO terms">
^GO Term^Fold Change^Genes^
|V(D)J recombination|168.14|LIG4,NHEJ1,ATM,LEF1,DCLRE1C|
|DNA repair complex|142.92|LIG4,NHEJ1,ATM,TP53BP1,DCLRE1C|
|somatic diversification of immune receptors via germline recombination within a single locus|69.72|LIG4,NHEJ1,ATM,LEF1,DCLRE1C|
|somatic cell DNA recombination|69.72|LIG4,NHEJ1,ATM,LEF1,DCLRE1C|
|double-strand break repair via nonhomologous end joining|68.06|LIG4,NHEJ1,ATM,TP53BP1,DCLRE1C|
|somatic diversification of immune receptors|58.34|LIG4,NHEJ1,ATM,LEF1,DCLRE1C|
|cellular response to ionizing radiation|51.20|LIG4,H2AX,ATM,SWI5,TP53BP1,RAD9B|
|response to ionizing radiation|34.39|LIG4,NHEJ1,H2AX,ATM,SWI5,TP53BP1,RAD9B,DCLRE1C|
|double-strand break repair|34.13|LIG4,MCM9,TDP2,NHEJ1,H2AX,ATM,SWI5,SMCHD1,MCM8,TP53BP1,DCLRE1C,UBE2N|
|regulation of TORC1 signaling|33.24|TSC2,NPRL2,ATM,NPRL3,UBE2N|
|double-strand break repair via homologous recombination|29.57|MCM9,H2AX,ATM,SWI5,MCM8,UBE2N|
|recombinational repair|28.82|MCM9,H2AX,ATM,SWI5,MCM8,UBE2N|
|DNA recombination|25.52|LIG4,MCM9,NHEJ1,H2AX,ATM,SWI5,LEF1,MCM8,DCLRE1C,UBE2N|
|cellular response to radiation|20.66|LIG4,H2AX,ATM,SWI5,TP53BP1,RAD9B|
|DNA repair|16.99|LIG4,MCM9,PDS5B,TDP2,NHEJ1,H2AX,ATM,SWI5,SMCHD1,MCM8,TP53BP1,RAD9B,DCLRE1C,UBE2N|
|DNA metabolic process|12.78|LIG4,MCM9,PDS5B,TDP2,NHEJ1,H2AX,ATM,TOP1,SWI5,LEF1,SMCHD1,MCM8,TP53BP1,RAD9B,DCLRE1C,UBE2N|
|DNA damage response|11.21|LIG4,MCM9,PDS5B,TDP2,NHEJ1,H2AX,ATM,SWI5,SMCHD1,MCM8,TP53BP1,RAD9B,DCLRE1C,UBE2N|
|response to radiation|11.15|LIG4,NHEJ1,H2AX,ATM,SWI5,TP53BP1,RAD9B,DCLRE1C|
|chromosome organization|10.39|LIG4,MCM9,PDS5B,ATM,TOP1,SMCHD1,MCM8,DCLRE1C|
</modal>
<modal id="modal_go_resistant" size="lg" title="Top 30 resistant GO terms">
^GO Term^Fold Change^Genes^
|mitochondrial respiratory chain complex I assembly|42.51|NDUFA11,NDUFB6,NDUFB11,NDUFAF3|
|NADH dehydrogenase complex assembly|42.51|NDUFA11,NDUFB6,NDUFB11,NDUFAF3|
|generation of precursor metabolites and energy|10.29|TP53,SLC25A51,NDUFA11,NDUFB6,ME2,GPI,NDUFB11|
</modal>