GSK-J4 1μM R07 exp362 [The Human Chemical-Genetic Interaction Map Project]

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====== GSK-J4 1μM R07 exp362 ======
==== Mechanism of Action ====
JMJD3/KDM6B, UTX/KDM6A histone demethylase inhibitor (H3K27me2/me3)
  * **<color #00a2e8>Class / Subclass 1:</color>** Gene Regulation / Epigenetic Inhibitor




==== Technical Notes ====
<accordion collapsed='true'>
<panel title='Compound References' collapsed='true'>
    * **<color #00a2e8>PubChem Name:</color>** %%GSK J4 HCl%%
    * **<color #00a2e8>Synonyms:</color>** N/A
    * **<color #00a2e8>CAS #:</color>** 1797983-09-5
    * **<color #00a2e8>PubChem CID:</color>** [[https://pubchem.ncbi.nlm.nih.gov/compound/71729974|71729974]]
    * **<color #00a2e8>IUPAC:</color>** %%ethyl 3-[[2-pyridin-2-yl-6-(1,2,4,5-tetrahydro-3-benzazepin-3-yl)pyrimidin-4-yl]amino]propanoate;hydrochloride%%
    * **<color #00a2e8>INCHI Name:</color>** InChI=1S/C24H27N5O2.ClH/c1-2-31-23(30)10-14-26-21-17-22(28-24(27-21)20-9-5-6-13-25-20)29-15-11-18-7-3-4-8-19(18)12-16-29;/h3-9,13,17H,2,10-12,14-16H2,1H3,(H,26,27,28);1H
    * **<color #00a2e8>INCHI Key:</color>** TYXWLTBYINKVNT-UHFFFAOYSA-N
    * **<color #00a2e8>Molecular Weight:</color>** 454
    * **<color #00a2e8>Canonical SMILES:</color>** CCOC(=O)CCNC1=CC(=NC(=N1)C2=CC=CC=N2)N3CCC4=CC=CC=C4CC3.Cl
    * **<color #00a2e8>Isomeric SMILES:</color>** N/A
    * **<color #00a2e8>Molecular Formula:</color>** C24H28ClN5O2
{{:chemogenomics:structures:chem-0215.svg?nolink}}
</panel>


<panel title='Compound Supplier' collapsed='true'>
    * **<color #00a2e8>Supplier Name:</color>** Sigma-Aldrich
    * **<color #00a2e8>Catalog #:</color>** SML0701
    * **<color #00a2e8>Lot #:</color>** 053M4601V
</panel>


<panel title='Compound Characterization' collapsed='true'>
    * **<color #00a2e8>HRMS (ESI-TOF) m/z:</color>** (M+H)+ Calcd for C24H27N5O2 418.22375; found 418.22478
</panel>


<panel title='Dose Response Curve' collapsed='true'>
    * **<color #00a2e8>Platform ID:</color>** GSK-J4
    * **<color #00a2e8>Min:</color>** -0.8548; **<color #00a2e8>Max:</color>** 90.3053
{{:chemogenomics:dose_response:dr_430.png?nolink&500 |}}
\\ 
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|< 300px 100px 200px >|
^ IC   ^ Concentration (µM) ^
| IC10  |N/A    |
| IC20  |0.5053    |
| IC30  |0.6250    |
| IC40  |0.7484    |
| IC50  |0.8887    |
| IC60  |N/A    |
| IC70  |N/A    |
| IC80  |N/A    |
| IC90  |N/A    |
\\ 
</panel>
</accordion>
==== Screen Summary ====
    * **<color #00a2e8>Round</color>**: 07
    * **<color #00a2e8>Dose</color>**: 1µM
    * **<color #00a2e8>Days of incubation</color>**: 8
    * **<color #00a2e8>Doublings</color>**: 6.3
    * **<color #00a2e8>Numbers of reads</color>**: 19363826


==== Screen Results ====


^Sensitive/Resistant hits (FDR<0.05)^CRANKS^Score Plot^Top 30 Genes^Screen Similarity^Top 30 Sensitive GO terms^Top 30 Resistant GO terms^
|7/0|<color #e85500>[[https://files.tyerslab.com/files/public/chemogenomics/cranks/GSK-J4_1uM_Round-7_exp362.txt|Scores]]</color>|<button type="primary" size="sm" modal="ScorePlot">View</button>|<button type="primary" size="sm" modal="modal_hits">View</button>|<button type="primary" size="sm" modal="modal_screen_similarity">View</button>|<button type="default" size="sm" modal="modal_go_sensitive">View</button>|<button type="primary" size="sm" modal="modal_go_resistant">View</button>|
<modal id='ScorePlot' size='lg' title='GSK-J4 1μM R07 exp362'>
{{:chemogenomics:cranks_plots:exp362.png?nolink}}
</modal>


<modal id="modal_hits" size="sm" title="Top 30 S/R Genes">
^Gene^CRANKS Score^FDR^
|[[:human_genes:C:C6orf136|C6orf136]]|-3.89|<0.01|
|[[:human_genes:A:ABCB7|ABCB7]]|-3.25|<0.01|
|[[:human_genes:Z:ZRANB2|ZRANB2]]|-2.98|<0.01|
|[[:human_genes:S:SLC30A1|SLC30A1]]|-2.89|<0.01|
|[[:human_genes:R:RSRC2|RSRC2]]|-2.86|0.24|
|[[:human_genes:A:ADO|ADO]]|-2.77|0.01|
|[[:human_genes:M:MTF1|MTF1]]|-2.68|0.05|
|[[:human_genes:P:PITRM1|PITRM1]]|-2.64|0.03|
|[[:human_genes:C:CLUH|CLUH]]|-2.57|0.06|
|[[:human_genes:R:RBM20|RBM20]]|-2.46|0.05|
|[[:human_genes:S:SLC25A20|SLC25A20]]|-2.42|0.06|
|[[:human_genes:R:RHOA|RHOA]]|-2.37|0.07|
|[[:human_genes:P:PYURF|PYURF]]|-2.36|0.07|
|[[:human_genes:R:RPE|RPE]]|-2.36|0.12|
|[[:human_genes:Z:ZCRB1|ZCRB1]]|-2.35|0.12|
|[[:human_genes:A:ADCK4|ADCK4]]|-2.32|0.12|
|[[:human_genes:T:TCF4|TCF4]]|-2.30|0.09|
|[[:human_genes:C:CENPA|CENPA]]|-2.29|0.13|
|[[:human_genes:F:FAM195A|FAM195A]]|-2.29|0.09|
|[[:human_genes:G:GCLC|GCLC]]|-2.24|0.12|
|[[:human_genes:C:C12orf75|C12orf75]]|-2.20|0.26|
|[[:human_genes:I:INO80C|INO80C]]|-2.19|0.12|
|[[:human_genes:P:PCYT2|PCYT2]]|-2.19|0.26|
|[[:human_genes:P:PARL|PARL]]|-2.18|0.21|
|[[:human_genes:S:SLC27A4|SLC27A4]]|-2.16|0.13|
|[[:human_genes:F:FOXP4|FOXP4]]|-2.15|0.22|
|[[:human_genes:V:VDAC1|VDAC1]]|-2.11|0.17|
|[[:human_genes:U:UHRF1|UHRF1]]|-2.09|0.24|
|[[:human_genes:H:HSPH1|HSPH1]]|-2.08|0.19|
|[[:human_genes:D:DDA1|DDA1]]|-2.04|0.21|
|[[:human_genes:P:PLXNB2|PLXNB2]]|1.95|0.54|
|[[:human_genes:R:RPS28|RPS28]]|1.96|0.72|
|[[:human_genes:M:MBD1|MBD1]]|1.97|0.55|
|[[:human_genes:N:NDUFB7|NDUFB7]]|1.97|0.55|
|[[:human_genes:A:AP2M1|AP2M1]]|1.99|0.55|
|[[:human_genes:C:CALD1|CALD1]]|2.00|0.55|
|[[:human_genes:A:APH1B|APH1B]]|2.00|0.46|
|[[:human_genes:H:HP1BP3|HP1BP3]]|2.01|0.46|
|[[:human_genes:I:INTS12|INTS12]]|2.09|0.31|
|[[:human_genes:E:EIF3K|EIF3K]]|2.09|0.31|
|[[:human_genes:R:RRN3|RRN3]]|2.10|0.31|
|[[:human_genes:O:OR4F5|OR4F5]]|2.14|0.55|
|[[:human_genes:S:SLC25A1|SLC25A1]]|2.15|0.28|
|[[:human_genes:C:CDC73|CDC73]]|2.17|0.55|
|[[:human_genes:P:PDS5B|PDS5B]]|2.19|0.28|
|[[:human_genes:P:POLA1|POLA1]]|2.21|0.72|
|[[:human_genes:D:DTX3|DTX3]]|2.24|0.49|
|[[:human_genes:C:CKAP5|CKAP5]]|2.24|0.55|
|[[:human_genes:F:FAU|FAU]]|2.24|0.55|
|[[:human_genes:P:POLE2|POLE2]]|2.25|0.55|
|[[:human_genes:P:POLR2H|POLR2H]]|2.25|0.28|
|[[:human_genes:F:FREM3|FREM3]]|2.26|0.28|
|[[:human_genes:C:CDK8|CDK8]]|2.28|0.28|
|[[:human_genes:D:DCAF17|DCAF17]]|2.28|0.28|
|[[:human_genes:K:KRTAP22-2|KRTAP22-2]]|2.39|0.26|
|[[:human_genes:R:RPS9|RPS9]]|2.63|0.55|
|[[:human_genes:C:COL19A1|COL19A1]]|2.64|0.21|
|[[:human_genes:S:SEC13|SEC13]]|2.64|0.28|
|[[:human_genes:A:ARID4B|ARID4B]]|2.65|0.21|
|[[:human_genes:P:POLA2|POLA2]]|2.66|0.28|
</modal>


<modal id="modal_screen_similarity" size="lg" title="Correlated Screens">
^Screen^Correlation^Plot^
|[[:results:exp248|UM0131023 0.05μM R05 exp248]]|0.055|<button type="primary" size="small" modal="1">View</button>|
|[[:results:exp421|VHL-ligand-1 20μM R07 exp421]]|0.053|<button type="primary" size="small" modal="2">View</button>|
|[[:results:exp363|GSK-J4 1-1.25μM to day4 R07 exp363]]|0.125|<button type="primary" size="small" modal="3">View</button>|
</modal>


<modal id="1" size="lg" title="GSK-J4 1μM R07 exp362  vs  UM0131023 0.05μM R05 exp248">
{{:chemogenomics:comparison_plots:exp248_vs_exp362.png?nolink |}}
</modal>

<modal id="2" size="lg" title="GSK-J4 1μM R07 exp362  vs  VHL-ligand-1 20μM R07 exp421">
{{:chemogenomics:comparison_plots:exp362_vs_exp421.png?nolink |}}
</modal>

<modal id="3" size="lg" title="GSK-J4 1-1.25μM to day4 R07 exp363  vs  GSK-J4 1μM R07 exp362">
{{:chemogenomics:comparison_plots:exp362_vs_exp363.png?nolink |}}
</modal>



<modal id="modal_go_sensitive" size="lg" title="Top 30 sensitive GO terms">No GO term hits below threshold. (FDR <0.05)</modal>
<modal id="modal_go_resistant" size="lg" title="Top 30 resistant GO terms">
^GO Term^Fold Change^Genes^
|RNA polymerase complex|22.79|POLA2,POLR2H,POLA1,CDC73|
|transferase complex, transferring phosphorus-containing groups|11.76|POLA2,CDK8,POLR2H,POLE2,POLA1,CDC73|
|nucleobase-containing compound biosynthetic process|6.64|POLA2,ARID4B,POLR2H,POLE2,POLA1,CDC73,SLC25A1,RRN3,NDUFB7,MBD1|
|heterocycle biosynthetic process|6.09|POLA2,ARID4B,POLR2H,POLE2,POLA1,CDC73,SLC25A1,RRN3,NDUFB7,MBD1|
|aromatic compound biosynthetic process|6.04|POLA2,ARID4B,POLR2H,POLE2,POLA1,CDC73,SLC25A1,RRN3,NDUFB7,MBD1|
</modal>